• The Korean Journal of Mycology
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• v.41 no.2
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• pp.85-90
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• 2013

This study investigated the internal and external morphological characteristics of decomposition and browning of oak sawdust medium for ground bed cultivation of Lentinula edodes. Within fifty days after L. edodes inoculation, surface hyphae on the bed browned. In 110 days, the fungal hyphae occupied and decomposed wood fibers, vessels and parenchymatous cells from the inside as white profuse hyphal mass was amorphously dissolving the saw dust particles from the outer surface. Most of the white hyphal bed surface became cleanly brown, however, some colony surface became blackened and slimy with contaminating bacteria, hyphae and spores. The brown layer was ca. 0.34 mm thick with highly dense and white hyphal mass beneath, whereas the blackened layer was ca. 1.17 mm thick with shrunken hyphae and less decomposed sawdust particles beneath. The surface hardness of the brown surface was ca. $0.73kgf/cm^2$, soft and resilient, while that of the blackened was ca. $0.91kgf/cm^2$, hard and nonresilient. By 150 days Lentinula edodes mushrooms fruited only on the brown surface and not on the blackened medium.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.221-230
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• 2015

Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.