• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.465-473
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• 2023

In this study, we aimed to evaluate the bioconversion ability of single (Lactiplantibacillus plantarum CBT LP3, Lactococcus lactis subsp. lactis CBT SL6, Streptococcus thermophilus CBT ST3) and multi-strain probiotics to convert rutin to quercetin in roasted tartary buckwheat, and to assess their biological activities. To evaluate the bioconversion efficiency, each strain was cultured for 24 h in MRS media with 5% roasted tartary buckwheat 'Hwangguem-Miso' powder. After then, rutin and quercetin contents were determined by HPLC. Additionally, the biological activities were compared before and after bioconversion of an ingredient. Anti-oxidant effects were measured by DPPH and ABTS assays. Anti-inflammatory effects were determined by measuring NO production, and levels of iNOS, TNF-α, IL-6 and IL-4 using an LPS-induced Raw 264.7 cell model. The bioconversion rate under the combination of three species of probiotics significantly increased more than single species. Antioxidant efficacy results showed the highest activity when the combination of three species of probiotics cultured. The pro-inflammatory factors such as nitric oxide, iNOS, TNF-a, and IL-6 were significantly decreased when the three types of probiotics were combined than single strain was cultured. In addition, level in the anti-inflammatory factor IL-4 was increased. The multi-strain probiotics showed increased bioconversion efficiency, effects of anti-oxidant and anti-inflammatory compared to the single strain. These findings suggest that the fermentation of tartary buckwheat by probiotics may be a valuable candidate for developing functional foods with anti-oxidation and anti-inflammation.

• Korean Journal of Food Science and Technology
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• v.42 no.1
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• pp.56-62
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• 2010

Quality characteristics such as alcohol, acid, and sugar contents of takju brewing mashes prepared using several yeasts were investigated during 12-days of fermentation. Among the yeasts examined, S. cerevisiae led to the highest level of ethanol (10.2-13.4%) and total minor alcohols (0.729-0.831 mg/mL). Regardless of the yeasts used, the acidity showed drastic changes (pH from 3.4-4.2 down to 2.4-2.7) during the first 2 days, and displayed negligible changes from day 4. The total acid contents rapidly increased to the first 4 days of fermentation and leveled off from 4 to the end of fermentation period. The common organic acid components were lactic, succinic and acetic acids. The total and reducing sugar contents varied depending on the yeasts used, with mashes prepared using S. coreanus and S. rouxii producing the maximum total sugar contents (5.43-5.5%) at the end of fermentation. The reducing sugar showed its maximum (7.53-14.89%) at day 2, after which it decreased to its minimum levels (3.04-4.52%). The common free sugar components were glucose and fructose, while S. ellipsoideus led to a higher free sugar level (0.35-5.29%).

• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.255-264
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• 2008

The effects of hot air dried tomato powder on the physicochemical and sensory properties of meat patties were studied. The control(C, no addition) and 4 treatments with addition of hot air dried tomato powder(T1, 0.25; T2, 0.50; T3, 0.75; and T4, 1.00%) were prepared and stored for 7 days at 5℃. The pH values of T4 were significantly lower(p<0.05) than those of control and other treatments during initial storage, however, the pH values of T4 were higher(p<0.05) at 7 days of storage. The cooking loss was not significantly different between control and all treatments. The 2-thiobarbituric reactive substances (TBARS) of meat patties containing hot air dried tomato powder were significantly lower(p<0.05) compared to those for control during the whole storage. The volatile basic nitrogen(VBN) values of T2 increased(p<0.05) significantly as the storage period increased, but there was no difference in VBN between control and the other treatments(T1, T3, T4). In meat color, L*, a* and b* of meat patties containing hot air dried tomato powder showed slightly higher (p>0.05) than that of control. a* and b* of T4 were the highest(p<0.05) among the all products. Total plate counts(TPC) increased(p<0.05) significantly as the storage period increased. The result of TPC showed the range of 5.48(T2)~6.98(C) log CFU/g at the 7 day of storage. Sensory panels evaluated that pork patties containing hot air dried tomato powder had the slightly higher score in overall acceptability.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.43 no.5 s.347
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• pp.7-16
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• 2006

