• ALGAE
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• 제29권2호
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• pp.153-163
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• 2014

Mesodinium rubrum is a cosmopolitan ciliate that often causes red tides. Predation by heterotrophic protists is a critical factor that affects the population dynamics of red tide species. However, there have been few studies on protistan predators feeding on M. rubrum. To investigate heterotrophic protists grazing on M. rubrum, we tested whether the heterotrophic dinoflagellates Gyrodiniellum shiwhaense, Gyrodinium dominans, Gyrodinium spirale, Luciella masanensis, Oblea rotunda, Oxyrrhis marina, Pfiesteria piscicida, Polykrikos kofoidii, Protoperidinium bipes, and Stoeckeria algicida, and the ciliate Strombidium sp. preyed on M. rubrum. G. dominans, L. masanensis, O. rotunda, P. kofoidii, and Strombidium sp. preyed on M. rubrum. However, only G. dominans had a positive growth feeding on M. rubrum. The growth and ingestion rates of G. dominans on M. rubrum increased rapidly with increasing mean prey concentration < $321ngCmL^{-1}$, but became saturated or slowly at higher concentrations. The maximum growth rate of G. dominans on M. rubrum was $0.48d^{-1}$, while the maximum ingestion rate was 0.55 ng C $predator^{-1}d^{-1}$. The grazing coefficients by G. dominans on populations of M. rubrum were up to $0.236h^{-1}$. Thus, G. dominans may sometimes have a considerable grazing impact on populations of M. rubrum.

• 한국초지조사료학회지
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• 제41권4호
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• pp.250-258
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• 2021

본 연구는 엘크 암사슴(Cervus canadensis)의 사육방식에 따른 사료섭취량, 체중변화 및 자록의 성장에 미치는 영향을 확인하고 적정 방목강도를 구명하기 위하여 수행되었다. 본 실험에 사용된 공시가축은 3~4년생 엘크 암사슴 16두(평균체중 : 236.2 ± 15.7 kg)를 이용하였으며, 방목초지는 3년 이상된 톨 페스큐 위주의 기성 혼파초지로서 초종구성은 톨 페스큐(약 50%), 오차드그라스(약 10%), 켄터기 블루그라스(약 5%)와 피, 바랭이 등으로 구성되었다. 방목초지의 수분함량은 19.51~22.61%로 방목기간 동안 유사하였으며, 조단백질 함량은 6~7월에 유의적으로 높게 나타났다. 조지방과 조회분 함량은 가을로 갈수록 점차 증가하였으며, NDF와 ADF함량은 각각 53.65~60.18%과 26.08~29.10%로 조사되었다. 실험기간동안 보충사료 섭취량은 월별로 처리구 사이에 유의적인 차이가 나타나지 않았으나, 조사료와 총 건물섭취량은 5월을 제외하고 처리구 사이에 유의적인 차이가 나타났다 (p<0.05). 특히 8월에는 GR처리구의 조사료 섭취량이 급격히 감소하였는데, 여름철 환경변화에 따른 것으로 사료된다. 반면, 9월 포유를 위해 부족한 영양소를 섭취하기 위해 사초섭취량이 급격히 증가하였다. 우리 연구에서 암사슴의 채식패턴은 수사슴과 차이를 보였으며, 이는 실험기간 자록의 이유 및 포유기간에 따른 영향을 받은 것으로 사료된다. 본 실험에서 사양방식에 따른 엘크 암사슴의 체중은 유의적 차이가 나타나지 않았으나, GR처리구에서 자록의 성장률이 빠른 것으로 나타났다. 그러나, GR처리구에서 BF처리구에 비해 낮은 이유율이 나타나 분만 전후 사사사육을 통해 이유율을 높여주는 것이 필요할 것으로 판단되며, 목지 내에서 분만 시 포유경험이 있는 산차가 높은 암사슴을 이용하고 방목강도를 높여주는 것이 이유율을 향상시키는 데 도움을 줄 수 있을 것으로 사료된다. 엘크 암사슴의 방목강도는 연평균 15두/ha로 나타났는데, 이는 다마 암사슴(Dama dama)에 비해서는 체중대비 높게 나타났으나, 엘크 수사슴에 비해서는 낮은 수치를 보여주었다. 이러한 결과는 기성초지 이용으로 인해 사초의 생산성이 감소한 결과로 사료되며, 앞으로 방목초지의 혼파방법과 기성초지의 비배관리 등을 통해 초지 활용성을 높일 수 있는 연구가 추가적으로 필요할 것으로 사료된다.

