• Title/Summary/Keyword: Group separation

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Quantitative Analysis of Marker Substances of Paeonia lactiflora by Solid Fermentation (작약의 고체발효에 따른 지표성분의 함량분석)

  • Lee, Ji-Hye;Um, Young-Ran;Park, Hwa-Yong;Lee, Jae-Hoon;Ma, Jin-Yeul
    • Korean Journal of Oriental Medicine
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    • v.15 no.2
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    • pp.119-124
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    • 2009
  • The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230nm). The flow rate was 1.0 ml/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was $0.090\;{\pm}\;0.03%$ in P. lactiflora extract(control), $0.102\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Paecilomyces japonica, $0.056\;{\pm}\;0.01%$ in P. lactiflora extract fermented with Ganoderma lucidum, $0.093\;{\pm}\;0.00%$ in P. lactiflora extract fermented with honey and $0.046\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was $4.506\;{\pm}\;0.13%$ in control, $2.599\;{\pm}\;0.04%$ in P. lactiflora extract fermented with Paecilomyces japonica, $1.222\;{\pm}\;0.03%$ in P. lactiflora extract fermented with Ganoderma lucidum, $2.750\;{\pm}\;0.05%$ in P. lactiflora extract fermented with honey and $0.847\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.

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A Proposal of Efficient Method for Data Center Information System Migration (데이터센터 정보시스템 이전을 위한 효율적인 방안 제시)

  • Song, Min-Gu;Kim, Sun-Bae
    • Journal of Digital Convergence
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    • v.12 no.3
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    • pp.201-210
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    • 2014
  • Recently, the more complicated with the elements made of information system, the more dangerous in migration them. So it is need to accomplish information system migration systematically. According to the movement of public institution, a large scale of information system migration is in processing and will be made. In this paper, I would like to suggest the use of migration solution in order to speed & effect information system migration process. This will be provided the pursuit function of information system movement, movement location pursuit in real time, monitoring function through the situation room, separation migration possibility on the basis of business attribute. Therefore, if you use the suggested solution, it will be decreased trial and error & minimized the movement time.

A phylogenetic analysis of the genus Pilea (Urticaceae) using nrDNA and cpDNA sequences (한국산 물통이속(Pilea) 식물의 nrDNA, cpDNA를 통한 계통분석)

  • Moon, Ae-Ra;Park, Jeong-Mi;Jang, Chang-Gee
    • Korean Journal of Plant Taxonomy
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    • v.45 no.2
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    • pp.158-168
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    • 2015
  • A study of the genus Pilea in Korea including five taxa was carried out using molecular phylogenetic methods. The majority of members of the genus Pilea in Korea are annual herbs, and they live in moist habitats, flowering in summer and fruiting in autumn. The results of a phylogenetic analysis using nrDNA and cpDNA supported the recognition of P. japonica, P. peploides, and P. taquetii. Pilea taquetii from Mt. Sanbangsan in Jeju was nested within P. hamaoi and P. mongolica clade instead of the P. taquetii clade, with P. taquetii from Mt. Jirisan also separated from the P. taquetii clade. This indicates that the separation is not geographical isolation, but is instead related to taxonomic problems. Therefore, further study of the P. taquetii group is necessary.

Authors Under the Service of the Army in the Korean War (한국전쟁기 육군종군작가단의 작품 활동)

  • 신영덕
    • Lingua Humanitatis
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    • v.1 no.2
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    • pp.197-217
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    • 2001
  • The literary products of Authors Under the Service of the Army during the Korean War have been neglected on the whole because of the perception that they were little more than war propaganda. The majority of the works (poetry, serial novels, and short stories) Published by these authors in various Army publications such as Junsunmunhak (Literature of the War Front) and Comet, as well as in regular literary periodicals, supports this perception. Most of these works convey simplistic emotions and stereotypes that project untroubled patriotism and strongly antipathetic sentiments against to the Communist North. The appointed leader of this group, Dock-Kyun Choi regarded the pen as another form of weapon to be used against the Communists in the North, and did not shy away from describing in graphic details the atrocities committed by his enemies. But what truly deserves our attention is the fact that many of the same authors who wrote highly propagandistic works also wrote works that can only be described as antiwar. In these works are depicted as faithfully as possible the human sufferings of the war. These works resist and even question the very ideologies that have brought about the conflict, focusing instead on the dark side of the war -the horrifying deaths, the separation of families, and the displacement of people from their homes, How we are to interpret this ambivalence in many of these authors is a task that remains to be carried out. We must approach these works with more seriousness and begin by comparing them with similar products from authors under the service of the Navy and the Air Force during the Korean War.

