• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.524-524
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• 2023

하천 내 식생의 분포는 흐름저항의 증가와 수위상승에 큰 영향을 미치는 요소 중 하나이다. 동일한 단면정보를 가졌더라도 식생이 분포하는 하도는 식생이 없는 하도에 비해 흐름저항으로 인해 유속이 현저히 느려져 홍수위 상승을 유발하기 때문이다. 따라서 식생의 종류, 크기, 분포 형태, 잎의 밀도 등에 따라 흐름저항계수를 정량화하며 흐름을 정확히 예측하는 것이 필요하다. 본 연구에서는 2019년 한국건설기술연구원 하천실험센터에서 진행된 식생하도에 대한 실규모 실험의 조건과 지형정보를 HEC-RAS(Hydrologic Engineering Center's River Analysis System) 1차원 수치모형에 입력하고, 식생 패치의 분포를 고려한 Manning's n의 공간적 분포 및 적용방식에 따른 수면 경사 재현 정확도에 대한 민감도 분석을 수행하였다. 실험은 상단 폭 11 m, 경사 1:2(V:H)의 사다리꼴 단면을 가진 실규모 수로에서 70 m 길이의 구간을 대상으로 진행되었다. 실험 구간 내 6개의 압력식 수위계를 설치해 수위 측정 및 수면 경사 산정을 실행하였다. 실험 조건으로 적용된 인공 식생패치의 분포 및 밀도 조건은 3가지로 큰 패치와 작은 패치로 구성된 조밀한 조건, 단일 패치로 구성된 조밀한 조건, 단일 패치로 구성된 성긴 조건이었으며, 모두 정수(emergent)상태로 진행되었다. 적용된 패치의 형상은 내성천에서 조사된 자연 형태의 식생패치 형태를 참고하였으며, 버드나무 종을 모사하였다. 실험 조건에 따라 유량은 각각 평균 1.5 cms와 2.7 cms로 공급하였으며, 평균 수심은 약 1 m로 측정되었다. 위 실험 내용을 바탕으로 수치모의를 위한 경계조건과 지형정보를 수립하였으며 모의 케이스는 크게 두 가지로, 수로 내 식생의 분포를 종방향으로 고려한 케이스와 횡방향으로 고려하여 조도계수를 적용한 케이스로 분류하였다. 모의에 적용된 조도계수는 실험에서 획득한 데이터와 베르누이 방정식을 활용하여 산정되었으며, 두 케이스에 대한 모의 결과는 실험에서 관측된 수위와 비교하였다. 본 연구에 따르면 여러 개의 식생패치가 정수상태로 존재하는 하천에 대한 1차원 수치모의 시 식생의 분포를 종방향으로 고려하여 하나의 구간조도계수를 적용하는 방식이 종횡단면의 식생패치 위치를 고려한 조도계수를 세분화하여 적용하는 방식에 비해 수위 계산 정확도가 상대적으로 높은 것으로 나타났다.

• Proceedings of the Korean Society of Toxicology Conference
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• 1998.10a
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• pp.36-36
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• 1998

Tumorigenicity of benzo(a)pyrene (BP) and benzo(a)pyrene diol epoxides ((+)BPDE-1, (-)BPDE-1) was investigated in transgenic TG-AC mice carrying v-Ha-ras oncogene fused to the promoter of the mouse embryonic a-like, z-globin gene. Animals were topically treated twice per week for 25weeks with BPDE (10$\mu$g/mouse) and BP (10, 20, 40$\mu$g/mouse). In addition, animals were treated with BPDE or BP (initiated) followed by TPA (2$\times$2.5$\mu$g/week, for 4 weeks) for promotion study. In the continuous treatment of BPDE or BP, animals treated with 40$\mu$g BP showed $100\%$ tumor response after 20 weeks, $40\%$ of mice for 20$\mu$g BP, and $20\%$ for (+)BPDE-1, but (-)BPDE-1 and 10$\mu$g BP did not show any tumor response. After 25 weeks, most tumors turned out to be carcinomas in animals treated with 40$\mu$g BP. In BPDE or BP/TPA Initiation-promotion study, papilloma response occurred earlier (6 weeks after TPA treatment) than in continuously treated animals with BPDE or BP. RT-PCR assay for transgene expression showed that BP or BPOE was not transgene dependent in its tumorigenicity, but TPA was. Several Cytokine genes(TGF-a, TNF-a) and c-myc gene expressions were monitored in skin tissues during BP carcinogenesis. In early stage of BP treatment, the gene expressions were elevated(c-myc,TGF-a) or unchanged(TNF-a) compared to control, but the levels were gradually decreased during both middle and late stages of cacinogenesis, Gene expression levels of skin papillomas in acetone initiated-TPA promoted animals were close to those of middle stage or between middle and late stages. i-NOS was also highly expressed in carcinoma and papilloma, These data suggest that transgene expressions of TG-AC mice were not dependent on BP carcinogenesis and that TG-AC mice were more sensitive to TPA regardless of types of initiators. In addition, genes(TGF-a, c-myc, TNF-a, i-NOS) were modulated in the skin during BP cacinogenesis or TPA promotion.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.180-180
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• 1994

