• 한국환경과학회지
• /
• 제20권9호
• /
• pp.1205-1212
• /
• 2011

As serving people's activities, pedestrian road systems are basic and necessary facilities in urban structure. To provide and utilize these pedestrian road systems in residential area would enhance urban environment as well as quality of life. For this reason, pedestrian road should be planned by consideration of people's activity in residential area. Evaluation of existing pedestrian road should be also oriented how people use it and what people do in it. This study amis to investigate functions of pedestrian road system throughout evaluation of user's satisfaction in order to improve better pedestrian road system in residential area. The purposes of this study are to analyze components of factors affecting on user's satisfaction, and to find the relationship among affecting factors. For this study, the on-site questionnaire method was applied to 267 individuals who were collected as the study areas where locate Toyogaoka and Kaidori, Japan. The collected data were clarified exploratory factors, and analyzed relationship between the factors and satisfaction by applying quantitative statistical techniques for the mapping investigation, Mann-Whitney u-test, and correlation. The results of this study are follows. The pedestrian road system is more preferred than surroundings of vehicle roads in residential district area where maintains pedestrian road as open space. In addition, satisfactions of the pedestrian road for each purpose were highly evaluated, because of conformability and convenience for usage. Consequently, the pedestrian road which is secure and greening as an open space is well carried out for the living circulation of residents. It would suggest that pedestrian road have to be managed and planned not a function of circulation but an open space system.

• 한국가정과교육학회지
• /
• 제23권2호
• /
• pp.131-148
• /
• 2011

이 연구의 목적은 2007년 개정 교육과정 중학교 기술 가정 교과서 주생활 영역의 교육내용을 분석하여 차기 기술 가정 교과서 개발 및 교사의 교육내용 재구성 시 기초자료로 제공하고자 하였다. 분석대상은 2011년 적용된 중학교 2학년 기술 가정 교과서 11종 전부를 대상으로 하였다. 연구결과, 주생활 영역은 교과서마다 분량의 차이가 있었으나 각 단원별 용어, 표/그림, 읽기자료 활동자료 등이 풍부하여 실천 문제 중심의 수업에 도움을 주는 것으로 파악되었으며 새롭게 추가된 근린환경과 친환경적 주생활 등은 2007년 개정 교육과정의 내용을 잘 반영하고 있는 것으로 평가되었다. 앞으로의 교과서 개발에는 중요한 현안인 저출산, 고령화와 녹색생활의 관점이 반영되어야 할 것이며 지역사회와의 긴밀한 네트워크로 환경 문제에 적극적으로 대처할 수 있는 교육 내용이 강조될 필요가 있다.

• Journal of Plant Biotechnology
• /
• 제1권1호
• /
• pp.46-55
• /
• 1999

The expressed sequence tags(ESTs) from immature seed of rice, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by homology. As of 1998, the partial sequences of about 6,600 cDNA clones were analyzed from normal and normalized immature seed cDNA libraries. About 2,200 ESTs were putatively identified by BLASTX deduced amino acid sequence homology analysis. About 20% of them were putatively identified as storage proteins. Also the clones were highly homologous to genes involved particularly in starch biosynthesis, glycolysis, signal transduction and defenses. Compared to 35% of redundancy in the ESTs of normal cDNA library, that from the substracted library was 15%. The Korea Rice Genome Network is maintained to provide the updated information of sequences, their homologies and sequence alignments of ESTs. For the stable expression of transgene in rice, diverse vectors were developed for overexpression, targeting and gene dosage effect with transit peptides (Tp) and matrix attachment region (MAR) sequence from chicken lysozyme locus. The rice calli were transformed via Agrobacterium tumefaciens LBA4404(pSB1) with the triparental mating technique and selected by herbicide resistance. The green fluorescent protein(GFP) gene in expression vector under the control of rbcS promoter-Tp was overexpressed upto 10 % of the total soluble protein. In addition, the Tp-sGFP fusion protein was properly processed during translocation into chloroplast. The expression of sGFP in the presence of MAR sequences was analyzed with Northern and immunoblot analysis. All the lines in which sGFP transgene with MAR sequence, showed position independent and copy number-dependent expression, while the lines without MAR showed the varied level of expression with the integration site. Thus the MAR sequence significantly reduced the variation in transgene expression between independent transformants.

