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http://dx.doi.org/10.7841/ksbbj.2012.27.4.227

Cytotoxic Effect of Zostera asiatica on Growth of Human Cancer Cells  

Hong, Joo-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
Jung, Myung-Eun (Division of Marine Environment & Bioscience, Korea Maritime University)
Lee, Jung-Im (Division of Marine Environment & Bioscience, Korea Maritime University)
Kim, Ho-Jun (Division of Marine Environment & Bioscience, Korea Maritime University)
Chang, Jae-Soo (Department of Environmental Engineering, Korea Maritime University)
Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
KSBB Journal / v.27, no.4, 2012 , pp. 227-231 More about this Journal
Abstract
Whole plants of Zostera asiatica were extracted twice with acetone/methylene chloride (A+M) and methanol (MeOH) in turn. The combined crude extracts were evaporated in vacuo and then the residue was partitioned between water and methylene chloride. The aqueous layer was fractionated into $H_2O$ and n-butanol and then the organic layer was also fractionated into 85% aq. MeOH and n-hexane, successively. The crude extracts and their solvent fractions were evaluated for their inhibitory effect on growth of human cancer cells AGS, HT-29, MCF-7, and HT-1080 cells by MTT reduction assay. Among samples tested, 85% aq. MeOH and n-hexane fractions showed strong cytotoxic effect against AGS, HT-29, and MCF-7 cells. On the other hand, for HT-1080 cell, 85% aq. MeOH fraction exhibited the strongest cytotoxic effect.
Keywords
Zostera asiatica; human cancer cells; cytotoxic effect; MTT assay;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Roth, N. C. and A. M. Pregnall (1988) Nitrate reductase activity in Zostera marina. Mar. Biol. 99: 457-463.   DOI
2 Yang, L., J. Guoliang, and W. Zhiqiang (2010) Antibacterial activity of extracts from Zostera marina against pathogens of Apostichopus japonicus skin ulceration disease. J. Ocean Univ. Chin. 9: 68-70.   DOI
3 Choi, H. G., J. H. Lee, H. H. Park, and F. A. Q. Sayegh (2009) Antioxidant and antimicrobial activity of Zostera marina L. extract. Algae 24: 179-184.   과학기술학회마을   DOI
4 Kolenchenko, E. A., L. N. Sonina, and Y. S. Khotimchenko (2005) Comparative in vitro assessment of antioxidant activities of low-etherified pectin from the eelgrass Zostera marina and antioxidative medicines. Russian Journal of Marine Biology 31: 331-334.   DOI
5 Sonina, L. N. and M. Y. Khotimchenko (2007) Effectiveness of pectin extracted from the eelgrass Zostera marina for alleviating lead-induced liver injury. Russian Journal of Marine Biology 33: 204-206.   DOI
6 Hansen, M. B., S. E. Nielsen, and K. Berg (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods 119: 203-210.   DOI   ScienceOn
7 den Hartog, C. (1970) The Seagrass of the World. pp. 275. North-Holland Publishing Company, Amsterdam, Netherlands.
8 Shin, H. and H. K. Choi (1998) Taxonomy and distribution of Zostera (Zosteraceae) in eastern asia, with special reference to korea. Aquat. Bot. 60: 49-66.   DOI   ScienceOn
9 Cho, T. O. and S. M. Boo (1998) Marine flora of oeyondo islands on the yellow sea, korea: green algae and seagrass. Algae 13: 1-11.
10 Miki, S. (1932) On seagrass new to japan. Bot. Mag. 46: 774-788.   DOI
11 Miki, S. (1933) On the seagrass in Japan (I) Zostera and Phyllospadix, with special reference to morphological and ecological characters. Bot. Mag. 47: 842-862.   DOI
12 Lee, S. Y., C. J. Kwon, T. J. Kim, Y. B. Suh, and C. I. Choi (1999) Morphological examination of Zostera asiatica Miki (Zosteraceae) from various habitats. Korean J. Enviror. Biol. 17: 503-512.   과학기술학회마을
13 Khotimchenko, M. Y., K. V. Lenskaya, M. Y. Petrakova, Y. S. Khotimchenko, and V. V. Kovalev (2006) The mercury binding activity of pectin isolated from the seagrass Zostera marina. Russian Journal of Marine Biology 32: 312-215.   DOI
14 Kim, J. B., J. I. Park, and K. S. Lee (2009) Seagrass distribution in deukryang bay. Kor. J. Fish Aquat. Sci. 42: 509-517.   과학기술학회마을   DOI
15 Huh, S. H., S. N. Kwak, and K. W. Nam (1998) Seasonal variations of eelgrass (Zostera marina) and epiphytic algae in eelgrass beds in kwangyang bay. J. Kora Fish. Soc. 31: 56-62.   과학기술학회마을
16 Choi, T. S., K. Y. Kim, B. G. Lee, and J. S. Lee (2005) Preliminary study on the toxicity and transfer of heavy metals and tributyltin to seagrass Zostera marina. Algae 20: 157-166.   과학기술학회마을   DOI
17 Kim, J. B., J. I. Park, W. J. Choi, J. S. Lee, and K. S. Lee (2010) Spatial distribution and ecological characteristics of Zostera marina and Zostera japonica in the seomjin estuary. Kor. J. Fish Aquat. Sci. 43: 351-361.   과학기술학회마을   DOI   ScienceOn
18 Lee, S. H., S. M. Ma, Y. S. Lim, H. K. Choi, and H. C. Shin (2004) Genetic diversity and its implications in the conservation of endangered Zostera japonica in korea. J. Plant Biol. 47: 275-281.   과학기술학회마을   DOI
19 Lee, S. M., S. Y. Lee, and C. I. Choi (2005) Reproductive phenology of four korean seagrasses, Zostera caespitosa, Z. caulescens, Z. japonica and Z. marina. Ocean and Polar Research 27: 125-133.   DOI
20 Kumar, C. S., D. V. L. Sarada, T. P. Gideon, and R. Rengasamy (2008) Antibacterial activity of three south indian seagrasses. Cymodocea serrulata, Halophila ovalis and Zostera capensis. World J. Microbiol. Biotechnol. 24: 1989-1992.   DOI