• Proceedings of the Korean Aquaculture Society Conference
• /
• 2003.10a
• /
• pp.56-57
• /
• 2003

실고기과(Syngnathidae)에 속하는 해마류는 5300만년전부터 지구상에 존재해온 어류로 전 세계적으로 72%에 해당하는 32종이 인도-태평양연안에서 서식하는 것으로 알려져 있다. 그리고 약제와 관상무역에서 넓은 시장을 형성하며 그 수요가 지속적으로 증가하고 있다. 그러나, 국내에서는 아직 해마의 서식지와 초기생활사에 대한 연구가 전무한 실정으로 이번 연구에서는 최근 발견된 한국산 해마의 서식지와 채집된 2종 해마의 치어 초기생활사를 보고한다. 2003년 8~9월에 전라남도 여수시와 고흥군 주변해역에서 해마, Hippocampus coronatus 18마리(전장 40.8~79.5mm), 산호해마 H, japonicus 80마리(전장 28.1~56.3mm)가 채집되었고, 순환여과식 사육수조에서 사육하면서 자연산란한 치어를 대상으로 초기생활사를 관찰하였다. 사육은 유리로 된 사각수조(50$\times$30$\times$28cm)에 여과조(26$\times$26$\times$28cm)를 연결한 순환여과시스템에서 실시하였으며, 사육수온은 26.0$\pm$0.5$^{\circ}C$, 염분 32$\textperthousand$ 전후, pH 7.74 전후 그리고 DO 6.8mg/$\ell$ 전후였다. 먹이는 부화 2일령까지는 로티퍼, Brachionus rotundiformis를 단독 급이하였고, 부화 후 3일령부터는 로티퍼와 알테미아, Artemia sp.를 혼합 급이하였다. 치어의 성장 측정은 만능투영기를 이용하여 각 20마리를 무작위 추출하여 측정하였고 각 측정 부위별로 0.001mm까지 측정하였다. 이번 조사에서 전라남도 여수시와 고흥군 주변해역에서 채집된 해마류는 해마(H. coronatus) 18개체, 산호해마(H. japonicus) 80개체의 2종이 채집되었고 순환여과시스템에 사육하던 중 5회 산란하였다. 해마(H. coronatus)는 부화 후 9~10일째에 Total length 11.67~15.98mm(평균 5.19mm, n=20), Trunk length 2.48~3.31mm(평균 2.63mm, n=20), Head length 1.31~2.27mm(평균 1.76mm, n=20) 그리고 Snout length 0.74~l.67mm(평균 1.09mm, n=20) 이었다. 한편, 부화 직후 산호해마(H. japonicus)의 치어는 난황을 거의 흡수한 상태였으며 부화 후 2~3일째 Total length 4.29~5.70mm(평균 5.09mm, n=30), Trunk length 2.06~2.73mm(평균 2.55mm, n=30), Head length 1.50~l.79mm(평균 1.06mm, n=30) 그리고 Snout length 0.81~l.14mm(평균 0.97mm, n=30)였다. 그리고 부화 후 5~6일째에는 Total length 4.33~6.33mm(평균 5.19mm, n=20), Trunk length로 2.48~3.31mm(평균 2.63mm, n=20), Head length 1.31~2.27mm(평균 1.76mm, n=20) 그리고 Snout length 0.74~l.67mm(평균 1.09mm, n=20) 이었다.

• Environmental Engineering Research
• /
• v.23 no.2
• /
• pp.195-204
• /
• 2018

A pilot scale study was conducted on a down-flow hanging sponge (DHS) reactor installed at a sewage treatment plant in Banda Aceh, Indonesia for treatment of desludging septic tank wastewater. Raw wastewater with an average biochemical oxygen demand (BOD) and total suspended solids of 139 mg/L and 191 mg/L, respectively, was pumped into the reactor. Two different hydraulic retention times (HRTs, 3 h and 4 h) were investigated, equivalent to organic loadings of 1.11 and $0.78kg\;BOD/m^3/d$, respectively. The average BOD concentration in the final effluent was 46 and 26 mg/L at HRTs of 3 and 4 h, respectively. The concentration of retained sludge along the reactor height was 10.2-18.7 g VSS/L-sponge, and the sludge activities were 0.24-0.32 and 0.04-0.40 mg/g VSS/h for heterotrophs and nitrification, respectively. Values of water hold-up volume, dispersion coefficient, and number of tank in-series found from tracer studies of clean sponge and biomass-loaded sponge confirmed that growth of retained sludge on the sponge module improved hydraulic performance of the reactor. Adoption of the DHS reactor by this Indonesian sewage treatment plant would enhance the role of the current desludging septic tank wastewater treatment system.

