• Title/Summary/Keyword: H.283

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Dipeptide (Tyr-Ile) Acting as an Inhibitor of Angiotensin-I-Converting Enzyme (ACE) from the Hydrolysate of Jellyfish Nemopilema nomurai

  • Kim, Yeon-Kye;Lim, Chi-Won;Yeun, So-Mi;Lee, Moon-Hee;Moon, Ho-Sung;Cho, Hyeon-Ah;Yoon, Na-Young;Yoon, Ho-Dong;Park, Hee-Yeon;Lee, Doo-Seog
    • Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.283-288
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    • 2011
  • The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using $C_{18}$ and $C_{12}$ columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The $IC_{50}$ and $K_i$ values were $6.56{\pm}1.12$ and $3.10{\pm}0.28\;{\mu}M$, respectively, indicating competitive inhibition of angiotensin-I-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.

Analysis-based Die Face Design for the Improvement of Surface Quality for a Heat Protect Panel of an Automobile (차량용 열차단판의 면품질 개선을 위한 성형해석 기반 금형면 설계)

  • Kim, K.P.;Kim, S.H.;Lee, D.G.;Jang, K.C.
    • Transactions of Materials Processing
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    • v.17 no.4
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    • pp.278-283
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    • 2008
  • This paper concerns the die face design for a heat protect panel aided by the finite element forming analysis in order to eliminate the surface defect and to improve the surface quality. The CAE procedure of the stamping process is introduced in order to reveal the reason of surface inferiorities and to improve surface quality. Complicated shape of the product induces the surface inferiorities such as wrinkling due to the insufficient restraining force of the forming blank and the non-uniform contact of the blank with the tools. This paper proposes a new guideline for the die design which includes the modification of tool shapes and addition of the draw-beads on the tool surface for ensuring the increased the restraining force with the uniform contact condition. The effectiveness of the proposed design is verified by the forming analysis and is confirmed by the tryout operation in the press shop. The analysis and test results show that the modified process parameters such as tool shapes and draw-beads can reduce the tendency of wrinkling and improve surface quality.

Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense

  • Han, Eun-Taek;Chen, Jun-Hu;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.46 no.4
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    • pp.279-283
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    • 2008
  • To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.

The Experimental Study on the Direct Synthesis of DME (Dimethyl Ether) in the Fixed Bed Reactor. (고정층 반응기에서 DME 직접합성에 관한 실험 연구)

  • Choi, Chang Woo;Cho, Wonihl;Ju, Woo-Sung;Lee, Seung-Ho;Baek, YoungSoon;Row, Kyung Ho
    • Journal of Hydrogen and New Energy
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    • v.15 no.4
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    • pp.283-290
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    • 2004
  • The single-step process for conversion of syngas to DME give higher conversion than the syngas-to-methanol process. This arises because of a synergy among the three simultaneous reaction, methanol synthesis, methanol dehydration and water gas shift reaction, in the process. we would find the optimal condition of the process which these advantages. The optimal condition of DME synthesis reaction over a commercial $Cu/Zn/Al_2O_3$ catalyst and Hybrid catalyst in a fixed bed reactor. The syngas-to-dimethyl ether conversion was examined on various reaction condition (Temperature 473~553K, $H_2/CO$ ratio 1~3, Pressure 30'50atm, GHSV 1000~4000).

Cloning and Characterization of a Glyoxalase I Gene from the Osmotolerant Yeast Candida magnoliae

  • Park, Eun-Hee;Lee, Dae-Hee;Seo, Jin-Ho;Kim, Myoung-Dong
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.277-283
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    • 2011
  • Glyoxalase I catalyzes the conversion of methylglyoxal to S-D-lactoylglutathione in the presence of glutathione. The structural gene of glyoxalase I (GLO1) was cloned from an osmotolerant yeast, Candida magnoliae, which produces a functional sweetener, erythritol, from sucrose. DNA sequence analysis revealed that the uninterrupted open reading frame (ORF) of C. magnoliae GLO1 (CmGLO1) spans 945 bp, corresponding to 315 amino acid residues, and shares 45.2% amino acid sequence identity to Saccharomyces cerevisiae Glo1. The cloned ORF in a multicopy constitutive expression plasmid complemented the glo1 mutation of S. cerevisiae, confirming that it encodes Glo1 in C. magnoliae. The responses of CmGLO1 to environmental stresses were different from those of S. cerevisiae, which only responds to osmotic stress. An enzyme activity assay and reverse transcription polymerase chain reaction revealed that the expression of CmGLO1 is induced by stress inducers such as methylglyoxal, $H_2O_2$, KCl, and NaCl. The GenBank Accession No. for CmGLO1 is HM000001.

A Comparative Study on the Bodice Patterns through Age Group for Women (성인여성을 위한 원형의 연령층별 비교연구)

  • 최미성;조훈정
    • Korean Journal of Human Ecology
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    • v.3 no.2
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    • pp.112-119
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    • 2000
  • The purpose of this study is to investigate the bodice patterns by analyzing sensory evaluations. Anthropomatric data was collected between April and October of 2000. Total 283 body measurements were collected for this study including both direct and indirect measurements(29 variables from the direct anthropometric data. 5 variables from the indirect anthropometric data). Data were analyzing using percentiles. standard deviation and Anova. The appearance and fit of three kinds of bodice patterns (N. L. H type) were evaluated by expert panel and the subjects. The results of the anthropometric measurements and sensory evaluations are as follows : A significant differences (p$\leq$.001) in the height, width and girth Items was found. The results of the bodice pattern evaluations by expert panel indicates that a significant difference (p${\le}$.05) in the responses to the placement of the waist dart, the fit of the bust area and the placement o( the back shoulder dart for 20's and 30's. The result of the evaluations by subjects indicates that a significant difference (p${\le}$.05) in the responses to the placement of the waist dart for 40's. A significant difference (p${\le}$.05) in the responses to the suitability of the shoulder area among the age groups.