• ALGAE
• /
• 제18권3호
• /
• pp.217-223
• /
• 2003

Seasonal growth and maturation process of Caulerpa okamurae were investigated in natural populations and in culture. Monthly sampling was carried out by SCUBA diving at Baekunpo, Busan, southeastern coast of Korea from November 1999 to October 2001. Growth of erect branches depended mainly on the water temperature in the natural habitat. Maximum length of erect branches was 14.0 $\pm$ 1.4 cm in June 2001 when the water temperature was 19.7$^{\circ}C$ and minimum was 2.8$\pm$0.2 cm in April when the water temperature was 14.7$^{\circ}C$. Fresh weight of erect branches was 3.9 $\pm$ 0.5 g in June 2001 and 0.2 $\pm$ 0.04 g in September 2000. Biomass of the population was maximum of 922.5 g dw${\cdot}m^{-2}$ in July and minimum of 125.6 g dw${\cdot}m^{-2}$ in April. Gametangial network was observed on the ramuli when the water temperature was over 19$^{\circ}C$ in August 2000 and June 2001. In the culture regime of 4 temperatures (15, 20, 25 and 30$^{\circ}C$) and 3 irradiances (20, 50 and 80 $\mu$mol${\cdot}m^{-2}{\cdot}s^{-1}$) combination, the maturation of excised erect branches was mainly affected by temperature. Maturation was induced under all irradiance conditions at 20 and 25$^{\circ}C$; under 80 $\mu$mol${\cdot}m^{-2}{\cdot}s^{-1}$ at 15$^{\circ}C$; and under 20 $\mu$mol${\cdot}m^{-2}{\cdot}s^{-1}$ at 30$^{\circ}C$. The highest rate of maturation was 64% under 20 $\mu$mol${\cdot}m^{-2}{\cdot}s^{-1}$ at 25$^{\circ}C$. These results suggested that developmental initiations of C. okamurae occurred at higher than 13$^{\circ}C$ and maturation took about 270 degree-days.

• 농촌계획
• /
• 제22권1호
• /
• pp.1-11
• /
• 2016

The analysis of research trends is used to identify key subject areas, research trends over time, and new discussions in a study field. The purpose of this study is to examine the characteristics of research articles published in the area of rural planning using bibliometric methods. Research articles published in the Journal of Korean Society of Rural Planning "Rural Planning" from 1995 to 2014 were analyzed to determine the annual publications, the number of periodical contributions, the authorship patterns, and popular research subjects and keywords. The results of this study are as follows; (1) The number of research papers and authors has increased over the 20 years periods (1995-2014); (2) The most published authors are associated with the study of rural planning. Seoul National University, Rural Development Administration, and Chonnam National University have highly contributed to the paper publications; (3) The most frequent word in titles is "Rural" for the whole period from 1995 to 2014. The most frequent words are "Village" and "Planning" from 1995-2006, and "Landscape" and "Village" in 2007-2010, and "Village" and "Area" from 2011-2014; and (4) The most used keywords are "Rural amenity", "Rural village", and "Rural landscape" for all of the different time frames.

• 대한교통학회지
• /
• 제20권5호
• /
• pp.81-98
• /
• 2002

본 연구의 목적은 매 주기마다 교차로에 출입하는 차량의 도착률을 근거로 다음 주기의 대기차량 길이와 평균지체도를 예측하여 대기차량길이와 평균지체도를 최소화하는 이중목적 변동주기 기반의 동적 신호시간 결정모형을 개발하고 유전자 알고리즘을 이용하여 신호 최적화 모형을 구축하는데 있다. 본 논문에 적용된 주기변동기반 시스템은 기존의 고정주기, 고정 현시값을 적용해서는 실제 현장에서 교통상황의 변화에 적응하기 어렵기 때문에 매 주기마다 교통수요에 근거하여 주기길이와 현시길이를 조정하여 교차로 교통신호운영을 최적화 하는 것이다. 또한 목적함수 값으로 평균지체도와 평균대기차량을 동시에 적용함으로써 교차로 막힘이나 타 교차로의 신호운영에 악영향을 미치치 않으면서 최적의 신호운영을 가능하게 하는 것이다. 본 모형의 적용결과 비포화상태나 포화상태의 경우 모두 TRANSYT-7F나 PASSER-II보다는 동적으로 신호를 계산하는 본 모형이 대기차량수와 통과차량수, 지체도에 있어서 더 좋은 결과를 나타내었다. 그리고 이중목적함수를 적용한 결과 두 가지 목적함수값에서 서로 상쇄(trade-off)가 되며 양자를 모두 고려할 수 있는 현시길이와 주기길이를 찾을 수 있다는 것을 보여주었다. 본 연구결과 지능형 교통체계로서 실시간 신호최적화에 유용하게 적용될 수 있는 첨단교통신호 모형으로 주기변동기반 및 다중목적 신호최적화모형의 적용 가능성을 보여준 것으로 판단된다.

