• Environmental Analysis Health and Toxicology
• /
• v.17 no.4
• /
• pp.325-332
• /
• 2002

A helper T(Th)1-mediated response is known to enhance cell -mediated immunity, while a Th2-mediated response is associated with the humoral immunity that if elevated IgE levels and eosinophilia. Prostaglandin (PG)E$_2$results in the decreased capability of Iymphocytes to produce Thl cytokines, with a shift toward a Th2 cytokine response. Chlorpyrifos (CPF) has been reported to impair the blastogenesis and response of T Iymphocytes. CPF also induces delayed febrile effects, which results from the activation of COX -PGE$_2$pathway. The purpose of this study is to determine the effort of CPF on the in vitro production of Th cytokines and the role of PGE$_2$on the CPF-induced production of Th cytokines. Splenocytes obtained from male BALB/c mice were pretreated with CPF(0.1, 1, 10 and 100$\mu$M) in the presence of absence of indomethacin or PGE$_2$for 12 h and then were incubated with concanavalin (Con) A for 48 h. These results showed that CPF remarkedly reduced the production of splenic interleukin (IL)-2 and interferon (IFN)-γ in a dose-dependent manner. CPF significantly increased the splenic IL-4 production at low doses (0.1 and 1$\mu$M) but did not affect at high doses (10 and 100 $\mu$M). Indomethacin reduced the CPF-decreased production of IL-2 and IFN-γ in a dose -dependent manner and significantly attenuated the production of IL-4 increased by CPF 0.1 $\mu$M. High dose of CPF significantly reduced the PGE$_2$-decreased production of IL-2 and IFN-γ, while the PGE$_2$- induced production of IL-4 was significantly enhanced by CPF 1 $\mu$M. These findings suggest that CPF nay down-regulate the immune response of Th 1 type by the suppressed production of IL-2 and IFN-γ, with a shift toward a Th2 cytokine response. The CPF-decreased production of Thl cytokines may not be mediated by endogenous PGE$_2$. Also, CPF may attenuate the exogenous PGE$_2$-decreased Th 1 immune response in a dose--dependent manner but may affect dose-independently the PGE$_2$-induced Th2 immune response.

• KSBB Journal
• /
• v.14 no.5
• /
• pp.528-533
• /
• 1999

Membrane-bound alcohol dehydrogenase(ADH) was purified to homogeneity from the acetic acid producing bacteria, Acetobacter sp. CS5. The enzyme was solubilized and extracted with Trition-X and purified using the DEAE-Sephacel chromatography and Sephacryl S-200 chromatography. The enzyme was purified to 14-fold with a yield of 15%. The molecular weight of the purified enzyme was to be 332 KDa. SDS-PAGE of the enzyme showed three subunits with molecular weights of 79 KDa, 49KDa and 46K Da. It indicated that enzyme consisted of three subunits of the 79 KDa, two subunits of the 49 KDa and. 46 KDa, respectively. The apparent Km value for ethanol was 0.77 mM and the optimum pH and temperature was 4.0-5.0 and 35$^{\circ}C$, respectively.

• International Journal of Automotive Technology
• /
• v.6 no.4
• /
• pp.323-332
• /
• 2005

A cylindrical constant volume combustion chamber was used to investigate the flow characteristics at the spark electrode gap and the combustion characteristics of a homogeneous charged methane-air mixture under various overall charge pressures, excess air ratios and ignition times. The flow characteristics, including the mean velocity and turbulence intensity, were analyzed with a hot wire anemometer. Combustion pressure development measured by piezoelectric pressure transducer, a flame propagation image acquired by ICCD camera and exhaust emissions measured by 2-valve gas chromatography were used to investigate effects of initial pressures, excess air ratios and ignition times on the combustion characteristics. It was found that the mean velocity and turbulence intensity had the maximum value around 200-300 ms and then decreased gradually to a near-zero value after 3000 ms and that the combustion duration was shorten and the flame speed and laminar burning velocity had the highest value under the condition of an excess air ratio of 1.1, an overall charge pressure of 0.15 MPa and an ignition time of 300 ms in the present study. The $CO_2$ concentration was proportional to the ignition time and overall charge pressure, the $CO_2$ concentration was proportional to the excess air ratio, and the UHC concentration was inversely proportional to the ignition time and overall charge pressure.