• Fisheries and Aquatic Sciences
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• 제24권12호
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• pp.415-427
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• 2021

Wetland ecosystems act as natural freshwater purification systems, but their rich biodiversity is being threatened with the introduction of the non-native freshwater snail, Pomacea canaliculata. This study was conducted to measure the herbivory effects and growth rate of P. canaliculata on common macrophytes: Ipomoea aquatica, Ipomoea batatas, Pandanus amaryllifolius and Cucurma longa. In separate experiments, the macrophyte species were served as the snails' food as individual species and simultaneously. In the individual treatment, the growth pattern and rate were based on the snails' weight (mg/snail/day; n = 9) while the individual feeding consumption (mg/snail/day) was calculated from the leftover food. In the simultaneous treatment, the herbivory effects were evaluated as the feeding preference (%) from observations every two hours, while the total feeding consumption (mg) was calculated based on the food remaining after a 12-hour experiment (3 replicates: total n = 27). The results indicated that the growth pattern was significant for snails grazing on I. aquatica but not when other macrophyte species were eaten. The individual feeding consumption was higher when using I. aquatica than P. amaryllifolius but the growth rate for snails grazing on I. aquatica and P. amaryllifolius did not differ significantly. Meanwhile, the consumption of C. longa deterred the snails' growth rate. Although the snails consumed all the macrophytes in the individual experiment, when given the species simultaneously, the feeding preference and total feeding consumption were directed significantly more toward I. aquatica than P. amaryfollius and C. longa. We conclude that P. canaliculata is a generalist feeder given a limited choice of food but tends to show a strong feeding preference after being introduced to more food choices. These findings indicate that the introduction of P. canaliculata into wetland ecosystems may increase the herbivory effects on macrophytes, making these ecosystems vulnerable to the impact of eutrophication and biodiversity reduction.

• Ocean and Polar Research
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• 제23권4호
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• pp.379-388
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• 2001

Ch1 a abundance, Ch1 a-specific productivity and phytoplankton growth rate in each size fraction (pico, $<2{\mu}m$; nano, $2-10{\mu}m$; micro, > $10{\mu}m$) in the waters around the South Shetland Islands (Ant-arctic Peninsula Area) were analysed. Although Ch1 a-specific productivity and growth rate were highest in micro-size fractions, ChI a abundance was highest in pico-size fractions. Selective removal of nano- and micro-size phytoplankton especially by krill and salp grazing, but not limitation of phytoplankton growth, seemed to be the major reason to explain this miss match between productivity and abundance of the phytoplankton community.

• 한국동물위생학회지
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• 제18권3호
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• pp.42-52
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• 1995

Anaplasmosis is most important tick-borne rickettsial diseases of domestic ruminant. with Anaptasma spp. as their respective causal agents. In order to the survey prevalence of anaplasmosis in Korean native goats, we examined the Giemsa's blood filrms from 552 grazing and 188 nongrazing-goats in Chonbuk area Namwon, Imsil and Sunchang. The conclusive diagnosis was made by observing the characteristic marginal or central bodies in the red blood cells. The infection rate of grazine and nongrazing-goats with Anaplasma spp. was 71.7% and 8.5%, respectively. In order to study about the therapeutic effects of drug for 40 grazing-oats infected with Anaptasma spp. in mountain-area, Berenil(diminazene acetate) was jntramuscularly injected (0.5㎖ /10kg B.W.). After 1 month, we don't detected the parasite in the blood films from 33 goats(82.5%), but no change of significant hematological values(PCV, ESR, WBC, RBC, MCH, MCHC, PLT, MPV, PDW and HB) was observed. These results indicated that the infection rate of Anaplasma spp. was higher on glazing than nongrazing-goats and Berenil as an antianaplasmal drug is recommended.

