• Journal of Pharmacopuncture
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• v.13 no.1
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• pp.5-14
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• 2010

Objectives : This study was performed to examine the anticancer effect of mountain ginseng Pharmacopuncture(MGP) to the nude mouse of lung carcinoma induced by NCI-H460 human nonsmall lung cancer cells. Methods : Human lung cancer (NCI-H460) cells were cultured and applied to evaluate anti-tumor activity in nude mice. After confirmed tumor growth in mice, MGP was treated per 0.1ml/kg dose to intraperitoneal and intravenous injection everyday for four weeks. And checked the changes in body weights, tumor volume, mean survival time and percent, increase in life span, histo-pathological findings, organ weights, and blood chemistry levels. Results : The results of in vivo study showed that MGP may have potential as growth inhibitor of solid tumor induced NCI-H460 without marked side effects. MGP inhibited dosage-dependently the growth of NCI-H460 cell-transplanted solid tumor compared with the control group. And mean survival time of MGP treated group was prolonged comparing with control group. Generally the group of intravenous injection is more effective than intraperitoneal injection. Conclusion : These results were suggested that MGP may be a useful anticancer agent for therapy of human lung cancer. And follow study need for the certain evidence.

• Journal of Ecology and Environment
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• v.29 no.2
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• pp.151-155
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• 2006

Root nodule biomass, and seedling biomass and growth were examined for 2-year-old Robinia pseudoacacia and Amorpha fruticosa seedlings following fertilization treatments. Organic fertilizer, solid combination fertilizer, and organic fertilizer plus solid combination fertilizer were used for the study. Root nodule biomass (g/plant) ranged from 3.00 to 7.06 for R. pseudoacacia and varied from 1.52 to 2.32 for A. fruticosa, respectively. In all treatments, root nodule biomass of R. pseudoacacia was significantly higher than those of A. fruticosa. Fertilization significantly increased root nodule biomass for only R. pseudoacacia, however, there were no significant differences in root nodule biomass among fertilization treatments. Root nodule biomass was not influenced by soil nitrogen (N) and phosphorous (P) concentrations following fertilization treatments. Seedling biomass (components and total) and growth (diameter at root collar and height) were strongly correlated with root nodule biomass for the two N fixing tree species.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.223-227
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• 1995

Immature flowers and lateral buds of Neoregeria carorinae cv. Tricolor were cultured for micropropagation and the collecting times of materials, growth regulators and theirs concentrations, and cultural methods on the formation of adventitious buds and growth were investigated in this experiment The formation rate was the highest in immature flowers collected at 4weeks after flower bud differentiation and in buds at 7weeks after flower differentiation of adventitious buds. MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L BA was the most favorable for the formation of adventitious buds. Solid medium was more effective for the formation of adventitious buds than liquid one. MS medium with 1.0 mg/L NAA was the most suitable for the rooting of regenerated shoot. Liquid medium was effective for the rooting of regenerated shoot than solid one.

• Analytical Science and Technology
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• v.17 no.5
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• pp.388-392
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• 2004

Structural studies of membrane proteins, importantly involving interpretation of genomics information, many signaling pathway and major drug target for drug discovery, are having difficulty in characterizing the function using conventional solution nmr spectroscopy and x-ray crystallography because phospholipid bilayers hindered fast tumbling and crystallization. Here, we studied the structure of the pf1 coat protein in oriented phospholipid bilayers by home-built solid-state NMR probe. Bacteriophage pf1 was purified from Paeudomonas Aeruginosa and coat protein of bacteriophage pf1 was isolated from DNA and other proteins.

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.31-35
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• 1996

To develope a solid culture media for the mass culture of Beauveria bassiana conida, wheat bran was selected as C source and N source. The pellet media were prepared without(P-I) and with(P-II) solidified ingredient. The moisture for the growth of B. bassiana was required over 4 : 2 of Media : D.W (W/V). The growth of Beauveria bassiana on the media type was more effective in media of pellet type than that of powder type. In addition, production of Beauveria bassiana conidia on the media size was more effective in media of S type($\Phi$3mm) than that of L type($\phi$7mm). And the yield of Beauveria bassiana conidia at 2$0^{\circ}C$ cultivation on 3 weeks post inoculation was similar to that of $25^{\circ}C$. Above-mentioned results showed that pellet medium (P-I) was effective to growth of Beauveria, suggesting that the pellet media may be useful in the mass production of Beauveria bassiana conidia.

