• Title/Summary/Keyword: H type

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Cation Flux-Mediated Activation of P-Type ATPase in Helicobacter pylori

  • Yun, Soon-Kyu;Ki, Mi-Ran;Park, Jeong-Kyu;Lim, Wang-Jin;Hwang, Se-Young
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.441-448
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    • 2000
  • The production and cation flux-mediated activation of the P-type ATPase in Helicobacter pylori was investigated. Using the polymerase chain reaction (PCR), the proton pump genotype of H. pylori was found to be positive for both F-type and P-type ATPases. Yet, their production in terms of enzyme specific activity varied substantially depending on H. pylori strains, ranging over 3-fold. Its main constituent appeared to be the P-type ATPase pool, in contrast to other common bacterial compositions. Interestingly, the F-type ATPase was observed only when intact H. pyloricells were exposed to pH 4.5 or above (37$^{\circ}C$ for 1 h). In contrast, significant amounts of the P-type ATPase still remained after 1 h of cell treatment even at pH below 4.5. By enriching the acidic medium with RPMI(pH 3.0), the P-type ATPase was stabilized, accompained by inactivation of the F-type ATPase. Using H. pylori membrane vesicles, it was found that ammionia-mediated cation flux increased the rate of ATP hydrolysis by the P-type ATPase. Accordingly, these data strongly suggest that the P-type ATPase is involved or functions as an effective regulator for the cation flux across the H. pylori membrane, thereby reducing the risk of excess proton influx.

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Classification of Body Types for Pattern Grading of Ready-to-Wear -focusing on Korean Males aged from 44 to 54- (신사복의 패턴 그레이딩을 위한 체형 분류 -44세에서 54세사이의 한국 성인 남성을 대상으로-)

  • 김구자;정명숙
    • Journal of the Korean Society of Clothing and Textiles
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    • v.25 no.6
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    • pp.1069-1078
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    • 2001
  • Pattern grading is a technique used to increase or decrease the size of a garment pattern according to the measurements in a given size chart. The original pattern is graded and laid out for cutting before mass production. This study tried to classify body types for pattern grading of jacket by applying a concept of "drop"defined as the difference between chest girth and waist girth and the difference between hip girth and waist girth for pants. Data were collected through the stratified sampling method. 138 subjects were selected out of 1,290 subjects of our sample population. Findings were as follows : 1) For pattern grading of jacket, the cell with the chest girth of 96cm and the waist girth of 87cm had the highest frequency rate and body type was 87H type and the coverage of this type was 9.52%. Then, the size specification 87-96 was the center of distribution. H type had seven ones such as 72H, 75H, 78H, 81H, 84H. 87H and 90H. H type had 33 observations and frequency ratio of 26.19%. Same types could be graded up and down from the reference size for the age group. And this reference size became to the starting point for developing the grading system. 2) For pattern grading of pants, fatty types, H10 type had six ones such as 80H10. 82H10, 84H10, 86H10, 88H10 and 90H10. H10 type had 28 observations and frequency ratio of 20.29%. H6 type had 6 ones such as 84H6, 86H6, 88H6, 90H6. 92H6 and 94H6. H6 type had 27 observations and frequency ratio of 19.57%. If lower body types were classified as same ones, these types could be graded up and down proportionately.

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Characterization of Soluble Type Human Folate Receptor on Folate Binding and Transport (Soluble type의 human folate receptor의 folate 결합과 수송에 관한 특성)

  • Kim, Won-Sin
    • Toxicological Research
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    • v.14 no.3
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    • pp.371-377
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    • 1998
  • We have cloned a soluble type human folate receptor(hFR type${\gamma}$) from human thymus cDNA library using the PCR amplification technique. To examine whether hFR type${\gamma}$ has a folate transport activity, CHO cells were transfected with the pcDNAhFR${\gamma}$ expression plasmid, and the stable cell line CHO/hFR${\gamma}$ expressing a high level of the hFR type${\gamma}$ was identified by northern and western blot analysis. The CHO/hFR${\gamma}$ cells produced a [$H^3$]folic acid binding protein in the culture medium. However, we couldn't detect any cell surface [$H^3$] folic acid binding and transport activities. The growth of the CHO/hFR${\gamma}$ cells was more rapidly inhibited than the wild type CHO cells in the low concentration folic acid media. These observations indicate that although soluble type human folate receptor can bind [$H^3$]folate, it does not involve in folate transport.

