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Antitumor Activity of Cell Suspension Culture of Green Tea Seed (Camellia sinensis L.)  

Choi, Jae-Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Yoon, Sang-Kun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Lee, Keyong-Ho (Kolon Central Research Park)
Seo, Min-Soo (Hankooktea Company, Honam Tea Farm)
Kim, Doo-Hwan (Department of Molecular Biotechnology, Konkuk University)
Hong, Seung-Beom (Department of Molecular Biotechnology, Konkuk University)
Kim, Ji-Yeon (Department of Animal Science, Woosong Information College)
Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Kim, Chang-Han (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.5, 2006 , pp. 396-401 More about this Journal
Abstract
The objective of this study was to investigate the antitumor activity of suspension cultures of tea callus cells grown in the presence of different concentrations of the growth regulator 2,4-dichlorophenoxy acetic acid (2,4-D) with or without light irradiation. The methanol and ethanol extracts of precipitated cells (MEP, EEP) exhibited stronger inhibitory effects on the growth of tumor cell lines than the water extract of precipitated cells (WEP) or the supernatant Compared to culture under dark conditions, exposure to light irradiation led to significantly higher antitumor activity. The MEP from light irradiated cells at $250{\mu}g/mL$ with 2.0mg/L 2,4-D displayed more than 64% growth inhibition of HEP-2 cells, whereas normal cells showed less than 25% growth inhibition. The some fractions of MEP obtained from Diaion HP-20 column chromatography displayed the majority of inhibitory activity against the HEP-2 cell line. These results show that 2,4-D, and light stimulated the synthesis of antitumor compounds.
Keywords
Camellia sinensis L.; green tea seed; cell suspension culture; secondary metabolite; antitumor activity; MTT assay;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 10  (Related Records In Web of Science)
Times Cited By SCOPUS : 9
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1 Furuya, T., Y. Origara, and Y. Tsuda (1990) Caffeine and theanine from cultured cells of Camellia sinensis. Phytochemistry 29: 2539-2543   DOI   ScienceOn
2 Millin, D. J. (1987) Factors affecting quality of tea. pp. 127-160. In: S. M. Herschduerter (eds.). Quality Control in the Food Industry. Academic Press, London, England
3 Yang, C. S., J. Y. Chung, G. Y. Yang, S. K. Chhabra, and M. J. Lee (2000) Tea and tea polyphenols in cancer prevention. J. Nutr. 130: 472S-478S
4 Matsuura, T. and T. Kakuda (1990) Effects of precursor temperature and illumination on theanine accumulation in tea callus. Agric. Biol. Chem. 54: 2283-2286
5 Sur, P. and D. K. Ganguly (1994) Tea plant root extract (TRE) as an antineoplastic agent. Planta Med. 60: 106-109   DOI   ScienceOn
6 Das, M., P. Sur, A. Gomes, J. R. Vedasiromoni, and D. K. Ganguly (2002) Inhibition of tumor growth and inflammation by consumption of tea. Phytother. Res. 16: 40-44   DOI   ScienceOn
7 Prakash, G., C. J. S. K. Emmannuel, and A. K. Srivastava (2005) Variability of azadirachtin in Azadirachta indica (neem) and batch kinetics studies of cell suspension culture. Biotechnol. Bioprocess Eng. 10: 198-204   과학기술학회마을   DOI   ScienceOn
8 Calzada, F., M. Meckes, and R. Cedillo-River (1999) An-tiamoebic and antigardial activity of plant flavonoids. Planta Med. 65: 78-80   DOI   ScienceOn
