• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.4
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• pp.283-288
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• 2003

This study was carried out to a comparison of nutrient value and total digestible nutrient yield f3r select the promising barley cultivar for whole crop silage in paddy field of Suwon from 1998 to 2001. In milk stage, acid deferent fiber(ADF) content of barley were 27.3 ∼ 29.1, 27.4 ∼ 30.4 at yellow ripe stage. Neutral deferent fiber(NDF) content of milk and yellow ripe stage were 50.6 ∼ 55.9 and 53.1 ∼ 00.0. Also, total digestible nutrient(TDN) content of milk and yellow ripe stage we.e 65.4 ∼ 67.4 and 64.3 ∼ 67.7. And TDN yield at milk and yellow ripe stage were 4,120 ∼ 5,528 and 5,674 ∼ 6,569 MT per ha and in vitro dry matter digestibility(IVDMD) at milk and yellow ripe stage were 65.8 ∼ 74.6 and 64.6 ∼ 73.9. The for promising barley cultivars for whole crop silage in paddy field were Albori at Suwon. it was best before yellow ripe stage for harvest in cropping system.

• Korean Journal of Veterinary Research
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• v.60 no.4
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• pp.195-202
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• 2020

Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features of South Korean FCV are unclear. We aimed to investigate the biological and genetic features of South Korean FCV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FCV from 58 organ homogenate samples. The FCV isolates were confirmed by cytopathic effects, immunofluorescence, electron microscopy, and reverse transcription polymerase chain reaction assays. Viral genetic analysis was carried out with VP2 gene and complete genomes of FCVs. Five viruses propagated in CRFK cells were confirmed to be FCVs. The FCV17D283 isolate showed the highest viral titer of 107.2 TCID50/mL at 36 h post-inoculation. Korean FCV isolates did not grow well in Vero, BHK-21, A72, or Madin-Darby canine kidney cells. The FCV17D03 and FCV17D283 isolates had the highest genetic similarity (80.1% and 86.9%) with the UTCVM-H1 and 14Q315 strains, which were isolated in the United States and South Korea in 1995 and 2014, respectively. We isolated five FCVs from cats and detected important genetic differences among them. FCV isolates did not show any virulent effects in mice.

• Journal of Life Science
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• v.34 no.8
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• pp.576-587
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• 2024

In this study, diverse bacterial strains were isolated from fermented foods to screen those with antibacterial activity. Among them, one strain, identified as Bacillus vallismortis MCBL 1012 through 16S rRNA gene sequence analysis, was selected for its bacteriocin production. The culture supernatant of B. vallismortis MCBL 1012 showed antibacterial activity, mainly against Gram-positive bacteria. Scanning electron microscopy (SEM) revealed that bacteriocin treatment led to cellular content leakages in Listeria monocytogenes KCCM 40307, Enterococcus faecium KCCM 12118, and Streptococcus mutans KCTC 3065. PCR analysis confirmed B. vallismortis MCBL 1012 harbored subtilosin A gene (sbo A). Antibacterial activity was decreased by proteolytic enzymes like proteinase K, subtilisin A, and α-chymotrypsin. The bacteriocin demonstrated stability at 40℃ and 60℃ for 120 min, and up to 80℃ for 60 min, with rapid activity loss at 100℃. It retained full antibacterial activity within a pH range of 4.0 to 8.0 and was not affected by up to 100% organic solvents like ethanol, methanol, acetonitrile, and tetrahydrofuran. Nevertheless, activity decreased with more than 40% isopropanol and 80% acetone. Most tested inorganic salts and detergents had no effect on antibacterial activity except, CuSO₄ and NiSO₄ at specified concentrations. The bacteriocin exerted its antibacterial effect through bactericidal action against L. monocytogenes KCCM 40307. The bacteriocin was purified by ammonium sulfate precipitation, DEAE anion exchange chromatography, and RP-HPLC. The purification resulted in a final yield of 0.03% and a 283.7-fold increase in specific activity. MALDI-TOF MS analysis determined the exact molecular weight of purified bacteriocin to be 3,326.1 Da.

• Textile Coloration and Finishing
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• v.23 no.4
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• pp.274-283
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• 2011

Effect of pH on ozone oxidation and peroxone AOP(Advanced Oxidation Process) process was analyzed and the optimal efficiency for both processes was obtained at pH 7.5. In case of ozone oxidation process, the efficiencies of color, $COD_{Mn}$ and $BOD_5$ removal were measured to 93%, 70% and 89% at a reaction time of 50 min(ozone dosage of 111.67mg/$\ell$). When reaction time increased to 90 min(ozone dosage of 201mg/$\ell$), the efficiencies of color, $COD_{Mn}$ and $BOD_5$ removal were increased by 3~5 %, indicating that the increment of removal efficiency was insignificant considering longer reaction time. Similarly, the ozone/$H_2O_2$ ratio was optimized to 0.5 for peroxone AOP process. Removal efficiencies of color, $COD_{Mn}$ and $BOD_5$ were measured 95%, 81% and 94% at a reaction time of 50 min(ozone dosage of 111.67mg/$\ell$). When reaction time increased to 90min(ozone dosage of 201mg/$\ell$), the removal efficiency of color, CODMn, and BOD5 increased slightly by 1~5%.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.8 no.4
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• pp.283-289
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• 2014

This paper presents a design and optimization of the fully-active transfemoral prosthesis leg system. As it has one degree of freedom in knee joint, this prosthesis leg can imitate the human's gait. The weight of system, which makes the users more comfortable due to less tiredness, and the knee joint torque to rise stability of the system are major factors of prosthesis leg system. Thus the mechanism of prosthesis changes from 3-linkage type to geared type. The sensorized foot is also designed to effectively determine human's gait by measuring deformation of the foot during gait. Topology optimization is carried out for the sensorized foot to remove its unnecessary weight. The safety of optimized foot is verified by carrying out finite element analysis.

