• Title/Summary/Keyword: Growth reduction

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Resazurin Reduction Time Test to Determine Post-pasteurization Contamination and Shelf Life of Market Milk (시유의 2차오염과 저장가능기간을 결정하기 위한 Resazurin 환원시간검사)

  • Choi, S,H.;Choi, J.J.;Lee, S.B.;Yoon, Y.H.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.999-1006
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    • 2004
  • The selective media including NPC agar, DHL agar, MacConkey agar, and Cetrimide desoxycholate agar were compared to determine selectivity for the growth of bacteria Cetrimide desoxycholate agar was better than NPC agar, DHL agar, and MacConkey agar for the growth of psychrotrophic grarn-negatve bacteria including Pseudomonas. and for the inhibition of gram positive bacteria The specificity of resazurin reduction time test was investigated to determine post-pasteurization contamination of market milk. Equal volume of Cetrimide desoxycholate broth was added to market milk, which was then incubated at $21^{\circ}C$ for 18 hours. The growth of bacteria in the incubated milk was detected in resazurin reduction time test. The results in resazurin reduction time test and total bacteria number count of market milk after storage at $7^{\circ}C$ were relatively correlated each other. Pseudomonas was isolated most frequently from the market milk stored at $7^{\circ}C$ for 10 days, and Acinetobacter and Aeromonas followed. Acinetobacter, Pseudomonas and Enterobacter were frequently isolated from the mixture of market milk and Cetrimide desoxycholate broth incubated at $21^{\circ}C$ for 18hours in resazurin reduction time test.

Growth Properties of the Iron-reducing Bacteria, Shewanella putrefaciens IR-1 and MR-1 Coupling to Reduction of Fe(III) to Fe(II)

  • Park, Doo-Hyun;Kim, Byung-Hong
    • Journal of Microbiology
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    • v.39 no.4
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    • pp.273-278
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    • 2001
  • Shewanela, putrefaciene IR-1 and MR-1 were cultivated by using various combinations electron donor-acceptor, lactate-Fe(III) lactate-nitrate, pyruvate-FE(III), pyruvate-nitrate H$_2$ acetate-Fe(III) and H$_2$-acetate-nitrate. Both strains grew fermentatively on pyruvate and lactate but not on without and electron acceptor. In culture with Fe(III), both astrains grew on pyruvate and lactate but on H$_2$-acetate- CO$_2$. In cultivation with nitrate, both stains grew on pyruvate lactage and on H$_2$-acetate-CO$_2$ The growth yields of IR-1 pyruvate, pyruvate-Fe(III) and lactate-Fe(III) were about 3.4, 3.5, and 3.6(g cell/M substrate), respectively. From the growth properties of both strains on media with Fe(III) as an electron acceptor, the bacterial growth was confirmed not to be increased by addition of Fee(III) as an electron acceptor to the growth medium, which indicates a possibility that the dissimilatory reduction of Fe(III) to Fe(III) may not be coupled to free energy production.

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A Countermeasure of New Protectionism by Green Growth in Korea (녹색성장과 보호무역주의에 따른 대응 방안)

  • Lee, Woo-Chae;Yoon, Young-Han
    • International Commerce and Information Review
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    • v.11 no.4
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    • pp.395-418
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    • 2009
  • Appeared in the 1970s he started the global warming problem of carbon reduction, unlike the expected withdrawal of the United States was anticipated to be nominal stripping. However, EU mainly carbon market, buy and sell rights to be disposed of evolution was born. In fact, the current reduction of the Kyoto Protocol, and Korea will be designated as the bureau had been scheduled. However, due to economic recession in Korea's status as exempt countries have enjoyed a considerable period. Global warming is a problem in earnest, however, the United States to participate in one or more such discussions since 2013, clearly this will begin the international order. And international order that is more stringent than expected, and is expected to be strong. Then, however, an interesting thing to justify protection of the environment in some countries to protect domestic industry, the movement is visible is the side. In fact the WTO since the conclusion of all non-tariff trade barriers were abolished. However, recently, New Round of the delay based on reciprocity and fair trade in the framework of environmental protection to justify the movement to protect the domestic industry has been captured. These trends are not friendly, never in Korea. The rationale is that these regulations are enforced, many of our countries and countries with significant trade transactions and the enforcement points. And South Korea's automotive, semiconductors, ships, etc. The main products are much discussed in the international regulations are being referred to as the target point is due. Korean government to actively participate in the international situation and efforts to explore new markets, said yesterday, and 'Low Carbon, Green Growth' was declared. And the Intergovernmental Panel on Climate Change(IPCC) recommendations of the best 30% reduction target was present. This is nothing different about this objection is true. A more fundamental solution to faithfully perform the reduction targets, while the development of environmentally friendly products and the incidence of international standards through the development and expansion of new growth engines, Indeed we are expected to be a fundamental methodology.

