• Development and Reproduction
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• v.23 no.4
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• pp.377-384
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• 2019

Genomic DNA extracted from representatives of two populations, Gunsan and Chinese, of Urechis spp. was amplified using PCR with several primers. The band-sharing (BS) value between individuals no. 05 from the Gunsan population and no. 22 from the Chinese population was 0.206, which was the lowest recognized value. Oligonucleotides primer OPC-04 revealed 44 unique loci, which distinguished the Chinese population. Primer OPB-17 allowed the discovery of 22 loci shared by the two populations, which were present in all samples. Based on the average BS results, individuals from the Gunsan population demonstrated lower BS values (0.661±0.012) than did those from the Chinese population (0.788±0.014; p<0.05). The shortest genetic distance (GD) displaying a noteworthy molecular difference was between individuals CHINESE no. 12 and no. 13 (GD=0.027). Individual no. 06 from the Gunsan population was most distantly related to CHINESE no. 22 (GD=0.703). A group tree of the two populations was constructed by UPGMA Euclidean GD analysis based on a total of 543 fragments generated using six primers. The explicit markers recognized in this study will be used for genetic analysis, as well as to evaluate the species security and proliferation of echiuran individuals in intertidal regions of the Korean Peninsula.

• Korean Journal of Clinical Laboratory Science
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• v.43 no.1
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• pp.26-31
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• 2011

The genotypes of Human Papilloma Virus (HPV) are important in the carcinogenesis of uterine cervical cancer. Diagnosis of uterine cervical cancer screening has been executed using Papanicolau method (Pap) and HPV DNA Chip method. We researched the interrelation of HPV DNA genotypes in single and multiple infections and analyzed the results of Pap and HPV DNA Chip tests at Gunsan Medical Center (GMC). The correlation analysis was surveyed on collected results from 599 patients who have been tested with both Pap and HPV DNA chip tests from November 2004 to May 2010 at GMC. The inconsistency between Pap and HPV DNA Chip tests was 41.1%. The HPV DNA Chip genotype related with high risk cases were type 16 (13.5%), type 52 (10.5%), type 58 (10.1%), and type 18 (3.4%). Those related with low risk cases were type 70 (8.9%), type 6 (1.7%), type 40 (1.2%), type 11 (1.3%), and other types (14.3%). Among the 195 cases of HPV positive status, 161 cases were associated with single infection; 108 (67.1%) cases were related with high risk genotype; 19 (11.8%) cases were low risk genotype; 31 (21.1%) cases were related with other types. 29 cases were associated with double infections; 23 (79.3%) cases were high risks; 5 (17.2%) cases were mixed high and low risks; 1 (3.5%) case was low risk.

• Journal of the Korean association of regional geographers
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• v.15 no.3
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• pp.351-368
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• 2009

The internal structure of gateway city is to be influenced by the external relations. The purpose of this study is to examine the transportation impacts on the spatial characteristics of gateway cities in the Geum River basin, Korea. The changes of the transportation is classified by the commodity, the rice flow, and the functions of the gateway are examined in Gunsan-Si and Ganggyeong-Eup. Major findings of the study are as follows. First, the water and land transports in the gateways are connected or not. In the flow of the rice, the Gunsan gateway is connected, while the Ganggyeong is not. The difference is reflected in the location of the port and railway station. In the flow of marine products and salt, the Ganggyeong gateway is connected, while the Gunsan is not. Second, there had been separated or not in the location of the ports of the marine products and the rice. They are separated in the Gunsan, while not separated in the Ganggyeong. The results imply that there are to be difference between the two ports after the gateway of rice.

