• Korean Journal of Microbiology
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• v.55 no.1
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• pp.67-68
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• 2019

Streptococcus constellatus is Gram-stain-positive, facultative anaerobic, and non-spore forming coccus. It is a member of normal flora of human oral cavity. S. constellatus KCOM 1039 was isolated from human postoperative maxillary cyst lesion. In this report, we present the draft genome sequence of S. constellatus KCOM 1039.

• Journal of Species Research
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• v.10 no.1
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• pp.1-11
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• 2021

Evidence highlighting the importance of gut microbiota in biodiversity conservation is growing; however, gut bacteria in South Korean wildlife have not been well identified. Using a culture-dependent isolation method, we identified the gut bacteria from Korean aquatic wildlife: the gazami crab (Portunus trituberculatus), Korean striped bitterling (Acheilognathus yamatsutae), oily bitterling (Acheilognathus koreensis), leopard mandarin fish (Siniperca scherzeri), Korean dark chub (Zacco koreanus), diving beetle (Cybister lewisianus), spotted steed (Abbottina springeri), and Korean spotted sleeper (Odontobutis obscura interrupta). We identified 18 strains previously unrecorded in South Korea by comparing 16S rRNA gene sequences of isolates against the EzBioCloud and National Institute of Biological Resources(NIBR) databases. The isolated strains belong to the phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. We also assessed for phylogenetic relatedness, Gram-stain reaction, colony and cell morphology, and biochemical characteristics. Basic information and 16S rRNA gene sequences of the isolates were registered in NIBR, and NIBR accession numbers are provided.

• Journal of Species Research
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• v.11 no.1
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• pp.1-9
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• 2022

The phylum Actinobacteria includes many groups of aerobic, Gram-stain-positive, rod, or filamentous shaped bacteria. Actinobacteria are known for multicellular differentiation in some groups, and also for production of various secondary metabolites such as antibiotics. During a series of extensive surveys of indigenous prokaryotic species diversity in Korea, bacterial strains belonging to Actinobacteria were isolated from various sources of terrestrial environments. A total of 21 bacterial strains, belonging to 10 genera in 8 families, were isolated as unrecorded species in Korea. Among them, 11 were assigned to the family Streptomycetaceae, two species assigned to each of the families Microbacteriaceae, Mycobacteriaceae and Nocardioidaceae, and one species assigned to each of the families Euzebyaceae, Corynebacteriaceae, Micrococcaceae and Intrasporangiaceae. At the genus level, Streptomyces (10 species) was the most abundant, followed by Microbacterium and Mycolicibacterium(2 species each), and one species in each of the genera Corynebacterium, Euzebya, Arthrobacter, Terracoccus, Kribbella, Nocardioides and Yinghuangia. The detailed descriptions of each unrecorded species are provided.

• Journal of Species Research
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• v.12 no.spc2
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• pp.33-44
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• 2023

In 2021, a total of 27 bacterial strains were isolated from soil, tree bark, moss, wetland, sea sediment, tidal flat, seawater and seaweed within Republic of Korea. Based on the analysis of 16S rRNA gene sequence (>98.7% sequence similarity), these isolates were assigned to the class Alphaproteobacteria as unrecorded species in Korea. The 27 strains were classified into the 10 families: Maricaulaceae of the order Caulobacterales; Brucellaceae, Methylobacteriaceae, Nitrobacteraceae and Rhizobiaceae of the order Hyphomicrobiales; Micropepsaceae of the order Micropepsales; Rhodobacteraceae of the order Rhodobacterales; Azospirillaceae of the order Rhodospirillales; and Erythrobacteraceae and Sphingomonadales of the order Sphingomonadaceae. There is no official report of these 27 species in Korea. Therefore, we report 27 isolates as unrecorded species, and described isolation sources, Gram-stain reactions, physiological and biochemical properties and morphologies of these strains.

• Hip & pelvis
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• v.34 no.3
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• pp.185-190
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• 2022

Septic arthritis (SA) is a joint inflammation that develops secondary to infectious causes. SA in children is associated with a high rate of morbidity and mortality; therefore, it is regarded as an orthopedic emergency. Because SA of the hip joint usually mimics other musculoskeletal diseases, diagnosis remains challenging. Although this lesion usually shows a good outcome, treatment at an inappropriate time, neglect, or inadequate treatment could lead to poor outcomes. We report on the case of a healthy adolescent who complained of episodes of fever and chills, weight loss, pain in his left hip, and limping. After performing necessary workups, two differential diagnoses of tumor and SA were made. The results of Gram stain and culture of the synovial fluid after surgical excision showed Klebsiella pneumoniae and Enterobacter cloacae complex. To the best of our knowledge, this is the first report of SA due to co-infection with K. pneumoniae and E. cloacae in a healthy patient.

