• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.132-138
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• 2023

The Gymnocalycium mihanovichii has been an important export item of Korean flower industry for a long time. Although there is a high demand for grafted cactus from overseas, its production for export is limited. In this study, the growth and marketable yield characteristics of Gymnocalycium mihanovichii 'I-Hong' were compared between soil culture and ebb-and-flow hydroponic system with single- or double-tier bench. As a result, hydroponic methods with single-tier bench resulted in higher fresh weight and glove diameter compared to other cultivation methods. In the ebb-and-flow hydroponic system, hydroponic system with double-tier bench of grafted cactus traits has a lower growth rate than other cultivations. However, the hydroponic system with double-tier bench of grafted cactus significantly increased the yield. In conclusion, the yield from hydroponic system with double-tier bench was better than soil cultivation method. Although there were some differences in color depending on the cultivation method, it was considered that there was no difference in appearance of Gymnocalycium mihanovichii 'I-Hong'. Our results suggest the cultivation methods to overcome production constraints, expand their exports, and improve the value-added characteristics of grafted cactus.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.114.1-114
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• 2003

A new isolate of rod-shaped virus was identified from grafted cactus, Gymnocalycium mihanovichii grafted onto Hylocereus trigonus, in Korea. The virus proved to be a new Tobamovirus and called previously as Tobamovirus-Ca for which we suggest the name Cactus mild mottle virus(CMMoV), because it produced systemic mild mosaic symptoms on its original host. CMMoV is distantly related to known species of the genus Tobamovirus on the basis of host range, serological and sequence analyses. Western blot analysis showed that CMMoV is serologically unrelated to Summons' Opuntia virus which is the only known species of the genus found in cactus plants. The 3'-terminal 2,910 nucleotides have been sequenced for the virus. The coat protein (CP) and movement protein (MP) genes encode 161 and 306 amino acids residues, respectively. The nucleotide and amino acid sequences of the CP were 39.6 ％ to 49.2 ％ and 26.4 ％ to 40.3 ％ identical to other tobamoviruses, respectively. The MP and 3' noncoding region shared 16.3 ％ to 23.3 ％ and 44.6 ％ to 63.4 ％ identities, respectively, with the members of the genus. Phylogenetic tree analysis of the CP gene revealed that CMMoV clusters with members of subgroup I of Tobamovirus. CMMoV particles contained genomic RNA along with two subgenomic RNAs, and this characteristics is common in the members of the subgroup II. This is the first information of sequence and comparative analysis of a Tobamovirus that infects cactus.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.67-73
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• 2009

This study was conducted to establish a strategy to improve the grafted cacti export which has been stagnated in these year. To promote its exporting, first of all, Korean cactus producer needs to save the cost of labor by using bottom nutriculture system for the greater national competitiveness. Second, it is desired to diversify the export cactus items such as a new variety of Epiphyllum truncatum, big size of perfect brand, a finished package production to satisfy foreigner's demand coping with international market change of grafted cacti. On the other hand, Korean cultivar protection and national negotiation for a reducing tariff rate or an efficient plant inspection system are also needed.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.231-237
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• 2010

The heat-inactivated (at $121^{\circ}C$ for 20 min) conidial suspension of Bipolaris cactivora (HICS) was evaluated for the control of Bipolaris stem rot of cactus caused by B. cactivora. Severe rot symptoms were developed on the cactus stem discs inoculated with B. cactivora from 5 days after inoculation. However, only small brownish spots developed on the stem discs treated with HICS 2 days prior to the pathogen inoculation. HICS also reduced symptom development on cactus stem discs inoculated with other fungal pathogens such as Alternaria alternata, Colletotrichum gloeosporioides, and Fusarium oxysporum, suggesting its disease-inhibitory efficacy may not be pathogen-specific. HICS significantly reduced severities of the stem rot disease on several cactus species including Hylocereus trigonus, Cereus peruvianus, Chamaecereus silvestrii and Gymnocalycium mianovichii, but not on Cereus tetragonus. Extensive wound periderms were formed in the stem tissues of inoculation and/or wounding sites on C. peruvianus treated with HICS alone or prior to the pathogen inoculation, but not on C. tetragonus, indicating the structural modifications may be related to the mechanism of disease suppression by HICS. HICS also reduced the disease development on the grafted cactus (H. trigonus stock and G. mianovichii scion) with the control efficacy nearly equivalent to the application of a commercial fungicide. All of these results suggest HICS can be used as an environmental-friendly agent for the control of the cactus stem disease.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.196-199
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• 2008

