• Proceedings of the IEEK Conference
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• summer
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• pp.77-81
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• 2004

In this paper, we propose a analog front-end (AFE) for noncoherent On-Off Keying (OOK) Ultra Wide Band (UWB) system based on power detection. The proposed AFE are designed using 0.18 micron CMOS technology and verified by simulation using SPICE. The proposed AFE consist of Sample-and-Hold block, Analog-to-Digital converter, synchronizer, delayed clock generator and impulse generator. The time resolution of 1ns is obtained with 100MHz system clocks and the synchronized 10-bit digital outputs are delivered to the baseband. The impulse generator produces 1ns width pulse using digital CMOS gates. The simulation results show the feasibility of the proposed UWB AFE systems.

• 전자공학회논문지 IE
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• v.45 no.4
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• pp.1-6
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• 2008

A fast-hopping frequency synthesizer that reduces complexity and power consumption is presented for MB-OFDM UWB applications. The proposed architecture uses 3960 MHz LC VCO, 528 MHz ring oscillator, passive mixer and LC-tuned Q-enhancement BPF to generate Band Group 1 frequencies. The adjacent channel rejection ratio is less than -40 dBc for 3432 MHz and -H dBc for 4488 MHz. A fast switching SCL-tpre MUX is used to produce the required channel output signal and it takes less than 2.2 ns for band switching. The total power consumption is 47.9 mW from a 1.8 V supply.

• BMB Reports
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• v.32 no.5
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• pp.474-479
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• 1999

Using a hybridoma technique, spleen cells of Balb/c mice immunized with human chorionic gonadotropin (hCG) were fused with NS-1 mouse myeloma cells. Two hybrid cell lines, clones KS-8 and KS-19, secreting monoclonal antibodies to hCG, were isolated. KS-8 and KS-19 belong to the immunoglobulin $G_1$ subclass. With the aid of a double-antibody radioimmunoassay, it was established that the KS-8 monoclonal antibody recognizes an immunodeterminant of the $\beta$-subunit of hCG, whereas the KS-19 monoclonal antibody recognizes an epitope present on the $\alpha$-subunit of hCG. The KS-8 monoclonal antibody specifically reacts with human chorionic gonadotropin and shows cross-reactivity of less than 0.3% to other related human glycoprotein hormones. On the other hand, using a hemagglutination test based on antibody-induced agglutination of sheep red blood cells coated with hCG, It was shown that only the KS-19 monoclonal antibody was capable of inducing a positive reaction, although both monoclonal antibodies had similar binding capacity to the coated cells. The results from the dual screening procedures demonstrate that KS-8 and KS-19 monoclonal antibodies show high sensitivity in two different assays, and are hence useful for the qualitative and quantitative determination of hCG by both radioimmunoassay and hemagglutination inhibition tests.

• BMB Reports
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• v.45 no.11
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• pp.653-658
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• 2012

After the outbreak of the swine-origin influenza A H1N1 virus in April 2009, World Health Organization declared this novel H1N1 virus as the first pandemic influenza virus (2009 pH1N1) of the $21^{st}$ century. To elucidate the characteristics of 2009 pH1N1, the growth properties of A/Korea/01/09 (K/09) was analyzed in cells. Interestingly, the maximal titer of K/09 was higher than that of a seasonal H1N1 virus isolated in Korea 2008 (S/08) though the RNP complex of K/09 was less competent than that of S/08. In addition, the NS1 protein of K/09 was determined as a weak interferon antagonist as compared to that of S/08. Thus, in order to confine genetic determinants of K/09, activities of two major surface glycoproteins were analyzed. Interestingly, K/09 possesses highly reactive NA proteins and weak HA cell-binding avidity. These findings suggest that the surface glycoproteins might be a key factor in the features of 2009 pH1N1.

• Korean Journal of Veterinary Service
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• v.40 no.3
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• pp.187-192
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• 2017

In this report, fifteen monoclonal antibodies (MAbs) against an avian influenza virus (H9N2 subtype) were newly produced and characterized. These MAbs proved to react to the epitopes of nucleocapsid protein (NP), hemagglutinin (HA), neuraminidase (NA) and non-structural protein 1 (NS1) of Korean H9N2 strain, respectively. Two HA-specific MAbs showed the ability to inhibit the hemagglutination activity of H9N2 subtype avian influenza virus when tested by hemagglutination inhibition (HI) assay. All MAbs did not cross-react with other avian-origin viruses (Newcastle disease virus, infectious bursal disease virus, infectious bronchitis virus and avian rotavirus) by immunofluorescence test or enzyme-linked immunosorbent assay. The MAbs produced in this study could be useful as the materials for diagnostics and therapeutics against Korean-lineage H9N2 virus infections.