• Development and Reproduction
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• v.13 no.2
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• pp.97-103
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• 2009

Genomic DNA samples isolated from four geographical freshwater crab (Eriocheir sinensis) populations collected in the inland of the Korean Peninsula (Gunsan, Paju, and Nampo) and a Chinese site, were used for PCR amplification. Seven decamer primers generated 19 specific loci (19/243 loci, 7.81%) in the Gunsan population, 32 (32/215 loci, 14.88%) in the Paju population, 19 (19/231 loci, 8.23%) in the Nampo population and 62 (62/340 loci, 18.24%) in a Chinese population. The average 8.9 specific loci exhibited inter-individual-specific characteristics, thus revealing DNA polymorphisms in the Chinese population. The number of unique shared loci to each population and number of shared loci by the four populations were generated by molecular analysis using seven primers in four populations. 35 unique shared loci to each population, with an average of 5.0 per primer, were observed in the Gunsan population, and 50 loci, with an average of 7.1 per primer, were observed in the Chinese population. The hierarchical dendrogram indicates three main branches: cluster 1 (GUNSAN 01$\sim$GUNSAN 05, PAJU 06$\sim$PAJU 10 and NAMPO 11$\sim$NAMPO 15) and cluster 2 (CHINESE 16, 17, 18, 19 and 20). Conclusively individual no. 20 of the PAJU 10 freshwater crab was most distantly related to CHINESE no. 20 (genetic distance = 0.667). Taken together, these results demonstrate the potential of RAPD analysis to identify diagnostic markers for the identification of four freshwater crab populations.

• Journal of agricultural medicine and community health
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• v.44 no.4
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• pp.185-194
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• 2019

Objective: In Gunsan, Jeollabuk-do, Korea, we wanted to determine if the sluggish local economy could affect citizens' health behaviors, especially mental health. Methods: We divided Gunsan-si into 5 living areas and conducted Small-Area Estimations and confirmed the modified compound estimation value using the 2013-2017 Community Health Survey data and population data from Gunsan-si. Results: The health behaviors and mental health of the residents of the western living area(Soryong-dong, Misung-dong), which is an industrial hub of Gunsan, had deteriorated or decreased compared to those of other regions. Conclusions: Although there are limitations in analyzing the community health survey data using the small-area estimation method, it could be useful data for evaluating regional gaps and health level.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.77-82
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• 2007

선박의 해난에 의한 해양오염사고 발생시, 해양경찰은 민관 방제세력의 동원 및 방제조치, 방제지원시스템을 운용 등을 통하여 신속하고 체계적인 방제전략을 수립 시행함으로써, 사고로 인한 해양환경 피해를 최소화하고 있다. 본 연구에서는 2006년 7월 전북 군산시 새만금방조제 인근 해상에서 침몰한 700톤급 준설선 GA-P1 호에 대한 선체인양 및 해양오염사고 처리 과정에서 방제지원시스템을 이용한 대응 사례이다.

• Journal of Fisheries and Marine Sciences Education
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• v.20 no.3
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• pp.487-499
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• 2008

This study was analyzed and assessed the environmental factors on small marine ranching sites around Gunsan Coast, West Sea of Korea. Standards of assessment are followed in the reports on the choice of marine ranching sites and regulation factors of proper site on marine artificial reefs of MOMAF. Max. current speed around Gunsan were ranged between 25.52 ~ 57.49cm/sec. These values were satisfied by comparing assessment factor such as current speed.. Factors of temperature, salinity, transparency and pH were evaluated as the proper extent. DO was not distributed as the proper extent due to different criterion. between the same assessment factor. COD, SS and nutrients were partly suited to the criteria. Especially, SS was almost higher than that of criterion. So, we are proposed to be reflected in environment characteristics of West Coast such as SS. Moreover, the extent of pollution load factors have to be specified clearly in the regulations of assessment criteria.

