• Title/Summary/Keyword: Growth Medium

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Effects of Low Dose $\gamma$ Radiation on Callus Growth of Lithospermum erythrorhizon S. (지치 (Lithospermum erythrorhizon S.) Callus 생장에 미치는 저선량 $\gamma$선의 효과)

  • 황혜연;김재성;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.305-310
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    • 2001
  • The effect of low dose ${\gamma}$-radiation on the callus growth of Lithospermum erythrorhizon S. cultured on different medium and lighting condition was investigated. The 8 Gy irradiation stimulated callus growth on LS medium supplemented with BA 2 mg/L and NAA 2 mg/L, however, the growth of callus was more effective on LS medium supplemented with BA 1 mg/L and NAA 1 mg/L under 16 hrs day light. And on the LS medium containing IAA 0.2 mg/L, 16 Gy irradiation increased the callus growth by supplement with kinetin 2 mg/1 and the effect of kinetin was higher than BA at same concentration. The growth of callus was more vigorous on LS medium than MS medium in general. On M-9 medium, the growth of callus was poor regardless lighting conditions, however, by ${\gamma}$-ray irradiation of 16 Gy or 30 Gy, callus growth rates were increased by 30% or more than 30%, especially, under 16 hrs day light condition.

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Effect of Waste Tire Chips on the Growth and Nutrient Content of Cymbidium Pine Clash 'Moon Venus' (심비디움 Pine Clash 'Moon Venus'의 생장 및 양분함량에 미치는 폐타이어칩의 영향)

  • Kim, Hong-Yul
    • Journal of agriculture & life science
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    • v.43 no.2
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    • pp.17-21
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    • 2009
  • This experiment was conducted to clarify the effect of waste tire chip on the growth and nutrient content of Cymbidium Pine Clash 'Moon Venus'. There were no significant differences between bark only medium and mixed medium in leaf and stem growth. But in both medium and large size chip only, the leaf and stem growth decreased remarkably. The total number of roots, new roots and root length had similar tendency as in leaf and stem growth. In medium and large tire chip only, the decayed roots increased. There were no significant differences between bark only medium and mixed medium in total sugar, starch, content of chrolophyll, N, P and K, but decreased significantly in both medium and large size chip only.

Effect of Commercial Organic :Medium Amended with Vermicast on the Growth of Rice Seedlings(Oryza sativa L.) -Amended with Vermicast of Oyster Mushroom Waste- (지렁이 분립을 첨가한 유기상토가 벼의 유식물체 생육에 미치는 영향 -느타리버섯 폐배지로 생산한 분립의 첨가-)

  • Lee, Ju-Sam;Kim, In-Soo
    • Korean Journal of Organic Agriculture
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    • v.17 no.1
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    • pp.55-62
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    • 2009
  • In this paper, we assessed the growth of rice seedlings(Chucheong variety) in commercial organic growth medium that was substituted with different ratios of vermicast of oyster mushroom waste grown under potting alone, and potting and floor layering treatment. The commercial organic growth medium was substituted with vermicast at ratios of control, 2%, 4% and 6%, respectively. The control consisted of commercial organic growth medium alone without vermicast. Incorporation of $4%{\sim}6%$ vermicast of oyster mushroom waste into a commercial organic growth medium enhanced the growth of rice seedlings significantly as compared to commercial organic growth medium alone(control), and 2% amended with vermicast in potting treatment. This results demonstrate that substitution with low ratios of vermicast($4%{\sim}6%$) will promote growth of rice seedlings. The growth of rice seedlings in commercial organic growth medium alone without vermicast was enhanced significantly as compared to the substituted with vermicast in floor layering treatment, it may due to nutrient uptake by elongated root from the vermicast when applied to on the floor layering. Floor layering treatment is an effective method for potting processing of vermicast. The vermicast of oyster mushroom waste should have a high safety and great potential as materials of growth media for increasing plant growth, either as soil conditioner, or as substitution or amendments to commercial organic growth medium. For the enhanced growth of rice seedlings, demand to increase with total nitrogen, and decrease with the carbon and nitrogen ratio(C/N) of commercial organic growth medium supplied by such as vermicast.

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Microcarrier Culture of an Anchorage-dependent Cell Using Cytodex-3 (Cytodex-3를 이용한 부착성 동물세포의 미립담체 배양)

  • 김정회;최준호;웨이슈후
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.231-235
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    • 1989
  • Possibility of using microcarriers for the growth of a transformed human embryonic kidney cell line 293 was investigated. The cell grew well in a static culture such as T-flasks with medium of DME/F12 (3:1) mixture supplemented with 5% FBS, but it was most difficult to make the cells grow on microcarriers mainly due to the low attachment efficiency and poor spreading at initial stage of the culture. Consequently, 30-50% of the cells were lost upon inoculation into microcarrier suspension and significant fraction of the mirrocarrier became bald. The medium supplemented with the concentrated conditioned medium by hepatoma cell line HpG2 supported the active growth of the cells on microcarrier and the cells showed a very healthy and well spreading morphology. It was probable that some spreading and attachment factors of HpG2 conditioned medium were effective for 293 cells.

