• 한국방재학회 논문집
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• 제10권3호
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• pp.1-11
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• 2010

본 연구에서는 경기도 용인시 기흥구 구갈동(구갈역세권)과 보라구역, 중동구역 일대를 중심으로 개발계획, 개발잠재력, 입지여건과 아울러 녹색 및 재해예방 인프라에 대한 4가지 측면을 고려하여 대상지의 투자가치 평가를 통한 전략적 도시관리 방안을 연구하였다. 방법론 측면에서는 평가항목간의 상관관계를 고려할수 있는 네트워크 분석적 의사결정법을 통하여 판단해보고자 하였다. 분석 결과 개발계획에서는 개발용도, 키테넌트, 자금조달, 마케팅순으로, 개발잠재력에서는 시장성, 정체성, 역사성 순으로, 입지여건에서는 토지이용, 주변환경, 교통환경순으로, 마지막으로 녹색 및 재해예방인프라에서는 자연재해예방, 인적재해예방, 사회적 재해예방순으로 나타났다. 본 연구의 결과는 향후 도시개발사업 추진하는데 있어 합리적인 사전 평가방안으로 활용할 수 있을 것이라 본다.

• The Plant Pathology Journal
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• 제18권6호
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• pp.323-327
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• 2002

A rod-shaped virus was isolated from pepper showing mild mosic during the winter growing seasons of 2001 and 2002 in Korea. Based on its biological reactions, serological relationships, reverse transcription-poly-merase chain reaction (RT-PCR) using specific primers, and nucleotide sequence analysis of coat protein (CP) gene, the isolated virus was identified as Tobacco mild green mosaic virus (TMGMV) and designated as Korean pepper isolate (TMGMV-KP). Crude sap from infected tissue was mechanically transmitted to various indicator plants, which produced characteristic symptoms of tobamovirus infection. However, no symptom was observed in Gomphorena globosa. In RT-PCR assays with specific primers toy respective detection of TMGMV, Tobacco mosaic virus (TMV), Pepper mild mottle virue (PMMoV), and Tomato mosaic virus (ToMV), a single strong band of about 500 bp in length was produced from the sample used only with TMGMV primers. The amplified DNA was cloned and the nucleotide sequence was determined. Sequence comparisons with the CP gene of other tobamoviruses indicated that TMGMV-KP shared 99.3％ identity with TMGMV Japanese isolate and only 59.1, 58.6, and 58.1％ identity with TMV, PMMoV and ToMV, respectively. This is the first report of TMGMV in Korea.

• The Plant Pathology Journal
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• 제38권6호
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• pp.656-664
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• 2022

Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferumspecific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1350-1358
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• 2012

Viral pathogens, alongside other pathogens, have major effects on crustacean aquaculture. Hepatopancreatic parvovirus (HPV) is an emerging virus in the shrimp industry and has been detected in shrimp farms worldwide. The HPV genome has greater diversity than other shrimp viruses owing to its wide host range and geographical distribution. Therefore, developing diagnostic tools is essential to detect even small copy numbers from the target region of native HPV isolates. We have developed two easy to use quantitative real-time PCR kits, called Green Star and Dual Star, which contain all of the necessary components for real-time PCR, including HPV primers, using the primers obtained from the sequences of HPV isolates from Korea, and analyzed their specificity, efficiency, and reproducibility. These two kits could detect from 1 to $1{\times}10^9$ copies of cloned HPV DNA. The minimum detection limits obtained from HPV-infected shrimp were $7.74{\times}10^1$ and $9.06{\times}10^1$ copies in the Green Star and Dual Star assay kits, respectively. These kits can be used for rapid, sensitive, and efficient screening for HPV isolates from Korea before the introduction of postlarval stages into culture ponds, thereby decreasing the incidence of early development of the disease.

• 한국추진공학회지
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• 제24권4호
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• pp.79-88
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• 2020

접촉점화 추진제는 장기 저장성과 높은 점화 신뢰성을 기반으로 우주 비행체의 추진 시스템에 널리 활용되어 왔다. 기존 접촉점화 추진제는 독성이 강하고 발암성 물질로 분류되어 취급과 운용에 많은 비용이 소요된다. 이러한 기술적 단점을 극복하기 위하여, 저독성 물질에 기반한 고성능 접촉점화 추진제 조합에 대한 연구가 활발히 수행되어 왔다. 이 논문에서는 친환경 접촉점화 추진제의 개발 현황에 대한 문헌 조사를 바탕으로 과산화수소, 질산, 이온성 물질을 포함한 친환경 접촉점화 추진제의 연구 개발 동향을 소개하려 한다.

