• Title/Summary/Keyword: Growth and survival

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Selection of Salt Tolerant Rapeseed (Brassica napus L.) Mutant Lines Induced by Irradiation of Proton Ion Beams and Gamma Ray (양성자 및 감마선처리에 의한 유채 내염성 변이계통 선발)

  • Kim, Jun-Su;Eun, Jong-Seon;Han, Seung-Jin
    • Journal of Radiation Industry
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    • v.5 no.3
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    • pp.211-220
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    • 2011
  • This study was carried out to develop salt tolerant varities of rapeseed (Brassica napus L.) which can be grown in the high salty reclaimed land. The seeds of three varieties 'Naehan', 'Tammi', and 'Halla' were treated by proton ion beams and gamma rays with 0 to 2,000 Gy. For the selection of salt tolerant lines, emergence and survival rate, and growth characteristics of $M_2$ to $M_4$ generations were investigated in the Saemangeum reclaimed fields with the different salt concentrations. The lines with potential salt tolerance were selected in the $M_4$ generation and tested indoor for their growth characteristics. There was no significant changes in the soil pH for $M_4$ generation during growth period. However, soil EC was higher in early spring than sowing period (mid October). In $M_4$ generation test, the seeds of original and selected line showed high rates of emergence and survival, as determined one month after sowing. After wintering, however, the original varieties showed the significant reduction in the survival rate, while the selected lines showed a higher survival rate and good growth, leading to the completion of their life cycle. Consequently we selected 9 lines from $M_4$ generation with better performance in growth and yield. Soil EC was $2.8{\sim}4.3dS{\cdot}m^{-1}$ during $M_4$ generation growth period. The laboratory test of the lines selected from $M_4$ generation was made for their salt tolerance potential. The selected lines showed higher chlorophyll and proline contents than the original varieties. There was also no significant difference in the emergence rate of seed between the original and selected varieties. In 200 mM natural sea salt, the $N{\gamma}600-21-1-641$ line derived from 'Naehan' was the highest in growth rate, leaf chlorophyll and proline contents. $T{\gamma}800-20-2-461$ line derived from 'Tammi' didn't show significant difference in growth rate compared to original variety in 200 mM and withered in 250 mM like other lines as time passed. $H{\gamma}200-7-1-740$ line showed similar growth and chlorophyll content compared to its original variety.

Effects of Shading Treatments on Growth of Abies koreana Seedlings in High-Temperature and High Light Environments (차광막 처리가 고온 및 고광도 환경에서 구상나무(Abies koreana) 묘목의 생육에 미치는 영향 )

  • Jae-Hyun Park;Hyo-In Lim;Han-Na Seo;Yong-Han Yoon
    • Journal of Environmental Science International
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    • v.32 no.11
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    • pp.811-820
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    • 2023
  • This study investigated the protective effects of shade nets on Abies koreana seedlings subjected to high temperature and luminosity stress, which are pertinent for plant survival in climate change scenarios. This study, conducted at Konkuk University, compared the growth, survival, and soil conditions of 3-year-old specimens across natural, greenhouse, and shaded settingsfrom July to September 2022. Our findings demonstrated that shade nets significantly enhanced seedling survival by moderating soil temperature and moisture. This is particularly evident in high-temperature conditions, where shade nets mitigate stress on seedlings and safeguard them from excessive sunlight exposure. Proper net installation height and location are crucial for optimal temperature and humidity control, suggesting broader applicability for various species and offering strategies to combat the ecological impacts of climate change.

Survival Strategies of the Rotifer Brachionus rotundiformis for Coexisting with the Copepod Apocyclops borneoensis in Laboratory Culture

  • Jung, Min-Min
    • Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.57-62
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    • 2012
  • Interspecific relationship between a euryhaline rotifer Brachionus rotundiformis and a cyclopoid copepod Apocyclops borneoensis was investigated in the laboratory culture. In a mixed culture of B. rotundiformis and A. borneoensis, population growth of B. rotundiformis was suppressed from day 10, while growth in a monoculture population continuously increased throughout the experimental period. However, the population growth of A. borneoensis in the mixed culture did not markedly differ from that in a monoculture population. Suppression of B. rotundiformis growth coincided with a decrease in the numbers of both non-egg-bearing and egg-bearing females, and increasing resting egg formation. Growth of A. borneoensis was not affected by the presence of the rotifer. However, relative growth index of ovisac bearing females in the mixed culture was 1.62 times higher than that in the monoculture. Presence of the copepod did not greatly reduce the food available to the rotifer population. The rotifer B. rotundiformis responded in a unique way, to stresses such as physical damage (filtering by A. borneoensis) with the production of many resting eggs to increase its chances of survival.

