• 제목/요약/키워드: Growth, Reproduction

검색결과 696건 처리시간 0.026초

Growth Rates of Transgenic Mice Containing Growth Hormone Receptor Gene

  • Kim, H. J;K. Naruse;S. M. Chang;K. S. Im;Lee, S. H.;Park, C. S.;D. I. Jin
    • 한국가축번식학회지
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    • 제27권4호
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    • pp.333-338
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    • 2003
  • Transgenic mice containing GH Receptor (GHR) gene fused to metallothionein promoter were analyzed to evaluate effect of GHR expression on growth in vivo. Three founder mice lines contained copies of GHR transgene and transmitted these genes into F$_1$ and F$_2$ progenies. The mRNA expression of transgene was identified using RT-PCR with GHR genes in tissues. To analyze the effects of transgenes on growth performance, body weights of pups were measured at 4, 10 and 14 weeks after birth. The body weight of transgenic mice was higher compared with that of non-transgenic control mice regardless of sex (P<0.05). Body weights between transgenic and non-transgenic mice were increased with aging. Overall, GHR transgenic mice tended to grow about 10 to 15 % faster than non-transgenic mice without any pathological defects.

Mapping of Quantitative Trait Loci Affecting Growth Traits in a Japanese Native Chicken Cross

  • Rikimaru, K.;Sasaki, O.;Koizumi, N.;Komatsu, M.;Suzuki, K.;Takahashi, Hideaki
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권10호
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    • pp.1329-1334
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    • 2011
  • The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

소 체외수정란의 발생배양에 적합한 배양환경 조성 연구 I. 체세포, 성장인자 또는 배양액 종류가 난포란의 체외성숙에 미치는 효과 (A Study on Culture Environments of In Vitro Matured/In Vitro Fertilized Bovine Embryos I. Influence of Somatic Cells, Growth Factors or Culture Media on In Vitro Maturation of Bovine Oocytes)

  • 이명식;박수봉;박진기;장원경;민관식;백광수;성환후;박용윤
    • 한국가축번식학회지
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    • 제22권1호
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    • pp.95-99
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    • 1998
  • Three experiments were conducted with follicular oocytes, to compare some somatic cells, growth factors and media for in vitro maturation of bovine oocytes. In the first experiment, the type of somatic cells had no effects on in vitro maturation of bovine follicular ooctyes. In the second experiment, oocytes were matured in TCM199 su, pp.emented with growth factors on IVM of bovine follicular oocytes, then all were co-cultured with cumulus cells. The proportion of used oocytes that developed to expanding blastocysts was 22.2%, 20.2%, 17.7%, 22.2%, 24.4% and 20.2% after maturation in TCM199 su, pp.emented with control, insulin, IGF-I, IGF-Ⅱ, FGF and EGF, respectively. In the third experiment, oocytes were matured in BO, Ham's F10 and TCM199, then all were fertilized in BO, and embryos cultured in BO, Ham's F10 and TCM199, respectively. Cleavage rates in BO were 90%, had higher than in Ham's F10(80%) or in TCM199(64%). But production of expanding blastocysts in TCM199(21%) or Ham's F10(20.6%), had higher than in BO(4.6%).

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형질전환동물의 유선조직으로부터 인간 성장호르몬의 분비 (Secretion of Human Growth Hormone from Mammary Gland of Transgenic Mice)

  • 구덕본;최강덕;정형민;이상민;이경광;이훈택;정길생
    • 한국가축번식학회지
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    • 제17권4호
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    • pp.375-383
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    • 1994
  • The human growth hormone (hGH) gene uder the control of the rat $\beta$-casein promoter gene was designed to produce transgenic mouse expressed hGH gene in only mammary gland. One hundred seventy two eggs microinjected were transferred to the oviducts of pseudopregnants and 43 offspring were delivered. By Southern blotting hybridization, 3 were transgenic with rat $\beta$-casein/hGH gene. The copy numbers of three transgenic founder were 1, 5, and 15, respectively. A radioimmunoassay was developed to quantitate the amount of expression of the hGH gene in mammary gland of transgenic mice. The amount of hGH was 13.3ng/ml in the lactating milk of one transgenic line, showing predominantly higher than 3.0ng/ml in milk of control mice. Therefore, our findings suggested that $\beta$-casein promoter may induce the tissue specific expression of structural gene.

