• Preventive Nutrition and Food Science
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• v.21 no.3
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• pp.197-201
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• 2016

Helicobacter pylori infection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities of Centella asiatica leaf extract (CAE) against H. pylori both in vitro and in vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains of H. pylori were tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibiting H. pylori growth was 2 mg/mL. The anti-H. pylori effects of CAE in vivo were also examined in H. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reduced H. pylori colonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE against H. pylori infection, and it suggests that CAE may be useful as an alternative therapy.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.05a
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• pp.89-90
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• 2003

Extracts of Artemisia asiatica Nakai (Asteraceae) have been shown to have anti-inflammatory and anti-oxidative activities. Eupatilin (5,7-dihydroxy-3,4,6-tri-methoxy-flavone), one of the pharmacologically active ingredients derived from Artemisia asiatica, has been shown to induce apoptosis in promyelocytic leukemia (HL-60) cells. (omitted)

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.572-580
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• 1994

The purpose of the present study was to evaluate the effect of platelet - derived growth factor(PDGF) - BB and insulin - like growth factor(IGF) - 1, Centella Asiatica, and Zea Mays L. on the mitogenic activity of PDL cells from healthy and RPP patients. Combination of PDGF - BB and IGF - 1, Centella Asiatica, and Zea Mays L. were treated on PDL cells and the mitogenic effects were meaured by quantitative assay of methyl - $^3H$ - thymidine incorporation during DNA synthesis. Combination of PDGF - BB and IGF - 1 enhenced the mitogenic effects of both healthy and RPP PDL cells, however, the effect was less pronounced on RPP PDL cells. In cases of Centella Asiatica and Zea Mays L., no mitogenic effect on healthy PDL cells could be noticed.

• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.469-477
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• 2018

Hanabusaya asiatica is an endemic species whose distribution is limited in the mid-eastern part of the Korean peninsula. Due to its narrow range and small population, it is necessary to protect its habitats by identifying it as Key Biodiversity Areas (KBAs) adopted by the International Union for Conservation of Nature (IUCN). In this paper, we estimated potential natural habitats for H. asiatica using maximum entropy model (MaxEnt) and identified candidate sites for KBA based on the model results. MaxEnt is a machine learning algorithm that can predict habitats for species of interest unbiasedly with presence-only data. This property is particularly useful for the study area where data collection via a field survey is unavailable. We trained MaxEnt using 38 locations of H. asiatica and 11 environmental variables that measured climate, topography, and vegetation status of the study area which encompassed all locations of the border region between South and North Korea. Results showed that the potential habitats where the occurrence probabilities of H. asiatica exceeded 0.5 were $778km^2$, and the KBA candidate area identified by taking into account existing protected areas was $1,321km^2$. Of 11 environmental variables, elevation, annual average precipitation, average precipitation in growing seasons, and the average temperature in the coldest month had impacts on habitat selection, indicating that H. asiatica prefers cool regions at a relatively high elevation. These results can be used not only for identifying KBAs but also for the reference to a protection plan for H. asiatica in preparation of Korean reunification and climate change.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.85.2-85
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• 1994

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.94.2-95
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• 2000

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.93.2-93.2
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• 2000

• Horticultural Science & Technology
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• v.33 no.1
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• pp.24-30
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• 2015

This study was conducted to select proper light intensity, pot media, and fertilization level for potted Hepatica asiatica Nakai native to Korea. The plants were grown under various light intensities (shading rate, 52, 82, 90, 97%) imposed by shading net. Plants grown with 52% shading showed a low survival rate (65%). Survival rate increased as shading increased, with over 80% survival in shading above 90%. Growth indexes such as fresh weight and leaf number did not show any significant difference between shading treatments. Plants grown in a soil mixture of decomposed granite:fertilizer-amended media:Kanumatsuchi (60:10:30, v/v/v) or river sand:fertilizer-amended media:bark (50:20:30) showed over 85% survival. However, plants grown in a soil mixture of river sand:fertilizer-amended media:Kanumatsuchi (50:30:20) or upland:river sand (40:60) showed very low survival, below 60%. Leaf number and plant height were the highest in a soil mixture of decomposed granite:fertilizer-amended media:Kanumatsuchi (60:10:30) as well. To select a proper fertilization level for H. asiatica, hyponex solution diluted 1,000- or 2,000-fold were applied weekly or biweekly. The survival rate was lowest at weekly application with 1,000-fold diluted solution, and no significant difference was observed between other treatments. In conclusion, H. asiatica exhibits preferences for very low light intensity and soil with air permeability, and is adaptable to a broad range of fertilization levels.

• KSBB Journal
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• v.27 no.4
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• pp.227-231
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• 2012

Whole plants of Zostera asiatica were extracted twice with acetone/methylene chloride (A+M) and methanol (MeOH) in turn. The combined crude extracts were evaporated in vacuo and then the residue was partitioned between water and methylene chloride. The aqueous layer was fractionated into $H_2O$ and n-butanol and then the organic layer was also fractionated into 85% aq. MeOH and n-hexane, successively. The crude extracts and their solvent fractions were evaluated for their inhibitory effect on growth of human cancer cells AGS, HT-29, MCF-7, and HT-1080 cells by MTT reduction assay. Among samples tested, 85% aq. MeOH and n-hexane fractions showed strong cytotoxic effect against AGS, HT-29, and MCF-7 cells. On the other hand, for HT-1080 cell, 85% aq. MeOH fraction exhibited the strongest cytotoxic effect.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.156-164
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• 1987

Allelopathic activity of dominant upland weed species was determined using aqueous and methanol extracts and dried residues of the weeds. Germination and seedling growth of radish (Raphanus sativus L.) and sesame (Sesamum indicum L.) were greatly inhibited by 2% (w/v) aqueous extracts of Artemisia asiatica Nakai and Portulaca oleracea L., respectively. Great phytotoxicity on both indicator plants occurred by 2% (w/v) aqueous extracts of Capsel/a bursa-pastoris (L.) Medik. A complete inhibition in germination and seedling growth of radish was caused by 2% (w/v) methanol extract of A. asiatica. There was about 90% inhibition in germination and seedling growth of sesame due to 2% (w/v) methanol extracts of A. asiatica, P. oleracea and Trifolium repens L. Phytotoxic effects varied with extract solution, weed species and indicator plants employed, indicating that the weed species contained different contents and kinds of allelopathic chemicals. Water potential and pH adjusted to those of the extracts did not affect germination and seedling growth of the indicator plants. Phytotoxicity increased with increasing concentration of the extracts obtained from four suspected alleopathic weeds. Soil-incorporated plant residues of A. asiatica and P. oleracea caused greater phytotoxicity on radish than those of C. bursa-pastoris and T. repens.