Farnesyl protein transferase는 Ras precursor의 C-terminal에 있는 cystein residue에 farnesyl group을 결합시키는 효소다. 이 효소를 bovine testis에서 30-50% ammonium sulfate fractionation, DEAE-sephacel ion exchange, Sephacryl s-300 gel filtration, hexapeptide(KKCVIM) affinity chromatography를 통해 30000배로 분리하였다. 분리된 효소는 gel filtration시 약 100kDa으로, SDS-polyacrylamide 전기영동시 50kDa의 인접한 두 bands로 나타났고 이것은 $\alpha$, $\beta$ subunits으로 생각되었다. $\alpha$ subunit을 encoding하는 RAM2 유전자를 site directed mutagenesis로 145번의 histidine을 aspartate로, 140번의 aspartate를 asparagine 으로 바꾸었더니 optimal pH와 $K_{m}$ 값이 변했다. Diethyl pyrocarbonate로 histidine residues를 chemical modification시켰을때 효소의 활성이 저하되었다. 145번 histidine이 aspartate로 바뀐 돌연변이효소에서 비교적 느리게 활성이 저하되므로 145번 histidine이 이 효소의 active site에 있을것으로 추측된다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.55-55
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• 1993

Farnesyl Protein transferase(FPT)는 발암유전자 ras의 단백질 산물인 p$^{21}$의 post-translational modification의 첫 단계인 ras-farnesylation에 관여하는 효소로 본 연구에서는 정제된 FPT와 E. coli에서의 발현 system을 이용하여 FPT의 구조와 기능을 밝히고 이를 FPT 방해제의 설계에 이용하고자 한다. Bovine testis에 존재하는 FPT를 30%-50%의 Ammonium sulfate로 fractionation하고, DEAE-Sephacel, Sephacryl S-300 column을 통과시킨 후 peptide(KKCVIM) affinity column을 이용하여 순수 정제하였다. 정제된 효소의 분자량은 gel-filtration에 의해 100KDa으로 추정되었고 SDS-PAGE 결과 49KDa과 46KDa의 두 subunit로 구성되었음이 확인되었다. 효소활성에는 $Mg^{2＋}$ 와 $Zn^{2＋}$가 필수적이며 최적 pH는 7.0이었다. Yeast의 FPT의 두 subunit 유전자는 Yeast genomic DNA를 template로 사용하고 각 subunit에 specific한 합성된 primer들과 vent polymerase를 이용하여 Polymerase chain reaction을 통하여 얻었다. 두 유전자를 pBluescriptII SK＋ vector를 변형시킨 두 vector, pBSK＋4와 pBChl＋4에 재조합 시킨 후 E.coli에 transformation시켜 발현시켰다. 현재 정제된 Bovine FPT와 E. coli에서 발현된 Yeast FPT의 chemical modification과 site-directed mutagenesis를 통하여 FPT의 active site와 substrate binding site에 관한 연구를 진행시키고 있다.

• Proceedings of the KIEE Conference
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• 2000.07d
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• pp.3184-3186
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• 2000

This paper describes the remote monitoring & diagnosis system of tower parking facilities. This system consists of central station, monitoring equipments and parking system control panel. The central station is developed under client/server architecture, and the monitoring systems are connected to central station by LAN using RAS constructed PSTN. This system offers real-time fault detection and data acquisition of tower parking system.