• Asian-Australasian Journal of Animal Sciences
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• 제15권2호
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• pp.209-212
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• 2002

The understanding of seasonal forage intake of grazing deer is essential for the development of supplementary feeding strategies in southern Australia. The alkane technique is used in other animal species for estimating feed intake of individual animals and their diet composition. To assess the potential of using alkanes as a marker for predicting feed intake of fallow deer, the daily faecal recovery of alkanes and excretion rate of dosed artificial alkanes (C32 and C36) were measured with 6 deer fed three forage based diets. The artificial alkane capsule designed for use in sheep is suitable for fallow deer. Faecal samples need to be collected over days 7-19 after dosing. The daily excretion rate was 40 mg for C32 and 37 mg for C36. The faecal recovery of natural alkanes is incomplete and the faecal concentrations of alkanes need to be adjusted for an accurate estimation of intake. The actual feed intake of 6 experimental deer over a 5 day period was accurately estimated ($R^2$=0.52) using alkanes.

• ALGAE
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• 제32권1호
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• pp.47-55
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• 2017

We explored feeding by the mixotrophic ciliate Mesodinium rubrum, heterotrophic nanoflagellates (HNFs), and small ciliates (<$30{\mu}m$ in cell length) on natural populations of heterotrophic bacteria in Masan Bay, Keum River Estuary, and in the coastal waters of the Saemankeum area, Korea when M. rubrum red tides occurred. We also measured ingestion rates of M. rubrum on cultured heterotrophic bacteria as a function of bacterial concentration in the laboratory. The ingestion rates of M. rubrum on natural populations of heterotrophic bacteria (2.3-16.8 bacteria $grazer^{-1}h^{-1}$) were comparable to or lower than those of co-occurring HNFs (10.7-41.7 bacteria $grazer^{-1}h^{-1}$), but much lower than those of co-occurring small ciliates (76.0-462.2 bacteria $grazer^{-1}h^{-1}$). However, the maximum grazing coefficient of M. rubrum ($0.245d^{-1}$) on natural populations of heterotrophic bacteria was much higher than that of small ciliates ($0.089d^{-1}}$), and slightly higher than that of HNFs ($0.204d^{-1}$). With increasing bacterial concentrations, ingestion rates of M. rubrum on cultured heterotrophic bacteria continuously increased, but became saturated at higher prey concentrations over $1-5{\times}10^6cells\;mL^{-1}$. The maximum ingestion rate of M. rubrum on cultured heterotrophic bacteria was 34.4 bacteria $grazer^{-1}h^{-1}$. Based on the present study, it is suggested that M. rubrum may be an important grazer of heterotrophic bacteria and sometimes have considerable grazing impact on natural populations of heterotrophic bacteria.

• ALGAE
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• 제35권1호
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• pp.45-59
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• 2020

To investigate the spatio-temporal distributions of the mixotrophic dinoflagellate Yihiella yeosuensis in Korean coastal waters and its grazing impact on prey populations, water samples were seasonally collected from 28 stations in the East, West, and South Seas of Korea and Jeju Island from April 2015 to October 2018. The abundances of Y. yeosuensis in the water samples were quantified using quantitative real-time polymerase chain reaction (qPCR). Simultaneously, the physical and chemical properties of water from all sampled stations were determined, and the abundances of the optimal prey species of Y. yeosuensis, the prasinophyte Pyramimonas sp. and the cryptophyte Teleaulax amphioxeia, were quantified using qPCR. Y. yeosuensis has a wide distribution, as is reflected by the detection of Y. yeosuensis cells at 23 sampling stations; however, this distribution has a strong seasonality, which is indicated by its detection at 22 stations in summer but only one station in winter. The abundance of Y. yeosuensis was significantly and positively correlated with those of Pyramimonas sp. and T. amphioxeia, as well as with water temperature. The highest abundance of Y. yeosuensis was 48.5 cells mL^-1 in Buan in July 2017, when the abundances of Pyramimonas sp. and T. amphioxeia were 917.6 and 210.4 cells mL^-1, respectively. The growth rate of Y. yeosuensis on Pyramimonas sp., calculated by interpolating the growth rates at the same abundance, was 0.49 d^-1, which is 37% of the maximum growth rate of Y. yeosuensis on Pyramimonas sp. obtained in the laboratory. Therefore, the field abundance of Pyramimonas sp. obtained in the present study can support a moderate positive growth of Y. yeosuensis. The maximum grazing coefficient for Y. yeosuensis on the co-occurring Pyramimonas sp. was 0.42 d^-1, indicating that 35% of the Pyramimonas sp. population were consumed in 1 d. Therefore, the spatio-temporal distribution of Y. yeosuensis in Korean coastal waters may be affected by those of the optimal prey species and water temperature. Moreover, Y. yeosuensis may potentially have considerable grazing impacts on populations of Pyramimonas sp.