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Growth and Antioxidant Production of Bacillus polyfermenticus SCD in Whey Protein Concentrate (WPC)-based Medium (유청단백질농축물을 기본 배지로 한 Bacillus polyfermenticus SCD균의 생육과 항산화물질 생산)

  • Choi, Gooi-Hun;Lee, Jang-Hyun;Jo, Mi-Na;Yoon, Yo-Chang;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.28 no.1
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    • pp.105-108
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    • 2008
  • The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

The Effect of Spent Medium Recycle on Cell Proliferation, Metabolism and Baculovirus Production by the Lepidopteran Se301 Cell Line Infected at Very Low MOI

  • Beas-Catena, Alba;Sanchez-Miron, Asterio;Garcia-Camacho, Francisco;Contreras-Gomez, Antonio;Molina-Grima, Emilio
    • Journal of Microbiology and Biotechnology
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    • v.23 no.12
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    • pp.1747-1756
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    • 2013
  • The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch suspension cultures in Ex-Cell 420 serum-free medium. The results showed that the recycle of 20% of spent medium from a culture in mid-exponential growth phase improved growth relative to a control culture grown in fresh medium. Although both glucose and glutamine were still present at the end of the growth phase, glutamate was always completely exhausted. The pattern of the specific glucose and lactate consumption and production rates, as well as the specific glutamine and glutamate consumption rates, suggests a metabolic shift at spent medium recycle values of over 60%, with a decrease in the efficiency of glucose utilization and an increase in glutamate consumption to fuel energy metabolism. Baculovirus infection provoked a change in the metabolic pattern of Se301 cells, although a beneficial effect of spent medium recycle was also observed. Both growth rate and maximum viable cell density decreased relative to uninfected cultures. The efficiency of glucose utilization was dramatically reduced in those cultures containing the lowest percentages of spent medium, whereas glutamine and glutamate consumption was modulated, thereby suggesting that infected cells were devoted to virus replication, retaining their ability to incorporate the nutrients required to support viral replication. Recycle of 20% of spent medium increased baculovirus production by around 90%, thus showing the link between cell growth and baculovirus production.

Effects of the special media on the mycelium growth in Agaricus campestris (몇가지 식물이 Agaricus campestris 균사의 생장에 미치는 영향(예보))

  • 이덕봉
    • Journal of Plant Biology
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    • v.12 no.2
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    • pp.1-6
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    • 1969
  • Effects of the special media on the mycelium growth in Agaricus campestris were studied. The results might be summarized as follows: 1. The mycelium growth fo Agaricus campestris were scarecely stimulated on the Peptone basal medium which was added 0.5gr. of Peptone and Dextrose basal medium which was added 1.5gr. of dextrose during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts showed a considerable growth for 144 hours. The order is as follows; Carrot-basal medium(4ml./100ml.)>Tomato-basal medium(2ml./100ml.)>Spinach-basal medium(3ml./100ml.). However, the spinach-basal medium among these three media were no significant difference.

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A Study on the Mutual Effect between Small & Medium-sized Enterprises and Economic Growth: Evidence from Alibaba Group and City of Hangzhou

  • He, Yugang
    • Asian Journal of Business Environment
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    • v.9 no.2
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    • pp.27-34
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    • 2019
  • Purpose - From the advanced path of development and current situation, the development of enterprises plays a tremendous role in promoting national economic growth and raising the overall national strength. Therefore, this paper aims at examining the mutual effect between small & medium enterprises and economic growth. Research design, data, and methodology - In order to address the operating mutual effect between the small & medium enterprises and economic growth more clearly, this paper sets Alibaba Group and Hangzhou as an example. Meanwhile, the annual data from 2000 to 2017 will be employed, and an empirical analysis will be performed under the vector error correction model. Results - The findings display that the total revenue of Alibaba Group has a positive effect on economic growth in city of Hangzhou. However, the Granger Causality test implies that there is only a unidirectional causality between total revenue of Alibaba Group and economic growth in Hangzhou. More specifically, 1% increase in total revenue of Alibaba Group can result in 0.272% in economic growth of Hangzhou in the long run. Conclusions - In summary, for the long run, the local governments should promulgate a series of policies to assist the small & medium enterprises like Alibaba Group to improve the local economic growth as seen in the city of Hangzhou.

Optimization of Major Culture Elements on Growth and Shikonin Production in the Lithospermum erythrorhizon Hairy Root Culture

  • Hwang, Ok-Jin;Kim, Yu-Jeong;Sung, Nak-Sul;Ahn, Jun-Cheul;Kim, Sik-Eung;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.4
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    • pp.243-248
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    • 2002
  • The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.

Utilization of Domestic Vegetables Juices as a Medium for Growth and Reproduction of Phytophthora species (국내산 야채쥬스의 역병균 영양생장 및 생식생장용 배양기 이용)

  • 지형진;조원대;최용철
    • Korean Journal Plant Pathology
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    • v.14 no.4
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    • pp.299-302
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    • 1998
  • V8 juice agar (V8A) has been the most popular and commonly used medium for growth and reproduction of Phytophthora. However, frequently V8 vegetable juice is not available or difficult to obtain in Korea. We therefore developed widely accessible medium to substitute for V8A using domestically available five juices; two carrot (KCJA), two tomato (KTJA) and a vegetable-mix (KVMA). To prepare 10% juice medium, each vegetable juice 100 ml, DW 900 ml, agar 17 g and Ca CO3 0.5∼1.0g were supplemented to adjust pH ca. 6.0 Mycelial growth of P. cactorum and P. capsici on KTJA and KVMA was equally effective as V8A for the growth of P. cactorum, P. capsici, P. drechsleri and P. nicotianae under light. Sporangial production of P cactorum, P. capsici and P. nicotianae on KTJA and KVMA was as good as V8A and slightly better than CKJA, but the difference was insignificant by P. cactorum and P. nicotianae. The four fungi successfully formed oospores on all the media although the numbers were varied among species and media. While KTJA was the best for P. cactorum and P. capsici, V8A was the best for P. capsici and P. drechsleri. However, KCJA stimulated highest number of oopspores of P. nicotianae. Overall results showed that domestically available vegetable juices were highly effective on growth and reproduction of Phytophthora and comparable to V8 juice. Therefore, the domestic juice medium can be successfully replaced V8A in Phytophthora study.

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