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생쥐, 쥐 및 돼지의 난소내 난포의 폐쇄에 관한 조직화학적 연구 (Histochemical Study of the Atresia of Ovarian Follicles)

  • 김종흡;김성인;윤용달;김문규
    • 한국가축번식학회지
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    • 제16권2호
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    • pp.165-173
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    • 1992
  • In order to study the mechanism of follicle growth and maturation, and also to supplement the criteria identifying the follicle state of normal of atretic, the histochemical investigation on the ovarian follicles according to the ovarian cycle of mouse, rat and pig has been done. The intercellular space of granulosa cells, especailly Call-Exner body, and follicular fluid in the antrum showed positive to PAS, and blue stain by trichrome dye. The resutls suggest that the mucous polysaccharide was synthesized by the granulosa cells, and secreted into the antrum through Call-Exner body so as to be the components of the follicular fluid as the follicles proceeded to growth and maturation. The further the follicles proceeded to atresia the more densely their theca externa were stained blue by follicles proceeded to atresia the more densely their theca externa were stained blue by trichrome dye, and the more densely the granulosa cells were stained red by oil red 0 dye. Therefore, these staining methods can be applied to the criteria identifying the follicle atresia.

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Unusual Rhizoidal Development in Bangia (Bangiales, Rhodophyta) -Another Form of Vegetative Reproduction?

  • Boedeker, Christian;Farr, Tracy J.;Nelson, Wendy A.
    • ALGAE
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    • 제22권1호
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    • pp.31-36
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    • 2007
  • The gametophytic filaments of two genetically distinct taxa of Bangia from New Zealand showed unusual rhizoidal development in comparative culture experiments. In the past Bangia has been reported to possess simple, colourless rhizoids that extend from the basal cells of the unbranched filaments, whereas in this study the rhizoids observed became pigmented and multicellular. A reversal of growth direction occurred and filamentous extensions developed from the rhizoids under some culture conditions. These extensions were either prostrate or resembled new gametophytic filaments. This is the first report for filamentous members of the Bangiales of the development of such stolon-like rhizoids, apparently serving as a form of vegetative reproduction.

A Special Case of a Two-Sex Model in the Growth of Population

  • Tae Ryung Park
    • Communications for Statistical Applications and Methods
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    • 제4권1호
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    • pp.207-218
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    • 1997
  • We consider two models for the growth of population with overlaping generations. First, the model we will describe is basically the model given by Leslie(1945). This is only a one-sex model of population age structure and growth. Next, we introduce a model in which couples must be formed before reproduction occurs. If the maximum number of couples is formed, and if the couples are only formed from fermales of age x-a and males of age x at time t, $\alpha$ > 0. Then, we will solve the renewal equations for the reproductive value.

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The Influence of Weaning Time on Deer Performance

  • Bao, Y.M.;Ru, Y.J.;Glatz, P.C.;Miao, Z.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권4호
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    • pp.569-582
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    • 2004
  • Current growth in the deer industry is 20% per annum as the demand increases for venison in Europe, including the increased use in Asia of antlers as a natural medicine by humans. The deer industry in Australia has developed rapidly, but farm profitability has fluctuated markedly. The knowledge on deer farming has largely been adopted from New Zealand (NZ) and the United Kingdom (UK) although the environmental conditions in these deer growing countries differ markedly from Australia. The practice used for weaning is one of the key factors influencing the profitability of the industry. However, a wide range in weaning dates are practised by farmers leading to the question of whether deer should be weaned after mating to reduce the stress during weaning or should they be weaned in the pre-rut period to allow does to recover their body condition for the next reproduction cycle. This review examines the effect of weaning time on the performance of does and hinds and the subsequent growth rate of the fawns and calves. Gaps in knowledge of weaning procedures and nutritional management for early weaned deer are identified.