• Journal of the Korean Society of Food Culture
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• v.21 no.2
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• pp.225-231
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• 2006

대황(Rhubarb)은 중국과 일본에서 혈액 순환제, 진통제, 신장치료제 등으로 전통적으로 사용되고 있는 약용식물이다. 이 연구에서는 한국산 R. undulatum L.의 뿌리 추출물, 미국산 R. rhabarbarum L.과 중국산 R. palmatum L.의 줄기 추출물의 두부 부패에 관여하는 미생물에 대한 항균 활성을 평가하였다. Rhubarb의 최적 추출을 위해서 각각 50%, 70%, 80% 에탄올을 용매로 사용하였으며, 항균 활성은 Kirby-Bauert test, minimum inhibitory concentration (MIC)과 minimum bactericide concentration (MBC)에 의해서 평가되었다. Kirby-Bauert test 결과, R. undulatum L.의 뿌리 추출물은 대부분 두부 부패균에 대해서 항균 활성을 가지는 것으로 나타났으며, R. rhabarbarum L.의 줄기 추출물의 경우는 $20{\mu}g/disc$의 농도에서는 항균 활성이 거의 나타나질 않았으나, 고농도로 갈수록 높은 항균 활성을 나타내었다. 또한 R. palmatum L.의 줄기 추출물은 고농도로 갈수록 항균 활성이 높아졌으나, Pseudomonas aeruginosa에 관해서는 항균 활성이 나타나지 않았다. MIC와 MBC에 의한 항균 활성 평가실험에서는, 세 종류의 rhubarb 추출물 중에서 phenolic 화합물이 가장 많이 함유되어 있는 R. undulatum L.의 뿌리 추출물보다 R. rhabarbarum L.의 줄기 추출물이 MIC와 MBC 값이 낮았다. 이는 phenolic 화합물의 양 이외에 낮은 pH가 항균 활성에 영향을 준 것으로 판단된다. 본 연구를 통해서 rhubarb 추출물은 두부 부패에 관여하는 미생물에 대해서 항균 활성을 가지는 것으로 나타났으며, 천연 항균제로써 식품에 적용 가능성이 있는 것으로 평가되었다. 87.1%이었으며, 아침을 매일 먹는 채식남학생은 78.8%, 비채식남학생은 33.3%, 채식여학생 47.1%, 비채식여학생 39.6%이었다. 또한 식사의 양은 과식한다는 응답이 채식남학생 24.2%, 비채식남학생 38.1%, 채식여학생은 29.4%, 비채식여학생 40.6%으로 비책식군의 과식율이 높았다. 5. 식품 섭취빈도는 두부 및 콩제품을 매일 섭취하는 경우는 채식남학생 54.6%, 비채식남학생 16.7%, 채식여학생은 38.2%, 비채식여학생이 16.8%이었다. 우유 및 유제품을 매일 섭취하는 경우는 채식남학생 6.1%, 비채식남학생 33.3%, 채식여학생 14.7%, 비채식여학생은 21.8%이었으며, 녹차, 커피 등 차를 마시지 않는다는 비율은 채식남학생 69.7%, 비채식남학생 28.6%, 채식여학생 29.4%, 비채식여학생 25.7%이었다. 인스턴트 식품을 매일 섭취한다는 응답율이 채식남학생 9.1%, 비채식남학생 21.4%, 채식여학생은 17.7%, 비채식여학생은 14.9%이었다. 6. 운동, 체중 조절 등에 대한 조사 결과 항상 운동을 하는 경우는 채식남학생 30.3%, 비채식남학생 28.6%, 채식여학생 14.7%, 비채식여학생 18.8%이었으며 운동시간은 $1{\sim}2$시간 하는 경우는 채식남학생 30.3%, 비채식남학생 38.1%, 채식여학생은 8.8%, 비채식여학생은 17.8%이었다. 체중에 만족하는 정도를 보면 채식남학생 57.6%, 비채식남학생 23.8%, 채식여학생은 23.5%, 비채식여학생은 15.8%가 만족한다고 하였다. 체중 조절 경험에서 경험이 있는 경우가 채식남학생 3.0%, 비채식남학생 31.0%, 채식여학생은 23.5%, 비채식여학생 31.7%이었다. 7. 골밀도 BQI값과와 몇가지 요인의 상관관계를 살펴보았을때, 채식남학생은 영양보충제의 섭취와 유의적인 양의 상관관계를, 해조류의 섭취정도와 유의적인 음의 상관관계를 나타내었다.

