• Title/Summary/Keyword: Gyeongbuk

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경북 동부지역 꿀벌에서 주요 병원체의 분자생물학적 검출 (Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea)

  • 우인옥;도재철;서민구;정태남;조민희;곽동미
    • 한국동물위생학회지
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    • 제36권1호
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    • pp.37-44
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    • 2013
  • The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.

경북지역 산란계에서 Avian pneumovirus에 대한 항체가 및 바이러스 검출 조사 (Examination of seroprevalence and detection of Avian pneumovirus from layer hens in Gyeongbuk province)

  • 김정은;황지영;배동록;성명숙;김순태;김상윤
    • 한국동물위생학회지
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    • 제30권1호
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    • pp.43-49
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    • 2007
  • Avian pneumovirus(APV), also known as avian rhinotracheitis(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis(TRT). The aim of this study was to establish the presence or absence of antibodies to APV by an enzyme-linked immunosorbent assay(ELISA) and confirm APV by a reverse transcriptase-polymerase chain reaction(RT-PCR). The tested serum and feces were collected from laying hens in Gyeongbuk province. The positive farms with antibody against APV by ELISA were 90(96.7%) of 93 and positive serum samples were 433(93.1%) of 465 different sera. By regional group, sera from Uiseong, Cheongsong and Bonghwa were noted as 100% positive and positive rates of samples from Yeongju, Andong and Yeongyang were 93.3%, 85.7% and 50%, respectively. However, APV was not detected in feces samples by RT-PCR.

영천지역 구제역 바이러스 구조단백질 항체가 조사 (Survey of foot-and-mouth disease virus structural protein antibody titer in Yeongcheon)

  • 손준형;황유선;손규희;신성호;이은미;김순태;조민희;윤문조
    • 한국동물위생학회지
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    • 제38권1호
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    • pp.13-17
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    • 2015
  • Three serotypes (O, A and Asia1) of the foot-and-mouth disease (FMD) vaccine were injected into domestic cloven-hoofed animals in korea after the nationwide spread at the end of 2010. The purpose of this study was survey of FMD virus stuructural protein (SP) antibody titer in Yeongcheon by enzyme-linked immunosorbent assay (ELISA). Total 1,324 samples collected from 89 farms were tested. The overall seroprevalence of FMD virus SP antibodies was 58.8% (778/1,324) The seroprevalence of FMD virus SP antibody varied with species. Results in cattle (over 12 month old) and pig (90 to 130 day old) were 58.8% and 44.9% respectively.

경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적특성 및 PFGE 양상 (Biochemical characterization and PFGE pattern of Brucella canis isolated from kennels in Gyoengbuk province)

  • 김성국;김영환;홍현표;엄현정;장성준;조민희;이양수
    • 한국동물위생학회지
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    • 제30권3호
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    • pp.363-374
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    • 2007
  • A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143 (53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711 bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of $H_2S$, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme Smi I, 25 isolates tested were typed to 2 pattern, S1 and S2.