• 제목/요약/키워드: Green grape

검색결과 49건 처리시간 0.028초

고성능액체크로마토그래피를 이용한 농산물 중 Mandipropamid의 잔류분석법 확립 (Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry)

  • 권찬혁;장문익;임무혁;최훈;정다이;이수찬;유진영;이영득;이종옥;홍무기
    • 분석과학
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    • 제21권6호
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    • pp.518-525
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    • 2008
  • 포도, 고추, 배추, 토마토 및 감자 등 5종 농산물에 대하여 mandelamide계 신규 살균제인 mandipropamid의 잔류분석법을 확립하였다. 각 농산물을 세절한 시료에 methanol을 가하고 고속마쇄하여 잔류분을 추출하였다. 추출액을 포화식염수와 증류수로 희석하고 dichloromethane으로 분배하여 극성분순물을 제거하였으며, Florisil 흡착크로마토그래피법으로 추가 정제하였다. Mandipropamid의 HPLC/UV 검출기상 최소검출량은 2 ng (S/N>10, 0.01 AUFS)이었고 시료추출물 중 불순물에 의한 간섭은 관찰되지 않았다. 무처리 포도, 토마토, 고추, 배추 및 감자에 대하여 2수준 3반복으로 회수율을 산출한 결과 각각 $99.8{\pm}1.7$ (n=6), $89.3{\pm}5.3$(n=6), $98.7{\pm}2.2$ (n=6), $99.7{\pm}6.8$ (n=6) 및 $91.1{\pm}3.1$ (n=6)이었으며, 분석법의 정량한계는 0.02~0.04 mg/kg이었다. 아울러 검출된 잔류분은 재확인을 위하여 selected-ion monitoring (SIM)을 이용한 LC/MS법을 추가로 확립하였다. 본 연구에서 확립된 mandipropamid의 잔류분석법은 검출감도 및 재현성을 고려할 때 공정분석법으로 사용이 가능하였다.

천연식물성분이 아질산염 소거에 미치는 영향 (Effect of Natural Plant Components on the Nitrite-scavenging)

  • 이수정;정미자;신정혜;성낙주
    • 한국식품위생안전성학회지
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    • 제15권2호
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    • pp.88-94
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    • 2000
  • 차류(녹차, 두충), 약용식물류(어성초, 삼백초, 백화사설초), 해조류(김, 미역, 청각), 채소류(피망, 케일, 오이, 양파) 및 과실류(토마토, 매실. 자두, 포도)추출물로부터 pH를 달리한 in vitro반응계에서 비타민 C함량과 아질산염 소거작용을 분석하였다. 비타민 C 함량은 녹차추출물에서 65.1∼77.1 mg/100 g로 가장 높았으며, 채소류는 과실류보다 대체로 높게 정량되었다. 아질산염 소거작용은 pH 1.2에서 그 효과가 가장 우수하였으며 차류, 약용식물류 및 해조류의 경우 pH 1.2에서 각각 57.0∼100%, 50.0∼100% 및 18.0∼99.0%였다. 특히 케일 쥬스 10 m1 및 매실쥬스 5ml첨가시에도 100%까지 아질산염 소거작용을 나타내었다.

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녹차 종실유의 제조법에 따른 열산화 안정성 비교 (Effects of Extraction Method on the Thermal Oxidative Stability of Seed Oils from Camellia sinensis L.)

  • 김미선;이재환;김명애
    • 한국식생활문화학회지
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    • 제25권6호
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    • pp.788-794
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    • 2010
  • Camellia sinensis L. (green tea) seed oils were prepared by roasting at $213^{\circ}C$ and pressing (RP), pressing (P), and nhexane extraction (H). The physico-chemical properties of the RP, P, and H samples, including fatty acid composition, color, and sensory characteristics were analyzed. RP, P and H samples were thermally oxidized at $180^{\circ}C$, and oxidative stability was determined by DPPH, CDA, and p-AV at 0, 20, 40, 60, and 80 min. Compared to the P and H samples, RP resulted in significantly higher thermal oxidative stability according to the DPPH, CDA, and p-AV results (p<0.05). The ratio of unsaturated fatty acids to saturated fatty acids among RP, P, and H samples were significantly different (p<0.05). The oleic acid and linoleic acid contents in green tea seed oils were 58 and 23%, respectively. Hunter's color value of lightness (L) for the RP, P, and H samples was not significant. Redness (a) of RP was $3.47{\pm}0.119$ and yellowness (b) of H was $60.10{\pm}2.483$, which were significantly different. Compared to RP samples, H and P samples had the highest color and off-odor values in the sensory evaluation. RP samples showed the highest taste value and were significant overall (p<0.05). The thermal stability of RP extraction was more stable than any other method. Camellia sinensis L. seed oil extracted by RP had better sensory characteristics than other edible oils, including soybean oil, grape seed oil, and extra virgin olive oil.