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The Analysis of Sedum Suitable for the Effect of Modulability the Temperature of the Rooftop Greening (옥상녹화의 온도조절효과에 적합한 세덤류 분석)

  • Lee, Bitnara;Kim, Ye-Seul;Lee, Eun-Heui
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.2
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    • pp.53-64
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    • 2015
  • Rooftop greening is the alternative of urban heat island. The purpose of this study is selecting Sedum to the effect of temperature reduction of the rooftop greening. Since the state of growth is excellent, Sedum kamtschaticum, Sedum takesimense and Sedum middendorffianum surveyed as coverage. It was investigated that there is the effect of reducing the temperature. The effect of temperature reduction of Sedum counted compound was found to be associated with state of growth. When you construct a rooftop greening, planting Sedum kamtschaticum, Sedum takesimense and Sedum middendorffianum is helpful to the effect of temperature reduction. Also, the ingredients various types of Sedum in order to reduce the effective temperature, it must be densified. It must demonstrate an additional effect of temperature reduction of Sedum through complementary and continuous monitoring of the future temperature monitoring method.

Reduction of Hexavalent Chromium by Escherichia coli ATCC 33456 in Batch and Continuous Cultures

  • Bae, Woo-Chul;Kang, Tae-Gu;Kang, In-Kyong;Won, You-Jung;Jeong, Byeong-Chul
    • Journal of Microbiology
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    • v.38 no.1
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    • pp.36-39
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    • 2000
  • Toxic hexavalent chromium, Cr(VI), was reduced to a less toxic trivalent chromium form by E. coli ATCC 33456. The suitable electron donor for Cr(VI) reduction was glucose. E. coli ATCC 33456 was more resistant to metal cations than other reported Cr(VI) reducing microorganisms. Cell growth was inhibited by the presence of Cr(VI) in a liquid medium and Cr(VI) reduction accompanied cell growth. With a hydraulic retention time of 20 h, Cr(VI) reducing efficiency was 100% to 84% when Cr(VI) concentration in the influent was in the range of 10 to 40 mg L$\^$-1/. Specific rate of Cr(VI) reduction was 2.41 mg Cr(VI) g DCW$\^$-1/ h$\^$-1/ when 40 mg L$\^$-1/ of Cr(VI) influent was used. This result suggested the potential application of E. coli ATCC 33456 for the detoxification of Cr(VI) in Cr(VI) contaminated wastewater.

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Changes of Tree Growth and Fruit Quality of "Yumi" Peach under Long-Term Soil Water Deficit

  • Yun, Seok Kyu;Kim, Sung Jong;Nam, Eun Young;Kwon, Jung Hyun;Chung, Kyeong Ho;Choi, In Myung;Kim, Ghiseok;Shin, Hyunsuk
    • Journal of Biosystems Engineering
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    • v.42 no.4
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    • pp.276-282
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    • 2017
  • Purpose: This paper presents the effects of soil drought stress during the growing season and pre-harvest period on tree growth and fruit quality of "Yumi" peach, an early season cultivar. Methods: Soil drought stresses were treated with four levels of -30, -50, -60, and -70 kPa during long term (LT) and short term (ST). For LT treatments, soil water was controlled for nine weeks from May 1 to July 5, which was assumed as the full growing season. For ST treatments, soil water was controlled for four weeks from June 10 to July 5, which was assumed as the pre-harvest season. Tree growth and leaf photosynthesis were measured, and fruit characteristics such as fruit weight and diameter, soluble solid and tannin contents, and harvest date were investigated. Results: Soil water deficit treatments caused a significant reduction in tree growth, leaf photosynthesis, and fruit enlargement. LT water stress over -60 kPa during the full growing season caused significant reduction in tree growth, including shoot length, trunk girth, leaf photosynthesis, and fruit enlargement. ST water stress over -60 kPa during the pre-harvest period also induced significant reduction in leaf photosynthesis and fruit enlargement, while tree growth was not reduced. In terms of fruit quality, water stress over -50 kPa significantly reduced fruit weight, increased soluble solid and tannin contents, and delayed harvest time in both LT and ST treatments. Conclusions: As a result, it is assumed that LT water stress over -60 kPa can reduce both tree growth and fruit enlargement, whereas ST water stress over -50 kPa can reduce fruit enlargement without reducing tree growth. From an agricultural perspective, moderate water deficit like -50 kPa treatments could have positive effects, such increased fruit soluble solid contents along with minimal reduction in fruit size.

