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http://dx.doi.org/10.3831/KPI.2010.13.3.063

Inhibitory Effect of Gallic acid on Production of Interleukins in Mouse Macrophage Stimulated by Lipopolysaccharide  

Park, Wan-Su (Dept. of Pathology, College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Pharmacopuncture / v.13, no.3, 2010 , pp. 63-71 More about this Journal
Abstract
Objectives: Gallic acid (GA) is the major component of tannin which could be easily founded in various natural materials such as green tea, red tea, grape juice, and Corni Fructus. The purpose of this study is to investigate the effect of Gallic acid (GA) on production of interleukin (IL) in mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods: Productions of interleukins were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$ (multi-analyte profiling beads) technology. Firstly, cell culture supernatant was obtained after treatment with LPS and GA for 24 hour. Then, it was incubated with the antibody-conjugated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. And Strepavidin-conjugated Phycoerythrin (SAPE) was added. After incubation for 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System and concentration of interleukin was determined. Results: The results of the experiment are as follows. 1. GA significantly inhibited the production of IL-3, IL-10, IL-12p40, and IL-17 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 25, 50, 100, 200 uM (p<0.05). 2. GA significantly inhibited the production of IL-6 in LPS-induced mouse macrophage RAW 264.7 cells at the concentration of 50, 100, 200 uM (p<0.05). 3. GA diminished the production of some cytokine such as IL-4, IL-5, and IL-13 in LPS-induced mouse macrophage RAW 264.7 cells. 4. GA did not show the inhibitory effect on the production of IL-$1{\alpha}$ and IL-9 in LPS-induced mouse macrophage RAW 264.7 cells. Conclusions: These results suggest that GA has anti-inflammatory activity related with its inhibitory effects on the production of interleukins such as IL-3, IL-10, IL-12p40, IL-17, and IL-6 in LPS-induced macrophages.
Keywords
Gallic acid; Macrophage; Interleukin; Inflammation; lipopolysaccharide;
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1 Negi AS, Darokar MP, Chattopadhyay SK, Garg A, Bhattacharya AK, Srivastava V, Khanuja PS. Synthesis of a growth promoter from gallic acid. Bioorg Medic Chem Let. 2005; 15: 1243-1247.   DOI   ScienceOn
2 Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P. Prostaglandin E receptor type 4-associated protein interacts directly with NF-kappaB1 and attenuates macrophage activation. J Biol Chem. 2008; 283: 9692-9703.   DOI   ScienceOn
3 Luo Y, Liu M, Yao X, Xia Y, Dai Y, Chou G, Wang Z. Total alkaloids from Radix Linderae prevent the production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells by suppressing NF-kappaB and MAPKs activation. Cytokine. 2009; 46(1): 104-110.   DOI   ScienceOn
4 Lin YL, Tsai SH, Lin-Shiau SY, Ho CT, Lin JK. Theaflavin-3,3'-digallate from black tea blocks the nitric oxide synthase by down-regulating the activation of NF-kappaB in macrophages. Eur J Pharmacol. 1999; 367(2-3): 379-388.   DOI   ScienceOn
5 Kim OK. Antidiabetic and antioxidative effects of Corni fructus in streptozotocininduced diabetic rats. J Korean Oil Chem Soc. 2005; 22: 157-167.   과학기술학회마을
6 Park YK, Whang WK, Kin HI. The antidiabetic effects of extract from Cornus officinalis seed. Chung-Ang J Pharm Sci. 1995; 9: 5-11.
7 주진우. 발효차중의 미량성분인 gallic acid 산화물 purpurogallin carboxylic acid의 항염증 효과. 한국식품과학회지. 2008; 40: 707-711.   과학기술학회마을
8 Ryan JG, Kastner DL. Fevers, genes, and innate immunity. Curr Top Microbiol Immunol. 2008; 321: 169-184.   DOI
9 Bennett MK, Kirk CJ. Development of proteasome inhibitors in oncology and autoimmune diseases. Curr Opin Drug Discov Devel. 2008; 11: 616-625.
10 Li L, Yi Z, Tisch R, Wang B. Immunotherapy of type 1 diabetes. Arch Immunol Ther Exp (Warsz). 2008; 56: 227-236.   DOI
11 Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009; 125(2): 286-290.   DOI   ScienceOn
12 Farasat S, Aksentijevich I, Toro JR. Autoinflammatory diseases: clinical and genetic advances. Arch Dermatol. 2008; 144: 392-402.   DOI
13 Danila MI, Hughes LB, Bridges SL. Pharmacogenetics of etanercept in rheumatoid arthritis. Pharmacogenomics. 2008; 9: 1011-1015.   DOI   ScienceOn
14 Boyer JF, Cantagrel A, Constantin A. Impact of traditional therapies and biologics on cardiovascular diseases in rheumatoid arthritis. Curr Vasc Pharmacol. 2008; 6: 218-227.   DOI   ScienceOn
15 Kim YH. Isolation of constituents from the fruits Cornus officinalis. Siebold. 1999; 14: 287-292.
16 Chung SR, Jeune KH, Park SY, Jang SJ. Toxicity and lectins constituents from the seed of Cornus officinalis. Korean J Pharmacogn. 1993; 24: 177-182.   과학기술학회마을
17 Lee JY. Iridoid glycosides of Cornus officinalis. MS thesis. Seoul National Univ, Seoul, Korea, 1981.
18 KI, Lee SW, Yang KH. Antimicrobial and antioxidative activities of Corni fructus extracts. Korean J Postharvest Sci Technol. 1999; 6: 99-103.   과학기술학회마을
19 Guilian T, Zhang T, Yang F, Ito Y. Separation of gallic acid from Cornus officinalis Sieb. et Zucc by high-speed counter-current chromatography. J Chromatogr A. 2000; 886: 309-312.   DOI   ScienceOn