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http://dx.doi.org/10.5423/RPD.2022.28.3.172

First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea  

Park, Kyoung-Taek (School of Applied Biosciences, Kyungpook National University)
Hong, Soo-Min (School of Applied Biosciences, Kyungpook National University)
Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, San Yeong (Gumi Floriculture Research Institute, Gyeongsangbuk-do Agricultural Research and Extension Services)
Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University)
Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University)
Ten, Leonid N. (School of Applied Biosciences, Kyungpook National University)
Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.28, no.3, 2022 , pp. 172-178 More about this Journal
Abstract
The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on the results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.
Keywords
Graft cactus; Gymnocalycium mihanovichii; Pathogenicity; Pectobacterium brasiliense; Soft rot;
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