Study on the Improvement Effect of the Seeds Release of Sea Cucumber, Stichopus Japonicus (돌기해삼(Stichopus japonicus)의 방류효과 향상 연구)

  • Kim, C.W.;Jeong, D.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.19 no.1
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    • pp.15-23
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    • 2017
  • Effects of artificial releases of sea cucumber around the marine ranching area in Jeollanam-do were showed very efficient by the released good seeds(total weight, 3.2g) in 'Wolhang', compared with those in 'Hwatae'. The survival rates were the higher in 'Wolhang' by 66.0% than in 'Hwatae' by 27.0%. The results indicates that the releasing effect of sea cucumber depended on the releas size. To analyze the productivity we investigated the growth and survival rates of sea cucumber according to the releasing area. Our result showed that the growth rate and survival rate was higher in 'Hwatae' than other areas. In 'Hwatae', the growth rate and survival rate were 6.2g and 32.1% respective. The results indicates that if the food supply is continued, although proceeding contamination the productivity of sea cucumber increased. Therefore, in order to enhancement and conservation of resource, bottom of fish cage should consider to the utilization.

Larval Survival Rate and Growth of Pandalus gracilis Using Two Temperature Conditions Reared in the Laboratory (한국산 남방도화새우, Pandalus gracilis유생의 두 온도 구간에서의 생존율과 성장)

  • 최정화;마채우
    • Journal of Aquaculture
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    • v.17 no.1
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    • pp.58-61
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    • 2004
  • Larvae of Pandalus gracilis were reared in the laboratory under controlled conditions (salinity, 32.5∼34.5; photo-periods, 12L: 12D). The experiments were designed to examine effects of temperature (10 and 2$0^{\circ}C$) on larval development, survival, intermolt period and larval growth. The higher survival rate was obtained at 2$0^{\circ}C$ than at 1$0^{\circ}C$ and larvae reached later stages at 2$0^{\circ}C$. Intermolt period increased significantly with increasing of instar number and was longer at 1$0^{\circ}C$ than at 2$0^{\circ}C$.

Aberrant DNA Methylation and Epigenetic Inactivation of hMSH2 Decrease Overall Survival of Acute Lymphoblastic Leukemia Patients via Modulating Cell Cycle and Apoptosis

  • Wang, Cai-Xia;Wang, Xiang;Liu, Hai-Bai;Zhou, Zhi-Heng
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.1
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    • pp.355-362
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    • 2014
  • Objective: Altered regulation of many transcription factors has been shown to play important roles in the development of leukemia. hMSH2 can modulate the activity of some important transcription factors and is known to be a regulator of hematopoietic differentiation. Herein, we investigated epigenetic regulation of hMSH2 and its influence on cell growth and overall survival of acute lymphoblastic leukemia (ALL) patients. Methods: hMSH2 promoter methylation status was assessed by COBRA and pyrosequencing in 60 ALL patients and 30 healthy volunteers. mRNA and protein expression levels of hMSH2, PCNA, CyclinD1, Bcl-2 and Bax were determined by real time PCR and Western blotting, respectively. The influence of hMSH2 on cell proliferation and survival was assessed in transient and stable expression systems. Results: mRNA and protein expression of hMSH2 and Bcl-2 was decreased, and that of PCNA, CyclinD1 and Bax was increased in ALL patients as compared to healthy volunteers (P<0.05). hMSH2 was inactivated in ALL patients through promoter hypermethylation. Furthermore, hMSH2 hypermethylation was found in relapsed ALL patients (85.7% of all cases). The median survival of patients with hMSH2 methylation was shorter than that of patients without hMSH2 methylation (log-rank test, P=0.0035). Over-expression of hMSH2 in cell lines resulted in a significant reduction in growth and induction of apoptosis. Conclusions: This study suggests that aberrant DNA methylation and epigenetic inactivation of hMSH2 play an important role in the development of ALL through altering cell growth and survival.

Cell Free EGFR mRNA Expression and Implications for Survival and Metastasis in Non-Small Cell Lung Cancer Cases

  • Masroor, Mirza;Mir, Rashid;Javid, Jamsheed;Prasant, Y;Imtiyaz, A;Mariyam, Z;Mohan, Anant;Ray, PC;Saxena, Alpana
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.15
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    • pp.6445-6449
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    • 2015
  • Background: NSCLC is a disease involving uncontrolled cell growth, which could result in metastases into nearby tissues beyond the lungs. Materials and Methods: The aim of the present study was to analyze the influence of epidermal growth factor receptor (EGFR) gene expression on metastasis and survival in NSCLC patients. The present case-control study included 100 cases of NSCLC patients and 100 age and sex matched controls. EGFR gene expression was analyzed by quantitative real time PCR using serum RNA. Association with NSCLC patient survival was analyzed by the Kaplan-Meier method. Results: We analyzed EGFR gene expression and observed mean increased gene expression of 13.5 fold in NSCLC patients. Values reflected overall survival of patients with a median of 15.8 months in the cases of <13 fold increased gene expression vs 6.7 months with >13 fold increased EGFR gene expression (p=0.005). Distant metastatic patients with <13 fold increased EGFR gene expression had 7.9 months of median survival time while>13 fold increased EGFR gene expression had only 5 months of median survival time (p=0.03). Non metastatic patients with <13 fold increased EGFR gene expression had 18 months of median survival time as compared to only 7.1 months with >13 fold increased expression. Conclusions: Higher cell free EGFR mRNA expression may play an important role in causing distant metastases and reducing overall survival of NSCLC patients in the Indian population.