• Journal of Korean Society of Water and Wastewater
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• v.11 no.2
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• pp.112-127
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• 1997

The purpose of this study is to get optimum operating factors of Upflow Anaerobic Sludge Blanket (UASB) reactor by introducing methods that make it to reduce inhibition possible in each process wastewater treatment. The used substrates, concentrated corn starch liquid (CSL) wastewater, modified starch, filtering and decoloring wastewater, ion refining wastewater, and mixed wastewater including modified starch and not including modified starch, are generated from molasses process. The seeding sludge is the digested sludge that had been applied to molasses wastewater. Batch test to reduce the inhibition factors that might be existed in each wastewater was examined. Based on the this test, the optimum operating factors according to alkalinity and pH variation was studied through the continuous test using three 5.5 L UASB reactor. The first reactor added $NaHCO_3$ to control alkalinity. The hydraulic retention time (HRT) reduced to 8 hours and the organic loading rate increased gradually. The second reactor changed the pH of influent from 7.0 to 6.0 using NaOH. The third reactor was operated without changes to compare the above two reactors. As the result, the inhibition in concentrated CSL wastewater was removed by adding iron (II). When trace metals were added to mixed wastewater not including modified starch, the digestability by gas production rate increased to more fifty percentage than mixed wastewater that was not adding the trace metals. The reason that the inhibition did not decreased in spite of adding trace metals and nutrients was influenced by high concentration generated during the acid fermentation. The UASB reactors using the mixed wastewater with the most effective performance were operated as 500 mg/L as $CaCO_3$ alkalinity and 6.0 pH at steady state, and at this time, the gas production rates were 283 and 311mL gas/g $COD_{added}$. The COD removal rates were 84.7 and 86.3%, respectively.

• Korean journal of applied entomology
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• v.58 no.4
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• pp.283-289
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• 2019

Overuse of antibiotics has significantly contributed to an increase in microbial antibiotic resistance, causing difficulties in the suppression of microbe-borne infectious diseases. In this study, we determined the anti-Klebsiella pneumoniae effect in the kidneys of mice induced by peptides isolated from H. illucens larvae. Mice were intranasally infected with a high dose of K. pneumoniae and 1 day later, peptides were introduced through the intramuscular route. Mice were sacrificed on day 10 upon K. pneumoniae infection to determine the bacterial loads in the kidneys. Mice receiving peptide treatment demonstrated significantly reduced bacterial loads, reduced bodyweight loss, and higher survival in a dose-dependent manner compared to control. These results indicate that peptide isolated from H. illucens larva inhibits K. pneumoniae infection in the kidney. The peptide from H. illucens larva could be a potential candidate for the development of an effective antibacterial drug.

• Journal of Veterinary Clinics
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• v.30 no.4
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• pp.283-287
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• 2013

Canine skin is a highly dynamic organ that is constantly adapted to changes in its environment. It provides structural, sensory, immunologic, and physiologic functions and contributes an essential barrier function against potential environmental insults. We assessed the changes of canine skin hydration, pH, and protein contents by noninvasive method according to the frequency of application with canine commercial shampoo. Canine commercial shampoo was applied topically every other day, 5 days, and 8 days on different sites, respectively in 8 dogs. Saline-applied site was as a control. Skin hydration was increased and skin surface pH was decreased significantly in canine commercial shampoo-applied site every other day (p < 0.05). Also, skin protein concentration measured by D-squame$^{(R)}$ tape stripping method was increased significantly in canine commercial shampoo-applied site every other day (p < 0.05). There were alterations on every 5 days and 8 days, but not significantly. These results may be recognized that the frequent use of canine commercial shampoo cause abnormalities of the skin barrier function and alteration of stratum corneum integrity/cohesion. Consequently it was suggested that bathing the dog with canine commercial shampoo was appropriate every 5 or 8 days.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.283-287
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• 1994

The effects of packaging methods on the storage properities of salted Chinese cabbage were examinined during 3-week storage. The weights of salted Chinese cabbage decreased to 69-77% by water loss and salinities decreased from 2.6% to 2.0%, respectively. The pH decreased from 6.4 to 6.2 for 1- week storage in all cases, but the pH increased to 6.6 in non packaging(Non-P), maintained at 6.2 in PVC container packaging(PVC), and decreased continuously to 5.5 and 5.7 in LDPE packaging (LDPE) and HDPE packaging(HDPE), repectively for 3-week storage. Reducing sugars increased slightly for 1-week storage for the relative concentration effect by water loss, but decreased to 1.8% for 3-week storage because of the microbial fermentation. Cutting force increased in LDPE for the continuous osmosis phenomena but decreased in Non-P for the putrefaction. As the results, LDPE was found to be the most effective packaging method.

• Food Science and Preservation
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• v.10 no.3
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• pp.278-283
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• 2003

Addition of ethanol and/or polylysine to kimchi was investigated to improve its microbial hygienic quality and to extend shelf-life. Ethanol was added to kimchi with several concentrations(0.3％, 0.6％, 0.9％) and stored at 10$^{\circ}C$. Addition of 0.6％ and 0.9％ ethanol showed apparent inhibitory effect on growth of microorganism, but any distinct difference was not found between those concentrations. Addition of ethanol was more effective on growth inhibition of coliform and lactic acid bacteria than others. Addition of 0.6％ and 0.9％ ethanol retarded apparently pH decrease and acidity increase. Although addition of 0.6％ ethanol in combination with 0.12％ polylysine showed good retardation of pH decrease and acidity increase, overall organoleptic quality was not good because of off-flavor and taste. Also, addition of 0.6％ ethanol showed good overall organoleptic quality.