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Estimation of PCDDs/PCDFs Intake for Korean Children and Their Mothers Through Daily Foods (음식물을 통한 어린이와 그들의 어머니에 대한 PCDDs/PCDFs 섭취량 평가)

  • Moon, Chan-Seok;Paik, Jong-Min
    • Journal of Environmental Health Sciences
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    • v.33 no.1 s.94
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    • pp.11-15
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    • 2007
  • This study was performed to evaluate the congeners of polychlorinated dibenzo-p-dioxins(PCDDS) and polychlorinated dibenzofurans(PCDFs) in Korean foods among children in Korea, in comparison with the findings in their mothers. The PCDDs and PCDFs intakes of 24-h diet duplicates were estimated from the previous Korean reports. In Korean children and their mothers of this study, major sources of lipid intake were plant-based foods(62% for children, 66% for their mothers). The women took 34% of lipid from animal sources, among which meats were leading sources of animal lipids(30%). Fish and shellfish were accounted for 4% in total lipid intake. Daily intake of PCDDs and PCDFs in the children md their mothers were 0.002-3.188 and 0.002-2.717 pg WHO-TEQ/day in animal sources and ND-0.283 and ND-0.296 pg WHO-TEQ/day in plant sources, respectively. PCDDs/PCDFs intake from animal origin was the major exposure source for both children and their mothers in Korea. Among the 17 PCDD/Fs congeners, 2,3,4,7,8-pentachlorodibenzofuran was assumed to be the effective exposure marker for diet intake.

Growth and Characterization of $CuInTe_2$ Single Crystal Thin Films by Hot Wall Epitaxy (Hot Wall Epitaxy (HWE) 법에 의한 $CuInTe_2$ 단결정 박막 성장과 가전자대 갈라짐에 대한 광전류연구)

  • Hong, Kwang-Joon;Park, Chang-Sun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.11a
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    • pp.156-159
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    • 2003
  • The stochiometric mixture of evaporating materials for the $CuInTe_2$ single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, $CuInTe_2$ mixed crystal was deposited on throughly etched GaAs(100) by the Hot Wall Epitaxy(HWE) system. The source and substrate temperature were $610^{\circ}C\;and\;450^{\circ}C$ respectively, and the growth rate of the single crystal thin films was about $0.5{\mu}m/h$. The crystalline structure of single crystal thin films was investigated by the double crystal X-ray diffraction(DCXD). From the photocurrent spectra, we have found that values of spin orbit coupling ${\Delta}So$ and crystal field splitting ${\Delta}Cr$ ware $0.283{\underline{3}}eV\;and\;0.120{\underline{0}}eV$, respectively.

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Psychological capital to foster employee creativity in nanotechnology companies: the mediating role of JS and CSR

  • Yuchun Li;Meilin Li;Xiangtong Kong;Arefeh Baniasadi;Ahmed Hasan Shaker;H. Elhosiny Ali
    • Advances in nano research
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    • v.15 no.3
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    • pp.277-283
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    • 2023
  • This research aims to explore factors influencing creativity among the employees of nanotechnology companies. Further, this survey aimed to investigate the role of psychological capital (PS), job satisfaction (JS) and corporate social responsibility (CSR) as sources to foster creativity. Participants included 375 employees of nanotechnology companies in China. Sequential mediation analysis revealed that creativity was significantly influenced by psychological capital, job satisfaction and corporate social responsibility. Furthermore, not only psychological capital, job satisfaction and corporate social responsibility were directly and indirectly related to creativity but also explained 67.35% variance of it. It seems that having more psychological resources, more well-being and good feelings concerning performance in the workspace and the efforts of companies to improve employee welfare are among the most important factors in increasing employee creativity. Our findings can help companies, especially nontechnology companies, in focusing on factors fostering the creativity of employees, because creativity enhances and promotes the performance and success of companies.

Comparative Proteome Analysis of Zerumbone-treated Helicobacter pylori (Zerumbone 처리에 따른 Helicobacter pylori의 단백질 비교분석)

  • Kim, Sa-Hyun
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.3
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    • pp.275-283
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    • 2018
  • Helicobacter pylori is a causative organism of various gastrointestinal diseases, including chronic gastritis, gastric ulcer, or gastric adenocarcinoma. Pathogenic factors, such as cytotoxin-associated protein A (CagA) and vacuolating cytotoxic protein A (VacA), play a role. This study analyzed qualitatively and quantitatively the effects of zerumbone on the changes in the protein expression levels of various H. pylori proteins, including CagA and VacA. Approximately 200 significant proteins were screened for the H. pylori 60190 (VacA positive / CagA positive; Eastern type) strain, and proteomic analysis was performed on 13 protein molecules that were clinically significant. After two-dimensional electrophoresis (2-DE), $ImageMaster^{TM}$ 2-DE Platinum software was used for quantitative measurements of protein spots. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-TOF-MS) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) were used for protein identification. After intensive analysis of the proteins that showed significant changes, a reverse transcription-polymerase chain reaction was performed as required to verify the results. In this study, the significance of zerumbone as a therapeutic agent for H. pylori infection was examined by screening a new pharmacological activity mechanism of zerumbone.