• Journal of the Korean Applied Science and Technology
• /
• v.2 no.1
• /
• pp.45-55
• /
• 1985

The present study was designed to investigate the effects of dietary fiber sources and levels on serum lipids in rats fed a hypercholesterol diet containing 0.5% cholesterol. Male Sprague-Dawley rats weighing 52.7 ${\pm}$ 7.27g were devided into sevel groups by completely randomized block design and fed various dietary fibers which were prepared from Rice bran, Siraegi (The dried green parts of a radish) and Bracken Fern at a level of 5% and 10% respectively. The results obtained in this study are summarized as follows: 1. Body weight gains were not significantly different among the groups and food intake was significantly higher in rats fed a diet of Siraegi 10% than those fed the control diet. 2. Fecal wet weights were significantly increased in all experimental groups. 3. Levels of serum total cholesterol in all experimental groups were significantly lower than that of the control group except the level in the R 10(Rice bran 10%) group. 4. Free Cholesterol levels in the serum were increased in all experimental groups, especially the levels in the R 10 (Rice bran 10%) and S 10 (Siraegi 10%) groups were significantly higher than that of the control group. 5. Ester Cholesterol levels in the serum were significantly lower in rats fed the experimental diets than those fed the control diet. 6. There was a tendency of higher serum HDL-Cholesterol levels in R5 (Rice bran 5%), R10 (Rice bran 10%) and B5(Bracken 5%) groups compared to the control group however no significant differences were found between the control group and all the experimental groups except in the case of the S5 (Siraegi 5%) group. 7. LDL, VLDL-Cholesterol levels in the serum sere significantly reduced in the S5 (Siraegi 5%), B5 (Bracken 5%) and B10 (Bracken 10%) groups. 8. The HDL-C./Total-C. Ratio was increased in all experimental group especially the Ratios in the S5 and B5 groups were significantly higher than that of the control group.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.19 no.1
• /
• pp.54-59
• /
• 2009

It was difficult for the existing rotary kiln to fabricate the fine aggregates under 3 mm due to the sticking phenomenon between specimens. In this study, the vertical type fluidizing furnace was designed and manufactured by which the lightweight fine aggregates of specific gravity $1.1{\sim}1.7$, water absorption $11{\sim}19%$ could be fabricated from the green body of clay: stone sludge: spent bleaching clay = 60 : 30 : 10 (wt%) without sticking-together happening. The minimum sintering temperature for bloating of aggregates was $1130^{\circ}C$. The specimens sintered over $1140^{\circ}C$ showed the typical bloating characteristics of lightweight aggregates and an inner layer was discovered due to widened cracks on a surface. But the crack on a surface did not propagate into a black core area so had no effect on a water absorption of aggregates. The sintering temperature made the thickness of shell and the black core area thin and expanded respectively but the sintering time did not affect the microsturcture of aggregates. The water absorption of aggregates decreased with increasing temperature owing to increased amount of liquid formed on a surface. Also sintering time affected a lot on a water absorption because it takes a time to form a liquid, which change the open pores to closed pores by blocking.

• Korean journal of applied entomology
• /
• v.42 no.1
• /
• pp.1-7
• /
• 2003

Morphological characteristics, developmental period, and seasonal occurrence of Adeia leucomelas (L.) were investigated from 1999 to 2000. In addition, consumption of sweetpotato as food was also examined. Adults of A. leucomelas were dark-brown and body lengths of females and males were 20.2 mm and 18.9 mm, respectively, Wing expanse of female and male was 33.7 mm and 29.4mm, respectively. Egg was flat round-shape. Larva was light yellow-green to dark-brown with 3.3-53.5 mm. Pupa was deep-brown and 15.1 mm in length. Developmental periods of A. leucomelas from egg to adult emergence at different temperatures of 15, 20, 25, and 30$^{\circ}$C were 108.5, 70.7, 40.2, and 29.1 days, respectively, Developmental threshold (DT) and effective accumulative temperatures were estimated as 10.7$^{\circ}$C and 67.5 DD in egg stage, 11.0$^{\circ}$C and 275.1 DD in larval stage and 9.3$^{\circ}$C and 244.6 DD in pupal stage, respectively. The longevity of adult female was shortened with increment of temperature, whereas the total numbers of eggs laid by a female were increased. The larvae of A.leucomelas occurred from mid-June to early October, and population reached its peak during early to mid-September in Jeonbuk province. Food consumption of A. leucomelas was highest at 20-25$^{\circ}$C. Food consumption of 1 st, 2nd, 3rd, 4th, and 5th larvae of A. leucomelas per day at 25$^{\circ}$C was 0.4, 3.6, 19.6, 40.7, and 78.9 $\textrm{cm}^2$, respectively.

