• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.370-374
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• 2010

A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.

• Molecules and Cells
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• v.43 no.1
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• pp.76-85
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• 2020

MARCH5 is a RING finger E3 ligase involved in mitochondrial integrity, cellular protein homeostasis, and the regulation of mitochondrial fusion and fission. To determine the function of MARCH5 during development, we assessed transcript expression in zebrafish embryos. We found that march5 transcripts were of maternal origin and evenly distributed at the 1-cell stage, except for the mid-blastula transition, with expression predominantly in the developing central nervous system at later stages of embryogenesis. Overexpression of march5 impaired convergent extension movement during gastrulation, resulting in reduced patterning along the dorsoventral axis and alterations in the ventral cell types. Overexpression and knockdown of march5 disrupted the organization of the developing telencephalon and diencephalon. Lastly, we found that the transcription of march5 was tightly regulated by the transcriptional regulators CHOP, C/EBPα, Staf, Znf143a, and Znf76. These results demonstrate the essential role of March5 in the development of zebrafish embryos.

• Journal of dental hygiene science
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• v.15 no.4
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• pp.495-502
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• 2015

In this study, it was to understand the factors influencing choice conviction of dental hygiene major graduate school of dental hygienist. This study aims to provide the basic data for developing the dental hygienic profession. The study method was complete enumeration of the master's graduate 84 students majoring in dental hygiene in the country 8 graduate school. The results of this study, the analysis of the differences between the external factors and internal factors of graduate school choice, factors of financial accessibility factor related marriage factor. Factor of the education program were more unmarried. The average monthly income was found to have recognized high financial accessibility of factors, education program. Also the carrier is low, factor of the education program it was found to be high. The result of graduate school choice conviction, the more unmarried chosen choice conviction score in high, it choice conviction score was related to monthly income. The result of correlation analysis, external reputation, future, education program if high, it was found to have conviction of graduate school. In graduate school choice conviction external factors of education program and future factor was found to influence the choice of graduate school but internal factors was not found to influence. Therefore, we must seek the support measures that can deepen the sustainable development and dental hygiene of the dental hygienist.

• Korean Journal of Health Education and Promotion
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• v.32 no.2
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• pp.39-52
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• 2015

Objectives: Residents of a single room occupancy (Jjok-bang) in Seoul are at high risk of having mental health issues. The majority of residents live in single households with past traumatic experiences including self-deprivation and social exclusion. This study was to investigate the association between mental health and socio-ecological factors at the intrapersonal and community levels. Methods: We conducted face-to-face surveys in Dongja-dong Jjok-bang area in June, 2014. Of 78 participants, 76% were male and the mean age was 60 years (SD=11.53). A multiple regression was used to analyze the association among depression, a sense of well-being, socio-ecological factors, and perceived empowerment and community solidarity. Results: Perceived empowerment (${\beta}=0.83$; 95% CI=0.40, 1.26) and community solidarity (${\beta}=0.52$; 95% CI=0.04, 1.01) were positively associated with a sense of well-being. Participants with empowerment (${\beta}=-2.55$; 95% CI=-4.86, -0.23) and those with community solidarity (${\beta}=-2.36$; 95% CI=-4.94, 0.21) were negatively associated with being depressed. Conclusion: Mental health of the residents in Jjok-bang was more influenced by empowerment and community solidarity than socio-demographic factors. It is necessary to improve public health infrastructures that can enable the residents to enhance empowerment and community solidarity utilizing socio-ecological perspectives.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.997-1004
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• 2009

Bacillus amyloliquefancies CH51 isolated from cheonggukjang, a traditional Korean fermented soy food, has strong fibrinolytic activity and produces several fibrinolytic enzymes. Among four different growth media, tryptic soy broth was the best in terms of supporting cell growth and fibrinolytic activity of this strain. A protein with fibrinolytic activity was partially purified from the culture supernatant by CM-Sephadex and Phenyl Sepharose column chromatographies. Tandem mass spectrometric analysis showed that this protein is a homolog of AprE from B. subtilis and it was accordingly named AprE51. The optimum pH and temperature for partially purified AprE51 activity were 6.0 and $45^{\circ}C$, respectively. A gene encoding AprE51, aprE51, was cloned from B. amyloliquefaciens CH51 genomic DNA. The aprE51 gene was overexpressed in heterologous B. subtilis strains deficient in fibrinolytic activity using an E. coli-Bacillus shuttle vector, pHY300PLK.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.1
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• pp.129-136
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• 2016

This study was performed to investigate whether an intra-articular injection of transient receptor potential vanilloid 1 (TRPV1) receptor agonist, resiniferatoxin (RTX) would alleviate behavioral signs of arthritic pain in a rat model of osteoarthritis (OA). We also sought to determine the effect of RTX treatment on calcitonin gene-related peptide (CGRP) expression in the spinal cord. Knee joint inflammation was induced by intra-articular injection of monosodium iodoacetate (MIA, $8mg/50{\mu}l$) and weight bearing percentage on right and left hindpaws during walking, paw withdrawal threshold to mechanical stimulation, and paw withdrawal latency to heat were measured to evaluate pain behavior. Intra-articular administration of RTX (0.03, 0.003 and 0.0003%) at 2 weeks after the induction of knee joint inflammation significantly improved reduction of weight bearing on the ipsilateral hindlimb and increased paw withdrawal sensitivity to mechanical and heat stimuli. The reduction of pain behavior persisted for 3~10 days according to each behavioral test. The MIA-induced increase in CGRP immunoreactivity in the spinal cord was decreased by RTX treatment in a dose-dependent manner. The present study demonstrated that a single intra-articular administration of RTX reduced pain behaviors for a relatively long time in an experimental model of OA and could normalize OA-associated changes in peptide expression in the spinal cord.

