Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea

  • Park, Kyoung-Taek (School of Applied Biosciences, Kyungpook National University) ;
  • Hong, Soo-Min (School of Applied Biosciences, Kyungpook National University) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, San Yeong (Gumi Floriculture Research Institute, Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Ten, Leonid N. (School of Applied Biosciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.07.21
  • Accepted : 2022.09.06
  • Published : 2022.09.30
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Abstract

The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on the results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Agriculture, Food and Rural Affairs Convergence Technologies Program for Educating Creative Global Leader Program funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (no. 321001-03).

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