Kiwi-persimmon wine produced using wild Saccharomyces cerevisiae strains with sugar, acid, and alcohol tolerance

  • Hee Yul Lee;Kye Man Cho;Ok Soo Joo
    • 한국식품저장유통학회지
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    • 제30권1호
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    • pp.52-64
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    • 2023
  • 100 different yeast colonies were isolated from spontaneously fermented kiwis, persimmons, apples, pears, watermelons, grapes, grape fruits, peachs, and plums, and selected yeast strains were used to produce kiwi-persimmon mixed wine (KPMW). Among the isolates, five representative strains exhibited tolerance to sucrose, alcohol, pH, and potassium metabisulfite when compared with the control yeast strain (Saccharomyces cerevisiae KCCM 12615). All five yeast strains (Y4, Y10, Y28, Y78, and Y81) exhibited 99% 26S rDNA sequence similarity to S. cerevisiae. The pH, acidity, Brix, reducing sugar, alcohol, and organic acid contents were consistent in KPMW prepared from the S. cerevisiae KCCM 12615 and Y28 strains. KPMW made from the Y4, Y10, and Y28 strains exhibited lower quantities of free sugars than those of the KPMW made from the other yeast strains. The level of ethyl esters in KPMW prepared from the Y28 was higher than that in the other KPMWs. All strains, except for Y28, produced lower concentrations of sulfur and ketone compounds. Furthermore, the KPMW produced by the Y28 strains had total phenolic contents with 1.1 g/L, with DPPH and ABTS radical scavenging activities of 57.06% and 55.62%, respectively, and a FRAP assay value of 0.72. Our results suggest that Y28 is a promising yeast strain for producing high-quality wines.

Food allergy knowledge, perception of food allergy labeling, and level of dietary practice: A comparison between children with and without food allergy experience

  • Choi, Yongmi;Ju, Seyoung;Chang, Hyeja
    • Nutrition Research and Practice
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    • 제9권1호
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    • pp.92-98
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    • 2015
  • BACKGROUND/OBJECTIVES: The prevalence of food allergies in Korean children aged 6 to 12 years increased from 10.9% in 1995 to 12.6% in 2012 according to nationwide population studies. Treatment for food allergies is avoidance of allergenic-related foods and epinephrine auto-injector (EPI) for accidental allergic reactions. This study compared knowledge and perception of food allergy labeling and dietary practices of students. SUBJECTS/METHODS: The study was conducted with the fourth to sixth grade students from an elementary school in Yongin. A total of 437 response rate (95%) questionnaires were collected and statistically analyzed. RESULTS: The prevalence of food allergy among respondents was 19.7%, and the most common food allergy-related symptoms were urticaria, followed by itching, vomiting and nausea. Food allergens, other than 12 statutory food allergens, included cheese, cucumber, kiwi, melon, clam, green tea, walnut, grape, apricot and pineapple. Children with and without food allergy experience had a similar level of knowledge on food allergies. Children with food allergy experience thought that food allergy-related labeling on school menus was not clear or informative. CONCLUSION: To understand food allergies and prevent allergic reactions to school foodservice among children, schools must provide more concrete and customized food allergy education.

포도 캠벨얼리의 무가온 하우스재배시 지역별 갈색무늬병 발생차이 (Regional Differences of Leaf Spot Disease on Grapevine cv. 'Campbell Early' Caused by Pseudocercospora vitis in Plastic Green House)

