• 한국재료학회지
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• 제9권1호
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• pp.46-50
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• 1999

액상반응법에 의해 $\textrm{Zn}_{2-x}\textrm{Mn}_{x}\textrm{SiO}_{4}$ 녹색 형광체를 합성한 후 소성온도($900^{\circ}C$-$1200^{\circ}C$) 및 Mn 활성제 농도(x=0.01~0.20)에 따른 발광특성과 결정특성을 조사하였다. 147nm와 254nm 여기원을 사용한 경우 형광체의 소성온도가 $900^{\circ}C$에서 $1200^{\circ}C$로 증가함에 따라 상대발광피크강도는 약 4배 이상 크게 증가하였다. XRD 분석결과 $1100^{\circ}C$이상의 소성온도에서 $\textrm{Zn}_{2}\textrm{SiO}_{4}$:Mn 녹색 형광체에서 나타나는 전형적인 willemite 결정구조를 보여주었다. $1200^{\circ}C$의 온도로 소성된 $\textrm{Zn}_{2-x}\textrm{Mn}_{x}\textrm{SiO}_{4}$형광체 시료의 경우 147nm 여기원에서 Mn 활성제 농도가 x=0.02에서 최대 발광강도를 나타내었으며 x=0.10 이상에서 발광강도가 급격히 저하하는 농도 칭 현상이 나타났다. SEM 분석결과 형광체 입자는 구형에 가까운 형상을 보여주었으며 $1200^{\circ}C$에서 소성된 형광체 입자크기는 약 2~3$\mu\textrm{m}$이었다.EX>이었다.

• 한국결정성장학회지
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• 제19권6호
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• pp.305-310
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• 2009

PMMA 입자의 표면에 양전하를 갖는 전해질 폴리머 PDDA와 음전하를 갖는TALH를 사용하여 $TiO_2$ 박막을 LBL 법에 의해 성공적으로 제조하였다. 수정진동자의 측정을 통해 TALH의 용액의 pH가 감소됨에 따라 TALH의 적층량이 늘어나고 PMMA의 입자 표면에 코팅된 (PDDA/TALH) 박막의 두께가 증가됨을 확인하였다. (PDDA/TALH)n의 순서에 의해 코팅된 PMMA 입자들은 bilayer 수의 변화에 따라 다양한 색 변화를 보여주었다. (PDDA/TALH) 박막의 bilayer 수(n)가 10과 20 일 경우에 $a^*$와 $b^*$의 값은 막이 코팅되지 않은 PMMA의 값보다 감소하였고 색 변화는 $a^*$, $b^*$ 색도도에서 각각 green과 blue 방향으로 이동하였다. 이후 n의 수가 30, 40으로 증가됨에 따라 $a^*$와 $b^*$의 값은 증가하였고 색의 변화는 red와 yellow 방향으로 각각 이동하였다. 최종적으로 $(PDDA/TALH)_{50}$ 박막이 코팅된 PMMA 입자들은 박막이 코팅되지 않은 PMMA 입자들과 거의 비슷한 $a^*$와 $b^*$의 값을 보여주었다.

• 한국해안해양공학회지
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• 제8권4호
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• pp.274-286
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• 1996

2차원 유연체해석 이론(수탄성 이론)을 여러개의 단위체 연결로 이루어진 거대 부체구조물의 해석에 적용하였다. 동수역학적 계수를 구하기 위해 경계요소법과 그린함수법이 사용되었으며 부체자체는 연직방향으로 파랑에 반응하는 연체보로 정수역학적 탄성계수에 대한 운동을 고려하여 운동방정식이 유도된다. 두가지 다른 형식의 연결, 즉 회전강성을 가진 것과 강성을 무시한 핀 형식의 연결요소가 고려되며 반잠수한 부체에 대해 해석결과가 제시된다. 해석결과는 거대 부체구조물의 설계에 필요한 변위와 내력에 관한 개념을 제시한다. 또한 수치해석 결과에 따르면 부체의 움직임은 핀연결이 강성체 연결보다 더 크며 부체의 내력휨 응력은 강성체 연결에서 휠씬 더 크게 증가하였다.

