• Title/Summary/Keyword: H factor

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A Study on Characteristics of Pollutants from LNG Facilities (천연가스 사용설비 오염물질 배출특성 연구)

  • Lee, J.S.;Lee, H.C.;You, H.S.;Han, J.O.
    • 한국연소학회:학술대회논문집
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    • 2004.06a
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    • pp.223-226
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    • 2004
  • The thesis examined the charactcri:stlC of pollutants exhausted from LNG facilities. So, combustion gas and concentration of greenhouse gas exhausted from gas boiler for home use, furnace facilities, electric power facilities, boiler for industrial use and boiler for heating fueled LNG is measured. And the exhaust factor of pollutants is produced by classify of pollutants in detail. And this exhaust factor is compared and investigated with data of a foreign nations.

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Cloning and Nucleotide Sequence Analysis of the rpoH Gene from Methylovorus sp. Strain SS1 DSM11726 (Methylovorus sp. Strain SS1 DSM11726으로부터 rpoH 유전자의 클로닝과 염기서열 분석)

  • Eom, Chi-Yong;Song, Seung-Eun;Park, Mi-Hwa;Kim, Young-Min
    • Microbiology and Biotechnology Letters
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    • v.35 no.3
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    • pp.177-183
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    • 2007
  • Using complementation of RpoH deficient E. coli strain A7448, the rpoH gene encoding heat shock sigma factor 32 (${\sigma}^{32}$) from Methylovorus sp. strain SS1 DSM11726 was cloned and sequenced. Sequence analysis of a stretch of 1,796-bp revealed existence of an open reading frame encoding a polypeptide of 284 amino acid (32,006 dalton). Deduced amino acid sequence of the Methylovorus sp. strain SS1 RpoH showed that 59.6%, 39.1% and 51.4% identities with those of Nitrosomonas europaea (${\beta}$-proteobacteria), Agrobacterium tumefaciens ($\alpha$-proteobacteria) and E. coli (${\gamma}$-proteobacteria). The expression level of the functional ortholog of RpoH of Methylovorus sp. strain SS1 was increased transiently after heat induction, further indicating that it functions as a heat shock sigma factor.

pH Buffer Capacity and Lime Requirement of Korean Acid Soils (한국산성토양의 pH 완충력과 석회소요량 특성)

  • Kim, Yoo-Hak;Yoon, Jung-Hui;Jung, Beung-Gan;Zhang, Yong-Sun;Kwak, Han-Kang
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.6
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    • pp.378-382
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    • 2004
  • Soil pH is an important indicator for soil reactions and crop growth. pH buffer capacity and lime requirements are necessary to comprehend and manage soils well. The characteristics related with soil pH were analyzed and 5 field trials were conducted to elucidate pH buffer capacity of soil and lime requirements and liming factor for Korean acid soils. Soil minerals were analyzed for the soil of 2 years after treating $CaCO_3$ using X-ray diffraction. The amount of neutralized $H^+$ was regarded as the exchangeable aluminium overcoming ${\Delta}pH$, because pH buffer capacity of soil depended on exchangeable aluminium. Lime requirement was somewhat similar to the KCl exchangeable aluminium and it was also affected by the exchangeable cation by added lime. X-ray diffraction analyses revealed that an aluminium dissociation from Korean acid soils was equilibrated with kaolin minerals and changed into anorthite ($CaAl_2Si_2O_8$) by neutralizing with $CaCO_3$. Neutralizing process was composed of changing process of $Al^{3+}$ into $H^+$ and $Al(OH)_4{^-}$ ionic species and of neutralizing $H^+$ by, the amount of which was lime requirement. The fact that anorthite dissociates an aluminium ion higher than kaolinite does enabled to consider a liming factor (LF) the content of exchangeable cation and ${\Delta}pH$, $LF=1.5+0.2{\times}{\sum} Cations{\times}{\Delta}pH$.

Construction of Transgenic Silkworms Expressing Human Stem Cell Factor (hSCF) (인간 유래 Stem Cell Factor (hSCF) 재조합단백질이 발현되는 누에형질전환체 제작)

  • Kim, Sung-Wan;Yun, Eun-Young;Kim, Seong-Ryul;Park, Seung-Won;Kang, Seok-Woo;Kwon, O-Yu;Goo, Tae-Won
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1726-1731
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    • 2011
  • Human Stem Cell Factor (hSCF) is a cytokine that binds to the c-Kit receptor and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. To produce the human Stem Cell Factor (hSCF) recombinant protein, we constructed a germline transgenic silkworm using the piggyback vector. The expression of the hSCF gene was driven by the Drosophila heat shock protein 70 (dHsp70) promoter. 3XP3 promotor-driven EGFP was used as a marker which allowed us to rapidly distinguish the transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,020 eggs of bivoltin silkworms, Keomokjam. We obtained approximately 22 G1 broods that were EGFP-positive. The expression of the hSCF gene in the transgenic silkworm was analyzed by SDS-PAGE and immunoblotting. Also, analysis of insertion sites into the silkworm genome using inverse PCR showed that exogenous DNA was inserted into the transgenic silkworm genome. These results show that successfully constructed transgenic silkworm expresses the hSCF recombinant protein.