• Journal of Microbiology and Biotechnology
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• v.34 no.8
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• pp.1636-1641
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• 2024

A Gram-stain-positive, aerobic, white-coloured, rod-shaped bacteria, designated as a strain dW9^T, was isolated from soil. Strain dW9^T was catalase-positive and oxidase-negative. Strain dW9^T grew at temperature of 20-37℃ and at pH of 5.0-7.0. Phylogenetic and 16S rRNA gene analysis indicated that strain dW9^T belonged to the genus Paenibacillus with its closest relative being Paenibacillus filicis S4^T (97.4% sequence similarity). The genome size of dW9^T was 7,787,916 bp with DNA G+C G+C content of 51.3%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of dW9^T with its closest relatives were found to be <22.0% and <74.0%, respectively. The only respiratory quinone was MK-7, and the major fatty acids were antiso-C_15:0 and iso-C_16:0. Overall, the comprehensive taxonomic analysis revealed that strain dW9^T met all the fundamental criteria to be classified as a novel species within the genus Paenibacillus. Accordingly, we propose the name Paenibacillus gyeongsangnamensis sp. nov., with the type strain dW9^T (=KCTC 43431^T =NBRC 116022^T).

• Journal of Microbiology and Biotechnology
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• v.34 no.9
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• pp.1819-1825
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• 2024

An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6^T was isolated from soil in Republic of Korea. Strain WR6^T grew at temperatures of 10-37℃, at pH of 5.0-9.0, and at NaCl concentrations of 0-3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6^T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557^T (98.5%). The genome of strain WR6^T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6^T, Q-10 was sole ubiquinone; summed feature 8 (C_18:1ω7c and/or C_18:1ω6c) and C_19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6^T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6^T (=KCTC 92695^T =NBRC 116021^T).

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.8 no.2
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• pp.238-242
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• 2005

We report the case of a girl with peritoneal tuberculosis (Tb) who presented with mimicking features of ovarian cancer including massive ascites, elevation of CA-125 and ovarian masses. These ovarian masses cannot be ruled out malingnacy with image study. Gram stain, acid-fast stain, bacteriological and mycological culture of centrifuged ascites cells were negative. Tumor markers revealed an elevated CA-125 level of 112 U/mL and adenosine deaminase level were significantly high with 118 U/L. Diagnostic laparoscopy was done. The histology of the peritoneum and the ovary showed multiple granulomatous nodules with epithelioid cells and multi-nucleated giant cells. And there were no evidence of malignancy. Furthermore acid-fast bacilli (AFB) was identified in this specimen. Peritoneal Tb was difficult to diagnosis in our patient because it mimicked ovarian cancer. And this is the first case of abdominal Tb mimicking ovarian cancer in children in Korea.

• Korean Journal of Food Science and Technology
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• v.48 no.4
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• pp.361-365
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• 2016

This study was conducted to isolate and identify a microorganism that increases tricin-O-(threo-${\beta}$-guaiacylglyceryl) ether (TTGE) content in the hulls of rice (Oryza sativa L.). Bacteria from germinated rice were isolated by enrichment cultivation using yeast mold, luria bertani, potato dextrose and mannitol egg york polymyxin broths. The highest increase in TTGE content ($339.30{\mu}g/g$) was achieved by a microorganism isolated by PDA enrichment cultivation. On the basis of 16S RNA sequence homology and phylogenetic analysis, the isolated bacterium was identified to have 100% similarity with Burkholderia vietnamiensis. The isolated bacteria were short rods, negative for the Gram stain, and positive for the catalase test. The highest TTGE level was $435.86{\mu}g/g$ in 72-h fermented samples, representing a 2.5x increase compared with the control ($175.65{\mu}g/g$). In conclusion, the bacterium isolated from germinated rice extract was Burkholderia vietnamiensis, and the optimum fermentation period to maximize TTGE levels was 72 h. These findings might help in developing functional materials using rice hulls, a waste product of rice milling.

• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.1-5
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• 1978

Listeria monocytogenes infection was considered a rather rare disease and occurs mostly either in newborn babies or in young children. However, there has been increasing reports of this infection in elderly person with various underlying disease. Recently we have experienced two cases of Listeria meningitis; one in a 4-year-old male with an acute lymphoblastic leukemia, and the other in a 43-year-old female with a breast cancer. Both were on various chemotherapeutic agents for their primary diseases when the organism, L. monocytogenes was found in their celebospinal fluid(CSF). The degree of CSF pleocytosis were quite different by cases. The former case showed a marked increase, $3,350/mm^3$, and the latter slight, $410/mm^3$, Both showed a slight decrease of CSF glucose ranging 39 to 43mg/100ml. It seems that a routine CSF analysis bears a limitted value in the diagnosis or Listeria meningitis. A direct smear of CSF with Gram's stain revealed gram-positive bacilli in one case, but none in the other. Bacterial culture of CSF yielded plenty colonies in one case, but a few in the other. It seems that isolation of L. monocytogenes must not be considered very easy, and a negative direct smear does not necessarily mean a negative culture. The two isolates we obtained showed the typical cultural and biochemical characteristics of L. monocytogenes and were found to belong to serotypes 1b and 4b. It was our experience that the identification of this organism was not very much matter because of its distinct characteristics, but the most important matter was how to think of the possibility of this organism at the begining. The two isolates were both susceptible to cephalothin, chloramphenicol, erythromycin, tetracycline and gentamicin; intermediate to ampicillin, penicillin and kanamycin; and resistant to cloxacillin.