• Microbiology and Biotechnology Letters
• /
• v.44 no.3
• /
• pp.324-332
• /
• 2016

A bacterial strain capable of hydrolyzing xylan was isolated from fermented soybean paste obtained from a domestic Buddhist temple, using enrichment culture with rice straw as a carbon source. The isolate, named YB-1301, was identified as Bacillus safensis on the basis of its DNA gyrase subunit B gene (gyrB) sequence. The xylanase productivity of strain YB-1301 was drastically increased when it was grown in the presence of wheat bran or various xylans. In particular, the maximum xylanase productivity reached above 340 U/ml in the culture filtrate from LB broth supplemented with only birchwood xylan at shake-flask level. The xylanase production was significantly induced by xylans at the stationary growth phase in LB medium containing xylan, whereas only a small amount of xylanase was constitutively produced from cells grown in LB medium with no addition of xylan. Furthermore, xylanase biosynthesis was induced more rapidly by the enzymatically hydrolyzed products of xylan than by the non-hydrolyzed xylan. In addition, the xylanase in the culture filtrate of B. safensis YB-1301 was found to have optimal activity at 55℃ and pH 6.5–7.0.

• The Korean Journal of Physiology and Pharmacology
• /
• v.16 no.5
• /
• pp.327-332
• /
• 2012

Sertraline is a commonly used antidepressant of the selective serotonin reuptake inhibitors (SSRIs) class. In these experiments, we have used the whole cell patch clamp technique to examine the effects of sertraline on the major cardiac ion channels expressed in HEK293 cells and the native voltage-gated $Ca^{2+}$ channels in rat ventricular myocytes. According to the results, sertraline is a potent blocker of cardiac $K^+$ channels, such as hERG, $I_{Ks}$ and $I_{K1}$. The rank order of inhibitory potency was hERG > $I_{K1}$ > $I_{Ks}$ with $IC_{50}$ values of 0.7, 10.5, and 15.2 ${\mu}M$, respectively. In addition to $K^+$ channels, sertraline also inhibited $I_{Na}$ and $I_{Ca}$, and the $IC_{50}$ values are 6.1 and 2.6 ${\mu}M$, respectively. Modification of these ion channels by sertraline could induce changes of the cardiac action potential duration and QT interval, and might result in cardiac arrhythmia.

• The Korean Journal of Rheology
• /
• v.8 no.3_4
• /
• pp.199-206
• /
• 1996

산마와 재배마에서 점질다당류를 추출하여 리올러지 및 열특성을 연구하였다. D.I.W 에서 산마와 재배마 점질다당류의 고유점도(η)는 각각 16.1 이/g. 25.1 이/g 이고 pH7일 때 최대값을 나타내었고 염을 가했을 때 (η)가 급격히 감소하다가 일정한 값을 나타내었으며 urea 용액에서 NaCl, MaCl2 용액에서보다 (η)가 높았다. 마 점질다당류의 chain stiffness는 0,0050이고 Overlap parameter는 산마와 재배마 점질다당류가 각각 0.06 g/이. 0.04 g/이였 다. 마 점질 다당류는 shear rate가 증가함에 따라 겉보기 점도가 감소하여꼬 농도가 증가함 에따라 yield stress 와 점조성 지수는 증가하고 shear index가 감소하는 가소성유동체였다. 산마 점질다당류는 35$^{\circ}C$까지 온도에 대하여 비교적 안정하였지만 그이상의 온도에서는 겉보 기 점도가 급격히 감소하였고 재배마 점질다당류는 온도가 증가할수록 겉보기 점도가 점차 적으로 감소하였다. 겉보기 점도는 1가 염가 2가 염 사이에 큰 차이가 없었고 염농도가 증 가함에 따라 겉보기 점도가 감소하였으나 urea를 첨가했을 때 겉보기 점도가 약간 감소하다 가 다시 증가하는 것을 알수 있었다. DSC로 산마와 재배마 점질다당류의 "true melting"을 측정했을 때 각각 92$^{\circ}C$, 93$^{\circ}C$였고, enthalpy는 67.06 cal/g, 75.83 cal/g였다. 겔화에 소요된 enthalpy는 pH가 증가할수록 큰 값을 나타냈고 NaCl을 첨가했을 때 감소하다가 염농도 증 가에는 영향을 받지 않았으며 Urea와 glycerol을 각각 첨가했을 때 D. I. W.오세보다 감소 하였다. TGA로 열분해 현상을 측정했을 때 분해 온도는 산마와 재배와 점질다당류가 각각 219~332$^{\circ}C$, 229~341$^{\circ}C$ 범위였다.TEX> 범위였다.