• Proceedings of the Korea Information Processing Society Conference
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• 2000.10b
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• pp.1497-1500
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• 2000

본 논문은 한글 고유 명사를 새로 개정된 한글 로마자 표기법에 의해 로마자 표기로 변환하는 시스템의 구성에 대해 기술하였다. 1995년에 문화체육부에서 배포한 프로그램 이후로 현재까지 한글을 로마자 표기로 자동 변환하는 프로그램이 나오지 않았고 이 프로그램 또한 새로 개정된 로마자 표기법이 아닌 예전의 로마자 표기법을 따르는 이유로 본 논문은 새로 개정된 표기법을 따르는 한글 고유명사의 로마자 자동 변환 시스템을 DB를 이용하여 구축하는 방안을 제시하였다. 그 결과 DB를 이용한 한글의 로마자 자동변환시스템은 개발자 측면에서는 프로그램 구현과 정확성 면에서 효과적인 장점을 얻을 수 있으며, 사용자 측면에서는 새로 개정된 개정안을 쉽게 익히고, 사용할 수 있게 한다.

• Annual Conference on Human and Language Technology
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• 2000.10d
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• pp.76-81
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• 2000

본 논문은 한글 고유 명사를 새로 개정된 한글 로마자 표기법에 의해 로마자 표기로 변환하는 시스템의 구성에 대해 기술하였다. 1995년에 문화체육부에서 배포한 프로그램 이후로 현재까지 한글을 로마자 표기로 자동 변환하는 프로그램이 나오지 않았고 이 프로그램 또한 새로 개정된 로마자 표기법이 아닌 예전의 로마자 표기법을 따르는 이유로 본 논문은 새로 개정된 표기법을 따르는 한글 고유명사의 로마자 자동 변환 시스템을 DB를 이용하여 구축하는 방안을 제시하였다. 그 결과 DB를 이용한 한글의 로마자 자동변환시스템은 개발자 측면에서는 프로그램 구현과 정확성 면에서 효과적인 장점을 얻을 수 있으며, 사용자 측면에서는 새로 개정된 개정안을 쉽게 익히고, 사용할 수 있게 한다.

• Development and Reproduction
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• v.19 no.4
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• pp.259-264
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• 2015

Genomic DNA samples isolated from geographical purplish Washington clam (Saxidomus purpuratus) were obtained from three different regions in the Korean Peninsula: Geoje (Geoje population; GJP), Gunsan (Gunsan population; GSP) and a site of North Korea (North Korea population; NKP). The seven primers generated the total 369 loci that can be scored from the GSP clam population. 356 fragments were generated from the NKP clam population. The complexity of the banding patterns varies dramatically between the primers and three localities. In this study, 319 loci were identified in the purplish Washington clam from Geoje and 369 in the clam population from Gunsan: 221 specific loci (69.3%) in the GJP clam population and 300 (81.3%) in the GSP population. These results demonstrate that the primer detected a large quantity of specific fragments, suggesting that the genetic variation in the GSP is higher than in the GJP population. In particular, the BION-28 primer gave DNA profiles with more fragments than the other six primers in the NKP population. The oligonucleotides primer BION-75 produced 21 unique loci to each population, which were ascertaining each population, approximately 250 bp, 300 bp and 400 bp, in the GJP population. Outstandingly, the primer BION-50 detected 21 shared loci by the three populations, major and/or minor fragments of sizes 150 bp, which were matching in all samples. With regard to average bandsharing value (BS) results, individuals from GJP population (0.743) displayed higher bandsharing values than did individuals from GSP population (0.606). In the present study, the dendrogram gained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (GEOJE 01 ~ GEOJE 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14), cluster 3 (N.KOREA 15 ~ N.KOREA 21). Among the twenty one clams, the shortest genetic distance that revealed significant molecular differences was between individuals 08 and 09 from the NKP population (genetic distance = 0.073), while the longest genetic distance among the twenty-one individuals that demonstrated significant molecular differences was between individuals GEOJE no. 03 and GUNSAN no. 09 (genetic distance = 0.669). Comparatively, individuals of GJP population were properly closely related to that of NKP population, as revealed in the hierarchical dendrogram of genetic distances. In due course, PCR analysis has revealed the significant genetic distance among three purplish Washington clam populations. PCR fragments discovered in this study could be valuable as a DNA marker of the three geographical clam populations to distinguish.