• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.16-28
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• 2023

Lung adenocarcinoma accounts for about 40% of all lung cancers. With the recent development of gene profiling technology, studies on mutations in oncogenes and tumor suppressor genes, which are important for the development and growth of tumors, have been actively conducted. Companion diagnosis using next-generation sequencing helps improve survival with targeted therapy. In this study, formalin-fixed paraffin-embedded tissues of non-small cell lung cancer patients were subjected to hematoxylin and eosin staining for detecting genetic mutations that induce lung adenocarcinoma in Koreans. Immunohistochemical staining was also performed to accurately classify lung adenocarcinoma tissues. Based on the results, next-generation sequencing was applied to analyze the types and patterns of genetic mutations, and the association with smoking was established as the most representative cause of lung cancer. Results of next-generation sequencing analysis confirmed the single nucleotide variations, copy number variations, and gene rearrangements. In order to validate the reliability of next-generation sequencing, we additionally performed the existing genetic testing methods (polymerase chain reaction-epidermal growth factor receptor, immunohistochemistry-anaplastic lymphoma kinase (D5F3), and fluorescence in situ hybridiation-receptor tyrosine kinase 1 tests) to confirm the concordance rates with the next-generation sequencing test results. This study demonstrates that next-generation sequencing of lung adenocarcinoma patients simultaneously identifies mutation.

• Journal of Aquaculture
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• v.14 no.3
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• pp.181-195
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• 2001

To stabilize the lantern cage culture system of Patinopecten yessoensis(Jay) in the eastern coast of Korean peninsula, optimum conditions such as time of transplantation, rearing density and depth, and time of harvest were identified. During the period from January 1991 to December 1998, the water temperature ranged from 4.7 to 21.4$^{\circ}C$ at 15-30 m depth and 4.9 to 25.7$^{\circ}C$ at the surface; these thermal ranges were within the optimal ranges (5-23$^{\circ}C$) prevailing at 15-30 m depth at surface water. Annual thermal changes indicated that the prevailing temperature during the years 1993 and 1996 was near optimum, but higher during the years 1994, 1997 and 1998, when mass mortality and growth retardation occurred. Salinity (32.0- 34.4$\textperthousand$) and dissolved oxygen (4.14 -8.11 $\mu\textrm{g}$/l) at 15 m depth were well within the optimum ranges. The chlorophyll concentrations (0.06 - 2.73$\mu\textrm{g}$/l) indicated that the study area was oligotrophic, although mass mortality did occur, when chlorophyll concentrations were high, especially in summer. Hence water temperatures and chlorophyll concentration are major factors related to survival and growth of the scallop. In terms of the shell height maximum growth occurred during spring (March-May; 8 - l3$^{\circ}C$) and fall (October-December; 11-l7$^{\circ}C$) in the lantern cage culture. Slow growth was recorded during late winter January-february; less than 7$^{\circ}C$) and mid-summer (August- September; more than 18$^{\circ}C$). Daily growth of shell height and total weight were 0.02∼0.24 mm and -0.07∼0.90 g at the rearing density of 12 individuals per net. Optimal .earing density in the lantern cage (ø50${\times}$20 cm) was 10∼15 individuals with the shell height of 5∼6 cm. The fastest growth rates were observed at 15∼20 m depth; however, it is recommended that 20∼30 m would be optimal. The scallops require 22 months to attain the commercial size of 10 cm shell height and 140 g total weigh, and are best harvested and sold during March-April.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.689-692
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• 2015

Background: Incorporation of molecular analysis of the epidermal growth factor receptor (EGFR) gene into routine clinical practice has shown great promise to provide personalized therapy of the non-small cell lung cancer (NSCLC) in the developed world. However, the genetic testing of EGFR mutations has not yet become routine clinical practice in territories remote from the central regions of Russia. Therefore, we aimed to study the frequency of major types of activating mutations of the EGFR gene in NSCLC patients residing in West Siberia. Materials and Methods: We examined EGFR mutations in exons 19 and 21 in 147 NSCLC patients (excluding squamous cell lung carcinomas) by real time polymerase chain reaction. Results: EGFR mutations were detected in 28 of the 147 (19%) patients. There were 19 (13%) cases with mutations in exon 19 and 9 cases (6%) in exon 21. Mutations were more frequently observed in women (42%, p=0.000) than in men (1%). A significantly higher incidence of EGFR mutations was observed in bronchioloalveolar carcinomas (28%, p=0.019) and in adenocarcinomas (21%, p=0.024) than in large cell carcinomas, mixed adenocarcinomas, and NOS (4%). The EGFR mutation rate was much higher in never-smokers than in smokers: 38% vs. 3% (p=0.000). The frequency of EGFR mutations in the Kemerovo and Tomsk regions was 19%. Conclusions: The incorporation of molecular analysis of the EGFR gene into routine clinical practice will allow clinicians to provide personalised therapy, resulting in a significant increase in survival rates and improvement in life quality of advanced NSCLC patients.

