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http://dx.doi.org/10.4014/mbl.1605.05004

Xylanase Production from Bacillus safensis Isolate by Xylan or Xylan Hydrolyzed Products  

Jin, Hyun Kyung (Food Science & Biotechnology Major, Woosong University)
Yoon, Ki-Hong (Food Science & Biotechnology Major, Woosong University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.3, 2016 , pp. 324-332 More about this Journal
Abstract
A bacterial strain capable of hydrolyzing xylan was isolated from fermented soybean paste obtained from a domestic Buddhist temple, using enrichment culture with rice straw as a carbon source. The isolate, named YB-1301, was identified as Bacillus safensis on the basis of its DNA gyrase subunit B gene (gyrB) sequence. The xylanase productivity of strain YB-1301 was drastically increased when it was grown in the presence of wheat bran or various xylans. In particular, the maximum xylanase productivity reached above 340 U/ml in the culture filtrate from LB broth supplemented with only birchwood xylan at shake-flask level. The xylanase production was significantly induced by xylans at the stationary growth phase in LB medium containing xylan, whereas only a small amount of xylanase was constitutively produced from cells grown in LB medium with no addition of xylan. Furthermore, xylanase biosynthesis was induced more rapidly by the enzymatically hydrolyzed products of xylan than by the non-hydrolyzed xylan. In addition, the xylanase in the culture filtrate of B. safensis YB-1301 was found to have optimal activity at 55℃ and pH 6.5–7.0.
Keywords
Bacillus safensis; induction; xylanase; xylan hydrolyzates;
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