• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.249-256
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• 1992

Italian ryegrass (Lolium multiflorum Lam.) was fractionated into leaf nutrient concentrate, fiber and deproteinised juice (DPJ). The fresh and fermented DPJs were concentrated and referred to as fresh deproteinised juice concentrate (FDPJC) and fermented deprotenised juice concentrate (FMTD DPJC). The FDPJC and FMTD DPJC were separately mixed with dried fiber and ensiled. Wilted crop silage and fresh fiber silage were also prepared from the same material crop. The nutritive value of these four silages were compared using four goats by $4{\times}4$ Latin square design. Green crop fractionation resulted lesser amount of crude protein and ash, and higher amount of neurtal detergent fiber, acid detergent fiber, cellulose and hemicellulose in fresh fiber. The pH of fresh fiber silage was lower than that of the other silages. Addition of FDPJC or FMTD DPJC to the dried fiber at ensiling did not improve the silage qualities; but all the silages were satisfactorily preserved. Goats fed these silages showed similar ruminal pH and total volatile fatty acid concentrations. But addition of FMTD DPJC was effective on increasing ruminal acetic acid concentration reducing propionic acid concentration. Ruminal n- and iso- butyric acid concentrations were proportional to that of propionic acid. High ammonia content of the silage containing FMTD DPJC was reflected to the ruminal ammonia concentration, urinary nitrogen excretion and serum urea level of goats. Inclusion of FDPJC or FMTD DPJC added 15 to 25% dry matter to the fiber silages with a little reduction in the digestibilities of most components of the silages.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.5
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• pp.435-441
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• 2010

To verify the possibility of a power generation system using biogas from the waste of pig farm for rural electric production, a SI gasoline engine is modified to use biogas fuel and was installed in a 20 KVA power generation system. An electronic speed regulation unit is developed to keep the system speed at 1500 rpm. Experimental investigations have been carried out to examine the performance characteristics of power generation system (such as: system frequency, phase output voltage,$\ldots$). In addition, the operating parameters and output emissions ($NO_x$, HC, and $CO_2$) of biogas-fueled engine are preliminary evaluated and analyzed for the change of system load. Results indicated that the researched power generation system shows a high stability of output voltage and frequency with help of speed regulator. Biogas fuel (mainly $CH_4$ and $CO_2$) has an environmental impact and potential as a green alternative fuel for SI engine and they would not require significant modification of existing engine hardware. Output emissions of biogas-fueled engine are found to be relative low. $NO_x$ emission increases with the increase of output electric power of the power generation system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.12
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• pp.1611-1617
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• 2018

The body temperature of livestock is directly related to the health of livestock such that it changes immediately when there exists health problem. Accordingly, the monitoring of livestock's temperature is one of most important tasks in farm management. However, the temperature of livestock is usually measured using skin-attached sensor which is significantly affected by the outside temperature and the condition of attachment which results in the inaccurate measurement of temperature. Herein we have proposed new scheme which estimates the body core temperature of cow based on measured data from neck-attached smart sensor. Especially, we have considered both schemes which estimate the exact temperature and which detect the unusually high temperature based on machine learning. We have found that the occurrence of high temperature can be detected accurately. The proposed scheme can be used in monitoring of health condition of cow and improving the efficiency of farm management.

• Journal of Korean Society of Rural Planning
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• v.28 no.1
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• pp.1-15
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• 2022

A Care farm is known to have positive effects on various people in psychological, physical, and social aspects. However, care farm services for the disabled, transportation disadvantaged, and socially disadvantaged are limited. This study conducted a demand survey in order to provide basic data for development of the Agro-Healing Virtual Reality Therapy(AVRT) system. The respondents were the ordinary person(n=127) and the disabled person(n=72), and the survey items consisted of 4 categories and 20 items, including intention to use AVRT, requirements for use, factors to be considered when developing the system, and content and program preferences. The intention to use a AVRT was found to be at a high level of 80% or more by respondent characteristics. In addition, similar results were shown in all items such as virtual reality experience, willingness to use, appropriate use time, and willingness to pay by respondent characteristics, and correlation by item was determined through correlation analysis. As for the conditions of use, both sides preferred rural types and were found to have the purpose of healing. However, there were concerns about dizziness in common between system use, and in the case of the disabled person, there were difficulties in purchasing expensive equipment. In the development of the AVRT system, the part of sensory priority, important technology level, and color preference were evaluated, and the preference of content and programs to be developed in the system was identified. Fruit, vegetables, flowers, and animals preferred mandarin, tomato, tulips, and dogs first, horticultural healing preferred harvest management for the ordinary person, plant cultivation for the disabled person, and forest healing and animal education preferred walking and dog-related programs. However, agricultural work was found to be a program with high preference for making processed foods for the ordinary person and creating an animal breeding environment for the disabled person. The result of this study is expected to provide reference data that can be suggested for the development of Agro-healing Virtual Reality Therapy system.

