• 한국발생생물학회지:발생과생식
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• 제3권1호
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• pp.95-100
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• 1999

Growth/differentiation factor-9 (GDF-9)은 transforming growth factor $\beta$ (TGF-$\beta$) superfamily의 member로서 난소의 난자에서만 특이적으로 발현되며 정상적인 난포발달에 있어 필수적인 성숙인자로 최근에 알려졌다. 본 연구는 RT-PCR을 통해 생쥐의 원시난포에서의 GDF-9 mRNA의 발현 여부와 함께 난포의 발달단계에 따른 상대적인 발현량을 분석하고자 실시하였다. 본 실험에는 ICR 생쥐를 사용하여 질전 (vaginal plug)이 확인된 날을 1일로 하여 임신 19일의 태아와 태어난 날을 1일로 하여 생후 1일, 10일, 21일, 28일된 생쥐 난소를 실험에 사용하였다. 각 발달단계의 난소조직으로부터 total RNA를 추출하여 GDF-9 유전자 발현 여부를 확인하였으며 이들을 $\beta$-actin에 대해 상대적인 정량분석을 하였다. GDF-9 유전자 발현은 아직은 성장을 시작하지 않은 임신 19일의 태아의 난소, 대부분이 원시난포로 이루어진 태어난 날의 생쥐 난소에서도 확인되었으며, 성장이 왕성하게 진행되고 있는 난포 즉, antrum 형성 이전의 growing follicles이 주를 이루는 생후 10일째의 난소에서 가장 높은 GDF-9 유전자 발현이 관찰되었다. 나머지 단계의 난소에서는 거의 비슷한 정도로 발현함을 관찰할 수 있었다. 본 연구의 결과는 생쥐의 원시난포에도 GDF-9 transcript가 존재한다는 것을 확실하게 증명하였으며, GDF-9이 생쥐의 초기 난포발달에 중요한 역할을 할 것이라는 가능성을 시사한다.

• 성인간호학회지
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• 제25권3호
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• pp.312-321
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• 2013

Purpose: The purpose of this study was to compare health promotion behavior, post-traumatic growth and quality of life according to the stages of survivorship in patients with female genital neoplasm. Methods: Data were collected from August 1st, 2011 to September 31st 2011 from 142 gynecologic cancer patients who completed treatment or were treated at an out-patient clinic. The instrument were HPLP developed by Walker, Sechrist & Pender, PTGI developed by Tedeschi & Calhoun, and Korean C-QOL. Results: Health promotion behavior scores were significantly higher in the acute survival stage than the extended survival stage. Post-traumatic growth score was higher in the acute survival stage than the extended survival stage. The quality of life scores were higher in the lasting survival stage than the extended survival stage. Conclusion: Gynecological cancer patients in the extended survival stage reported low scores of health promotion behavior, post-traumatic growth and quality of life. Intervention needed to be developed to improve health promotion behavior, post-traumatic growth and quality of life for patients with female genital neoplasm in the extended survival stage.

• Fisheries and Aquatic Sciences
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• 제21권2호
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• pp.4.1-4.5
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• 2018

Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.

• 한국양식학회지
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• 제12권4호
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• pp.255-266
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• 1999

The early development, growth, and morphological changes of Tiger puffer Takifugu rubripes are described on the basis of a seres of a series of reared specimens. Detailed o수 the early developmental stages are illusrtated withe special reference to morphological transformation. Egg and sperm of puffer fish Takifugu rubripes were obtained from mature adults under natural conditions, ferilized artificially and incubated in the laboratory. The incubation period of fertilizd eggs was 160 to 180 hours at a temperature of $15.1~ 18.0^{\circ}C$. Larvae were fed successively with rotifer, Artemia nauplii and artificial food for 90 days. The mean total length of newly-hatched larvae was about 2.8mm. Mouth opening occurred on the 1- day yolk-sac larvae and initial feeding was observed on th 4-5 days after hatching . The morphological transitions from the larvae to juvenile and juvenile and juvenile to young stages occurred when fish reached about 10mm in total length(about 30 days after hatching) and about 32mm in total length(about 60days after hatching) , respectively. The coefficient of variation in total length distribution increased with growth. Following the appearance of its peak at the size of about 18mm in total length, the coefficient value declined. Many changes in proportion of the body parts to total length were observed at about 9~10mm and 30~33mm in total length, corresponding to the transformations from larvae to juvenile and from juvenile to young, respectively. Also one big morphological change was observed at about 18mm in total length that divide the juvenile stage into two sub-stages.

• 대한원격탐사학회지
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• 제37권4호
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• pp.733-745
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• 2021

Although waxy corn varieties developed after the 1980s show differences depending on development stages and conditions, studies on the characteristics of waxy corn during the growth stage are rare. The subject of this study was a field survey and unmanned aerial vehicle (UAV) image acquisition of four waxy corn varieties cultivated in Idam-ri, Gammul-myeon, Goesan-gun, Korea. The study was conducted in four stages at intervals of two weeks after planting in 2019. The growth characteristics of each of the four varieties were analyzed using growth curves obtained based on field survey and UAV imagery data. The characteristics of each growth stage of the four varieties of corn, as assessed using normalized difference vegetation index (NDVI) and plant height (P.H.) values, were as follows. The growth model was identified as a model in which three-parameter logistic (3PL) curves reflect the growth characteristics of corn well. In particular, it was found that the variations in growth rate shown by P.H. and NDVI values clearly explain the differences between corn varieties. Among the four cultivars, growth and development first occurred at the early vegetative stage in Daehakchal, followed by Mibaek 2, Miheukchal, and finally Hwanggeummatchal. The variationsin P.H. and NDVI were achieved quickly and earlier in Daehakchal, followed by Mibaek 2, Hwanggeummatchal, and Miheukchal. It was confirmed that these results reflected the characteristics of the fast white-type varieties, while the black-type varieties were delayed, as in a previous study. These results reflect the resistance to lodging that affects the cultivation environment and the response characteristics to nutrients and moisture. It was confirmed that UAV accurately provides growth information that is very useful for analyzing the growth characteristics of each corn variety.

• 한국암석학회:학술대회논문집
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• 한국암석학회 1999년도 춘계학술대회
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• pp.70-74
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• 1999

• 한국전기화학회:학술대회논문집
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• 한국전기화학회 1999년도 제2회 총회 및 춘계학술발표회
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• pp.9-9
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• 1999

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.342-343
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• 2000

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.239-239
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 1994년도 추계 학술대회지
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• pp.70-71
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• 1994