• Proceedings of the KIEE Conference
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• 1999.07g
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• pp.3206-3208
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• 1999

This paper describes the remote fault monitoring system for tower parking facilities. This system consists of central station, remote monitoring equipments and communication equipments. The central station is developed under client-server architecture which composed a DB server, a fault detection client, a status collection client and a A/S client. And the remote monitoring systems are connected to central station by LAN using RAS(Remote Access Service) which is constructed PSTN(Public Switched Telephone Network). This system offers real-time fault detection and status data acquisition of tower parking system.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2000.04a
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• pp.91-98
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• 2000

Derivation of stage-discharge relationship and characteristics for Yangsan river is presented in this paper. This research has been conducted as the second one after the first trial of 1997. The determination of discharge at a Yangsan river gauging was best made by measuring the flow velocities with a current meter and rod float. The rating curve obtained through 52 stage-discharge measurements on Yangsan river basin in 1999 is represented by Q=15.3540-140.6076H+182.44372$H^2$, which is discovered to be most excellent among other curves in reliability analysis. The capability of the observed stage-discharge data for Yangsan river was tested by HEC-RAS program, and its capability to reproduce discharge was investigated and compared with the computational results. Rating curve stability is determined on the basis of deviations in the stage-discharge relationship, utilization of specific gauge, and absolute differences between sequential stream flow measurements and an analysis residuals. Therefore it seems necessary to research method to obtain rating curve in a rigorous and accurate manner.

• BMB Reports
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• v.39 no.1
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• pp.1-8
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• 2006

Helicobacter pylori is the definitive carcinogen for stomach cancer and is known to induce proinflammatory cytokines, such as tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin-1(IL-1) in the stomach. Based on our findings that TNF-$\alpha$ is an endogenous tumor promoter, we identified the TNF-$\alpha$ inducing protein (Tip$\alpha$) gene family, and confirmed Tip$\alpha$ and HP-MP1 as new carcinogenic proteins of H. pylori. Tip$\alpha$ protein is unique to H. pylori, and this paper shows the strong tumor promoting activity of Tip$\alpha$ gene family, in cooperation with Ras protein and its mechanisms of action in relation to NF-${\kappa}B$ activation, and discusses the carcinogenic role of Tip$\alpha$ in stomach cancer. Our recent finding showing that penicillin-binding proteins of other bacteria are weak homologues of Tip$\alpha$ is also discussed.

• Molecules and Cells
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• v.20 no.2
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• pp.280-287
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• 2005

The insulin-mediated Ras/mitogen-activated protein (MAP) kinase cascade was examined in SK-N-BE(2) and PC12 cells, which can and cannot produce reactive oxygen species (ROS), respectively. Tyrosine phosphorylation of the insulin receptor and insulin receptor substrate 1 (IRS-1) was much lower in SK-N-BE(2) cells than in PC12 cells when the cells were treated with insulin. The insulin-mediated interaction of IRS-1 with Grb2 was observed in PC12 but not in SK-N-BE(2) cells. Moreover, the activity of extracellular-signal-related kinase (ERK) was much lower in SK-N-BE(2) than in PC12 cells when the cells were treated with insulin. Application of exogenous $H_2O_2$ caused increased tyrosine phosphorylation and Grb2 binding to IRS-1 in SK-N-BE(2) cells, while exposure to an $H_2O_2$ scavenger (N-acetylcysteine) or to a phophatidylinositol-3 kinase inhibitor (wortmannin), and expression of a dominant negative Rac1, decreased the activation of ERK in insulin-stimulated PC12 cells. These results indicate that the transient increase of ROS is needed to activate ERK in insulin-mediated signaling and that an inability to generate ROS is the reason for the insulin insensitivity of SK-N-BE(2) cells.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.120-123
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• 2009

The WiBro to start June 2006 is mobile internet service with 60km/h, 3Mbps. And current WiBro service is used in Seoul, the metropolitan area and the local major cities. This study suggests WiBro technology and analyzes wireless communication technology to vehicle-to-infrastructure communications for the unmanned vehicle control. We were driving vehicle with two laptop computer's WiBro unit for V2I communication environment. So each RAS(Radio Access Station) on center is tested coverage area, transmission rate, latency rate, lost rate and transmission rate to parking area environment.