Browse > Article
http://dx.doi.org/10.4489/KJM.2013.41.2.85

Morphological Characteristics of Decomposition and Browning of Oak Sawdust Medium for Ground Bed Cultivation of Lentinula edodes  

Koo, Chang-Duck (Department of Forest Science, Chungbuk National University)
Lee, Seon-Jeong (Department of Environmental Science and Ecological Engineering, Korea University)
Lee, Hwa-Yong (Department of Forest Science, Chungbuk National University)
Publication Information
The Korean Journal of Mycology / v.41, no.2, 2013 , pp. 85-90 More about this Journal
Abstract
This study investigated the internal and external morphological characteristics of decomposition and browning of oak sawdust medium for ground bed cultivation of Lentinula edodes. Within fifty days after L. edodes inoculation, surface hyphae on the bed browned. In 110 days, the fungal hyphae occupied and decomposed wood fibers, vessels and parenchymatous cells from the inside as white profuse hyphal mass was amorphously dissolving the saw dust particles from the outer surface. Most of the white hyphal bed surface became cleanly brown, however, some colony surface became blackened and slimy with contaminating bacteria, hyphae and spores. The brown layer was ca. 0.34 mm thick with highly dense and white hyphal mass beneath, whereas the blackened layer was ca. 1.17 mm thick with shrunken hyphae and less decomposed sawdust particles beneath. The surface hardness of the brown surface was ca. $0.73kgf/cm^2$, soft and resilient, while that of the blackened was ca. $0.91kgf/cm^2$, hard and nonresilient. By 150 days Lentinula edodes mushrooms fruited only on the brown surface and not on the blackened medium.
Keywords
Browning of sawdust cultivation media; Characteristics of saw dust decomposition; Ground bed cultivation of Lentinula edodes;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Park, Y. A., Lee, K. T., Bak, W. C., Kim, M. K., Ka, K. H. and Koo, C. D. 2011. Eritadenin contents analysis in various strains of Lentinula edodes using LC-MS/MS. Kor. J. Mycol. 39:239-242. (in Korea).   과학기술학회마을   DOI   ScienceOn
2 Sato, T., Kanda, K., Okawa, K., Takahashi, M., Watanabe, H., Hirano, T., Yaegashi, K., Sakamoto, Y. and Uchimiya, H. 2009. The tyrosinase-encoding gene of Lentinula edodes, Letyr, is abundantly expressed in the gills of the fruit-body during post harvest preservation. Biosci. Biotechnol. Biochem. 73:1042-1047.   DOI   ScienceOn
3 Savoie, J. M., Mata, G. and Billette, C. 1998. Extracellular laccase production during hyphal interactions between Tricholoma sp. and shiitake, Lentinula edodes. Appl. Microbiol. Biotechnol. 49:589-593.   DOI   ScienceOn
4 Stamets, P. 2000. Growing gourmet and medicinal mushrooms. pp. 574. Ten Speed Press, Berkeley.
5 Wilcox, W. W. 1968. Change in wood microstructure through propressive stages of decay. pp. 46. USDA For. Prod. Lab. Res. Paper FPL 70.
6 Wilcox, W. W. 1973. Degradation in relation to wood structure. In: Wood degradation and its preservation by preservative treatments, Vol. : Degradation and preservation of wood, pp. 107-148. Eds. D. D. Nicholas. Syracuse Univ. Press, New York.
7 Yoo, Y. B., Koo, C. D., Kim, S. H. Seo, G. S., Shin H. D., Lee, J. W., Lee, C. S. and Jang, H. Y. 2010. Mushroom Science. Nature and Human Being. Seoul, pp. 453. (in Korean).
8 Yu, L. 2012. Present development situation and tendency of edible mushrooms industry in China. Mushroom Science X VIII:3-9. Proceedings of the 18th Congress of the International Society for Mushroom Science. Edited by J. Zhang, H. Wang and M. Chen. China Agriculture Press, Beijing.
9 Forest Mushroom Research Institute. 2009. Oak Mushroom Cultivation Technology. pp. 230. Forest Cooperatives, Gyeonggido. (in Korean).
10 Kim, Y. H., Jhune, C. S., Park, S. C., You, C. H., Sung, J. M. and Kong, W. S. 2009. The changes in intracellular enzyme during the mycelial browning of Lentinula edodes (Berlekey) Sing. J. Mushroom Science and Production 7:110-114. (in Korean).
11 Kim, Y. H., Jhune, C. S., Park, S. C., You, C. H., Sung, J. M. and Kong, W. S. 2011. Cultural characteristics on collected strains of Lentinula edodes and correlation with mycelial browning. J. Mushroom Science and Production 9:145-154. (in Korean).
12 Kim, Y. H., Park, S. C., Jhune, C. S., You, C. H., Sung, J. M. and Kong, W. S. 2012. BCR (Brown Color repressor) gene isolation related to mycelial browning of Lentinula edodes. J. Mushroom Science and Production 10:120-128. (in Korean).
13 Kim, Y. H., You, C. H., Sung, J. M. and Kong, W. S. 2007. Enzymatic activities related mycelial browning of Lentinula edodes (Berkeley) Sing. J. Mushroom Science and Production 5:91-97. (in Korean).
14 Mao, X. L. 2000. The Macrofungi in China. pp. 719. Hanam Science and Technology Publication Ltd. Jungju. (in Chinese).
15 Park, W. C., Yoon, G. H., Kim, S. C. and Hong, G. S. 2008. New cultivation technology for sustainable production of Lentinula edodes. pp. 307. National Forest Research Institute. Seoul. (in Korean).
16 Park, W. H. and Lee, H. D. 1999. Illustrated Book of Korean Medicinal Mushrooms. pp. 758. Kyohak Publishing Co. Seoul. (in Korean).