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Dialytic pH Condition for Obtaining Silk I Type Structure (Silk I형 결정을 얻기 위한 투석의 pH조건)

  • ;小西 孝
    • Journal of Sericultural and Entomological Science
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    • v.39 no.1
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    • pp.67-72
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    • 1997
  • As a condition for obtaining the silk I type crystal that has stability and high reproducibility, after dissolving silk fibroin crystalline part (Fcp), the changes of recrystallized crystal structure according to dialytic pH were examined by x-ray diffraction and differential thermal analysis. The Fcp was obtained from the aqueous solution of silk fibroin enzymatic proteolyzed by chymotrypsin. The crystal structure of Fcp showed silk II type. When the Fcp was dissolved by 10M LiBr aqueous solution, the Fcp1 showed the silk II type at pH 9. However, besides the silk II type, the silk I type structure begins to appear at pH 8 and only the silk I type structure was found below pH 6. On the other hand, the Fcp2 that calcium chloride was used in the dissolution found only the silk I type crystal structure below pH8.

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Characteristic Comparison of C-type and H-type Solenoid RF Chip Inductors (C-type과 H-type 솔레노이드 RF칩 인덕터의 특성 비교)

  • Yun, Eui-Jung;Kim, Jae-Wook;Kim, Yong-Suk;Lee, Tae-Bum;Hong, Chol-Ho;Jung, Young-Chang
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1524-1526
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    • 2002
  • 본 논문에서는 $1.62{\times}1.0{\times}0.88mm^3$ 크기의 C-type과 $1.58{\times}0.82{\times}0.94mm^3$ 크기의 H-type RF 칩 인덕터를 제작하고 그들의 고주파 특성을 비교하였다. 본 연구에서는 저손실 $Al_2O_3$ 코아 물질과 직경 $40{\mu}m$의 구리(Cu) 코일을 사용하였다. 권선수를 $2{\sim}14$회로 하여 인덕턴스(L), 품질계수(Q), 임피던스의 크기와 위상, 커패시턴스(C)를 HP4291B Impedance/Material Analyzer로 측정하였다. 10회 권선시 C-type은 55nH, H-type은 67nH, 14회 권선시 C-type은 100nH, H-type은 122nH 정도로 측정되었다. 실험 결과 H-type이 C-type보다 동일 권선수에 대하여 높은 인덕턴스와 높은 자기공진주파수(SRF)를 나타냄을 확인하였다. 또한 최대 품질계수는 두 형태가 거의 비슷한 값($55{\sim}87$)을 가짐을 관찰하였다. 따라서 H-type이 C-type보다 우수한 성질을 나타냄을 알 수 있다.

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Fabrication and Characterization of Miniaturized HTS Microstrip Antennas Using "H"-type Resonator (H 형태 공진기를 이용한 소형화된 HTS 안테나의 제작 및 특성 해석)

  • 정동철;윤창훈;황종선;최창주
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.52 no.7
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    • pp.282-287
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    • 2003
  • ″H″ type resonator has the advantage for the miniaturization of high-T7 superconducting (HTS) microstrip antenna in comparison with the conventional microstrip antenna such as rectangular type or circular type. In this paper we designed miniaturized HTS antennas using this "H"-type resonator and reported the characteristics of our antennas including return loss, bandwidth, radiation patterns, efficiency and so on. To fabricate the "H" type antenna, HTS YBa$_2$Cu$_3$$O_{7-x}$ (YBCO) thin films were deposited on MgO substrates using rf-magnetron sputtering. For comparison between normal conducting antennas and superconducting antennas, the gold antennas with the same dimension were also fabricated. An aperture coupling was used for impedance matching between 50 $\Omega$ feed line and HTS radiating patch. The ″H" type superconducting antenna showed the performance of 1.38 in SWR, 26 % in efficiency, and 13.8 dB in the return loss superior to the normal conducting counterpart.

