• Research in Plant Disease
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• v.13 no.2
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• pp.104-109
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• 2007

Pink root disease of onion, a known worldwide constraint upon onion production, significantly reduces crop levels in the main Korea cultivation area. In order to examine the effect of flooding on incidence of pink root disease caused by Pyrenochaeta terrestris and on onion growth and on populations of soil fungi, field experiments were conducted during two seasons, 2003/04 and 2004/05 at Onion Research Institute. Populations of soil fungi from fields were assayed on selective media. Flooding treatment was effective in reducing populations of P. terrestris, Pythium spp., and Rhizoctonia sp. in soil; fungal populations in soils flooded for 90 days were reduced to 1/2 to 1/3 of those in non-flooded soils. In nursery bed, protective activities of soils flooded for over 60 days were 93.5 to 99.2% and their pink root incidences were less than 5%, which were 1/11 to 1/18 of that in control. Increased yield of onion bulbs was associated with control of soil borne pathogenic fungi by flooding treatments. As flooding period was prolonged, bulb grade showed the tendency to increase. Soil flooding for over 60 days resulted in effective disease control, facilitated accurate planning of plant population in the field, extension of the growing-season and consequently higher yields of better quality.

• Research in Plant Disease
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• v.13 no.2
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• pp.110-114
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• 2007

Anthracnose fungus was most pathogenic on soybean sprout, of the fungi and bacteria isolated from rotten sprout on market. Bacterial strains associated were not virulent. Dry heat (DHT) applied even as high as $65^{\circ}C$ for 30min. was not effective enough to eliminate the artificially inoculated Colletotrichum gloeosporioides propagules from seedllots. Hot water immersion treatment (HWT), at elevated temperature of $55^{\circ}C$ for 20 min, did eliminate the pathogen but reduced seed germinating and retarded sprout growth: Seed germination was practically acceptable when the seedlots were exposed to at $55^{\circ}C$ for 5 min, but about 20% anthracnose propagules survived. Accordingly, we have optimized the HWT scheme for 5 min at $60^{\circ}C$. This scheme was validated, at small to large scale production system, that surely rule out the possible carry over of the bacterial contaminant from seedlots. This result should improve the shelf-life of soybean sprout on the market.

• Research in Plant Disease
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• v.13 no.2
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• pp.122-125
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• 2007

Leaf spot disease of Kalanchoe (Kalanchoe blossfeldiana) occurred at the farmer's vinly house in Gangseo-gu, Busan Metropolitan City, Korea, 2006. The diseased plants with typical symptom were collected and the casual agent were isolated. Its mycological characteristics and pathogenicity were examined. The results were as follows. The typical symptoms of the disease appeared as small brownish or dark brown spot on both sides of the leaves. The spots tended to develop from lower leaves. The spots gradually enlarged into conspicuous necrotic lesions 1-5 mm in diameter. Colonies of the causal fungus formed on potato dextrose agar were velvety, gray or grayish brown in color, Conidia were cylindrical or obclavate to oblong in shape, brown in color, $24{\sim}65\;{\times}\;12{\sim}23\;{\mu}m$ in size and had 1-4 transverse septa, The optimum temperature for growth of the fungus was about $25-30^{\circ}C$. Conidiophores were brown in color, $32{\sim}135\;{\times}\;4{\sim}8\;{\mu}m$ in size and had 1-7 transverse septa. The fungus was identified as Stemphylium lycopersici (Enjoji) Yamamoto based on its symptom and mycological characteristics. This is the first report of leaf spot of Kalanchoe caused by S. lycopersici in Korea.

• Research in Plant Disease
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• v.21 no.3
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• pp.208-214
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• 2015

To develop an effective biopesticide to control pepper anthracnose disease, an isolate which showed strong inhibitory effect on the mycelial growth and conidial germination of Colletotrichum acutatum was selected among the antagonistic bacterial isolates collected from pepper grown soil. The bacterial isolate was identified as Bacillus sp. KBC1004 using 16S rRNA sequence analysis. The liquid culture of KBC1004 was freeze-dried and formulated as a wettable powder(WP). The wettable powder form of KBC1004 required at least 24 hours to activate and to inhibit the conidial germination of C. acutatum. In vitro bioassay using the detached green pepper fruits, biocontrol activity of the WP was not recognizable in simultaneous inoculation, but significant disease suppression was observed pre-treatment (24 hr) of the WP before pathogen inoculation. In field experiment, 4 times foliar applications of the 1/500 diluted wettable powder from the end of June showed great control efficacy similar to that of the chemical fungicide application. These results suggest that the formulated WP product could be an alternative mean to control of pepper anthracnose disease in environmentally friendly farming practices.