Pharmacokinetics of CJ-50001i Recombinant Human Granulocyte-Colony Stimulating Factor, in Rats and Dogs (CJ-50001 (recombinant human granulocyte-colony stimulating factor)의 흰쥐와 개에서의 약물동태학적 연구)

  • 김성남;신재규;이수정;정용환;하석훈;김기완;고형곤;김제학
    • Biomolecules & Therapeutics
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    • v.6 no.4
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    • pp.400-405
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    • 1998
  • The pharmacokinetics of CJ-50001 (recombinant human granulocyte-colony stimulating factor, developed by R&D center of Cheil Jedang Corp.) were investigated in rats and dogs. The serum concentrations of CJ-50001 were measured by a sandwich enzyme immunoassay. After single intravenous (iv) administration of Cf-50001 to rats at a dose of 5 $\mu$g/kg, the mean terminal half-life and area under the concentration-time curve (AUC) were 0.96 h and 124.497g . h/ml, respectively. After single subcutaneous (sc) administration at the same dose, maximum serum concentration was observed at about 2 hours after administration, and the mean terminal half-life, AUC and the bioavailability were 1.11 h,63.58$\mu$g . h/ml and 51.07%, respectively. In repeated dosing studies, CJ-50001 was administered iv and sc to rats at a daily dose of 5$\mu$g/kg for 7 days. The pharmacokinetic parameters, such as mean AUC and terminal half-life, were no significantly different from those of single administration. Following single iv and sc administration of CJ-50001 to dogs at a dose of 5 $\mu$g/kg, mean AUCs were much higher than those of rats, due to the decreased clearence (CL). After sc administration to dogs, maximum serum concentration was observed at 2~4 hours after administration and the bioavailability was 54.60%.

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Effects of Microstructure Morphology on Fluid Flow Characteristics of A356 Commercial Alloy in Semi-Solid Slurry (반고상 A356 합금 슬러리의 미세조직에 따른 유동특성에 관한 연구)

  • Kim, Jae-Min;Lee, Seung-Hoon;Hong, C.P.
    • Journal of Korea Foundry Society
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    • v.25 no.6
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    • pp.240-248
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    • 2005
  • The rheocasting characteristics are strongly influenced by the microstructural morphology such as particle size, form factor and contiguity. In this study, the effect of structural morphology on fluid flow characteristics of A356 semi-solid alloy was investigated with a vacuum suction fluidity test. Semi-solid metal slurry was made by the mechanical stirring, the liquidus casting, and H-NCM to be analysed. H-NCM could obtain uniform and fine globular microstructures of 0.9 form factor and 55 ${\mu}m$ particle size. Inoculation was found to be effective for reducing particle size, however, for H-NCM it should be avoided due to the cause of increasing contiguity. The fluidity test indicated that the non-stirring method had higher fluidity and smaller liquid segregation in the same solid faction of 0.4 than the stirring method, for smaller particle size and higher form factor. It was observed that liquid segregation decreased as the particle size is smaller and form factor is higher. The results of die-casting experiment were a good agreement with those of fluidity test.

High Voltage Ti/4H-SiC Schottky Rectifiers (고전압 Ti/4H-SiC 쇼트키 장벽 다이오드 제작 및 특성분석)

  • Kim, C.K.;Yang, S.J.;Lee, J.H.;Noh, I.H.;Cho, N.I.;Kim, N.K.;Kim, E.D.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07b
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    • pp.834-838
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    • 2002
  • In this paper, we have fabricated 4H-SiC schottky diodes utilizing a metal-oxide overlap structure for electric filed termination. The barrier height and Ideality factor were measured by current-voltage, capacitance-voltage characteristics. Schottky barrier height(SBH) were 1.41ev for Ni and 1.35eV for Pt, 1.52eV for Pt/Ti at room temperature and Pt/Ti Schottky diode exhibited Ideality factor was 1.06 to 1.4 in the range of $25^{\circ}C{\sim}200^{\circ}C$. To improve the reverse bias characteristics, an edge termination technique is employed for Pt/Ti/4H-SiC Schottky rectifiers and the device show excellent characteristics with higher blocking voltage up to 780V compared with unterminated devices.

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