• Journal of Life Science
• /
• v.14 no.5
• /
• pp.829-833
• /
• 2004

The major compositions of Lentinus edodes seasoning(LES) compose of 30.3% of Lentinus edodes fruit body, 18.1 % of anchovy (Engraulis japonicus) powder and 51.6% of sea tangle (Laminaria japonica), prawn and green tea powder and so on were investigated in order to evaluate the nutritional value. Sodium concentration in LES was high up to 5,042.99 mg/IOO g and K, Ca, P, Mg, Fe, Zn, Mn and Cu were followed. There were nineteen total amino acids in LES. The glutamic acid content was high up to 4.62 mg/100 mg and glycine, leucine, alanine, aspartic acid were followed. Among twenty free amino acids, glycine content was high up to 2.37 mg/100 mg and glutamic acid, asparanine, proline, taurin and aspartic acid were followed. The contents of vitamin C in LES was high up to 224.78 mg/100 g and pantothenic acid, vitamin B$_{6}$, niacin, vitamin E, folic acid, vitamin B$_{l}$ vitamin B$_{2}$, vitamin D$_{3}$, vitamin A, vitamin B$_{12}$ and vitamin $K_{l}$ were followed.wed.

• The Korean Journal of Malacology
• /
• v.15 no.1
• /
• pp.1-11
• /
• 1999

An immunohisochemical study on the cerebral ganglion of the African giant snail, Achatina fulica. was conducted by applying the AB/AY staining and the avidin-bovine-peroxidase complex staining methods. The followings are the results obtained throughout the study. The cerebral ganglion of Achatina fulica is an ellipsoidal body of 2 x 1 mm in size, which is connected by the cerebral commissure of 1 mm in diameter. The cross-section through the cerebral ganglion, shaped like a butterfly, is divided into the medio-dorsal parts, the latero-dorsal parts, the caudo-dorsal parts, and the lateral lobes. In the medio-dorsal and latero-dorsal parts, the LG cells and the DG cells are found mixed, although the LG cells are dominant. In lateral lobe, however, the Y cells are quite dominant, while the LG cells and the DG cells are seldom found. The LG cells are 20-70 $\mu\textrm{m}$ in sizes and circular or ellipsoidal in shapes. They are stained light green with the AB/AY. 1 - 3 nucleoli are found in karyolymph, where granular chromantins are evenly distributed. In cytoplasm, it is found that the secretory granules are evenly developed.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.12
• /
• pp.1732-1737
• /
• 2003

An experiment was conducted to investigate the effect of feeding transgenic cottonseed (Bt.) vis-a-vis non-transgenic (non-Bt.) cottonseed on blood biochemical constituents in lactating Murrah buffaloes. Twenty Murrah buffaloes in mid-lactation were divided into 2 groups of 10 each. Animals of group I were fed with 39.5% non-transgenic cottonseed in concentrate mixture while the same percentage of transgenic (Bt.) cottonseed was included in the concentrate mixture fed to the animals of group II. Animals of both groups were fed with concentrate mixture to support their milk production requirements. Each buffalo was also offered 20 kg mixed green fodder (oats and berseem) and wheat straw ad libitum. The experimental feeding trial lasted for 35 days. There was no significant difference in the dry matter intake between the two groups of buffaloes. All the buffaloes gained body weight, however, the differences were non significant. Total erythrocyte count, hemoglobin content and packed cell volume were $9.27{\pm}0.70${\times}10^6/{\mu}l$, $13.01{\pm}0.60gdl$ and $34.87{\pm}1.47%$, respectively in group I with the corresponding figures of $8.88{\pm}0.33$, $12.99{\pm}0.52$ and $31.08{\pm}1.52$ in group II. The values of total erythrocyte count, haemoglobin content and packed cell volume did not differ significantly between the two groups of buffaloes. The concentration of plasma glucose, serum total proteins, albumin, globulin, triglycerides and high density lipoprotein were non significantly higher in buffaloes fed non-transgenic cottonseed than in buffaloes fed transgenic cottonseed. The cholesterol concentration was significantly (p<0.01) higher in buffaloes of group I ($136.84{\pm}8.40mg/dl$) than in buffaloes of group II ($105.20{\pm}1.85mg/dl$). The serum alkaline phosphotase, glutamic-oxaloacetate transaminase and glutamic-pyruate transaminase activities did not differ significantly between two groups of buffaloes. However, serum glutamic-pyruate transaminase activity was considerably high in buffaloes fed nontransgenic cottonseed as compared to buffaloes fed transgenic cottonseed. Bt. proteins in serum samples of animals of group II were not detected after 35 days of feeding trial. It was concluded that transgenic cottonseed and non-transgenic cottonseed have similar nutritional value without any adverse effects on health status of buffaloes as assessed from haematobiochemical constituents.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.5
• /
• pp.661-666
• /
• 2005