IPTV is referred to the service which provide integrated IPTV services for providing video, 10/100-Mbit/sec Internet, voice, video-on-demand (VOD), and other broadband applications including home security, video conferencing, and telemedicine. All services are integrated into an IP (Internet Protocol) architecture designed specifically for Gigabit Ethernet FTTH systems, HFC or xDLC. It is absolutely necessary that telecon operators provide IP video delivery platforms that enable service providers to transform their business. With their own products, they can better manage their existing services and generate new revenues from broadcast TV, movies on demand and multimedia. Triple-play is a combination of broadcast, telephony and broadband services offered through IPTV networks. With cable operators allowed to offer a triple-play bundle, the nation's telecom operators are beginning to get a little anxious. Cable operators assert that triple-play is a must-have and natural extension of the cable service bundle. The Korean Cable TV Association asserts that the triple-play model is of paramount importance to the cable industry's future growth. But the telecom sector considers itself unfairly disadvantaged, saying they cannot compete until regulatory issues are resolved. The start of web-based television in Korea may still be some time off with a confrontation between the nation's IT regulator and broadcasting sector over the service's legal boundaries shows no signs of being resolved my time soon. korea should be is the fastest-growing provider of IPTV solutions in the industry, with over worldwide customers.

• BMB Reports
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• v.40 no.2
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• pp.189-195
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• 2007

Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.

• Journal of Broadcast Engineering
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• v.14 no.5
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• pp.573-588
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• 2009

In this paper, we propose a novel video quality degradation monitoring scheme titled VR-VQMS(Visual Rhythm based Video Quality Monitoring Scheme) over an IPTV service prone to packet losses during network transmission. Proposed scheme quantifies the amount of quality degradation due to packet losses, and can be classified into a RR(reduced-reference) based quality measurement scheme exploiting visual rhythm data of H.264-encoded video frames at a media server and reconstructed ones at an Set-top Box as feature information. Two scenarios, On-line and Off-line VR-VQMS, are proposed as the practical solutions. We define the NPSNR(Networked Peak-to-peak Signal-to-Noise Ratio) modified by the well-known PSNR as a new objective quality metric, and several additional objective and subjective metrics based on it to obtain the statistics on timing, duration, occurrence, and amount of quality degradation. Simulation results show that the proposed method closely approximates the results from 2D video frames and gives good estimation of subjective quality(i.e.,MOS(mean opinion score)) performed by 10 test observers. We expect that the proposed scheme can play a role as a practical solution to monitor the video quality experienced by individual customers in a commercial IPTV service, and be implemented as a small and light agent program running on a resource-limited set-top box.

• Journal of Broadcast Engineering
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• v.12 no.4
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• pp.360-372
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• 2007

We propose a fast inter-layer mode decision method by utilizing coding information of base layer upward its enhancement layer inscalable video coding (SVC), also called MPEG-4 part 10 Advanced Video Coding Amendment 3 or H.264 Scalable Extension (SE) which is being standardized. In this paper, when the motion vectors from the base layer have zero motion (0, 0) in inter-layer motion prediction or the Integer Transform coefficients of the residual between current MB and the motion compensated MB by the predicted motion vectors from the base layer are all zero, the block mode of the corresponding block to be encoded at the enhancement layer is determined to be the $16{\times}16$ mode. In addition, if the predicted mode of the MB to be encoded at the enhancement layer is not equal to the $16{\times}16$ mode, then the rate-distortion optimization is only performed on the reduced candidated modes which are same or smaller partitioned modes. Our proposed method exhibits the complexity reduction in encoding time up to 72%. Nevertheless, it shows negligible PSNR degradation and bit rate increase up to 0.25dB and 1.73%, respectively.

• Korean Journal of Pharmacognosy
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• v.41 no.4
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• pp.264-269
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• 2010

In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction ($3.68\;{\mu}g/mL$) and aglycone fraction ($3.15\;{\mu}g/mL$) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) of Euphorbia humifusa extract on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($0.43\;{\mu}g/mL$) and aglycone fraction ($0.35\;{\mu}g/mL$) of extract showed higher ROS scavenging activity than L-ascorbic acid ($1.50\;{\mu}g/mL$). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner ($5{\sim}25\;{\mu}g/mL$), and was more effective than (+)-${\alpha}$-tocopherol, lipid peroxidation chain blocker. Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.