• Journal of Food Hygiene and Safety
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• v.38 no.3
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• pp.79-88
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• 2023

Trifludimoxazin is a triazinone herbicide that inhibits the synthesis of protoporphyrinogen oxidase (PPO). The lack of PPO damages the cell membranes, leading to plant cell death. An official analytical method for the safety management of trifludimoxazin is necessary because it is a newly registered herbicide in Korea. Therefore, this study aimed to develop a residual analysis method to detect trifludimoxazin in five representative agricultural products. The EN method was established as the final extraction method by comparing the recovery test and matrix effect with those of the QuEChERS method. Various sorbent agents were used to establish the clean-up method, and no differences were observed among them. MgSO₄ and PSA were selected as the final clean-up conditions. We used LC-MS/MS considering the selectivity and sensitivity of the target pesticide and analyzed the samples in the MRM mode. The recovery test results using the established analysis method and inter-laboratory validation showed a valid range of 73.5-100.7%, with a relative standard deviation and coefficient of variation less than 12.6% and 14.5%, respectively. Therefore, the presence of trifludimoxazin can be analyzed using a modified QuEChERS method, which is widely available in Korea to ensure the safety of residual insecticides.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.142-157
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• 2001

This study was conducted to get the basic information for promoting farm machinery productivity by surveying the regular maintenance and repair status of major farm machinery such as power tiller, farm tractor, rice transplanter and combine harvester. The survey was carried out through 9 provinces including Cheju province by direct visiting farmers with prepared questionnaire. The results of this study can be summarized as follows : 1. The average farming carrier of the surveyed farmers was 25.3 years, and 21-30 years of farming carrier showed the highest portion as 40.7%. The average carrier of using farm machinery was 9.4 years, and that was 14.9 years for power tiller, 8.3 years for farm tractor, 9.0 years for rice transplanter, 7.9 years for combine harvester, 7.5 years for mini tiller, 9.7 years for power sprayer, and 8.2 years for binder etc. 2. The regular maintenance for farm machinery was conducted mainly at repair shop (49.5%) or dealer agency (12.0%) as 61.5%, and 34.9% of farmers conducted the regular maintenance by themselves at their house. 3. The reasons for not-fully recognizing operation manual and insufficient before-, during-, after-maintenance of farm machinery were insufficient time for them (45.8%), troublesome (22.9%), unknown maintenance method (16.3%), unknown the necessity for maintenance (12.4%), and others (2.6%) in order. 4. For the annual exchange of engine oil, 3.2 times is necessary but actually 1.7 times was exchanged for power tiller, 4.3 times is necessary but actually 1.9 times was exchanged for farm tractor, 2.7 times is necessary but actually 1.7 times was exchanged for rice transplanter, 2.2 times is necessary but actually 2.3 times was exchanged combine harvester. 5. For the annual cleanness or exchange of fuel filter, 3.2 times is necessary but actually 1.1 times was done for power tiller, 4.3 times is necessary but actually 1.6 times was done for farm tractor, 2.7 times is necessary but actually 1.7 times was done for rice transplanter, 1.9 times is necessary but actually 0.8 times was done for combine harvester. 6. For the annual cleanness or exchange of air filter, 3.2 times is necessary but actually 1.4 times was done for power tiller, 4.2 times is necessary but actually 2.4 times was done for farm tractor, 2.6 times is necessary but actually 1.6 times was done for rice transplanter, 3.9 times is necessary but actually 7.0 times was done for combine harvester. 7. For the experience of breakdown related to maintenance, 5.3% of farmers experienced breakdown due to the insufficient exchange of engine oil, 7.7% of farmers experienced breakdown due to the insufficient cleanness or exchange of fuel filter, and 2.9% of farmers experienced breakdown due to the insufficient cleanness or exchange of air filter. 8. Most farmers (76.1%) recognized the necessity for agricultural machinery training or education, and most farmers preferred about one week for the training period, simple or ease maintenance for the training level, agricultural technical center or agricultural machinery manufacturer for the training agency. 9. Complete recognition of operation manual and sufficient before-, during-, and after-maintenance for farm machinery can minimize the breakdown as well as conduct suitable period farming, enlarge the endurance, prevent the safety accidents, and promote productivity of farm machinery. Therefore, these can be accomplished by the thorough training or education for agricultural machinery.

