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http://dx.doi.org/10.5423/PPJ.2002.18.6.323

First Report of Tobacco mild green mosaic virus Infecting Pepper in Korea  

Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration)
Kim, Jae-Hyun (National Horticultural Research Institute, Rural Development Administration)
Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
Choi, Jang-Kyung (Division of Biological Environment, Kangwon National University)
Chae, Soo-Young (National Horticultural Research Institute, Rural Development Administration)
Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration)
Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration)
Kim, Hyun-Ran (National Horticultural Research Institute, Rural Development Administration)
Choi, Yong-Mun (National Horticultural Research Institute, Rural Development Administration)
Publication Information
The Plant Pathology Journal / v.18, no.6, 2002 , pp. 323-327 More about this Journal
Abstract
A rod-shaped virus was isolated from pepper showing mild mosic during the winter growing seasons of 2001 and 2002 in Korea. Based on its biological reactions, serological relationships, reverse transcription-poly-merase chain reaction (RT-PCR) using specific primers, and nucleotide sequence analysis of coat protein (CP) gene, the isolated virus was identified as Tobacco mild green mosaic virus (TMGMV) and designated as Korean pepper isolate (TMGMV-KP). Crude sap from infected tissue was mechanically transmitted to various indicator plants, which produced characteristic symptoms of tobamovirus infection. However, no symptom was observed in Gomphorena globosa. In RT-PCR assays with specific primers toy respective detection of TMGMV, Tobacco mosaic virus (TMV), Pepper mild mottle virue (PMMoV), and Tomato mosaic virus (ToMV), a single strong band of about 500 bp in length was produced from the sample used only with TMGMV primers. The amplified DNA was cloned and the nucleotide sequence was determined. Sequence comparisons with the CP gene of other tobamoviruses indicated that TMGMV-KP shared 99.3％ identity with TMGMV Japanese isolate and only 59.1, 58.6, and 58.1％ identity with TMV, PMMoV and ToMV, respectively. This is the first report of TMGMV in Korea.
Keywords
identification; pepper; sequence; Tobacco mild green mosaic virus; Tobamovirus;
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