• Advances in materials Research
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• 제12권2호
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• pp.119-131
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• 2023

The development of sustainable composites materials, from recycled polymeric materials and waste from the wood industry and stone processing, allows reducing the volume of these by-products, minimizing impacts on health and the environment. Nowadays, Polypropylene (PP) is the most recycled polymer in industry, while the furniture industry has increasingly used timber felled from sustainable forest plantations as a eucalypt. The powder tailing from the ornamental stone extraction and processing industry is commonly disposed of in the environment without previous treatment. Thus, the technological option for the development of composite materials presents itself as a sustainable alternative for processing and manufacturing industries, enabling the development of new materials with special technical features. The results showed that powder granite particles may be incorporated into the polypropylene matrix associated with short eucalyptus fibres forming green hybrid composites with potential application in structural engineering, such as transport and civil construction industries.

• 디지털콘텐츠학회 논문지
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• 제12권4호
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• pp.511-518
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• 2011

최근 새롭게 제시되고 있는 중요한 패러다임 중 하나는 일상생활 및 산업 전반에서 발생되고 있는 탄소 절감을 통해 환경을 보호하여 미래를 대비하자는 것이다. 이러한 새로운 패러다임을 위해 우리나라도 녹색인증 및 녹색기술의 개발을 장려하고 녹색 성장을 중요한 정책 중의 하나로 채택하여 시행하고 있다. 또한 공공기관 및 기업들은 IT기술을 이용하여 업무 간에 발생하는 탄소절감을 하기 위해 많은 연구 및 계획들을 수립하고 실행에 옮기고 있다. 그 중에서 우리나라는 이러한 그린IT를 위해 세계 최고의 수준이라고 자부하고 있는 IT 분야의 기술력 및 모바일기기 등을 이용하여 여러 부분에서 발생되고 있는 종이 문서들 중 대체가 가능한 부분을 찾아내어 종이 문서를 사용하지 않고 전자문서로 사용하는 곳이 많다. 그러나 금융기관, 공공기관, 극장, 병원 등에서 사용되고 있는 순번발권기에서 발행되고 있는 순번 대기표는 여전히 하루에 수천에서 많게는 수 만장씩 사용되고 있다. 이렇게 발권된 순번 대기표는 거의 모든 양이 바로 버려지고 있고 재활용도 되지 않는다. 본 논문에서는 '저탄소 녹색성장'을 실천하기 위한 그린IT의 사례로서 누구나 흔히 경험하고 있는 기존 순번대기표를 대체하기 위하여 IT 환경에서 보급이 확산되고 있는 QR코드, 그리고 모바일기기를 이용하여 전자문서의 형태를 띤 'Paperless'형 순번 대기표 발행 시스템을 제안한다.

• KIEAE Journal
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• 제5권2호
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• pp.11-18
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• 2005

The Purpose of this study is development and analysis of Effluence Characteristic of the Rainfall in the IRMA Green Roof System(developed in KICT) Plus 50 program is an internal research project at KICT(Korean Institute of Construction Technology) which has it as an object ; to lengthen the building's life 50-year or more and reduce energy conception 50% than present. Green roof system is one of the most important theme in the Plus 50 program. Generally, a Green Roof System has a positive effect on the thermal conductivity in winter, the micro cooling effect on building and city by evaporation in summer, the flood-control effect by runoff-reduction or the treated rainwater-quality of green roof system and so on. However, inspection of the physical effect of green roof system does not consider in Korea. Above all, long-term monitoring and a whole observation of green roof system is needed to probate the effect. So a new experimental method could be tried in this research, which is never attempted in Korea. The measurement by a bucket with a great volume, 1L, gives a new dimension of measuring green roof effect to measure the permanent running flood from a wide roof. This offers a reasonable result on a long-term measuring of a running water. Additionally, the thermal behavior of the IRMA(Insulated Roof Membrane Assembly), known in the western europe as a reasonable solution at green roof system by economical benefits and easy construction, would be experimented.

• 한국건설관리학회논문집
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• 제15권6호
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• pp.16-25
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• 2014

최근 국가정책 아젠다인 '저탄소 녹색성장'은 국가의 중요한 비젼으로 전정부차원의 노력이 이루어지고 있다. 정부는 2020년까지 세계 7대, 2050년까지 세계 5대 녹색강국으로 진입을 목표로 하고 있다. 이러한 목표를 실현시키기 위해 효율적 온실가스 감축 및 녹색국토, 교통의 조성이 중요한 시책으로 추진되고 있다. 건설산업 부문도 예외가 아니어서 에너지 및 온실가스 배출저감을 위한 정책 및 기술개발이 활발히 이루어지고 있다. 해외 선진국의 경우 가장 광범위한 대상인 도로시설물에 대한 녹색도 측정을 위한 연구가 수행되었으며 그것을 기반으로 녹색도로인증제가 적용되고 있다. 본 논문은 선진국 제도 및 사례를 벤치마킹하고 국내 도로부문에 있어 적용성을 검증하여 녹색도로인증제도의 성공적인 국내 도입에 일조 하고자 한다.

• 한국작물학회지
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• 제49권4호
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• pp.358-362
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• 2004

In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.