• Journal of Naturopathy
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• v.7 no.1
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• pp.10-19
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• 2018

The purpose of this study was to investigate the distribution and variation of the anion and cation number in the aerosols at 16 sites in the Jeju area. The average value of anion counts was raged from 449.35 ions/cm³ at Jeju city to 3471.25 ions/cm³ in the Chunjiyeon falls. In order, the lowest Jeju-si < hamdeok < 1100 m < farm < gyorae A < saryoni < jeolmul < gyorae B < geomunoreum < halla forest < hallasan garden < seongpanak < dongbaeksan < jeongbang < wonyang, respectively. There was statistically significant difference between the anion counts of the measured values in the order of elevation. The mean value of cation measurements was from 90 ions/cm³ for Cheonjiyeon, to 729.8 ions/cm³ for Halla forest garden, which showed the highest value. In order, the lowest 729.8 ions/cm³ of Cheonjiyeon < 1100 m < dongbaeksan < Jeju-si < saryoni < wonyang < seongpanak < hamdeok < jeongbang < gyorae B < jeolmul < Farm < gyorae A < halla forest < geomun and < halla garden, respectively. The geographically low area and the high area were measured low and difference in the two ions. The differences between the content of the anion, cation and/or altitude were significant each other in the variance analysis. The correlation between the anion and cation content and/or altitude was statistically significant (r=.396, p<.001). In conclusion, the result of showing the temporal distribution and variation of the anion and cation content in the aerosols in Jeju island forests provides important information for healthcare.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.49-59
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• 2019

The field trial was performed to evaluate the rice growth and yield in different direct seeding methods after Italian Ryegrass Harvest The required time for seed emergence was for 7 ~ 8days in the tested direct seeding methods and there was high in seedling establishment in order of wet hill-seeding with iron-coated seeds > water seeding with iron-coated seeds > wet hill-seeding with soil coverage with pregerminated seeds. The rice plant height was shorter in the tested direct seeding methods than that of machine transplanting until 45day after seeding but there was not significant difference in terms of statistical analysis at 63day after seeding. The growth of tiller number in the rice plant was high in water seeding with iron-coated seeds and wet hill-seeding with soil coverage and low in wet hill-seeding with iron-coated seeds compared to machine transplanting. The yield component in the tested direct seeding methods was not significant difference in terms of statistical analysis. The milled rice yield in the tested direct seeding methods was higher 2 ~ 8% being with 4.94 ~ 5.24t/ha than that of machine transplanting but there was not significant difference in terms of statistical analysis. The percentage of head rice was low in the tested direct seeding methods compared to machine transplanting. The weedy rice was not occurred in the tested rice cultivation methods. In conclusion the direct seeding method would be recommended to be a suitable to in following by Italian ryegrass harvesting in southern area of Korea in terms of reduction in production cost and high income basis for rice growing farmers.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.5-18
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• 2018