• 한국ITS학회 논문지
• /
• 제9권6호
• /
• pp.83-93
• /
• 2010

회전교차로는 신호교차로보다 대형 교통사고, 혼잡비용 및 온실가스 효과를 줄이는 녹색교통체계이다. 본 연구의 목적은 다지 회전교차로의 운영효율을 분석하는데 있다. 이를 위해 본 연구에서는 다지 회전교차로와 신호교차로의 최적의 기하구조를 구축하고, 주도로와 부도로의 진입교통량의 비율을 반영한 분석시나리오를 작성하며, VISSIM을 이용하여 차량당 평균제어지체를 비교 분석하는데 중점을 두고 있다. 연구의 주요 결과는 다음과 같다. 첫째, 총 진입 교통량이 3,500pcph 이하일 때, 5지 1차로와 2차로 모두 회전교차로가 신호교차로보다 효과적으로 분석된다. 둘째, 6지 1차로 교차로일 경우 총 진입교통량이 3,600pcph이하에서 회전교차로가 신호교차로에 비해 효과적이다. 6지 2차로 교차로의 경우엔 회전교차로가 모든 진입교통량 조건에서 신호교차로보다 효과적으로 분석된다.

• IMMUNE NETWORK
• /
• 제8권3호
• /
• pp.98-105
• /
• 2008

Background: Allergic inflammation was induced by activated Th2 lymphocytes, leading to IgE production and eosinophil activation. A Th2 disproportion was shown in atopic children soon after birth. During specific allergen stimulation, an increase of Th2 cells was observed in most cases. In this study, we prepared new screening "whole blood" system for searching the anti-atopic materials. Cytokine production and IgE secretion from whole blood system were assessed and we confirmed the results by using animal system. Methods: Pathological features in NC/Nga mice are similar to those observed in human atopic dermatitis. Whole blood from NC/Nga mouse was stimulated by using TNCB (Th2 activator) or candidate materials of anti-atopic dermatitis, and the production of cytokines (IL-4, IL-12, and IFN-${\gamma}$) were measured by ELISA. In order to confirm the results of whole blood system, in vivo test was done by using NC/Nga mice. Results: In whole blood system, LPS and extracts of green tea, hardy orange and onion induced the production of IL-12 and IFN-${\gamma}$ while they reduced the production of IL-4. Also, LPS and extracts of onion reduced IgE production. Though atopic dermatitis was observed from a mouse stimulated with TNCB, it was not when a mouse was co-stimulated in LPS or extracts of onion. The results are same as those observed in whole blood system. Conclusion: Whole blood system was simple and speedy methods for searching a materials compared with the conventional high-cost animal system. And the results using whole blood system was proved to be reliable in our experiments for screening anti-atopic material. We expect that the system can be applied to other experiments for searching similar materials.

• 한국지리정보학회지
• /
• 제10권4호
• /
• pp.22-34
• /
• 2007

우리나라는 그 동안 국토를 이용하고 관리함에 있어 생태적 계획에 입각한 지속가능한 보전 및 관리보다는 효율성과 경제성을 중심으로 한 개발지향적 논리에 초점을 맞춤으로써 국토 환경보전에 커다란 기여를 하고 있는 농경지 및 산지면적이 지속적으로 감소하고 있는 추세이다. 지역생태계의 가치에 대한 생태학적 인식이 부족한 가운데 이루어진 일련의 지속적인 개발압력은 자연환경의 자정능력을 초과하는 환경압력으로 작용함에 따라 지역생태계의 건전한 물질순환과 에너지 흐름에 부정적인 결과를 초래하고 있다. 이에 본 연구에서는 기존의 물환경 문제해결을 위한 공간단위로서의 유역개념이 아닌 생태계를 평가하고 관리할 수 있는 공간 단위개념으로서 유역을 분석하기 위하여 다양한 경관요소가 혼재하고 있는 낙동강 유역권을 대상으로 경관구성 요소 중 토지이용을 중심으로 공간분석기법을 활용하여 서로 유사한 패턴을 보이는 유형으로 분류하고, 각 유형별 지역에 대한 특징을 분석하였다.

• Journal of Microbiology and Biotechnology
• /
• 제26권12호
• /
• pp.2051-2059
• /
• 2016

To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was $3,219{\pm}755Hz$, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.