The effects of pre-partum feeding management in terms of birth weight, growth, metabolic profile and immunity of calves were studied using 24 crossbred (Bos taurus${\times}$Bos indicus) cows, divided into three equal groups. The dietary treatments included feeding of either 3.0 kg concentrate/head/d throughout the 60 d pre-partum (T$_1$), or 3.0 kg concentrate during 60-22 d pre-partum and thereafter at an increased allowance at 0.25 kg/d during the next 21 d till it reached 1% of live weight (T$_2$). The third group of cows was fed similar to T$_2$, except that the concentrate feeding during 60-22 d pre-partum was reduced to 2.0 kg (T$_3$). All the groups had access to ad libitum green fodder throughout. The results revealed that the mean daily dry matter (DM) intake by the cows was similar (p>0.05) among the three groups during the 60 days of the pre-partum but T2 animals tended to gain more live weight (41.25 kg) than T$_1$ (38.12 kg) and T$_3$ (36.25 kg). The body condition score of the cows did not change appreciably over the experimental period. The mean birth weight of the calves was 24.00${\pm}$1.10, 24.63${\pm}$1.17 and 23.25${\pm}$1.19 kg for the three groups, respectively, with the corresponding average daily gain of 154.2, 155.0 and 169.7 g during the subsequent 60 days; both these parameters did not vary significantly ascribable to prepartum feeding regimens of their dams. The total immunoglobulin (Ig) concentration in the colostrum was 6.31${\pm}$0.34, 5.80${\pm}$0.21 and 6.13${\pm}$0.30 g/dl for the three groups, respectively, showing no influence of dietary treatments. The mean serum Ig levels (T$_1$ 2.10${\pm}$0.09, T$_2$ 2.05${\pm}$0.09 and T$_3$ 2.10${\pm}$0.12 g/dl) of calves at 5 d of age were similar among the dietary groups as was the case with various serum biochemical constituents. It is concluded that the variations in pre-partum dietary management elicited no significant influence on the calf performance including the immune status.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.9
• /
• pp.1420-1430
• /
• 2018

Objective: Epigallocatechin-3-gallate (EGCG) is a major ingredient of catechin polyphenols and is considered one of the most promising bioactive compounds in green tea because of its strong antioxidant properties. However, the protective role of EGCG in bovine oocyte in vitro maturation (IVM) has not been investigated. Therefore, we aimed to study the effects of EGCG on IVM of bovine oocytes. Methods: Bovine oocytes were treated with different concentrations of EGCG (0, 25, 50, 100, and $200{\mu}M$), and the nuclear and cytoplasmic maturation, cumulus cell expansion, intracellular reactive oxygen species (ROS) levels, total antioxidant capacity, the early apoptosis and the developmental competence of in vitro fertilized embryos were measured. The mRNA abundances of antioxidant genes (nuclear factor erythriod-2 related factor 2 [NRF2], superoxide dismutase 1 [SOD1], catalase [CAT], and glutathione peroxidase 4 [GPX4]) in matured bovine oocytes were also quantified. Results: Nuclear maturation which is characterized by first polar body extrusion, and cytoplasmic maturation characterized by peripheral and cortical distribution of cortical granules and homogeneous mitochondrial distribution were significantly improved in the $50{\mu}M$ EGCG-treated group compared with the control group. Adding $50{\mu}M$ EGCG to the maturation medium significantly increased the cumulus cell expansion index and upregulated the mRNA levels of cumulus cell expansion-related genes (hyaluronan synthase 2, tumor necrosis factor alpha induced protein 6, pentraxin 3, and prostaglandin 2). Both the intracellular ROS level and the early apoptotic rate of matured oocytes were significantly decreased in the $50{\mu}M$ EGCG group, and the total antioxidant ability was markedly enhanced. Additionally, both the cleavage and blastocyst rates were significantly higher in the $50{\mu}M$ EGCG-treated oocytes after in vitro fertilization than in the control oocytes. The mRNA abundance of NRF2, SOD1, CAT, and GPX4 were significantly increased in the $50{\mu}M$ EGCG-treated oocytes. Conclusion: In conclusion, $50{\mu}M$ EGCG can improve the bovine oocyte maturation, and the protective role of EGCG may be correlated with its antioxidative property.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.5
• /
• pp.699-707
• /
• 2002

