Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Biochemical characterization and PFGE pattern of Brucella canis isolated from kennels in Gyoengbuk province

경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적특성 및 PFGE 양상

  • 김성국 (경상북도 가축위생시험소) ;
  • 김영환 (경상북도 가축위생시험소) ;
  • 홍현표 (경상북도 가축위생시험소) ;
  • 엄현정 (경상북도 가축위생시험소) ;
  • 장성준 (경상북도 가축위생시험소) ;
  • 조민희 (경상북도 가축위생시험소) ;
  • 이양수 (경상북도 가축위생시험소)
  • Published : 2007.09.30
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Abstract

A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143 (53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711 bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of $H_2S$, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme Smi I, 25 isolates tested were typed to 2 pattern, S1 and S2.

Keywords

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