Effects of Initial Inoculum Size, Liquid Volume and Medium Feeding Strategy on Panax ginseng Hairy Roots Growth

  • Jeong, Gwi-Taek;Park, Don-Hui;Hwang, Baek
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.250-253
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    • 2000
  • We researched effects of growth on initial inoculum size, liquid volume, and medium feeding rate etc. Cell suspension inoculated at low cell concentrations showed a typical growth reduction, whereas root cultures showed an improvement in growth. In this paper, Hairy roots showed high growth rate at 0.4 % inoculum size and 100 mL liquid volume in 250 mL flask cultures.

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Hydrogenotrophic Sulfate Reduction in a Gas-Lift Bioreactor Operated at $9^{\circ}C$

  • Nevatalo, Laura M.;Bijmans, Martijn F. M.;Lens, Piet N. L.;Kaksonen, Anna H.;Puhakka, Jaakko A.
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.615-621
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    • 2010
  • The viability of low-temperature sulfate reduction with hydrogen as electron donor was studied with a bench-scale gas-lift bioreactor (GLB) operated at $9^{\circ}C$. Prior to the GLB experiment, the temperature range of sulfate reduction of the inoculum was assayed. The results of the temperature gradient assay indicated that the inoculum was a psychrotolerant mesophilic enrichment culture that had an optimal temperature for sulfate reduction of $31^{\circ}C$, and minimum and maximum temperatures of $7^{\circ}C$ and $41^{\circ}C$, respectively. In the GLB experiment at $9^{\circ}C$, a sulfate reduction rate of 500-600 mg $l^{-1}d^{-1}$, corresponding to a specific activity of 173 mg ${SO_4}^{2-}g\;VSS^{-1}d^{-1}$, was obtained. The electron flow from the consumed $H_2$-gas to sulfate reduction varied between 27% and 52%, whereas the electron flow to acetate production decreased steadily from 15% to 5%. No methane was produced. Acetate was produced from $CO_2$ and $H_2$ by homoacetogenic bacteria. Acetate supported the growth of some heterotrophic sulfate-reducing bacteria. The sulfate reduction rate in the GLB was limited by the slow biomass growth rate at $9^{\circ}C$ and low biomass retention in the reactor. Nevertheless, this study demonstrated the potential sulfate reduction rate of psychrotolerant sulfate-reducing mesophiles at suboptimal temperature.

A Study on Reduction of Food Waste (음식물 쓰레기 소멸화에 관한 연구)

  • 서명교;이상봉;이국의;이상훈
    • Journal of Environmental Health Sciences
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    • v.27 no.1
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    • pp.14-19
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    • 2001
  • The physical and chemical transformation and reduction degree of food waste were investigated in a food waste reduction machine using thermophilic bacteria. The first operation of the reduction machine for grain, vegetables, fishes and flesh wastes proceeded during three weeks. The first and second reduction percentages of the wastes were 98.3% and 93.2%, respectively. The residue of food waste was composed of fruits, fish, and vegetables. The temperature distribution of the reduction machine ranged between 30 and 6$0^{\circ}C$ appropriate for growth of thermophilic bacteria. At initial stage the pH in the reduction machine decreased with organic acids produced, but increased as the organic acids decomposed by different thermophilic bacteria. In the reduction machine, the moisture content of the food waste was reduced from 80-90% to 10-20% after 24 hours, and the salinity of residue was 0.29% after the second operation. The degree of odor was most high between 2 and 4 hours.

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