• Korean Journal of Environmental Agriculture
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• v.42 no.3
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• pp.177-183
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• 2023

Rendering, is attracting attention as a technology that can stably and quickly process livestock carcasses. However, large amounts of livestock carcass solid residues are discharged in this process and limited methods are available for recycling them. In this study, rendered animal carcass solid residues were pyrolyzed to produce carbonized materials (350℃; RACR-C) and their chemical properties were investigated. Further, RACR-C were applied to cabbage cultivation for investigating their crop growth characteristics and soil improvement effects. RACR-C contained large amounts of fertilizer components such as nitrogen and phosphorus, and showed no toxic effects on the seedling growth of crops. The content of water-soluble nutrients released from RACR-C under the reaction time increased rapidly within 30 min, but was insignificant compared to the total content. Thus, most fertilizer components in RACR-C were not readily soluble in water. The optimal application amount for applying RACR-C to cabbage cultivation based on the changes in cabbage growth, inorganic content, and soil chemistry was 200 kg/10a. Overall, pyrolysis of solid residues after rendering livestock carcass to produce carbonized material as a soil improver is an effective method to recycle the waste discharged from the rendering process.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.62-67
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• 2001

This study was carried out to investigate the optimum composition and concentration of medium for the acclimation of the shoots derived from bioreactor culture of Scrophularia buergeriana. Rooting and growth of the shoots cultured in bioreactor for 4 weeks were better on liquid full strength MS medium while one-tenth of MS medium was good for rooting and growth of the shoots cultured in bioreactor for 6 weeks. Leaf expansion was more effective in the shoots cultured for 6 weeks on solid MS medium than in those cultured for 4 weeks. No leaf expanded in liquid medium. Vitrification rate was higher in full strength solid MS medium than in half strength MS medium. It is concluded that acclimation of the shoots is more effective on the half strength solid MS medium containing 1.2% agar.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3909-3919
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• 2013

The aim of the present investigation was to evaluate the effect of A ferruginea extract on Dalton's lymphoma ascites (DLA) induced tumours in BALB/c mice. Experimental animals received A ferruginea extract (10 mg/kg.b.wt) intraperitoneally for 14 consecutive days after DLA tumor challenge. Treatment with extract significantly increased the life span, total white blood cell (WBC) count and haemoglobin (Hb) content and decreased the level of serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (${\gamma}$-GT) and nitric oxide (NO) in DLA bearing ascites tumor models. In addition, administration of extract significantly decreased the tumour volume and body weight in a DLA bearing solid tumor model. The levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-1 beta (IL-$1{\beta}$), interleukin-6 (IL-6) and granulocyte monocyte-colony stimulating factor (GM-CSF), as well as pro-angiogenic growth factors such as vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) were elevated in solid tumour controls, but significantly reduced by A ferruginea administration. On the other hand, the extract stimulated the production of interleukin-2 (IL-2) and interferon-gamma (IFN-${\gamma}$) in animals with DLA induced solid tumours. Increase in $CD4^+$ T-cell population suggested strong immunostimulant activity for this extract. GC/MS and LC/MS analysis showed quinone, quinoline, imidazolidine, pyrrolidine, cyclopentenone, thiazole, pyrazole, catechin and coumarin derivatives as major compounds present in the A ferruginea methanolic extract. Thus, the outcome of the present study suggests that A ferruginea extract has immunomodulatory and tumor inhibitory activities and has the potential to be developed as a natural anticancer agent.

• Journal of the Korean Magnetics Society
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• v.21 no.3
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• pp.108-115
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• 2011

Principles in the synthesis of small-sized high-quality crystals for the experimental condensed matter physics will be discussed in this paper. Synthesis process and cautions will be introduced especially for the synthesis methods which can be easily accessible to researchers. Starting from the solid state reaction which is the most common synthesis method, I will explain the quartz tube sealing that is crucial for making polycrystalline materials as well as single crystals in various conditions. Finally, basics of single crystal growth and various techniques will be introduced on the whole for the researchers who are not familiar with the material synthesis.

• KSBB Journal
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• v.4 no.1
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• pp.18-20
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• 1989

Solid gelatin microcarrier with the size distribution between $100{\mu}{\textrm}{m}$ and $400{\mu}{\textrm}{m}$ was prepared for the attachment and growth experiment for anchorage-dependent animal cell lines, i.e., L 929 and BHK 21. The growth and the maximum cell densities on this gelatin based and polyacrylamide (PAA) microcarriers were compared with those on the commercial dextran based Cytodex 3 microcarrier. Both cell lines showed good comparable attachment and growth patterns on the above three microcarriers. The mouse fibroblast, L 929 showed about the same maximum cell density on PAA, gelatin and Cytodex 3 MC'S BUT BHK 21, the baby hamster kidney cell line, showed the best result on Cytodex 3, which was about $4\times10^6$ cells/ml with the microcarrier concentration of 10 g/1.