• Journal of Environmental Science International
• /
• v.16 no.12
• /
• pp.1425-1430
• /
• 2007

This study was conducted to investigate the effect of ozonated water and ozonated water+hydrogen peroxide treatment of residual procymidone in perilla leaf containing 20 mg/L procymidone. Samples was treated with ozonated water containing 1.0, 2.0 and 3.0 mg $O_3/L$ ozone and hydrogen peroxide water containing 1.0, 2.0 and 3.0 mg $H_2O_2/L$ hydrogen peroxide in pH 5, 7 and 9, respectively, at $15^{\circ}C$. Procymidone removal rate was 26.5% in 7 days at $15^{\circ}C$ and optimum condition of procymidone removal was the case of treating with ozonated water containing 2.0 mg $O_3/L$ and pH 9. As the result procymidone removal rate was about 96.5%. In this case of adding hydrogen peroxide, optimum condition of procymidone removal was $1:0.5{\sim}1(O_3:H_2O_2)$. However, procymidone was nearly removed with the treatment of hydrogen peroxide water only.

• Mycobiology
• /
• v.51 no.4
• /
• pp.230-238
• /
• 2023

Glabridin is a well-known active isoflavone found in the root of licorice (Glycyrrhiza glabra L.) that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytes cultures are the most important alternative methods for plant resources conservation and sustainable production of natural compounds, which has received much attention in recent decades. In the present study, an efficient culture condition was optimized for the biomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticola SBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licorice root extract (as an elicitor) were tested. The results showed that the highest and lowest biomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt (5.85 + 0.11 g/l), respectively. The medium culture PCB was produced the highest level of glabridin (7.26 ± 0.44 mg/l), while the lowest level (4.47 ± 0.02 mg/l) was obtained from the medium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51 mg/l) production were observed from the PCB medium adjusted with pH = 6, while the lowest value of both traits was obtained from the same medium with pH = 7. The highest production of total glabridin (10.85 ± 0.84 mg/l) was also obtained from the culture medium treated with 100 mg/l of the plant root extract. This information can be interestingly used for the commercialization of glabridin production for further industrial applications.

• Bulletin of the Korean Chemical Society
• /
• v.26 no.8
• /
• pp.1251-1254
• /
• 2005

It has been proposed that oxidation of L-3,4-dihydroxyphenylalanine (DOPA) may contribute to the pathogenesis of neurodegenerative disease. In this study, L-DOPA-Fe(III)-mediated DNA cleavage and the protection by carnosine and homocarnosine against this reaction were investigated. When plasmid DNA was incubated with L-DOPA in the presence of Fe(III), DNA strand was cleaved. Radical scavengers and catalase significantly inhibited the DNA breakage. These results suggest that $H_2O_2$ may be generated from the oxidation of DOPA and then $Fe^{3+}$ likely participates in a Fenton’s type reaction to produce hydroxyl radicals, which may cause DNA cleavage. Carnosine and homocarnosine have been proposed to act as anti-oxidants in vivo. The protective effects of carnosine and homocarnosine against L-DOPA-Fe(III)-mediated DNA cleavage have been studied. Carnosine and homocarnosine significantly inhibited DNA cleavage. These compounds also inhibited the production of hydroxyl radicals in L-DOPA/$Fe^{3+}$ system. The results suggest that carnosine and homocarnosine act as hydroxyl radical scavenger to protect DNA cleavage. It is proposed that carnosine and homocarnosine might be explored as potential therapeutic agents for pathologies that involve damage of DNA by oxidation of DOPA.

• Ocean and Polar Research
• /
• v.26 no.2
• /
• pp.333-339
• /
• 2004

This paper is concerned with an experimental investigation about tractive performance of a tracked vehicle on extremely soft soil. A tracked vehicle model with principal dimensions of $0.9\;m(L)\;{\times}\;0.75\;m(B)\;{\times}\;0.4\;m(H)$ and the weight of 167 kg was constructed with a pair of driving chain links driven by two AC-servo motors. The tracks are configured with detachable grousers with variable span. Deep seabed was simulated by means of bentonite-water mixture in a soil bin of $6.0\;m(L)\;{\times}\;3.7\;m(B)\;{\times}\;0.7\;m(H)$. Slip of vehicle and driving torque of motor were measured with respect to experimental variables; grouser span, grouser chevron angle, driving speed, drawbar-pull weight, position of center-of-gravity and weight. $L_8$ orthogonal array is adopted for DOE (Design Of Experiment). The effects of experiment variables on traction performance are evaluated.