• Korean Journal of Applied Biomechanics
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• v.13 no.3
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• pp.253-264
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• 2003

This study aims at finding the structure of spike technique by analysing comparatively the spike action by excellent and by non-excellent players throughout the section from a flying jump to the time of landing for the correct analysis of spike action and tries to help athletes and coaches to execute a scientific training. For the objected person of this study, six of H College athletes three of excellent athletes and three of non-excellent athletes, presently registered as athlete with the Korea Volleyball federation) were chosen, and the factors of analysis were analysed upon performance time of action by section, human body centered displacement, change of articulation angle, speed change of articulation of the upper limbs, uniformity of the articulation of the upper limbs upon impact, etc. The conclusion of this study is as follow: 1. In the time required for taking action, it shows to take $1.067{\pm}0.057$ seconds for the group of excellent athletes and $1.034{\pm}0.033$ seconds for the group of non-excellent athletes. Although there was not big difference between two groups in the performance time of action, it showed that the group of excellent athletes takes longer compared to the group of non-excellent athletes. And it was found by the result of this study that the group of excellent athletes stays longer in the duration of flight. 2. In the displacements of horizontal movement and vertical movement, it was found that the group of excellent athletes have moved more than the group of non-excellent athletes in the horizontal movement of the center of human body 3. In the angles of wrist and knee, it was found that the excellent athletes have shown little than the non-excellent athletes in the entire sections, but that in the angle of elbow, the non-excellent athletes have shown bigger than the excellent athletes.. 4. In the speed of the articulation of the upper limbs upon impact, it was found that the group of excellent athletes have shown bigger than the group of non-excellent athletes, and that in the maximum value of the articulation of the upper limbs, the maximum value for the hand was indicated upon impact and that forearm and upper arm have shown the maximum value just before the impact. 5. In the uniformity of articulation of the upper limbs at the time of impact, the group of excellent athletes showed bigger than the group of non-excellent athletes in all the articulations.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.223-227
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• 2010

These study was carried out to investigate the effects of the recovery time, diameter of oocytes on in vitro fertilization or intracytoplasmic sperm injection (ICSI). The in vitro maturation rates to MII stage of oocytes recovered at the inactive, follicular and luteal stages matured for 72 h were $1.4{\pm}0.0%$, $43.4{\pm}3.2%$ and $10.8{\pm}2.7%$, respectively. The fertilization rates of in vitro cultured oocytes recovered from ovaries at the in active, follicular and luteal stages were $0.0{\pm}0.0%$, $15.7{\pm}3.4%$ and $7.6{\pm}3.5%$, respectively. The in vitro maturation rate of oocytes recovered from ovaries at the follicular stage of the reproductive cycle was significantly higher than those at the inactive and luteal stages (p<0.05). The penetration rate determined that the percentages of oocytes with diameters in the < $100\;{\mu}m$, 100 to $100\;{\mu}m$ and 110 to $120\;{\mu}m$ ranges were $17.5{\pm}4.7%$, $43.9{\pm}4.5%$, $21.3{\pm}3.4%$, respectively. The penetration rate of oocytes with diameters between 100 to $100\;{\mu}m$ was significantly higher than that of oocytes whose diameters were < $100\;{\mu}m$ and $110{\sim}120\;{\mu}m$ (p<0.05). The penetration rate of oocytes determined that the percentages of ovaries with diameters between 1 to 5 mm and 6 to 10 mm were $32.9{\pm}3.2%$ and $17.5{\pm}3.7%$, respectively. Thus, the diameters of the ovaries were significantly higher at 1 to 5 mm (p<0.05). A total of 264 oocytes were fixed and stained after co-incubation with sperm, of which 72 had identifiable nuclear material. After in vitro fertilization for 20 hrs, 27.3% of oocytes were penetrated by spermatozoas. Oocytes were fixed and stained after ICSI, of which 38 oocytes contained identifiable nuclear material. After in vitro fertilization and ICSI for 20 hrs, to 27.3% and 67.9% of oocytes were penetrated by spermatozoas. The in vitro fertilization rates by ICSI was significantly higher than that in vitro fertilization method (p<0.05).