  • 정성민;박종한;박서준;이한찬;이재욱;류명상
    • 식물병연구
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    • 제15권3호
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    • pp.193-197
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    • 2009
  • 포도갈색무늬병은 주로 포도 캠벨얼리에서 6월 상순에 발생이 시작하여 장마철을 지나면서 급속히 전파되는 중요한 병중 하나이다. Pseudocercospora vitis에 의해 발생되는 갈색무늬병의 발생소장을 김제, 김천에 소재한 무가온 하우스에서 2007년에 조사하였다. 병의 발생율은 노지, 무가온 하우스에 따라 매우 다르게 나타났고 지역간의 차이는 나타나지 않았다. 무가온 하우스내의 온도와 상대습도와 같은 미기상요인을 대상으로 갈색무늬병 이 병엽률과의 연관성을 SAS의 PROC REG방법을 사용하여 통계분석 하였다. 그 결과 미기상 요인과 병발생간에 Y(Infected leaves)=-7.0101+0.0496$\times$20Hcum+0.0208$\times$20cum-0.2781$\times$25Hcum ($R^2=0.95^{**}$)과 같은 회귀식이 도출되었고 이는 특정 이상 온도 및 시간의 누적값이 무가온하우스 작형에서 Pseudocercospora vitis에 의한 포도 갈색무늬병에 있어 중요인자임을 보여준다.

국내유통 식품중 total bromide의 잔류량 (Market survey on total bromide residues in foods)

  • 박건상;홍무기;최동미;오창환;황인균;박종세
    • 농약과학회지
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    • 제2권1호
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    • pp.65-69
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    • 1998
  • 식품중 총 브롬의 함유량을 2-bromoethanol로서 조사하기 위하여 국내산 농산물인 쌀, 보리, 당근, 오이, 사과, 토마토, 호박, 피망, 멜론, 딸기, 포도, 복숭아, 감자 및 셀러리를 GC/ECD와 GC/MSD로 분석하였다. 대부분의 국내산 농산물에서 브롬이 검출되었으며, 보리에서 최고수준인 13.2 ppm이 검출되었다. 또한 멜론, 키위, 레몬, 파인애플, 바나나, 오렌지, 포도 등 수입 농산물에 대하여 브롬함유량을 조사한 결과 모든 시료에서 브롬이 검출되었으며, 파인애플에서 검출된 12.8 ppm이 수입농산물 중 최고 검출량으로 나타났다. 라면스프에 대한 브롬의 잔류량을 최근 4년간 조사한 결과, 1994년에는 검출범위 $ND{\sim}2.4$ ppm (평균 1.1 ppm)로 조사대상시료 24건중 22건에서 검 출되었고, 1995년은 1.0, 2.2 ppm이 조사대상시료 37건중 2건에서 검출되었으며; 1996년에는 검출범위는 $0.7{\sim}37$ ppm (평균 12.4 ppm)로 조사대상시료 11건에서 모두 검출되었으며; 1997년도에는 검출범위는 $0.2{\sim}37ppm$ (평균 1.2ppm)로 조사대상시료 59건에서 모두 검출되었다. 이 연구대상 식품중 검출된 브롬잔류량이 우리나라 및 각 국의 브롬잔류허용기준을 초과한 경우는 없었으며, 또한 식품 중 브롬의 잔류에 대한 조사결과는 외국산보다 우리나라산이 대체로 낮은 수준이었다. 한편 이 연구에서 가장 높은 잔류량인 스프류의 37 ppm에 대한 위해도를 평가한 결과 브롬 1일섭취허용량의 0.2%에 해당하는 양이었다.

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국산 시판 로제 와인의 품질 특성 (Quality Characteristics of Korean Domestic Commercial Rosé Wines)

  • 박정미;박혜진;정창원;최원일;김시동;윤향식
    • 한국식품영양학회지
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    • 제30권5호
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    • pp.889-899
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    • 2017
  • To investigate the quality characteristics of domestic $ros{\acute{e}}$ wines marketed in Korea, 11 kinds of wines were purchased at the Korea Wine Festival in 2016. The color, physiological activity, aroma component and sensory evaluations were conducted. The hue value of $ros{\acute{e}}$ wines ranged from 0.592 to 1.990, with the Ro7 of Delaware having the highest value. The brightness of $ros{\acute{e}}$ wines ranged from 42.96 to 94.99, the redness from 3.20 to 59.37, and the yellowness from 8.43 to 24.83. Of the 11 $ros{\acute{e}}$ wines evaluated, 1 was a dry wine and 10 were sweet wines. The average sugar content of the sweet wines was 73.89 mg/mL. Among the organic acid contents, malic acid ranged from 0.214 to 2.903 mg/mL, and lactic acid content ranged from 0 to 3.423 mg/mL. Malolactic fermentation differed, depending on the source. The total polyphenol content of $ros{\acute{e}}$ wines ranged from 50.55 to 99.55 mg%, the anthocyanin content was 2.12 to 213.30 mg/L, and the DPPH radical scavenging activity of $ros{\acute{e}}$ wines was between 73.75 to 90.41%. A total of 41 volatile components were detected, including 7 alcohols, 22 esters, 4 terpenes, 3 acids and 5 other compounds. Of these, 9 compounds had odor activity value(OAV) greater than 1; these were identified as 1-propanol (alcohol, pungent), 3-methyl-1-butanol (harsh, nail polish), ethyl butanoate (strawberry, lactic), isopentyl acetate (fresh, banana), ethyl hexanoate (green apple, fruity), ethyl octanoate (pineapple, pear, floral), ethyl decanoate (fruity, fatty, pleasant), linalool (flower, lavender) and limonene (lemone, orange). As a result of the sensory evaluation, the Ro5 wine made from Campbell Early grape, and having a good color, flavor and taste, was the most preferred.

