Browse > Article
http://dx.doi.org/10.7845/kjm.2015.5039

Microbial community structures in the ground bed cultivation of Lentinula edodes using oak sawdust  

Shin, Ji-Hye (Department of Microbiology, Chungbuk National University)
Yun, Seo-Yeon (Department of Microbiology, Chungbuk National University)
Nam, Ji-Hyun (Department of Environmental Engineering and Energy, Myongji University)
Koo, Chang-Duck (Department of Forest Science, Chungbuk National University)
Lee, Dong-Hun (Department of Microbiology, Chungbuk National University)
Publication Information
Korean Journal of Microbiology / v.51, no.3, 2015 , pp. 221-230 More about this Journal
Abstract
Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.
Keywords
Amycolatopsis; Lentinula edodes; composting; oak sawdust; shiitake mushroom;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Allgaier, M., Reddy, A., Park, J.I., Ivanova, N., D'Haeseleer, P., Lowry, S., Sapra, R., Hazen, T.C., Simmons, B.A., Vander Gheynst, J.S., et al. 2010. Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community. PLoS One 5, 1-9.
2 Badham, E.R. 1991. Growth and competition between Lentinus edodes and Trichoderma harzianum on sawdust substrates. Mycologia 83, 455-463.   DOI
3 Bala, S., Khanna, R., Dadhwal, M., Prabagaran, S.R., Shivaji, S., Cullum, J., and Lal, R. 2004. Reclassification of Amycolatopsis mediterranei DSM 46095 as Amycolatopsis rifamycinica sp. nov. Int. J. Syst. Evol. Microbiol. 54, 1145-1149.   DOI
4 Chun, J., Huq, A., and Colwell, R.R. 1999. Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus. Appl. Environ. Microbiol. 65, 2202-2208.
5 Duchaine, C., Mériaux, A., Brochu, G., Bernard, K., and Cormier, Y. 1999. Saccharopolyspora rectivirgula from Quebec dairy barns: Application of simplified criteria for the identification of an agent responsible for farmer's lung disease. J. Med. Microbiol. 48, 173-180.   DOI
6 Fergus, C.L. 1964. Thermophilic and thermotolerant molds and actinomycetes of mushrom compost during peak heating. Mycol. Soc. Am. 56, 267-284.
7 Finstein, M.S., Miller, F.C., Strom, P.F., MacGregor, S.T., and Psarianos, K.M. 1983. Composting ecosystem management for waste treatment. Bio. Technol. 1, 347-353.   DOI
8 Fode-vaughan, K.A., Wimpee, C.F., Remsen, C., and Lynne, M. 2001. Detection of bacteria in environmental samples by direct PCR without DNA extraction research report. Biotechniques 31, 598-607.
9 Goodfellow, M., Kim, S.B., Minnikin, D.E., Whitehead, D., Zhou, Z.H., and Mattinson-Rose, A.D. 2001. Amycolatopsis sacchari sp. nov., a moderately thermophilic actinomycete isolated from vegetable matter. Int. J. Syst. Evol. Microbiol. 51, 187-193.   DOI
10 Hong, J.S. 1980. Nutrition value and medicine efficacy of mushroom. Food Ind. 53, 79-83.
11 Iiyama, K., Stone, B.A., and Macauley, B.J. 1994. Compositional changes in compost during composting and growth of Agaricus bisporus. Appl. Environ. Microbiol. 60, 1538-1546.
12 Jess, S., Murchie, A.K., and Bingham, J.F.W. 2007. Potential sources of sciarid and phorid infestations and implications for centralised phases I and II mushroom compost production. Crop. Prot. 26, 455-464.   DOI
13 Kim, J., Chun, J., and Han, H.U. 2000. Leuconostoc kimchii sp. nov., a new species from kimchi. Int. J. Syst. Evol. Microbiol. 50, 1915-1919.   DOI
14 Koo, C.D., Lee, H.Y., and Lee, G.Y. 2012. Effects of sterilization and cultivation temperatures of oak sawdust medium on Lentinula edodes hyphal growth. J. Korean For. Soc. 101, 77-82.
15 Koo, C.D., Lee, S.J., and Lee, H.Y. 2013. Morphological characteristics of decomposition and browning of oak sawdust medium for ground bed cultivation of Lentinula edodes. Korean J. Mycol. 41, 85-90.   DOI
16 Korea Forest Service. 2012. Statistical yearbook of forestry. 40, 1-494.
17 Lacey, J. 1973. Actinomycetales: characteristics and practical Importance. pp. 231-251. In Sykes, G. and Skinner, F. (eds.), Academic Press, New York, USA.
18 Lacey, J. 1997. Actinomycetes in composts. Ann. Agric. Environ. Med. 4, 113-121.
19 Lee, B., Bak, W., Ka, K., Yoon, K., and Ryu, S. 2007. Harmful microorganisms occurred on the bed-logs of several Quercus spp. for shiitake cultivation. Korean J. Mycol. 35, 33-36.   DOI
20 Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115-175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons, Chichester, England.