The field trial was performed to evaluate the rice growth and yield in direct seeding cultivation with iron-coated rice seeds. The required time for seed emergence was for 9~11days in the tested direct seeding methods. That was 1~2days earlier in direct seeding with pregerminated seeds than that of direct seeding with iron-coated seeds. The seedling establishment was highest in water seeding with iron-coated seeds but there was not significant difference in terms of statistical analysis. The rice plant height was taller in water seeding with broadcasting method than that of wet hill-seeding methods and in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The tiller number in the rice plant was the highest in machine transplanting at 30days after direct seeding(June 17) and in water seeding with iron-coated seeds at 45days after seeding(DAS) and 60DAS. The tiller number of 75 and 90DAS in the tested rice cultivation methods being with 352~405/m² was not significantly different in terms of statistical analysis. The heading time was not different in rice direct seeding methods but 2 day earlier in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The culm length was the highest in water seeding with iron-coated seeds and the panicle length was the longest in wet hill-seeding with pregerminated seeds. The panicle number per m² was highest in water seeding with iron-coated seeds but not significant difference among the tested rice cultivation methods. The water seeding with iron-coated seeds resulted in the highest spikelet number per m² and the heaviest grain weight of brown rice. Percentage of ripened kernel was the highest in wet hill-seeding with iron-coated seeds. But there were not significant among the tested rice cultivation methods. The milled rice yield in direct seeding methods was 3~21% higher than that in machine transplanting. Water seeding with iron-coated seeds recorded the highest milled rice yield being with 6.86t/ha.The occurrence of sheath blight was high according to machine transplanting>wet hill-seeding>water seeding. Weed occurrence was the highest in water seeding with pregerminated seeds. Weedy rice occurred not in machine transplanting but occured 0.6~0.7% in direct seeding methods with pregerminated seeds and 0.1% in direct seeding with iron-coated seeds.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.61-70
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• 2019

"Jungmo2510", a rye cultivar, Secale cereal L., was developed by National Institute of Crop Science, RDA in 2015. It was developed from open pollination from within 10 rye cultivars or lines including "Chochun" in 1995. The line "SR95POP-S1-523-1-5-5-4-7-3-B-16-3-19" was selected for its excellent agronomic appearance and was placed in yield trials for two years from 2011 to 2012. The line was designated "Homil55" and was placed in regional yield trials at the four locations around Korea from 2013 to 2015, during which time the name "Jungmo2510" was given. This cultivar is an erect plant type and leaves of short and broad size with a green color, a yellow colored, medium-diameter culm, and a yellowish brown-colored, medium-size grain. The heading date of "Jungmo2510" was April 16, which were 2 days earlier than that of "Gogu". "Jungmo2510" also showed similar to winter hardiness and greater resistance to lodging compared to those of the check cultivar. Over three years, the average dry matter yield of "Jungmo2510" was 802 kg 10a^-1 , which was harvested in late April and was lower than that of the check cultivar "Gogu" (825 kg). The seed productivity of "Jungmo2510" was approximately 481 kg 10a^-1 , which was 2.4% less than that of the check. "Jungmo2510" was higher to than "Gogu" in term of protein content (9.1% and 8.0%, respectively), total digestible nutrients(TDN)(57.5% and 55.5%, respectively), and TDN yield 10a^-1(419 kg and 392 kg, respectively). This cultivar is recommended as a fall sowing crop in areas where the average daily minimum-mean temperatures are higher than -12 ℃ in January, and as a winter crop for whole-crop forage before the planting of rice or green manure around Korea.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.103-103
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• 2022