Effect of Size Grading on Growth, Feed Efficiency and Survival in Olive Flounder (Paralichthys olivaceus) (동일연령군에서 크기 선별에 따른 넙치(Paralichthys olivaceus) 성장, 사료효율 및 생존율의 비교)

  • Kim, Jong-Hyun;Kim, Hyun-Chul;Lee, Jeong-Ho;Noh, Jae-Koo;Lee, Mi-Sug;Kim, Kyung-Kil
    • Journal of Aquaculture
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    • v.18 no.3
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    • pp.154-159
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    • 2005
  • This study was conducted to evaluate the effects of size grading on growth, feed efficiency and survival of juvenile olive flounder. Juvenile flounder were divided into four groups by initial average size; Small group $(1.3{\pm}0.23g)$, medium group $(3.1{\pm}0.45g)$, large group $(4.9{\pm}0.57g)$ and ungraded group $(3.3{\pm}1.66g)$. Triplicate groups of 100 fish were reared over 8 weeks. In final body weight distribution, frequency of the small size flounder (10 g) was markedly higher in the ungraded group than in the small group. Specific growth rate, feed efficiency and survival in the ungraded group were significantly lower (P<0.05) than those in the pooled data of the othor three graded groups, although feed intake in the ungraded group was significantly higher (P<0.05) than that of the pooled data of the other three graded groups. These results show that the small flounder gained significantly faster growth and higher survival in the absence of the large flounder. Therefore, size grading seems to be an important and necessary operation to improve the growth and survival of juvenile olive flounder (1-5 g).

Growth and Survival Rates of Flat Oyster, Ostrea denselamellosa, by Condition of Larval Cultivation (벗굴 (Ostrea denselamellosa) 유생의 생육조건에 따른 성장과 생존)

  • Yang, Moon-Ho;Oh, Bong-Se;Han, Chang-Hee
    • The Korean Journal of Malacology
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    • v.19 no.2
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    • pp.133-142
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    • 2003
  • For the effective seedling production of flat oyster, Ostrea denselamellosa, dietary value of live food, densities, water temperature and salinity on growth and survival rate of the larvae were examined. In rearing larvae by feeding them phytoplankton diets, the optimal survival rate and growth rate of larvae were found using a mixed phytoplankton diet which was mixed with Isochrysis galbana, Chaetoceros calcitrans and Chlorella sp. The highest growth and survival rates of the larvae were 208.4% and 38.8% with the phytoplankton diet. In growth and survival rates of larvae with various rearing densities, the highest survival and growth rates were 228.1% and 29.0% at the density of 2 individuals/ml. In observing rearing experiments of the flat oyster larvae under various temperature conditions, average growth rates of the larvae in respect to shell length were 202.2%, 240.4%, 250.6% and 121.3% in natural water temperatures (18-22$^{\circ}C$), 24$^{\circ}C$, 28$^{\circ}C$ and 32 $^{\circ}C$, respectively. And average survival rates of the larvae were 16.0%, 32.0%, 13.0% and 0% in natural water temperatures (18-22$^{\circ}C$), 24$^{\circ}C$, 28$^{\circ}C$ and 32$^{\circ}C$, respectively. In rearing at various salinities, the highest growth rates of the larvae in shell length was 240.0% at 30.0 psu and the highest survival rate was 31.0% at 25 psu.

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Influence of Temperature on the Survival, Growth and Sensitivity of Benthic Amphipods, Mandibulophoxus mai and Monocorophium acherusicum (국내산저서단각류 Mandibulophoxus mai와 Monocorophium acherusicum의 생존, 성장 및 민감도에 대한 온도의 영향)

  • Lee Kyu-Tae;Lee Jung-Suk;Kim Dong-Hoon;Kim Chan-Kook;Park Kun-Ho;Kang Seong-Gil;Park Gyung-Soo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.8 no.1
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    • pp.9-16
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    • 2005
  • A series of experiments was conducted to evaluate the effects of temperature on the survival, growth and sensitivity of the benthic amphipods, Mandibuluphoxus mai and Monocnrophium acherusicum, which have been recently developed as new sediment toxicity testing species in Korea. The biological performance for each amphipod species was determined by the survival and growth rates at different water temperatures. The influence of temperature on the sensitivity to reference toxicant, Cd, was determined by the comparison of survival rates of amphipods exposed to control and Cd-spiked seawater at different temperatures. Temperature significantly influenced on the survival, growth and Cd sensitivity of both amphipods. Tolerable ranges of temperature for the >80% individuals of both M. mai and M. acherusicum with sediment substrates were mostly overlapped (13 to 22℃). The daily growth rates of M. mai and M. acherkisicum increased proportionally with temperature up to 20℃ and 25℃. respectively. Similarly, the sensitivities of M. mai and M. acheyusicum to Cd increased with temperature up to 20℃ and 15℃, respectively. Overall results showed that temperature is a substantially important factor potentially influencing the results of lethal and sublethal bioassays using the amphipods. Therefore, defining the adequate ranges of experimental temperature for the toxicity testing species is the pre-requisite for the development of standardized bioassay protocols.

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