• 한국자기공명학회논문지
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• 제19권2호
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• pp.67-73
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• 2015

The microcystin is a cyclic heptapeptide from metabolites of cyanobacteria in the genera mycrocystis, anabaeba as a result of eutrophication. It has been known that microcystin-LR is a potent inhibitor of the catalytic subunits of protein phosphatase-1 (PP-1) as well as powerful tumor promoter. The active site of microcystin actually has two metal ions $Fe^{2+}/Zn^{2+}$ close to the nucleophilic portion of PP-1-microcystin complex. We report the isolation and purification of this microcystin-LR from cyanobacteria (blue-green algae) obtained from Daechung Dam in Chung-cheong Do, Korea. Microcystin-LR was extracted from solid-phase extraction (SPE) sample preparation using a CN cartridge. The cyanobacteria extract was purified to obtain microcystin-LR by HPLC method and identified by LC/MS. The detail structural studies that can elucidate the possible role of monovalent and divalent metal ions in PP-1-microcystin complexation were carried out by utilizing molecular dynamics. Conformational changes in metal binding for ligands were monitored by molecular dynamic computation and potential of mean force (PMF) using the method of the free energy perturbation. The microcystin-metal binding PMF simulation results exhibit that microcystin can have very stable binding free energy of -10.95 kcal/mol by adopting the $Mg^{2+}$ ion at broad geometrical distribution of $0.5{\sim}4.5{\AA}$, and show that the $K^+$ ion can form a stable metal complex rather than other monovalent alkali metal ions.

• 한국환경복원기술학회지
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• 제9권3호
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• pp.26-38
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• 2006

For both a healthy environment and sustainable development, we frame a habitat assessment method, concerning biodiversity and physical characteristics. With the method, we set up and extract conservation areas from Peace Valley Resort Development Areas which can function as habitats. Absolute and relative assessment items which are the size of area, habitation of species with conservation value, distribution of communities, functions of habitats, ratio of non-native plants, connectivity of habitats, vegetation layers of forests and age of forests, and water systems are considered on newly extracted conservation areas, and the habitat value assessment was calculated on the items in a 3-point scale. By comparing and examining 3 existing proposals for extracted conservation areas, we assess existing proposals, make an alternative proposal, and try to verify the possibility of applicability to extracted conservation areas. Proposal A and C must not be adopted since almost all conservation areas would be developed in the proposals. Proposal B, consisting of 53% development areas and 47% non-development areas, does not have enough development areas, so high density building arrangements should be encouraged in order to preserve conservation areas. Therefore, proposal B would be the best in ecological terms among the 3 proposals, if 3 conditions are considered : 1) new extracted conservation areas should be conserved; 2) 10~15m wide green-zones on both sides of the water system, which can play a role as ecological corridors, should be considered in development areas; 3) building arrangement should not interrupt the ecological corridors.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.77-80
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• 2001

A variety of different methods to generate diverse proteins, including random mutagenesis and recombination, are currently available, and most of them accumulate the mutations on the target gene of a protein, whose sequence space remains unchanged. On the other hand, a pool of diverse genes, which is generated by random insertions, deletions, and exchange of the homologous domains with different lengths in the target gene, would present the protein lineages resulting in new fitness landscapes. Here we report a method to generate a pool of protein variants with different sequence spaces by employing green fluorescent protein (GFP) as a model protein. This process, designated functional salvage screen (FSS), comprises the following procedures： a defective GFP template expressing no fluorescence is firstly constructed by genetically disrupting a predetermined region(s) of the protein, and a library of GFP variants is generated from the defective template by incorporating the randomly fragmented genomic DNA from E. coli into the defined region(s) of the target gene, followed by screening of the functionally salvaged, fluorescence-emitting GFPs. Two approaches, sequence-directed and PCR-coupled methods, were attempted to generate the library of GFP variants with new sequences derived from the genomic segments of E. coli. The functionally salvaged GFPs were selected and analyzed in terms of the sequence space and functional property. The results demonstrate that the functional salvage process not only can be a simple and effective method to create protein lineages with new sequence spaces, but also can be useful in elucidating the involvement of a specific region(s) or domain(s) in the structure and function of protein.

• 한국농공학회논문집
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• 제62권1호
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• pp.17-27
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• 2020

The objective of this study was to assess the flood probability based on temporal distribution of forecasted-rainfall in Cheongmicheon watershed. In this study, 6-hr rainfalls were disaggregated into hourly rainfall using the Multiplicative Random Cascade (MRC) model, which is a stochastic rainfall time disaggregation model and it was repeated 100 times to make 100 rainfalls for each storm event. The watershed runoff was estimated using the Clark unit hydrograph method with disaggregated rainfall and watershed characteristics. Using the peak discharges of the simulated hydrographs, the probability distribution was determined and parameters were estimated. Using the parameters, the probability density function is shown and the flood probability is calculated by comparing with the design flood of Cheongmicheon watershed. The flood probability results differed for various values of rainfall and rainfall duration. In addition, the flood probability calculated in this study was compared with the actual flood damage in Cheongmicheon watershed (R² = 0.7). Further, this study results could be used for flood forecasting.