A new grafted cactus variety, 'Dahong', Gymnocalycium mihanovichii, is hybrid crossed between '9508021' line with red and black color, and 'Jinhong', with dark red color in 2002. It was cultivated for 6 months after grafted in vitro and planted at 100 mL test tube. After grafting in vivo in 2003, the various characteristics of this was evaluated three times until 2006. It has the flat-round shape and dark red skin, and produces the daughter globes at areoles, which are arranged well at the low part of globe. In addition, it has 8~10 ribs and the gray and slant spines in body. The ability of propagation is better than 'Jinhong' to produce approximately 13.6 daughter globes.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.342-344
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• 2012

A new grafted cactus cultivar, called 'Hwangseon', Gymnocalycium mihanovichii, is a hybrid crossed between two orange color cultivars 'Geoseong' and 'Huhwang'. Mating was performed in the greenhouse of National Institute of Horticultural & Herbal Science (NIHHS) in 2005. The harvested seeds were sown at 100 mL volumetric flasks filled with 'Kyoto' medium. Seedling had been grafted into a young rootstock, Hylocereus trigonus, and cultured in vitro in 100 mL test tube for more than six months. After re-grafting and planting in the green house in 2006, their characteristics were evaluated three times until 2009. The new cultivar has a flat round shape and bright yellow skin color. In addition, it has 7 to 9 ribs with gray and straight spines on the body and produces an average of 12.3 offsets.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.234-238
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• 2008

This experiment was conducted to investigate the effect of growth retardants such as CCC and diniconazole in grafted cactus for control shipping product of grafted cactus, which had high degree of dependence upon export. The PGRs were 50, 100, 150 $mg{\cdot}L^{-1}$ CCC and diniconazole, respectively. Treatment of diniconazole and CCC not reduced diameter on Gymnocalycium mihanovichii var. friedrichii but number of tubercle reduced significantly according to the increase of diniconazole treatment, control was 7.2 tubercles, but with 100 $mg{\cdot}L^{-1}$ diniconazole was 6.4 tubercles. Treatment of diniconazole and CCC reduced significantly diameter and tubercle number on Chamaecereus silvestrii f. variegata at higher concentration. Diameter and height reduced significantly the treatment of CCC with 3.3 and 13.4 mm of 150 $mg{\cdot}L^{-1}$, respectively. Dry weight of root, rootstock and scion decreased at higher concentrations of CCC and diniconazole in Chamaecereus silvestrii f. variegata. The most effective method of plant growth retardants for controlling of shipping time was the 150 $mg{\cdot}L^{-1}$ CCC treatment.

• Research in Plant Disease
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• v.28 no.3
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• pp.172-178
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• 2022

The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on the results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.

• The Plant Pathology Journal
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• v.28 no.2
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• pp.218-218
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• 2012

• Horticultural Science & Technology
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• v.29 no.6
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• pp.575-582
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• 2011

This experiment was conducted to study the qualitative changes of grafted cactus after harvest and to examine the decomposition characteristics of pathogenic fungi which occurs or grows during the simulated shipping period. Plant materials with four varieties of Gymnocalycium mihanovichii var. friedrichii including, 'Hukwang', 'Huhong', 'Hwangwol', 'Yeunhwa' and two varieties of Chamaecereus silvestrii f. variegate such as 'Goldcrown' and 'Yellowcrown' were used. During the simulated shipping period, the fresh-weight, bulb diameter, carbon dioxide emission rate, and decomposition rate were observed. The regeneration rate and decomposition rate were observed for the grafted cactuses that were placed in a greenhouse environment with a temperature of $28{\pm}12^{\circ}C$ and humidity of $36{\pm}15.3%$ after 40 days of simulated shipping. There were reductions in the fresh-weight and bulb diameter in every variety as time passed while the carbon dioxide emission rate showed no meaningful difference by each variety. Furthermore, the decomposition rate in the scion was higher than in the stock. According to the analysis of pathogenic fungi by decomposition characteristics, Alternaria sp., Cladosporium sp., Colletotrichum sp., Fusarium sp., Penicillium sp. in G. mihanovichii var. friedrichii were found and Alternaria sp., Bipolaris sp., Cladospoirum sp. in C. silvestrii f. variegate were identified. Therefore, to maintain and improve the quality of grafted cactus, it is necessary to analyze the factors of decomposition from the time of harvest until the point of export and develop a process technology to minimize the decomposition rate.