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Crystal structure of Mn-Co-Ni thermistor (Mn-Co-Ni 서미스터의 결정구조 분석)

  • Lee, Jung-Il;Mhin, Sungwook;Ryu, Jeong Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.5
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    • pp.225-229
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    • 2015
  • This study was performed to investigate crystal structure of cubic spinel-type monophase oxide composed of the Mn-Co-Ni ternary system. Starting material was prepared by mixing Mn, Co, Ni oxides then evaporation to dryness. The XRD patterns were analyzed by in-situ XRD as increasing temperature from room temperature to $1400^{\circ}C$ in air atmosphere. The cubic spinel phase was existed in a temperature range from $900^{\circ}C$. However, separation of NiO phase was detected from $1300^{\circ}C$, which was the origin of deterioration in the crytallinity. The surface morphology of the manufactured NTC thermistors were analyzed by FE-SEM for comparison of good and bad samples.

The Crystal Structure of Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II) (Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II)의 결정구조)

  • Go, Gi-Yeong;Nam, Gung-Hae;Han, Sang-Gon
    • Korean Journal of Crystallography
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    • v.9 no.1
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    • pp.71-76
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    • 1998
  • Crystal structure of Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II0 has been determined by X-ray crystallography. Crystal data : (Pd(C2H8N2)2.Pd(C2O4)2), Fw=509.04, Monocline, Space Group P21/c (no=14), a=6.959(2), b=13.506(2), c=15.339(2) Å, β=99.94(3), Z=4, V=1420 Å3, Dc=2.380 gcm-3, μ=25.46cm-1, F(000)=992. The intensity data were collected with Mo-Kα radiation (λ=0.7107 Å) on an automatic four-circle diffractometer with a graphite monochromater. The structure was solved by Patterson method and refined by full matrix least-square methods using unit weights. The final R and S values were R=0.021, Rw=0.030, Rall=0.032 abd S=2.1 for 1472 observed reflections. The essentially planar complex anions form diade of interplanar distances of 3.41 Å and their diads are stacked along aaxis with interplanar separation of 3.44 Å.

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Relationships between Work-related Psychosocial and Acculturative Factors and Work-related Musculoskeletal Disorders among Korean-Chinese Migrant Workers Living in Korea (조선족 근로자의 직업 관련성 사회 심리적 요인, 문화적응 요인과 직업 관련성 근골격계 질환의 관계)

  • Kim, Sun-Jung;Lee, Hyeon-Kyeong;Ahn, Hyun-Mi
    • Korean Journal of Occupational Health Nursing
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    • v.19 no.1
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    • pp.28-40
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    • 2010
  • Purpose: The purpose of the study >was to examine the prevalence of work-related musculoskeletal disorders(WMSDs) and to identify the relationship between work-related psychosocial and acculturative factors and WMSDs among Korean-Chinese workers living in Korea. Methods: A cross-sectional survey was conducted with 195 Korean-Chinese workers who have worked full-time for the past 6 months. A structured questionnaire, including measures for musculoskeletal symptoms, physical demand, work-related psychosocial(job demand, job control, interpersonal conflict), acculturative (acculturation strategy and acculturative stress), and personal factors, was used. Descriptive statistics and logistic regression analysis were conducted using the SPSS WIN 17.0. Results: The prevalence of WMSDs was 64.1%. In comparison to Korean workers, job demand of this group was lower while both interpersonal conflict and lack of job control were higher among them. Integration was the most commonly used acculturation strategy and mean scores of acculturative stress were below the median. Acculturative stress was positively correlated with integration and assimilation while negatively correlated with separation and marginalization. Interpersonal conflict and integration appeared to be related to WMSDs among male and female Korean-Chinese workers, respectively. Conclusion: The WMSD interventions need to be planned in consideration of different influences of work-related psychosocial and acculturative factors on WMSDs by gender.