Soil Properties Affecting C-type slope as a Parameter for Silica Sorption of Soils (토양의 규산 흡착 지표인 C-type slope에 영향을 미치는 토양 특성)

  • Lee, Sang Eun;Lim, Woo Jin;Ahn, Jae Ho;Kim, Jeong-Gyu;Lim, Soo-Kil
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.6
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    • pp.365-370
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    • 2004
  • To invesligate the characteristics of silica sorption on soils silica sorption experiments were conducted with 9 soils at 4 pH levels (5, 6, 7, and 8). Silica sorption increased in great extent with increase of pH. At the same pH level silica sorption increased linearly with increase of equilibrium $SiO_2$ concentration. Silica sorption characteristics was C-type. The C-type slope, i.e., the slope of linear regression of silica sorption isotherm, increased exponentially with increase of pH in all soils. Log(C-type slope) increased linearly with increase of pH in all soils. The slopes of linear regression were similar in most soils from 0.29 to 0.34 except Sachon and Jonggog soil. None of the soil properties showed any correlation with the slope of linear regression of Log(C-type slope) to pH. Only $Fe_o$ (oxalate extractable Fe oxides) was significantly correlated with the Log(C-type slope) at pH 7 in simple correlation analysis, and was shown to be the principal contributor as determined by standardized multiple linear regression.

A Study on Basic Pattern Evaluations Utilizing a Bodice Sloper Size Measurement Tool (원형치수 계산도구에 의한 바디스 원형 평가 연구)

  • Hong, Jiun;Kwon, Sookhee
    • Human Ecology Research
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    • v.54 no.4
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    • pp.427-440
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    • 2016
  • This study uses comparison tests of bodice basic patterns to research improvements in wearing evaluation methods based on differences in bodice basic patterns and body size. As study methods, we calculated body sizes for six types by selecting four types of bodice basic pattern and utilized bodice basic pattern size measurement tools. The study result are as follows. First, as the result of analyzing bodice basic patterns per each part and body size difference by bodice basic pattern size measurement tool, shoulder angle cover rate was J&K type 57.57%~68.41%, N type 53.13%~65.57%, L type 51.87%~63.73%, and H type 59.03%~68.20%. In the item of neck base circumference, it was J&K type 6.4~-16 mm, N type 10.7~-31.9 mm, L type -5.2~-13.4 mm, and H type -6.2~-15.4 mm. In the item of armscye circumference, it was J&K type -18.9~-59.4 mm, N type -15.2~-51.3 mm, L type -38.9~-52.7 mm, and H type -17~-42.2 mm. In the item of biacromion length, it was J&K type 23.2~-4.7 mm, N type 18.4~-10.4 mm, and H type 3.5~-5.7 mm. In the item of interscye back, it was J&K type and N type 17.6~-13.6 mm, L type 11.9~-13.6 mm, and H type 3.2~-11.8 mm. The item of interscye front was found in the sequences of study type and L type. Second, the Kruskal-Wallis test result (according to bodice basic pattern) indicated meaningful differences in items of shoulder angle cover rate, neck point to breast point, bust point-bust point, biacromion length and armscye circumference and H type had an overall higher rank.

pH and buffering capacity in some commercial fermented milks (일부 시판 유산균 발효유의 pH와 적정산도)

  • Ko, Seok-Ju;Jeong, Seong-Soog;Choi, Choong-Ho;Kim, Kyung-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.13 no.4
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    • pp.701-711
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    • 2013
  • Objectives : The aim of this study was to evaluate the pH and buffering capacity in some commercial fermented milks in Korea. Methods : The study was carried ouf from June to August, 2012. In 35 liquid type, 79 condense-stirred type and 71 condense-drink type fermented milks, available on the market, pH and buffering capacity were measured. Titration(with NaOH) was used to determine the buffering effect of each fermented milk. They were titrated with 1 M sodium hydroxide, added in 0.1 milliliters increments, until the pH reached about 5.5 and 7.0. Results : The average pH of tested fermented milks was $4.08{\pm}0.27$. The average pH values of fermented milks were $3.64{\pm}0.22$ in liquid type, $4.14{\pm}0.12$ in condense-stirred type, and $4.22{\pm}0.17$ condense-drink type. The average buffering capacity (pH 5.5) of tested fermented milk was $2.40{\pm}0.54$. The average buffering capacity (pH 5.5) of liquid type fermented milk was $2.37{\pm}0.33$, condense-stirred type fermented milk was $2.77{\pm}0.46$ and condense-drink type fermented milk was $2.01{\pm}0.42$. The average buffering capacity (pH 7.0) of tested fermented milks was $4.00{\pm}0.87$. The average buffering capacity (pH 7.0) of liquid type fermented milk was $3.11{\pm}0.36$, condense-stirred type fermented milk was $4.78{\pm}0.55$ and condense-drink type fermented milk was $3.58{\pm}0.59$. Conclusions : The average pH of tested fermented milks in this study was lower than pH 4.5. The type of fermented milks was an important factor for selection of fermented milk which is related with enamel erosion.