• Journal of Microbiology and Biotechnology
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• v.4 no.2
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• pp.113-118
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• 1994

A bacterial strain NS-83 isolated from soil was able to produce an extracellular thermostable protease. The strain was identified as Pseudomonas aeruginosa based on its morphological and physiological characteristics. A thermostable protease from this strain has been purified to homogeneity as judged by SDS-PAGE and isoelectric focusing. The purification procedures included hydrophobic interaction, ion exchange, and gel filtration chromatography. The $M_r$ and the pl of the enzyme were 32,000 and 5.9, respectively. The optimal pH at 55$^{\circ}C$ and the optimal temperature at pH 7.0 were 8.0 and 60$^{\circ}C$, respectively. The D-values of the enzyme at 60, 65, and 70$^{\circ}C$ were 22, 2.1, and 0.75 hrs, respectively. The enzyme activity was significantly inhibited in the presence of 1 mM o-phenanthroline or EDTA, suggesting that the enzyme is metalloprotease. The $K_m$, and $V_{max}$ for Hammarsten casein were found to be 3.2 mg/ml and 0.918 unit/ml, respectively. These enzymatic properties were similar to those of elastase produced from P. aeruginosa IFO 3455, but the enzyme was clearly different from the reported elastase, in respect to $Ca^{++}$ effects on enzyme-thermostability. This property, together with amino acid composition analysis, confirmed that the enzyme differs from the known P. aeruginosa elastase.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.2
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• pp.41-47
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• 2008

Most of media compression CODECs adopts the variable length coding method. This paper proposes special registers and instruction set for bit stream process in order to accelerate the decoding process of the variable length code. The instruction set shares the conventional data path to minimize additional costs. And bit stream is read from the memory instead of the special port. Therefore the instruction set minimizes the change of the processor, and is adopted without any additional input controller and buffer, and accelerate decoding process of variable length code. The data path of the instruction set needs additional 65 bits memory and 344 equivalent gates, 0.19 ns delay under TSMC $0.25{\mu}m$ technology. The instruction set reduced the execution time of the variable length code decoding process in H.264/AVC by about 55%.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.420-426
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• 1998

An extracellular glutamyl aminopeptidase (EC 3.4.11.7) producing bacterium was isolated from soil and identified as Bacillus licheniformis based on its morphological and physiological characteristics. The aminopeptidase was purified to homogeneity by ammonium sulfate precipitation, Phenyl Sepharose, Resource Q, and Superose 12 column chromatographies. The specific activity of the purified aminopeptidase was 9.2 unit/mg for glutamyl p-nitroanilide with 17.6 purification folds. The purified aminopeptidase had an estimated molecular mass of 64 kDa consists of two different subunits (42 kDa and 22 kDa), and its isoeletric point was 5.2 measured by isoelectric focusing. The optimum pH and temperature of the aminopeptidase were 8.0 and 55$^{\circ}C$, respectively. The aminopeptidase was inhibited by EDTA and 1,10-phenanthroline, suggesting it be a metalloenzyme. Comparing with other aminopeptidase, the enzyme showed relatively high activity against peptide having glutamic acid as N-terminal.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.10 no.2
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• pp.1-6
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• 2010

Short-range wireless communication systems are expanding at rapid rate, finding application in offices and homes. Development of wireless local network is accompanied by steady increase in the demand for ever higher data rates. This in turn entails the necessity to develop communication systems which operate at higher frequencies. It can be expected that short-rage wireless communication networks will soon push towards the THz frequency range. We use a 3D ray-launching for analysis of propagation environments at the indoor fixtures. We extended the approach from the modeling of the reflectivity of optically thick, smooth building materials at THz frequencies to materials with a rough surface. The simulation result of propagation environment is similar to average received power of reference paper. The RMS delay spread was calculated to be 9.11 ns in a room size of $6m(L){\times}5m(W){\times}2.5m(H)$ for the concrete plaster.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.4
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• pp.745-752
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• 2005

In this paper we propose and implement a digital part of a receiver system for identifying a moving object and its tracking position in wireless environment. We assumed UWB(Ultra Wide Band)-based communication system for target application and used serial communication method(RS-232). The proposed digital receiver consists of RS-232-type1/RS-232-type2 for input and output of serial communication, ID Detector for detecting IDs, and PISO&Buffer circuit to buffer input signals for appropriate operation of ID Detector. We implemented the digital receiver with minimal hardware(H/W) resource according to target application of UWB-based communication system. So it correlates input patterns with pre-stored patterns though repeated detecting method for multiple IDs. Since it has reference panerns in the Ve-stored form, it can detect various IDs instantly. Also we can program content and size of reference patterns considering compatibility with other systems .The implemented H/W was mapped into XC2S100PQ208-5 FPGA of Xilinx, occupied 727($30\%$) cells, and stably operated in the clock frequency of 75MHz(13.341ns).