• Journal of the Korean Institute of Landscape Architecture
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• v.30 no.2
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• pp.70-78
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• 2002

The landscape disign of Gamcheon wholesale fish Market was designed around a turnkey base to promote the economy of Busan Metropolitan City, to establish a fishery marketing center and to modernize existing facilities. The objectives of the project were to promote the mood of an old market, while preserving its functions and efficiency as a market, to design outdoor spaces with natural resources and amenities in balance, and to create attractive tourist spots in connection with the wide area development plan. The project was oriented, fast, to enhance the functions of the market. For this purpose, a multi-dimensional space layout was designed in consideration of functions as a wholesale market. The safety of pedestrians was secured by separating lathes for vehicles and for pedestrians. Tree planting with various functions such as sheltering, wind breaking and guiding was planned. Secondly, nature-friendly and human-friendly landscaping design was attempted. For this, the beautiful natural resources of Amnam Park were utilized, and green spaces such as green bridges linking buildings in the wholesale market, and rooftop gardens were to be arranged. In addition, environment-friendly facilities such as roads paved with natural materials(i.e. gravel, shells) and program parking lots were to be planned. Thirdly, landscape design was considered to create attractive tourist spots. For example, a fish farm was created as a theme street for pedestrians and various water-friendly spaces such as pedestrian ramps, observatories and seaside streets were to be secured. The main contents are as follows. First, a green bridge to Ahnnam Park was introduced for a tour source and flower garden, an event plan and viewing deck open to the sea were planned on the bridge's axis. Secondly, for the effective land use plan concerning open space and convenience to visitors, a promenade was planned, which is connected with the theme plaza and small plazas by environmental sculptures in front of the market hall and at the gate. As well, an observatory and a roof garden help create three dimensional multi leveled space, with a good view of the natural landscape of the sea, sky and park Thirdly, landscape materials, such as trees and those for facilities, strengthened for protection against the seawind and salt damage were selected. The commercial market area was intended to be transformed a traditional functional area of efficiency and economy into an attractive marine leisure area where both tourists and neighbors can make use of it.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.1
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• pp.40-48
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• 2014

Due to increasing greenhouse gas emissions, global warming and abnormal weather phenomena it has become important on a national level to keep a count of greenhouse gases being emitted. We want to take advantage of any selected area, as the basic data for the calculation of greenhouse gas emissions, Forest and Grassland, Paddy fields, and Fields(crops), Greenhouse(crops), Pig farm, Cattle farm, Farm household(populations, agricultural machinery) and Vehicle, the basic building blocks shots with a small amount of per-unit basis, the statistics calculated based on regional carbon emissions through the literature and experimental. Carbon absorption 772,960 ton C/year, amount of fixation 487,477 ton C/year, amount of emission 1,112,607 ton C/year were noted in Gimje-si, and amount of carbon absorption 55,559 ton C/year, amount of fixation 25,864 ton C/year, amount of emissions 58,355 ton C/year in Gongdeok-myeon, respectively. The carbon absorption at Hwangsan-ri is 25,107 ton C/year, fixation 4,301 ton C/year, and emission 20,330 ton C/year respectively. We were able to estimate the amount of carbon according to the specific characteristics of each unit village, then expanding it to a large-scale and comparative analysis, therefore we were able to obtain basic data on the national levels of carbon absorption.