• Korean Journal of Microbiology
• /
• v.26 no.4
• /
• pp.348-354
• /
• 1988

A Protozoa, Tetrahymena sp. was isolated from domestic wastewater and identified by morphological characteristics. It was 50 to $70{\mu}m$ in length, ovoidal shape, and divided by binary fission. When Escherchia coli was fed as a food microorganism, the optimal conditions for growth were 30 to $35^{\circ}C$, pH 7 to 9 and 0.05% NaCl. The growth was inhibited by heavy metal ions; 0.3 mg of $Cd^{++}/l$ and 0.1mg of $Cu^{++}/l$. While, it was not inhibited up to 0.7 mg of $Zn^{++}/l$.

• KSBB Journal
• /
• v.26 no.4
• /
• pp.328-332
• /
• 2011

Ethanol production using glycerol as a carbon source was performed by Enterobacter aerogenes immobilized on calcium alginate beads. To improve the ethanol production, the optimal conditions such as loading amount of immobilized cells and glycerol concentration were investigated. The optimal loading amount of immobilized cells and glycerol concentration were 10 mL of calcium alginate bead and 10 g/L, respectively. Consequently, glycerol consumption rate, ethanol concentration and yield were 0.32 g/$L{\cdot}h$, 3.38 g/L and 0.43 g/g on the batch production, respectively. Continuous production of ethanol was successfully achieved using two types of immobilized cell reactors (continuous stirred tank reactor and packed bed reactor) from 10 g/L of glycerol. In the continuous stirred tank reactor, glycerol consumption, ethanol concentration, specific productivity and yield were 9.8 g, 4.67 g/L, 1.17 g/$L{\cdot}h$, 0.48 g/g, respectively. The concentration of produced ethanol was 38-44% higher comparison to batch fermentation, and continuous stirred tank reactor showed better performance than packed bed reactor.

• Bulletin of the Korean Chemical Society
• /
• v.26 no.10
• /
• pp.1529-1532
• /
• 2005

A simple and cost effective method for separation and preconcentration of Ag(I) at the $10^{-7}\;mol\;L^{-1}$ level in the environmental and mineral samples is present. The method is based on the flotation of Ag(I)-iodide complex as an ion-associate with ferroin in pH of 4 from a large volume of an aqueous solution (500 mL) using nheptane. The floated layer was then dissolved in dimethylsulfoxide (DMSO) for the subsequent spectrophotometric determination. Beer's law was obeyed over a range of 2.0 ${\times}$ $10^{-7}$-4.0 ${\times}$ $10^{-6}$ mol $L^{-1}$ with the apparent molar absorptivity of 2.67 ${\times}$ $10^5$ L $mol^{-1}\;cm^{-1}$. The detection limit (n = 5) was 4 ${\times}$ $10^{-8}$ mol $L^{-1}$, and RSD (n = 5) obtained for 2.0 ${\times}$ $10^{-6}$ mol $L^{-1}$ of Ag(I) was 2.2%. The interference effects of a number of elements was studied and found that only $Hg^{2+}$ at low concentration, and $Pb^{2+}$, $Cd^{2+}$, $Cu^{2+}$, and $Fe^{3+}$ ions at moderately high concentrations were interfered. To overcome on these interference effects, the solution was treated with EDTA at a buffering pH of 4 and passed through a column containing Amberlite IR-120 ionexchanger resin, just before the flotation process. The proposed method was applied to determine of Ag(I) in a synthetic waste water, a photographic washing sample and a geological sample and the results was compared with those obtained from the flame atomic absorption spectrometry. The results were satisfactorily comparable with together, so that the applicability of the proposed method was confirmed in encountering with the real samples.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.8
• /
• pp.977-982
• /
• 2009

This study was carried out to investigate physiological activities in the extract of Eriobotrya japonica in order to elevate its utilization as a functional material. The maximum total polyphenol and total flavonoid contents were 28.91$\pm$2.1 mg/g and 10.54$\pm$4.6 mg/g, respectively. The nitrite scavenging abilities at pH 1.2 were in the range of $49.52\pm1.04\sim72.52\pm$0.84% whereas they were $35.28\pm0.78\sim51.20\pm$1.10% at pH 4.2 and $27.90\pm0.36\sim32.26\pm$1.20% at pH 6.0. At the concentrations of 100, 200 and 300 $\mu g$/mL of the hot-water extract, the degranulation of RBL-2H3 cells were inhibited 14.58$\pm$0.97%, 43.69$\pm$0.96% and 95.58$\pm$0.75%, respectively. The anti-inflammation activity was the highest as 44.35$\pm$0.63% at a concentration of 400 $\mu g$/mL.