포도 '거봉' 품종에 있어서 착립 정도와 과실 품질과의 관계 (Relationship between Berry Set Density and Fruit Quality in 'Kyoho' Grape)

  • 박서준;김진국;정성민;노정호;허윤영;류명상;이한찬
    • 원예과학기술지
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    • 제28권6호
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    • pp.954-958
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    • 2010
  • 포도 '거봉' 품종의 적정 착과량 설정을 위한 기초자료를 마련하고자 신초당 착립수를 조절하여 변색기 이후의 과실의 품질변화를 조사하였다. 과실의 당도는 변색기 이후 30일까지 급격히 증가하였으며, 신초당 착립수가 적을수록 빠르게 증가하였다. 신초당 10립과 20립 처리구의 당도는 변색기 이후 4주까지 $18.0^{\circ}Bx$ 이상 급격히 상승한 다음, 완만하게 증가하였다. 신초당 30립 처리구의 당도는 변색초기에는 느리게 상승하였으나, 수확기까지 지속적으로 상승하여 $18.0^{\circ}Bx$에 도달하였다. 그러나 신초당 40립과 50립 처리구는 변색기 이후 4주까지도 당도의 상승폭이 현저히 적었고, 성숙기에 도달해도 $15.0^{\circ}Bx$에 그쳤다. 과즙의 산 함량은 모든 처리구에서 변색기 이후 30일까지 빠르게 감소하였고, 그 이후에는 완만하게 감소하였으며, 신초당 50립 처리구를 제외한 모든 처리구의 산 함량은 0.4-0.6%에 도달하였다. 과피색은 변색기 이후 모든 처리구에서 성숙이 진행됨에 따라 녹색에서 자흑색으로 변화하면서 L, b값은 감소하였고, a값은 증가하였다. 총 안토시아닌 함량은 신초당 10립과 20립 처리구의 경우 변색기 이후 2주까지 완만히 증가한 후 변색기 이후 6주까지 급격히 증가하였고, 그 후에는 감소하는 양상을 나타내었으며, 신초당 30, 40 및 50개의 착립구는 정상적인 착색을 나타내지 못하였다. 따라서 본 연구결과 과실품질을 고려할 때 거봉 포도의 착립 정도는 신초당 20립 내외가 적당한 것으로 나타났다.

Gallic acid가 Lipopolysaccharide로 활성화된 마우스 대식세포의 인터루킨 생성에 미치는 영향 (Inhibitory Effect of Gallic acid on Production of Interleukins in Mouse Macrophage Stimulated by Lipopolysaccharide)

  • 박완수
    • 대한약침학회지
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    • 제13권3호
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    • pp.63-71
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    • 2010
  • Objectives: Gallic acid (GA) is the major component of tannin which could be easily founded in various natural materials such as green tea, red tea, grape juice, and Corni Fructus. The purpose of this study is to investigate the effect of Gallic acid (GA) on production of interleukin (IL) in mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods: Productions of interleukins were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. Firstly, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, it was incubated with the antibody-conjugated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System and concentration of interleukin was determined. Results: The results of the experiment are as follows. 1. GA significantly inhibited the production of IL-3, IL-10, IL-12p40, and IL-17 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). 2. GA significantly inhibited the production of IL-6 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). 3. GA diminished the production of some cytokine such as IL-4, IL-5, and IL-13 in LPS-induced mouse macrophage RAW 264.7 cells. 4. GA did not show the inhibitory effect on the production of IL-$1{\alpha}$ and IL-9 in LPS-induced mouse macrophage RAW 264.7 cells. Conclusions: These results suggest that GA has anti-inflammatory activity related with its inhibitory effects on the production of interleukins such as IL-3, IL-10, IL-12p40, IL-17, and IL-6 in LPS-induced macrophages.