• Archives of Plastic Surgery
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• v.38 no.2
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• pp.135-142
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• 2011

Purpose: Deficiencies of the abdominal wall can be the a result of infection, surgery, trauma, or primary herniation. For abdominal wall reconstruction, synthetic materials have been shown to provide a better long-term success rate than primary fascial repair. But, synthetic materials cannot elicit angiogenesis or produce growth factor and are therefore plagued by an inability to clear infection. As a result of the inherent drawbacks of synthetic, significant effort has been spent on the identification of new bioprosthetic materials. The aim of our study is to evaluate the effectiveness of a synthetic material (PROCEED$^{(R)}$) and an ADM ($SureDerm^{TM}$) to repair abdominal wall defects in a rabbit models. Methods: We measured the tensile strength of the $SureDerm^{TM}$ and PROCEED$^{(R)}$ by a Tension meter (Instron 4482). 16 Rabbit models were assigned to this study for abdominal wall reconstruction. Abdominal defect of 8 rabbits were reconstructed by PROCEED$^{(R)}$ and the rest were reconstructed by $SureDerm^{TM}$. We assessed gross and histologic examinations for the reconstructed abdominal wall. Results: The tensile strength of $SureDerm^{TM}$ and Gore Tex$^{(R)}$ is $14.64{\pm}0.51Mpa$, $8.54{\pm}0.45Mpa$. PROCEED$^{(R)}$ was estimated above the limits of measurement. Inflammatory reaction of PROCEED$^{(R)}$ persisted for 32weeks, but $SureDerm^{TM}$ decreased after 16weeks. Vascular ingrowth into the $SureDerm^{TM}$ was seen after 32 weeks. The basement membrane of $SureDerm^{TM}$ changed into a form of pseudoperitoneum. In PROCEED$^{(R)}$, it seemed like pseudoepithelial lining was made from the fibrosis around the mesh. Conclusion: In our study, the $SureDerm^{TM}$ not only have less inflammatory reaction and presented more angiogenesis than the PROCEED$^{(R)}$, but also have pseudoperitoneum formation. It is expected that $SureDerm^{TM}$ is useful for abdominal wall reconstruction. However, a long-term study of its usage consequences are thought to be needed.

• Archives of Plastic Surgery
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• v.39 no.1
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• pp.46-50
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• 2012

Background : The purpose of our study was to analyze scientific papers published by South Korean plastic surgeons in journals included in the Science Citation Index Expanded (SCIE), and to evaluate the publication and research activities of Korean plastic surgeon. Methods : We conducted a survey of SCIE papers in the field of plastic surgery published by South Korean authors between 2001 and 2010 using Web of Science software. We further analyzed these results according to the number of publications per year, journals, institution, and type of papers. We also compared the total number of citations to published scientific papers. We analyzed the rank of South Korea among other countries in representative journals. Results : Overall, 667 papers were published by South Korean authors between 2001 and 2010. The number of publications increased dramatically from 2003 (n=31) to 2010 (n=139). Subsequently, the ten most productive Korean medical colleges were identified. All published papers received 2,311 citations and the citation to paper ratio was 3.49. The rank of Korea among other countries in terms of the number of published papers remained in the top 10 during the recent 10 years. Conclusions : Publication output of Korean plastic surgeon over the last 10 years showed a remarkable growth in terms of quantity and quality. Currently, Korea is among the top six countries in representative plastic surgery journals. Korean plastic surgeons have played a central role in this progress, and it is anticipated that they will continue to do so in the future.

• Archives of Plastic Surgery
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• v.40 no.5
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• pp.496-504
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• 2013

Background Amniotic-fluid-derived stem cells and amniocytes have recently been determined to have wound healing effects, but their mechanism is not yet clearly understood. In this study, the effects of amniotic fluid stem cells and amniocytes on wound healing were investigated through animal experiments. Methods On the back of Sprague-Dawley rats, four circular full-thickness skin wounds 2 cm in diameter were created. The wounds were classified into the following four types: a control group using Tegaderm disc wound dressings and experimental groups using collagen discs, amniotic fluid stem cell discs, and amniocyte discs. The wounds were assessed through macroscopic histological examination and immunohistochemistry over a period of time. Results The amniotic fluid stem cell and amniocyte groups showed higher wound healing rates compared with the control group; histologically, the inflammatory cell invasion disappeared more quickly in these groups, and there was more significant angiogenesis. In particular, these groups had significant promotion of epithelial cell reproduction, collagen fiber formation, and angiogenesis during the initial 10 days of the wound healing process. The potency of transforming growth factor-${\beta}$ and fibronectin in the experimental group was much greater than that in the control group in the early stage of the wound healing process. In later stages, however, no significant difference was observed. Conclusions The amniotic fluid stem cells and amniocytes were confirmed to have accelerated the inflammatory stage to contribute to an enhanced cure rate and shortened wound healing period. Therefore, they hold promise as wound treatment agents.