• Journal of Preventive Medicine and Public Health
• /
• v.7 no.2
• /
• pp.327-332
• /
• 1974

Wells are perticularly able to be a source of water-borne infections disease in the army society. Owing to untreated drinking water, high indidence of water-borne diseases are encounted in Korea. This study was carried out to evaluate the physicochemical and bacteriological states of 298 wells in army camps around Daegu city during a year from January to November, 1974. Main findings are summerized as follows: 1. Turbidity, Colorness & odor was within normal range in all samples. 2. Reaction of pH, cnsumption of KMnO4, total hardness and chlorine ion as over almost within normal range. 3. Free Ammonia was detected to 33.2% positive, and nitrite, 45.5% positive. 4. In the bacteriological test 40.3% of all samples were over the standard limit on E. Coli. 5. In comparison of nitrate and E. Coli detection, 60.1% of nitrite was over the standard limit out of the positive cases detected E. Coli. and all of E. Coli were over the standard limit out of the positive cases of nitrite. 6. The contamination was very remarkable in the spring and summer, and very diminished in the autumn and winter.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.7
• /
• pp.938-943
• /
• 2002

This study was perfonned to detect polymorphisms of the two candidate genes, bovine BTN (Butyrophilin) and ST AT5a (Signal Transducers and Activators of Transcription) gene using 98 Holstein bulls' frozen semen, and to offer the basic information for QTL (Quantitative Trait Loci) analysis. Each BTN PCR product was digested with endonuclease restriction enzyme. The digested fragments of four BTN PCR products were observed as follows: 316,280, and 162 bp in BTN1, 568, 305 and 263 bp in BTN2, 576, 332, and 244 bp in BTN3, and 573, 291, and 282 bp in BTN4, respectively. The gene frequencies of A and B allele in four BTN loci were as follows: 0.8980 and 0.1020 in BTN1, 0.5510 and 0.4490 in BTN2, 0.8163 and 0.1837 in BTN3, and 0.8875 and 0.1122 in BTN4, respectively. And three genotypes (homotypel, heterotype, and homotype2) for STAT5a were observed by SSCP (single stranded conformational polymorphism) method and the genotype frequencies are 78.57%, 19.39%, and 2.04%, respectively. The PlC (Polymorphism Information Content) value and heterozygosity of four BTN loci were as follows: 0.1695 and 0.1870 in BTN1, 0.3713 and 0.4927 in BTN2, 0.2549 and 0.2999 in BTN3, and 0.1794 and 0.1992 in BTN4, respectively. Comparing with the reported data, PlC value of BTN2 might have the possibility to be useful marker. Other BTN loci indicated skewed allele distribution.

• Applied Chemistry for Engineering
• /
• v.19 no.3
• /
• pp.332-337
• /
• 2008

Hydrosilylation reaction of 1,1,1,3,5,5,5-heptamethyl trisiloxane (HMTS) and unsaturated poly(oxyethylene) (UPOE) was carried out in the presence of Speier's catalyst. The effect of molar ratio of two reagents on the reactivity and the structure of the product (HMTS-POE) were investigated by FT-IR and $^1H-NMR$. The pure HMTS-POE without containing unreacted HMTS or UPOE could be obtained by the reaction with excess amount of HMTS and followed by removing unreacted HMTS under vacuum. Under the same reaction conditions, various HMTS-POEs composed of UPOEs with a different molecular weight were synthesized, and the surface tensions of HMTS-POEs were analyzed. HMTS-POE, whose EO contents are in the range of 49~57%, exhibited lower surface tension and a potential application for low molecular weight silicone modified with polyethers.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.8 no.2
• /
• pp.211-220
• /
• 1998

The stochiometric composition of $AgGaS_2$polycrystal source materials for the single crystal thin films were prepared from horizontal furnace. From the extrapolation method of X-ray diffraction patterns, it was found that the polycrystal $AgGaS_2$has tetragonal structure of which lattice constant $a_0\;and \;c_0$ were 5.756 $\AA$ and 10.305 $\AA$, respectively. $AgGaS_2$single crystal thin film was deposited on throughly etched GaAs(100) substrate from mixed crystal $AgGaS_2$by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were $590^{\circ}C$ and $440^{\circ}C$ respectively, and the growth rate of the single crystal thin films was about 0.5 $mu \textrm{m}$/h. The crystallinity of the grown single crystal thin films was investigated by the DCRC (double crystal X-ray diffraction rocking curve). The optical energy gaps were found to be 2.61 eV for $AgGaS_2$single crystal thin films at room temperature. The temperature dependence of the photocurrent peak energy is well explained by the Varshni equation, then the constants in the Varshni equation are given by${\Alpha};=;8.695{\times}10^{-4};eV/K,and;{\beta};=;332;K$. from the photocurrent spectra by illumination of polarized light of the $AgGaS_2$single crystal thin film, we have found that crystal field splitting $\Delta$Cr was 0.28 eV at 20 K. From the PL spectra at 20 K, the peaks corresponding to free and bound excitons and a broad emission band due to D-A pairs are identified. The binding energy of the free excitons are determined to be 0.2676 eV and 0.2430 eV and the dissociation energy of the bound excitons to be 0.4695 eV.