다복찰은 전라북도농업 기술원에서 2012~2020년에 신명흑찰과 익산488호를 교배하여 육성한 찰벼이며, 중대립, 단간, 내도복, 다수성의 특징을 보인다. 동진찰은 1998년도에 육성되었으며, 우리나라에서 가장 인기 있는 찰벼이다(재배면적 25,161ha/2021년 기준). 드문모심기 재배 기술은 2017년부터 도입되었으며, 밀파 육묘하여 재식밀도 및 재식주수를 감소함으로써 소요되는 재료비 등 경비 및 투입되는 노동력을 절감하는 경제성 있는 기술이다. 따라서 본 연구는 전라북도농업기술원에서 육성한 다복찰 보급 확대 및 가장 인기 높은 동진찰의 드문모심기 재배기술 확립 일환으로 시작되었으며, 2024년까지 3년간 실시할 계획이다. 시험은 기술원 논 포장에서 실시하였으며, 시험품종은 다복찰, 동진찰, 시험요인은 이앙시기(3처리) 및 재식밀도(4처리)를 두었다. 이앙시기별로 이앙 17일 전에 온탕소독(62℃, 10분), 종자소독(32℃, 1일), 침종(32℃, 1일), 간이출하(30℃, 3일) 과정을 거쳐 부직포 육묘(12일 정도)를 실시하였다. 5월20일(이앙520)부터 5월30일(이앙530), 6월9일(이앙609)까지 10일 간격으로 드문모심기 전용 이앙기로 이앙하였으며, 주당 본수는 5.7개 정도였다. 이앙 시기별로 시험포장을 구분하였고 포장내에 2품종, 각각 3.3m2당 80주, 60주, 50주, 37주 등 재식밀도 처리구를 두었으며, 처리구마다 3반복 조사구(10주/반복, 5본/주) 설치하였다. 10a당 9-4.5-5.7kg(N-P₂O₅-K₂O)를 밑거름(50%)-분얼거름(20)-이삭거름(30) 등 3회 거쳐 시비하였다. 중간물떼기는 이앙32일째부터 10일간 실시하였으며, 예상 출수 30일 전에 충분하게 담수하였다. 이앙20일째부터 10일 간격으로 경수, 초장, 엽색도 등 생육 조사, 그리고 출수기, 후기 생육 및 병해충을 조사하였다. 향후 수확기에 수량, 수량구성요소, 미질 및 품위를 분석할 계획이다. 시험토양은 pH 6.0~6.3, EC는0.68~0.85dS/m, 유기물함량은 52~57g/kg 수준으로 높았다. 동진찰 발아율(94.0~98.1%)이 다복찰(89.9~94.9%)보다 우수하였다(3~6%P ↑). 다복찰 묘 충실도(102~106mg/주)가 동진찰(79~101mg/주)보다 다소 좋았으며, 이앙530 묘소질이 가장 좋았다. 동진찰 초장은 다복찰에 비해 다소 길었으나 이앙시기 및 재식밀도별 초장의 변화 유형은 비슷하였다. 이앙520구의 80주에서 초장이 가장 작았고 이앙609구에서는 80주에서 다소 길었으나, 처리구간의 유의성은 없었다. 동진찰 및 다복찰 경수는 37주에서 이앙 후 40일째, 50주, 60주, 80주에서는 30일째 가장 컸다. 출수기는 다복찰에 비해 동진찰이 3~7일 정도 빨랐으며, 특히 이앙520 동진찰에서는 재식밀도가 높을수록 출수기가 빠른 경향을 보였으며, 이앙530 이후에는 재식밀도간 차이가 거의 없었다. 동진찰에 비해 다복찰 간장이 3~6cm 작았으며, 2품종 모두 이앙609구에서 가장 낮은 값을 보였다. 수장도 간장과 비슷한 경향을 보였다. 수수는 2품종 모두 이앙이 늦을수록 증가하였으며, 37주에서 가장 높았으며, 80주에 비해 다복찰, 동진찰 각각 73.8%, 77.4% 높았다. 다복찰의 경우 3.3m²당 수수는 이앙시기별과 관계없이 상대적으로 80주에서 가장 많았고 이앙520에서 재식밀도간 차이는 감소하였지만 이앙시기가 늦을수록 수수는 증가하는 경향을 보였다. 동진찰도 비슷한 경향을 보였으며, 이앙520과 이앙609 사이에 수수 차이는 적었다. 병 발생은 잎집무늬마름병, 세균성벼알마름병, 이삭누룩병이 주로 관찰되었으며, 세균성 벼알마름병은 출수기와 맞물린 이앙530에서 가장 크게 발생하였으며, 이앙520도 비슷한 경향을 보였다. 이삭누룩병 발생이 심하였는데 재식밀도간 차이보다는 이앙시기별 차이가 더 크게 발생하였으며 품종 간의 병 발생 차이는 없었다. 이상의 결과로 수수 측면에서 조기 이앙할 경우에는 50~60주, 늦은 이앙 시에는 80주가 유리할 것으로 보이며, 추후주당 립수, 등숙률, 천립중 등을 조사하고 경영비 등을 고려하여 합리적인 이앙시기 및 재식밀도를 판단할 수 있을 것으로 판단된다.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.4
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• pp.268-273
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• 2023