• Korean Journal of Poultry Science
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• v.12 no.2
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• pp.59-64
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• 1985

The study was carried out to determine if a difference in initial chick weight was still evident in market body weight(6 or 8 week body weight) of broilers. The data of a total of 800 broiler commercial chick raised at Poultry Breeding Farm, Seoul National University from Aug. 6, 1981 to Oct. 1, 1981 were analysed. The results were summarized as follows: 1. Although the initial chick weight was about the same, male chick grew more rapidly than female and sex difference was the most striking factor in growth rate of broiler in this analysis. 2. The relationship between initial chick weight and body weight after hatching was decreased to the increase of weeks of age in both sexes. Though the correlation coefficients of the 6 week and 8 week body weight to the initial chick weight were 0.15 & 0.15 in female and 0.18 & 0.17 in male, the relationship was highly significant in both sexes. 3. Regressions of 6 and 8 week body weight on the initial chick weight in both sexes indicate that, for each increase of one gram in weight of chick at hatching, the average increase in body weight was 8.33 gram ＆ 10.07 gram in female and 7.85 gram ＆ 11.28 gram in male. Results show that the initial chick weight (1 day of age) influence average body weight of commercial broiler at 6 and 8 weeks of age.

• Korean Journal of Poultry Science
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• v.30 no.4
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• pp.253-257
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• 2003

The suitability of processed cardboard(PC) as litter of broiler house was evaluated by comparing the growth performances of broiler chicks reared on the PC with those of birds reared on conventional rice hull litter(RH) as the control group. A total of 180 male broiler chick (5d old, Ross) were randomly divided into 2 groups and placed into 2 types of litter, PC and RH, with 3 replicates of 30 birds per replicate. Commercial broiler feeds and water were provided ad libitum for 5 weeks. The body weight gains of the birds reared on PC were heavier than those on RH(P<0.05). The average feed conversion rate of chicks reared on PC was appeared to be improved as compared to that of the control. The Enterobacter aerogens counts in PC were significantly rduced than those in the control litter(P<0.05), but a total number of Salmonella and E. coli were not affected by the litter materials. No difference was observed between the average moisture contents of the litters. The average mortality of the chicks reared on PC was significantly lower than that of RH(P<0.05). The results indicate that processed cardboard can substitute other conventional litter materials when the others are in short supply or expensive.

• The Journal of Korean Physical Therapy
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• v.20 no.1
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• pp.57-65
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• 2008

Purpose: Heat shock proteins (HSPs) are a group of proteins that are activated when cells are exposed to a variety of environmental stresses, such as infection, inflammation, exposure to toxins, starvation, hypoxia, brain injury, or water deprivation. The activation of HSPs by environmental stress plays a key role in signal transduction, including cytoprotection, molecular chaperone, anti-apoptotic effect, and anti-aging effects. However, the precise mechanism for the action of small HSPs, such as HSP27 and mitogen-activated protein kinases (MAPKs: extracellular-regulated protein kinase 1/2 (ERK1/2), p38MAPK, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), is not completely understood, particularly in application of cell stimulators including platelet-derived growth factor (PDGF), angiotensin II (AngII), tumor necrosis factor $\alpha$ (TNF$\alpha$), and $H_2O_2$. This study examined the relationship between stimulators-induced enzymatic activity of HSP27 and MAPKs from rat smooth and skeletal muscles. Methods: 2-dimensional electrophoresis (2DE) and matrix assisted laser desorption ionizationtime-of-flight/time-of-flight (MALDI-TOF/TOF) analysis were used to identify HSP27 from the intact vascular smooth and skeletal muscles. Three isoforms of HSP27 were detected on silver-stained gels of the whole protein extracts from the rat aortic smooth and skeletal muscle strips. Results: The expression of PDGF, AngII, TNF$\alpha$, and $H_2O_2$-induced activation of HSP27, p38MAPK, ERK1/2, and SAPK/JNK was higher in the smooth muscle cells than the control. SB203580 (30${\mu}$M), a p38MAPK inhibitor, increased the level of HSP27 phosphorylation induced by stimulators in smooth muscle cells. Furthermore, the age-related and starvation-induced activation of HSP27 was higher in skeletal muscle cells (L6 myoblast cell lines) and muscle strips than the control. Conclusion: These results suggest, in part, that the activity of HSP27 and MAPKs affect stressors, such as PDGF, AngII, TNF$\alpha$, $H_2O_2$, and starvation in rat smooth and skeletal muscles. However, more systemic research will be needed into physical therapy, including thermotherapy, electrotherapy, radiotherapy and others.