• Journal of Navigation and Port Research
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• v.38 no.2
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• pp.105-110
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• 2014

Korean government sets up a goal that jumps up to the third ranked powerful nation of offshore wind in the world until 2020 and announced "The plan for 2.5-gigawatt wind farm off the south-west coast by 2019". Such above, according to green energy policy, offshore wind farms(OWF) will be increased continuously. The development of OWF should be taken account of wind volume as well as marine traffic environment. Specially aids to navigation of OWF play a significant role in preventing collision between vessels navigating near waters and structures. For purpose of distinguishing OWF, IALA recommendations define installation of lights on SPS and IPS. However, there is no mention of light character that plays important role in identification of lights as marking offshore wind farm. Also the research on selection of proper light character has been insufficient state. Therefore in this paper, we analyzed internal and external regulations concerned marking with light on SPS and IPS in OWF. And suggested patterns and rhythms of light having not only easily recognized feature but also no confusion with other light of aids to navigation. The proposed light characters were verified by simulation, and the results were analysed that synchronism flickering of "Fl Y(4) 12s(SPS)" and "Fl Y 6s(IPS)" would be useful in combination of both lights.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1529-1540
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• 2011

Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is relatively nil. In the present study, we have validated the interfering effects of siRNA (small interfering RNA) using both quantitative and qualitative gene silencing in buffalo granulosa cells. Qualitative gene knockdown was validated using a fluorescent vector, enhanced green fluorescence protein (EGFP) and fluorescently labeled siRNA (Cy3) duplex. While quantitatively, siRNA targeted against the luciferase and CYP19 mRNA was used to validate the technique. CYP19 gene, a candidate fertility gene, was selected as a model to demonstrate the technique optimization. However, to sustain the expression of CYP19 gene in culture conditions using serum is difficult because granulosa cells have the tendency to luteinize in presence of serum. Therefore, serum free culture conditions were optimized for transfection and were found to be more suitable for the maintenance of CYP19 gene transcripts in comparison to culture conditions with serum. Decline in fluorescence intensity of green fluorescent protein (EGFP) was observed following co-transfection with plasmid generating siRNA targeted against EGFP gene. Quantitative decrease in luminescence was seen when co-transfected with siRNA against the luciferase gene. A significant suppressive effect on the mRNA levels of CYP19 gene at 100 nM siRNA concentration was observed. Also, measurement of estradiol levels using ELISA (enzyme-linked immunosorbent assay) showed a significant decline in comparison to control. In conclusion, the present study validated gene silencing using RNAi in cultured buffalo granulosa cells which can be used as an effective tool for functional analysis of target genes.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.396-401
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• 2006

The objective of this study was to investigate the antitumor activity of suspension cultures of tea callus cells grown in the presence of different concentrations of the growth regulator 2,4-dichlorophenoxy acetic acid (2,4-D) with or without light irradiation. The methanol and ethanol extracts of precipitated cells (MEP, EEP) exhibited stronger inhibitory effects on the growth of tumor cell lines than the water extract of precipitated cells (WEP) or the supernatant Compared to culture under dark conditions, exposure to light irradiation led to significantly higher antitumor activity. The MEP from light irradiated cells at $250{\mu}g/mL$ with 2.0mg/L 2,4-D displayed more than 64% growth inhibition of HEP-2 cells, whereas normal cells showed less than 25% growth inhibition. The some fractions of MEP obtained from Diaion HP-20 column chromatography displayed the majority of inhibitory activity against the HEP-2 cell line. These results show that 2,4-D, and light stimulated the synthesis of antitumor compounds.

• Journal of Navigation and Port Research
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• v.37 no.6
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• pp.655-662
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• 2013

Recently, by the problems owing to utilization of fossil fuel, various green energies receive attention. Wind, the impetus for the wind power generation as one of the green energies, is observed higher quality value in the offshore than onshore. Also, the development of offshore wind turbines is in the spotlight as alternative to solve the problems of onshore wind farm such as securing sites, noise, and electromagnetic waves, and to get efficient wind energy. Therefore, the many researches on offshore wind energy have been carried out. As wind towers are advanced to ocean, offshore wind towers have been enlarged. Thus, stability is required to endure wind force and wave force. In this study, the external forces act on the foundation in multi-layered are calculated by p-y relation.