• 한국재료학회지
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• 제22권7호
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• pp.329-334
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• 2012

Two types of nanoclusters, termed Cluster (1) and Cluster (2) here, both play an important role in the age-hardening behavior in Al-Mg-Si alloys. Small amounts of additions of Cu and Ag affect the formation of nanoclusters. Two exothermic peaks were clearly detected in differential scanning calorimetry(DSC) curves by means of peak separation by the Gaussian method in the base, Cu-added, Ag-added and Cu-Ag-added Al-Mg-Si alloys. The formation of nanoclusters in the initial stage of natural aging was suppressed in the Ag-added and Cu-Ag-added alloys, while the formation of nanoclusters was enhanced at an aging time longer than 259.2 ks(3 days) of natural aging with the addition Cu and Ag. The formation of nanoclusters while aging at $100^{\circ}C$ was accelerated in the Cu-added, Ag-added and Cu-Ag-added alloys due to the attractive interaction between the Cu and Ag atoms and the Mg atoms. The influence of additions of Cu and Ag on the clustering behavior during low-temperature aging was well characterized based on the interaction energies among solute atoms and on vacancies derived from the first-principle calculation of the full-potential Korrinaga-Kohn-Rostoker(FPKKR)-Green function method. The effects of low Cu and Ag additions on the formation of nanoclusters were also discussed based on the age-hardening phenomena.

• 대한조선학회논문집
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• 제38권3호
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• pp.41-46
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• 2001

최근에 선박이 대형화되는 추세에 힘입어 조선소는 광폭천흘수선, 초대형 원유운반선 및 초대형 컨테이너선 등을 건조하고 있다. 이와 같은 선박은 상대적으로 다른 선박에 비해 강성이 작기 때문에 파랑 중에서 유탄성 운동을 하게 되고, 입사하는 파고가 작은 경우에도 선체의 2절 모드의 진동에 의해 선체의 갑판이 피로 파괴되는 경우가 종종 발생하는 것으로 알려져 있다. 본 논문에서 전진하는 선박의 유체 압력을 계산하기 위해 적분방정식은 3차원 소오스 분포법을 사용하고, 그린함수는 전진하면서 동요하는 형태를 이용하였다. 방사문제는 선박을 여러 개의 단면으로 나누어 단면간의 간섭효과를 고려하여 heave 및 pitch 강제동요와 관련된 부가질량 및 조파 감쇠계수를 계산하였고, 파강제력은 각 단면에서 선행해에 의한 힘만 고려하였다. 선박의 각 단면의 수직운동은 선박에 대한 운동방정식을 이용하고 강성행렬은 오일러 보 이론에 의해 산정되었다. 계산은 Esso-Osaka 선박을 모델로 도입하여 입사하는 파도의 주파수가 변함에 따른 선박의 각 단면에 대한 운동, 굽힘 모우멘트를 계산하였다.

• Geomechanics and Engineering
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• 제19권1호
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• pp.11-20
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• 2019

The use of electrokinetic dissipation method to study the fluid flow law in micro-pores is of great significance to reservoir rock microfluidics. In this paper, the micro-capillary theory was combined with the coupling model of the seepage field and the current field under the excitation of the harmonic signal, and the coupling theory of the electrokinetic effect under the first-order approximation condition was derived. The dissipation equation of electrokinetic dissipation and viscous resistance dissipation and its solution were established by using Green's function method. The physical and mathematical models for the electrokinetic dissipation of reservoir rocks were constructed. The microscopic mechanism of the electrokinetic dissipation of reservoir rock were theoretically clarified. The influencing factors of the electrokinetic dissipation frequency of the reservoir rock were analyzed quantitatively. The results show that the electrokinetic effect transforms the fluid flow profile in the pores of the reservoir from parabolic to wavy; under low-frequency conditions, the apparent viscosity coefficient is greater that one and is basically unchanged. The apparent viscosity coefficient gradually approaches 1 as the frequency increases further. The viscous resistance dissipation is two orders of magnitude higher than the electrokinetic effect dissipation. When the concentration of the electrolyte exceeds 0.1mol/L, the electrokinetic dissipation can be neglected, while for the electrolyte solution (<$10^{-2}M$) in low concentration, the electrokinetic dissipation is very significant and cannot be ignored.