Synthesis and Characterization of Dinuclear Ni(II) Complexes with Tetraazadiphenol Macrocycle Bearing Cyclohexanes

  • Kim, Ki-Ju;Jung, Duk-Sang;Kim, Duk-Soo;Choi, Chi-Kyu;Park, Ki-Min;Byun, Jong-Chul
    • Bulletin of the Korean Chemical Society
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    • v.27 no.11
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    • pp.1747-1751
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    • 2006
  • The dinuclear tetraazadiphenol macrocyclic nickel(II) complexes [$Ni_2$([20]-DCHDC)]$Cl_2$ (I), [$Ni_2$([20]-DCHDC)]$(ClO_4)_2{\cdot}2CH_3CN $ (II(b)) and [$Ni_2$([20]-DCHDC)$(NCS)_2$] (III) {$H_2$[20]-DCHDC = 14,29-dimethyl-3,10,18,25-tetraazapentacyclo-[25,3,1,$0^{4,9}$,$1^{12,16}$,$0^{19,24}$]ditriacontane-2,10,12,14,16(32),17,27(31), 28,30-decane-31,32-diol} have been synthesized by self-assembly and characterized by elemental analyses, conductances, FT-IR and FAB-MS spectra, and single crystal X-ray diffraction. The crystal structure of II(b) is determined. It crystallizes in the monoclinic space group P2(1)/c. The coordination geometries around Ni(II) ions in I and II(b) are identical and square planes. In complex III each Ni(II) ion is coordinated to $N_2O_2$ plane from the macrocycle and N atoms of NCS- ions occupying the axial positions, forming a square pyramidal geometry. The nonbonded Ni…Ni intermetallic separation in the complex II(b) is 2.8078(10) $\AA$. The FAB mass spectra of I, II and III display major fragments at m/z 635.1, 699.4 and 662.4 corresponding to [$Ni_2$([20]-DCHDC)(Cl + 2H)]$^+$, [$Ni_2$([20]-DCHDC)$(ClO_4\;+\;2H)]^+$ and [$Ni_2$([20]-DCHDC)(NCS) + 6H]$^+$, respectively.

Changes in gene expression associated with oocyte meiosis after $Obox4$ RNAi

  • Lee, Hyun-Seo;Kim, Eun-Young;Lee, Kyung-Ah
    • Clinical and Experimental Reproductive Medicine
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    • v.38 no.2
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    • pp.68-74
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    • 2011
  • Objective: Previously, we found that oocyte specific homeobox (Obox) 4 plays significant role in completion of meiosis specifically at meiosis I-meiosis II (MI-MII) transition. The purpose of this study was to determine the mechanism of action of $Obox4$ in oocyte maturation by evaluating downstream signal networking. Methods: The $Obox4$ dsRNA was prepared by $in$ $vitro$ transcription and microinjected into the cytoplasm of germinal vesicle oocytes followed by $in$ $vitro$ maturation in the presence or absence of 0.2 mM 3-isobutyl-1-metyl-xanthine. Total RNA was extracted from 200 oocytes of each group using a PicoPure RNA isolation kit then amplified two-rounds. The probe hybridization and data analysis were used by Affymetrix Gene-Chip$^{(R)}$ Mouse Genome 430 2.0 array and GenPlex 3.0 (ISTECH, Korea) software, respectively. Results: Total 424 genes were up (n=80) and down (n=344) regulated after $Obox4$ RNA interference (RNAi). Genes mainly related to metabolic pathways and mitogen-activated protein kinase (MAPK) signaling pathway was changed. Among the protein kinase C (PKC) isoforms, PKC-alpha, beta, gamma were down-regulated and especially the MAPK signaling pathway PKC-gamma was dramatically decreased by $Obox4$ RNAi. In the cell cycle pathway, we evaluated the expression of genes involved in regulation of chromosome separation, and found that these genes were down-regulated. It may cause the aberrant chromosome segregation during MI-MII transition. Conclusion: From the results of this study, it is concluded that $Obox4$ is important upstream regulator of the PKC and anaphase-promoting complex action for maintaining intact germinal vesicle.

Evacuation Analysis for a Passenger Ship Using a Cellular Automata Model with Group Behavior (집단 행동을 나타내는 Cellular Automata 모델을 사용한 여객선 승객 탈출 분석)

  • Cha, Ju-Hwan;Ha, Sol;Lee, Kyu-Yeul
    • Journal of the Korea Society for Simulation
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    • v.20 no.4
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    • pp.149-155
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    • 2011
  • This paper presents simulations of advance evacuation analysis using a cellular automata model for passenger behavior in an emergency of passenger ship. The proposed cellular automata model divides the space in a uniform grid called "cell." Each passenger is located in a cell and moves to another cell according to a set of local rules assumed to be associated with the individual and crowd behaviors of the passengers. To verify the usefulness of the proposed cellular automata model, 11 tests, all of which are specified in International Maritime Organization Maritime Safety Committee/ Circulation 1238 (IMO MSC/Circ. 1238), were implemented, and it was confirmed that all the requirements of these tests had been met.