• Journal of Wetlands Research
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• v.20 no.2
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• pp.155-160
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• 2018

We investigated to know the growth patterns of Aedes albopictus larva at the four different temperature conditions. Each of 120 individuals was placed into a $20m{\ell}$ vial and 12 sets (a set of 10 vials) were separated into 12 water tanks ($17{\times}24{\times}18cm^3$). Each water tank was composed of 3 the $1^{st}$ instar, 3 the $2^{nd}$ instar, 2 the $3^{rd}$ instar, and 2 the $4^{th}$ instar. Three sets of water tanks were placed under the four different incubator temperatures ($17^{\circ}C$, $21^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$). We found that the eclosion rates were $20.00{\pm}5.77%$ at $21^{\circ}C$ and $3.33{\pm}3.33%$ at other temperatures. For the mosquito larva mortality rate, $1^{st}$ instar was $19.24{\pm}3.65%$, $2^{nd}$ instar was $16.48{\pm}3.25%$, $3^{rd}$ instar was $23.54{\pm}5.06%$, and $4^{th}$ instar was $40.74{\pm}7.08%$. The lowest mortality rate in growth stages according to temperature was $13.33{\pm}6.67%$ at $17^{\circ}C$ in $1^{st}$ instar larva, $7.41{\pm}7.41%$ at $21^{\circ}C$ at $2^{nd}$ instar larva, $10.74{\pm}6.43%$ at $24^{\circ}C$ in $3^{rd}$ instar larva, and $20.37{\pm}5.46%$ at $28^{\circ}C$ in $4^{th}$ instar larva. The survival period of mosquitoes in underwater were $26.33{\pm}0.67days$ at $17^{\circ}C$, $23.33{\pm}1.33days$ at $21^{\circ}C$, $20.00{\pm}2.52days$ at $24^{\circ}C$, and $11.67{\pm}1.20days$ at $28^{\circ}C$. From our results the most effective temperature to the normal growth of mosquito larva was $21^{\circ}C$, and the highest mortality rate was shown at the $4^{th}$ instar stage of larva growth. Our results would provide the basic data for the mosquito larva's growth pattern.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.52-56
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• 1985

For the fundamental studies of radiation breeding in edible marine algae, the biological effects on conchospores of Porphyra species by gamma-irradiation were examined. Two varieties, Keun-cham-gim (Porphyra tenera Kjell. form tamatsuensis Miura) and Saga No.5, were chosen for this study, and their conchospores after r-irradiation($5.0{\sim}20.0$ KR) were cultured for 50 days. The results obtained were summarized as follows. 1. Gamma-irradiation in less than the dose of 20KR did not affect germination of conchospores, and almost all spores grew into two cells germ in 24 hours after irradiation, but withering germs were gradually increased in number according to higher exposure within 5 days old culture. 2. High irradiation caused the induction of giant cells, abnormal useless growth of hold-fast, lumpish thalli and callus-like lumpy tissues. 3. The liberation of neutral spores from young germs and carpospores from mature thalli were observed on the frond exposed at $10{\sim}20$ KR irradiation. All spores were normal in division and its size. 4. The best irradiation effect on growth of Keun-cham-gim was observed at 10 KR dose, whose growth-rates were $140\%$ in wet weight and $108\%$ in mean frond area, but only $48\%$ was recorded in wet weight at 20 KR exposure. Saga No.5 were in contrast with Keun-cham-gim, and their most growth-rate was $400\%$ in wet weight ($258\%$ in frond area) at 10 KR irradiation and the worst was $20\%$ at the dose of 20 KR. 5. The withering phenomenon to death by treatment of gamma-ray presented substantial difference between two varieties. Survival rate compared with control in Keun-cham-gim was $70.7\%$ at 20 KR, but that in Saga No.5 recorded $47.0\%$ at same dose. 6. Synthesizing the results of high and low r-irradiation, it was suggested tat high r-irradiation in more than 5.0 KR inhibited conspicuously the growth of germs derived from conchospores, and about half of them withered at 15.0 KR dose or more.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.86-93
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• 2018

This present study was conducted to develop the domestic cultivation kit using water celery(Oenanthe Stolonifera DC.) seed. As the result of germination rates in 3 type inbred lines, the IT 232354 had the highest initial germination rate and final germination rate, and was selected as the inbred line to be used in the cultivation kit. The development of the domestic cultivation kit was carried out using the IT 232354 seeds. It was possible to cultivate up to 3 times harvest using the same root in this cultivation kit, though the growth decreased rapidly in the $4^{th}$ cultivation. As a result of investigating the effects of the type of nutrient solution on growth of water celery, the overall growth was the lowest in the nutrient solution for Oenanthe Stolonifera DC.(N.S.D.). The shoot growth was similar to that of nutrient solution for leaves and stem vegetables (N.S.L.S.) and amino acid fermentation by-product liquid (A.F.B.L.), however in the root growth, the N.S.L.S. was more effective than A.F.B.L. When it was harvested 4 times consecutively after 1 time of planting, the last survival ratio of A.F.B.L. was 100% while their ratios were 96.4% and 49.8% each for N.S.L.S. and N.S.D. For the growth by cultivation kit type, the hole type cultivation kit increased slightly compared to the 3 hole type cultivation kit in the $1^{st}$ and $2^{nd}$ harvest, but there was no difference in the $3^{rd}$ and $4^{th}$ harvest. Total yield of one cultivation kits showed the 3 hole type cultivation kit is much higher than the 2 hole type cultivation kit. According to the results of this experiment, it is possible to harvest three times by planting one times if it was cultivated using N.S.L.S. and A.F.B.L. in the 3 hole type cultivation kit.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.4
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• pp.457-468
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• 1995