21 Miller, D.N., Bryant, J.E., Madsen, E.L., and Ghiorse, W.C. 1999. Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Appl. Environ. Microbiol. 65, 4715-4724.
22 Moore, D. and Chiu, S.W. 2001. Fungal products as food, pp. 1-17. In Pointing, S. and Hyde, K. (eds.), Bio-exploitation of filamentous fungi. Fungal Diversity press. Hong Kong, China.
23 Nakamura, L.K., Blumenstock, I., and Claus, D. 1988. Taxonomic study of Bacillus coagulans hammer 1915 with a proposal for Bacillus smithii sp. nov. Int. J. Syst. Bacteriol. 38, 63-73.   DOI
24 Park, K.S. and Lee, B.L. 1997. Extraction and separation of proteinbound polysaccharide by Lentinus edodes. Korean J. Food Nutr. 10, 503-508.
25 Park, W.C., Yoon, G.H., Kim, S.C., and Hong, G.S. 2008. New cultivation technology for sustainable production of Lentinula edodes. pp. 307. National Forest Research Institute. Seoul, Korea.
26 Partanen, P., Hultman, J., Paulin, L., Auvinen, P., and Romantschuk, M. 2010. Bacterial diversity at different stages of the composting process. BMC Microbiol. 10, 94.   DOI
27 Pranamuda, H., Tokiwa, Y., and Tanaka, H. 1997. Polylactide degradation by an Amycolatopsis sp. Appl. Environ. Microbiol. 63, 1637-1640.
28 Ryckeboer, J., Mergaert, J., Coosemans, J., Deprins, K., and Swings, J. 2003b. Microbiological aspects of biowaste during composting in a monitored compost bin. J. Appl. Microbiol. 94, 127-137.   DOI
29 Prażmo, Z., Dutkiewicz, J., and Cholewa, G. 2000. Gram-negative bacteria associated with timber as a potential respiratory hazard for woodworkers. Aerobiologia 16, 275-279.   DOI
30 Ryckeboer, J., Mergaert, J., Vaes, K., Klammer, S., Clercq, D., Coosemans, J., Insam, H., and Swings, J. 2003a. A survey of bacteria and fungi occurring during composting and self-heating processes. Ann. Microbiol. 53, 349-410.
31 Sanchez, C. 2004. Modern aspects of mushroom culture technology. Appl. Microbiol. Biotechnol. 64, 756-762.   DOI
32 Steger, K., Jarvis, Å., Vasara, T., Romantschuk, M., and Sundh, I. 2007. Effects of differing temperature management on development of Actinobacteria populations during composting. Res. Microbiol. 158, 617-624.   DOI
33 Straatsma, G., Gerrits, J.P.G., Augustijn, M.P.A.M., Op Den Camp, H.J.M., Vogels, G.D., and Van Griensven, L.J.L.D. 1989. Population dynamics of Scytalidium thermophilum in Mushroom compost and stimulatory effects on growth rate and yield of Agaricus bisporus. Microbiology 135, 751-759.   DOI
34 Straatsma, G., Olijnsma, T.W., Gerrits, J.P.G., Griensven, L.J.L.D., Samson, R.A., and Op Den Camp, H.J.M. 1995. Bioconversion of cereal straw into mushroom compost. Can. J. Bot. 73, 1019-1024.
35 Tamura, T., Ishida, Y., Otoguro, M., and Suzuki, K.I. 2010. Amycolatopsis helveola sp. nov. and Amycolatopsis pigmentata sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 60, 2629-2633.   DOI
36 Tokimoto, K. and Komatsu, M. 1979. Effect of carbon and nitrogen sources in media on the hyphal interference between Lentinus edodes and some species of Trichoderma. Japanese J. Phytopathol. 45, 261-264.   DOI
37 Tamura, K., Stecher, G., Paterson, D., Filipski, A., and Kumar, S. 2013. Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729.   DOI   ScienceOn
38 Tang, J.C., Shibata, A., Zhou, Q., and Katayama, A. 2007. Effect of temperature on reaction rate and microbial community in composting of cattle manure with rice straw. J. Biosci. Bioeng. 104, 321-328.   DOI
39 Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.   DOI
40 Unaogu, I.C., Gugnani, H.C., and Lacey, J. 1994. Occurrence of thermophilic actinomycetes in natural substrates in Nigeria. Antonie van Leeuwenhoek. 65, 1-5.   DOI
41 Yassin, A.F. 2009. Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma. Int. J. Syst. Evol. Microbiol. 59, 1148-1152.   DOI
42 Yu, H., Zeng, G., Huang, H., Xi, X., Wang, R., Huang, D., Huang, G., and Li, J. 2007. Microbial community succession and lignocellulose degradation during agricultural waste composting. Biodegradation 18, 793-802.   DOI