• 환경생물
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• 제33권1호
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• pp.83-91
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• 2015

본 실험은 왕우렁이를 대상으로 대발생한 식물플랑크톤을 제어할 수 있는 가를 파악하기 위해 섭식율 (grazing rate, GR) 및 유기물 생산 (pseudofaeces production)을 조사하였다. 섭식 효율을 조사하기 위해 시간 (12시간), 개체크기, 개체밀도, 먹이농도 조건 등의 조건을 달리하여 실험하였다. 왕우렁이는 투입 후 2시간 후에 2.5 L. $gAFDW^{-1}h^{-1}$의 최고 GR를 보이다가 12시간까지 일정한 섭식 경향을 나타내었다. 유기물 생산량은 4시간 후에 15.3 mg $AFDW^{-1}$로 가장 많은 양을 나타냈다. 서로 다른 크기조건에서 각고가 1.5 cm 미만의 섭식율 변화가 크고 6시간 후에 비슷하였으며, 중간 크기(2.5 cm)가 3.0 cm 이상인 것보다 섭식율이 높았다. 밀도가 5 indiv. $L^{-1}$ 에서 엽록소 ${\alpha}$ 농도감소율이 가장 높았으며, 그 외의 밀도에서는 비슷한 변화를 나타내었다. 유기물 생산 역시 밀도가 높은 실험군에서 4시간에 최대로 증가하였다. 먹이농도가 $600{\mu}g$ $L^{-1}$일 때 0.54 L. $gAFDW^{-1}h^{-1}$의 가장높은 섭식율과 유기물 생산을 나타내었다. 본 연구에서 왕우렁이뿐만 아니라 논우렁이를 이용하여 식물플랑크톤 대발생을 제어 가능함을 본 연구를 통해 확인하였지만, 이들이 배설한 유기물의 제거 및 배설물의 분해에 다른 2차 문제에 대한 철저한 연구가 필요하다.

• 한국수정란이식학회지
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• 제32권3호
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• pp.171-176
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• 2017

본 연구는 한우 송아지 생산효율 향상을 위한 농가 번식 실태를 조사하였다. 한우 사육 규모별, 지역별로 나누어 45개 농가의 가임암소를 대상으로 조사를 실시하여 다음과 같은 결과를 얻었다. 한우의 최초 분만월령은 평균 28.7개월령이었다. 수태당 인공수정 횟수는 $1.45{\pm}0.32$회였으며, 분만 후 인공수정 일수는 119.8일이었다. 수태율은 소규모 농가가 $75.2{\pm}16.93%$로 중규모($70.6{\pm}17.46%$) 및 대규모 농가($71.4{\pm}11.03%$)보다 높은 것으로 나타났다. 번식률 상위 농가의 사육 방식을 보면, '발정관찰 보조기구를 사용하는 농가'가 '그렇지 않은 농가'보다 송아지 생산율이 10.42% 높았다. 번식소에 대해 IBR과 BVDV 예방접종을 실시한 농가는 유사산 폐사율이 4.41% 감소한 것으로 나타났다. 또한 농가여건에 따라 방목과 운동을 한 경우, 수태율과 분만율이 각각 3.47%, 18.29% 향상되는 것으로 나타났다. 본 조사 결과를 통해 발정관찰, 예방접종, 방목이 한우농가에서 송아지 생산 효율을 높이는데 중요한 지표로 나타났다. 한우 사육 농가의 번식실태 조사를 통해 농가 현장의 번식률 향상을 위한 연구 자료로 활용이 가능할 것이다.