Diets with foliage of Flemingia (Flemingia macrophylla) or Jackfruit (Artocapus heterophyllus were fed to goats with the objective to study nitrogen (N) balance and effect of a daily supplementation of polyethylene glycol (PEG) on intake and digestion. In experiment 1, three male Alpine${\times}$Jamnapary goats with initial weights varying from 26.9 to 27.7 kg were used in a $3{\times}3$ Latin square design in the dry season. Three Alpine${\times}$Bachthao crosses, 15.3-16.7 kg, were used in the same design in the wet season. The three diets were based on chopped whole sugar cane complemented with the two green foliages, Jackfruit and Flemingia, or soybean meal (SBM). The level of dry matter (DM) offered was 4% of body weight (BW), 2.7% as foliage and 1.3% as chopped whole sugar cane. The amount of SBM offered was calculated to give the same amount of crude protein (CP) as the foliages. Each experimental period lasted 32 days (14 days for adaptation, 7 days for collection and 10 days for rest). Feed intake, apparent digestibility of DM, organic matter (OM), CP, neutral detergent fiber (NDF) and acid detergent fiber (ADF) and retained nitrogen (N) were measured by total faecal and urine collection. In experiment 2, four male goats (Alpine${\times}$Jamnapary) with initial weights from 17.1 to 23.1 kg were used in a $4{\times}4$ Latin square design. The four treatments were Jackfruit or Flemingia with or without addition of PEG, which was fed at a level of 5 g/goat and day by mixing with a small amount of rice bran. Each experimental period lasted 15 days (8 days for adaptation, 7 days for collection). Measurements were done as in experiment 1. The DM digestibility was highest (65.9-74.3%) for goats fed the SBM diet in both the dry and wet season. The DM digestibility of goats fed the Jackfruit and the Flemingia diets was similar in both the dry (58.6-59.2% respectively) and the wet season (53.9-56.1% respectively). The CP digestibility was highest (73.0-73.6%) for the SBM diet followed by the Jackfruit diet (47.0-38.5%) and was lowest (36.8-30.0%) for the Flemingia diet in both dry and wet seasons, respectively. The NDF digestibility was low for both the Jackfruit (36.4%) and Flemingia (38.0%) diets in the wet season. All diets resulted in a positive N balance. The N retention was highest (0.465-0.604 g/kg $W^{0.75}$) in the SBM diets and lowest (0.012-0.250 g/kg $W^{0.75}$) in the Flemingia diet. Addition of PEG had no effect on feed intake for any of the diets. PEG added in the Flemingia diet had a positive effect only on NDF digestibility, but the digestibility of the Jackfruit diet was significantly increased. Supplementation with PEG reduced digestibility and N retention of Flemingia, possibly because of the low tannin level, but increased digestibility and N retention for Jackfruit foliage.

• Polymer(Korea)
• /
• v.24 no.1
• /
• pp.97-104
• /
• 2000

PAN-based activated carbon fibers/phenolic resin matrix composites (ACFCs) were manufactured via molding process with oxidized carbon fabrics (plain-type) and phenolic resin (resole-type) compounded by 70 : 30 wt%. The green body (as molded) was submitted to carbonization (at 100$0^{\circ}C$) in an inert environment and activation (at 700, 800, 900 and 100$0^{\circ}C$) in a $CO_2$ environment. In this work, the influence of activation temperatures was investigated in surface properties, such as pH, acid- and base-values by titration method, and in adsorption properties, i.e., specific surface area and pore structures by BET-method of the composites. Also, the pressure drops of the specimens were calibrated by ASTM. As a result, the activation temperature influenced the surface property of ACFCs. When the activation temperature was higher than 90$0^{\circ}C$, the surface was gradually developed in basic nature. And, the evolutions of specific surface area, total pore volume and pore size distribution of ACFCs could be easily confirmed the dependence on the activation temperature. Among them, well-developed pore structure from adsorption characteristics was changed of the ACFCs activated at 90$0^{\circ}C$. Also, the pressure drop was slightly decreased with increasing the temperature due to increasing the burn-off with heat treatment temperature of ACFCs.