9 Li, X. C., H. N. ElSohly, A. C. Nimrod, and A. M. Clark (1999) Antifungal activity of (-)-epigallocatechin gallate from Coccoloba dugandiana. Planta Med. 65: 780   DOI
10 Chung, S. K., M. Y. Kim, Y. C. Kim, K. Iwai, and H. Matsue (2004) Antioxidant effects of Korean teabag teas by a simple and fast XYZ-dish method. Food Sci. Biotechnol. 13: 197-201
11 Cao, Y. and R. Cao (1999) Angiogenesis inhibited by drinking tea. Nature 398: 381   DOI   ScienceOn
12 Ogutuga, D. B. A. and D. H. Northcote (1970) Caffeine formation in tea callus tissue. J. Exp. Bot. 21: 258-273   DOI
13 Shibasaki-Kitakawa, N., J. Takeishi, and T. Yonemoto (2003) Improvement of catechin productivity in suspension cultures of tea callus cells. Biotechnol. Prog. 19: 655-658   DOI   ScienceOn
14 Choi, D. B., W. S. Cha, S. H. Kang, and B. R. Lee (2004) Effect of Pleurotus ferulae extracts on viability of human lung cancer and cervical cancer cell lines. Biotechnol. Bioprocess Eng. 9: 356-361   DOI   ScienceOn
15 Chaudhuri, T., P. Sur, A. Gomes, S. K. Das, M. Das, and D. K. Ganguly (1998) Effect of tea root extract (TRE) on solid tumours induced by 3-methylcholanthrene in mice. Phytother. Res. 12: 62-64   DOI   ScienceOn
16 Yang, C. S., P. Maliakal, and X. Meng (2002) Inhibition of carcinogenesis by tea. Annu. Rev. Pharmacol. Toxicol. 42: 25-54   DOI   ScienceOn
17 Sakanaka, S., L. R. Juneja, and M. Taniguchi (2000) Antimicrobial effects of green tea polyphenols on thermophilic spore-forming bacteria. J. Biosci. Bioeng. 90: 81-85   DOI
18 Strekova, V Y., N. V. Zagoskina, G. A. Subbotina, and M. N. Zaprometev (1989) Effect of prolonged illumination on synthesis of phenolic compounds and chloroplast formation in tea callus tissues. Sov. Plant Physiol. 36: 65-71
19 Shervington, A., L. A. Shervington, F. Afifi, and M. A. El-Omari (1998) Caffeine and theobromine formation by tissue cultures of Camellia sinensis. Phytochemistry 47: 1535-1536   DOI   ScienceOn
20 Kuo, P. L. and C. C. Lin (2003) Green tea constituent (-) -epigallocatechin-3-gallate inhibits Hep G2 proliferation and induces apoptosis through p53-dependent and fas-mediated pathways. J. Biomed. Sci. 10: 219-227
21 Folin, O. and W. Denis (1912) On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12:239-243
22 Gupta, S., K. Hastak, N. Ahmad, J. S. Lewin, and H. Mukhtar (2001) Inhibition of prostate carcinogenesis in TRAMP mice by oral infusion of green tea polyphenols. Proc. Natl. Acad. Sci. USA 98: 10350-10355
23 Park, Y. G., S. J. Kim, H. Y. Jung, Y. M. Kang, S. M. Kang, D. T. Prasad, S. W. Kim, and M. S. Choi (2004) Variation of ginkgolides and bilobalide contents in leaves and cell cultures of Ginkgo biloba L. Biotechnol. Bioprocess Eng. 9: 35-40   DOI   ScienceOn
24 Bae, J. H. and J. E. Kim (2004) Inhibitory effect of Plantago asiatica extracts on the growth of gastric and colon cancer cell lines. Food Sci. Biotechnol. 13: 11-16
25 Mabe, K., M. Yamada, I. Oguni, and T. Takahashi (1999) In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob. Agents Chemother. 43: 1788-1791
26 Haider, B., S. Pramanick, S. Mukhopadhyay, and A. K. Giri (2005) Inhibition of benzo[a]pyrene induced mu-tagenicity and genotoxicity by black tea polyphenols theaflavins and thearubigins in multiple test systems. Food Chem. Toxicol. 43:591-597   DOI   ScienceOn
27 Takeo, T. (1974) L-Alanine as a precursor of ethylamine in Camellia sinensis. Phytochemistry 13: 1401-1406   DOI   ScienceOn
28 Yoon, W. H., J. H. Choi, K. H. Lee, and C. H. Kim (2005) Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Kor. J. Food Sci. Technol. 37: 108-112   과학기술학회마을