The present study estimated rumen fermentation characteristics and greenhouse gas emissions of different forages. Alfalfa, timothy, tall fescue, Italian ryegrass, and rice straw as the main forage sources for Hanwoo were used in the present study. Crude protein was highest in alfalfa but lowest in rice straw (p<0.05). Ether extract was higher in alfalfa and Italian ryegrass than in the other forages (p<0.05). Crude ash was highest in rice straw but lowest in tall fescue (p<0.05). Neutral detergent fiber was highest in tall fescue but lowest in alfalfa (p<0.05). Acid detergent fiber was highest in Italian ryegrass and rice straw but lowest in alfalfa (p<0.05). In vitro digestibilities of dry matter (DMD) and neutral detergent fiber (NDFD) were highest in timothy but lowest in rice straw (p<0.05). Rumen pH was highest (p<0.05) in alfalfa, while ammonia-N was higher (p<0.05) in alfalfa and Italian ryegrass than in the other forages. Total volatile fatty acid was highest (p<0.05) in timothy, while acetate and propionate were highest (p<0.05) in alfalfa and rice straw, respectively. Acetate to propionate ratio was higher (p<0.05) in alfalfa, timothy, and Italian ryegrass than in rice straw. Rice straw had lowest total gas (mL) (p<0.05) but highest its per DMD and NDFD. Rice straw had higher (p<0.05) CO₂ (per DMD and NDFD) compared to alfalfa (per DMD and NDFD), timothy (per DMD and NDFD), tall fescue (per NDFD), and Italian ryegrass (per DMD). Again, rice straw had higher (p<0.05) CH₄ (per DMD and NDFD) compared to timothy (per DMD and NDFD) and tall fescue (per NDFD). Therefore, this study indicates that timothy has a higher nutrient digestibility and volatile fatty acid in the rumen leading to a reduction of greenhouse gas emission.

• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.455-468
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• 2023

We aimed to assess the potential growth-promoting effects of buckwheat sprout on intestinal bacteria and their anti-inflammation effects in a cellular model of intestinal inflammation. The growth of Bifidobacterium longum ssp. infantis BT1 was enhanced with the addition of the sprout extract of tartary buckwheat. Further, in the inflammatory model cells cultured with Raw 264.7 cells were treated with buckwheat sprout including each 10 probiotics before the addition of lipopolysaccharide (LPS) to induce inflammation in Raw 264.7 cells. Buckwheat sprout in both Bifidobacterium longum ssp. infantis BT1 and Lacticaseibacillus paracasei LPC5 significantly reduced the production of NO and PGE₂. The above results indicate that buckwheat sprout extract which contains with various physiologically active substances such as rutin, quercetin, and choline is effective in suppressing NO and PGE₂ production, which are inflammation-related indicators. The present study suggests that buckwheat sprout could induce positive effects on the intestinal beneficial bacteria and in anti-inflammation.