In order to develop a more practical culture system from the present running seawater tank system, two experiments of environmental factors, growth, survival rate and rearing density of olive flounder (Paralichthys olivaceus) were carried out for two consecutive years. Two groups of fish in initially averaging 7.5cm of total length, and 3.4g of body weight (EXP. I) and 5.0cm and 1.8g (EXP. II) were reared in the semi-closed recirculating seawater system equipped with the rotating biological contactors with the commercial culture scale. The dissolved inorganic nitrogen concentrations is EXP. I ranged 0,247-0.512 ppm of $NH_4-N$ (0.010-0.043 ppm of$NO_2-N$, and 0.108-0.342 ppm of $NO_3-N$, and those in EXP. II were 0.091-0.715 ppm, 0.002-0.045 ppm, and 0.007-0.277 ppm, respectively. Daily feeding rates of the fish were $0.67-2.41\%$ in EXP. I and $0.69-2.22\%$ in EXP_ II, and teed efficiency were $34.8-59.8\%\;and\;40.5-88.4\%$ in EXP. I and II, respectively. The average total ten說h and body weight were 40.0-42.8cm and 695.0-852.69g after 340 days culture in EXP. I, and 36.7-39.7cm and 552.4-706.4 g after 365 days culture in EXP. II, respectively. Survival rates of the fish at the end of EXP. I and II were $92.0\%\;and\;96.0\%,$ respectively. The ratio to body surface area of non-ocular side in all fish to bottom area of rearing tank, so-called covering rate, was used as an indicator of rearing density. The highest cowering rate and weight density of fish per $m^2$ of rearing tank at the end of experimental period were 2.2 and 34.1kg in EXP. I, and 2.6 and 36.3kg in EXP. II, respectively. For the commercial culture of olive flounder, the semi-closed recirculating seawater system was found to be more effective than the running seawater tank system in aspect to the fish productivity and protection of marine environment.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.545-553
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• 2019

This study was conducted to determine whether the water in which nitrate accumulated during long-term fish culture in an aquaponics system without water exchange could be removed and reused as catfish-culturing water. The catfish (Silurus asotus) were cultured in the urban aquaculture system using BFT (Biofloc Technology) aquaculture and an aquaponics system (two rearing tanks, 3 tons each) without exchanging the rearing water. After 151 days (from March to August) of rearing, 2.8 g of fry had grown to an average weight of 171.3 g (total weight, 56.53 kg) and 235.5 g (total weight 71.1 kg), respectively. The overall survival rate was 65% in the urban aquaculture system. However, the survival rate was 77.7% before separation into the two tanks. The survival rates after the separation were 92.9% and 78.0%. In the early biofloc watermaking process, there was a high mortality rate. After water stabilization, the mortality rate decreased and some mortality occurred during the period when the total amount of suspended solids (TSS) increased. The results of monthly blood analysis of the catfish showed that the AST concentration was significantly higher in April. Blood ALT levels and triglycerides showed no difference in the rearing period and the glucose, cholesterol, and total protein levels were significantly higher in July. There was no difference in the other periods. The plants produced by the aquaponics system using catfish-rearing water were lettuce, basil, chard, and red chicory. These showed smooth growth and a total of 148.85 kg of plants were harvested in five months. It was possible to remove nitric acid from the aquaponics system and reuse it as catfish-rearing water. Maintaining proper plant quantity according to the capacity of the catfish showed that the combination of agricultural and aquatic products was possible.

• Journal of Mushroom
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• v.19 no.3
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• pp.210-215
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• 2021

For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

• Journal of Aquaculture
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• v.7 no.1
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• pp.21-39
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• 1994

In order to study the optimum environmental condition for larvae culture of the freshwater crab, Eriocheir japonicus, larvae from different growth stages and young crab were cultured under the 16 different conditions of $4\times4$ temperature-salinity combinations (4 different temperatures at 22, 24, 26, $28^{\circ}C$ with 10.5, 17.5, 24.5 and $31.5\%$ of salinity). The duration of metamorphosis, metamorphosis rate, the interval of moulting period, and survival rate were measured from each experimental group of larvae and young crab under the different conditions. The results indicated that the optimum conditions may be a $24.5\%o$ of salinity at water temperature at 22, 24, and $26^{\circ}C$. At $28^{\circ}C$ with $24.5\%o$), the duration of metamorphosis reduced somewhat, nevertheless metamorphosis and survival rate decreased a lot. And the lower the salinity showed the lower the metamorphosis and survival rates at $28^{\circ}C$.