Browse > Article

Cellular Protective Effect and Component Analysis of Euphorbia humifusa Extracts  

Kim, Sun-Young (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
Won, Doo-Hyun (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
Lim, Myoung-Sun (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
Park, Soo-Nam (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
Publication Information
Korean Journal of Pharmacognosy / v.41, no.4, 2010 , pp. 264-269 More about this Journal
Abstract
In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction ($3.68\;{\mu}g/mL$) and aglycone fraction ($3.15\;{\mu}g/mL$) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) of Euphorbia humifusa extract on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction ($0.43\;{\mu}g/mL$) and aglycone fraction ($0.35\;{\mu}g/mL$) of extract showed higher ROS scavenging activity than L-ascorbic acid ($1.50\;{\mu}g/mL$). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner ($5{\sim}25\;{\mu}g/mL$), and was more effective than (+)-${\alpha}$-tocopherol, lipid peroxidation chain blocker. Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.
Keywords
Euphorbia humifusa; Cellular protective effect; Antioxidant; Quercetin; Kaempferol;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Darr, D. and Fridovich, I. (1994) Free radicals in cutameous biology. F. Invest. Dermatol. 102: 671-675.   DOI   ScienceOn
2 Eeo, S. I., Hu, W., Han, W. and Wang, M. H. (2008) Antioxidant activity and cytotoxic effect of extracts from Euphorbia humifusa. Kor. J. Phamacogn. 39: 295-299.   과학기술학회마을
3 Cha, B. C. Lee, S. K. Lee, H. W. E. Choi, M. Y. Rhim, T. J. and Park, H. J. (1997) Antioxidative effect of domestic plants. Kor. J. Pharmacogn. 28(1): 15-20
4 McCord, J. M. and Fridovich, I. (1969) Superoxide dismutase an enzymic function for erythrocuprein. J. Biol. Chem. 244: 6049-6103.
5 배기환 (2000) 한국의 약용식물, 277. 교학사, 서울.
6 Jurkeiwicz, B. A., Bissett, D. L. and Buettner, G. R. (1995) Effect of topically applied tocopherol on ultraviolet radiationmediated free radical demage in skin. J. Invest. Dermatol. 104: 484-488.   DOI   ScienceOn
7 Fantone, J. C. and Ward, P. A. (1982) Role of oxygenderived free radicals and metabolites in leukocyte dependent inflammatory reaction. Ann. J. Path. 107(3): 395-418.
8 Jurkiewicz, B. A. and Buettner, G. R. (1994) Ultraviolet lightinduced free radial formation in skin : An electron paramagnetic resonance study. Photochem. Photobiol. 59(1): 1-4.
9 Jurkiewicz, B. A. and Buettner, G. R. (1994) Ultraviolet lightinduced free radical formation in skin: An electron paramagnetic resonance study. Photochem. Photobiol. 59: 1-4.   DOI   ScienceOn
10 Packer, L. (1994) Ultraviolet radiation (UVA, UVB) and skin antioxidants. In: Free radical damage and its control. Rice-Evans, C. A. and Burdon, R. H. (eds.). 239-253. Elsevier Science, St. Louis.
11 Thomas, J. P. and Girotti, A. W. (1988) Photogenenration of singlet oxygen by membrane bound hematoporphyrin derivative. Photochem. Photobiol. 47: 79s.
12 Zheng, P. and Kligman, L. H. (1992) UVA induced biochemical changes in hairless mouse skin : A comparison to UVB-Induces Damage. J. Invest. Dermatol. 100: 194-199
13 Foote, C. S. (1991) Definition of type I and type II photosensitized oxidation. Photochem. Photobiol. 54: 659-666.   DOI   ScienceOn
14 Scharffetter-Kochanek, K. (1997) Photoaging of the connective tissue of skin: Its prevention and therapy, antioxidants in disease mechanism and therapy. Adv. Pharmacol. 38: 639-655.
15 Cheseman, K. H. and Slater, T. F. (1993) Free radicals inmedicine. British Medical Bulletin 49(3): 566-576.
16 Park, S. N. (1989) Dissertation, Seoul National Univ., Seoul, Korea.
17 Davies, K. J. A. (1987) Protein damage and degradation by oxygen radical. J. biol. Chem. 262(20): 9902-9907.