• Molecular Medicine Reports
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• v.20 no.3
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• pp.2476-2483
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• 2019

Atopic dermatitis (AD ) is an inflammatory skin disorder caused by immunological dysregulation and genetic factors. Whether the expression levels of cytokine and skin barrier protein were altered by S100 calcium binding protein A8 (S100A8) and S100A9 in human keratinocytic HaCaT cells was examined in the present study. Alterations of cytokine expression were examined by ELI SA following treatment with S100A8/9 and various signal protein-specific inhibitors. Activation of the mitogen activated protein kinase (MAPK) pathway and nuclear factor (NF)-κB was evaluated by using western blotting and an NF-κB activity test, respectively. The expression levels of interleukin (IL )-6, IL- 8 and monocyte chemoattractant protein-1 increased following treatment with S100A8 and S100A9, and the increase was significantly blocked by specific signaling pathway inhibitors, including toll-like receptor 4 inhibitor (TLR 4i), rottlerin, PD98059, SB203580 and BAY-11-7085. Extracellular signal-regulated kinase (ER K) and p38 MAPK pathways were activated in a time-dependent manner following treatment with S100A8 and S100A9. Phosphorylation of ER K and p38 MAPK were blocked by TLR 4i and rottlerin. S100A8 and S100A9 induced translocation of NF-κB in a time-dependent manner, and the activation of NF-κB was inhibited by TLR 4i, rottlerin, PD98059 and SB203580. In addition, S100A8 and S100A9 decreased the expression of skin barrier proteins, filaggrin and loricrin. These results may help to elucidate the pathogenic mechanisms of AD and develop clinical strategies for controlling AD.

• Environmental Engineering Research
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• v.18 no.2
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• pp.77-84
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• 2013

Recently, rainwater has come to the forefront as an alternative resource contributing to solving various environmental problems, especially those related to water. However, more or less biased perspectives on rainwater as a pollutant, mainly derived from acid rain, have still been pervasive in public as well as research communities. Considering the significance of rain harvesting in recent society, it is necessary to promote educational efforts to overcome such social prejudices and improve desired values towards rainwater. Thus, this research attempted to develop a teaching-learning program focusing on the theme of rainwater for high school students in Korea. To verify the effectiveness of the program, one group pre/post testing was conducted with 57 students participating in the courses. According to the survey, students show significant differences in both awareness and attitude towards rainwater as well as water after the courses. In conclusion, this study has an implication on developing an effective educational way to improve students' positive recognitions and attitudes regarding rainwater-related issues. Such an educational effort might contribute to sharing the worthiness of preserving rainwater with the future generation and promoting rainwater harvesting within wider public participation.

• Journal of the Korean Society of Clothing and Textiles
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• v.40 no.2
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• pp.221-239
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• 2016

This research investigates the current state of beauty related curriculums and departments of undergraduate and graduate programs in Korea. The results also compared beauty related programs at selected overseas universities. A comprehensive list of 4-year universities with beauty related undergraduate and/or graduate programs were surveyed using the KCUE University Entrance Information (http://www.adiga.kr) site provided by the Korean Council for University Education. Information on the name of the department, curriculum, and year of foundation were obtained from the websites of individual universities. There were 58 universities which have beauty related higher education programs; 43 undergraduate programs, 12 graduate programs, and 40 special graduate programs. The number of special graduate departments were 3.7 times larger than the graduate departments; in addition, 43.9% of the special graduate departments were located in the Seoul/Gyeonggi/Incheon area. The curriculums of beauty related 4-year undergraduate departments were focused on the 'Beauty care service' area (50.2%), whereas the curriculums of graduate departments were focused on the 'Cosmetic science' area (40.8%). In case of the special graduate programs, there was little difference between the 'Beauty care service' area (24.4%) and the 'Cosmetic science' area (27.7%). Beauty related programs of overseas universities were mostly focused on cosmetic science with some universities specialized in the marketing aspect.

• Journal of Embryo Transfer
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• v.25 no.1
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• pp.9-14
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• 2010

This study was conducted to find out the effects of artificial shrinkage (AS) on post-thaw development of bovine embryos. The blastocoelic cavity of blastocyst was punctured to remove its fluid contents and then incubated in the holding medium (HM) for 10 min. The punctured and non-punctured (control) blastocysts were equilibrated in vitrification solution 1 (VS1; TCM-199+20% FBS+10% EG) for 5 min and vitrification solution 2 (VS2; TCM199+20% FBS+35% EG+5% PVP+0.5 M Sucrose) for 1 min and vitrified by direct dropping into the liquid nitrogen. Vitrified blastocysts (punctured and control) were thawed and cultured in vitro (12 hr) for studying survival and hatching rates. The levels of shrinkage were measured by the volume of the blastocyst during equilibration in VS1 (at 1, 3 and 5 min of equilibration) and VS2 (at 30 and 60 sec of equilibration) that was considering the volume of non-punctured blastocyst in HM as 100%. The levels of shrinkage were higher in punctured group (62.4, 64.6, 64.3% at 1, 3 and 5 min in VS1; 50.6 and 52.7% at 30 and 60 sec in VS2) than control group (84.8, 86.6, 86.4% at 1, 3 and 5 min in VS1; 72.1 and 68.8% at 30 and 60 sec in VS2), but within each group the levels of shrinkage were similar. The survival (90.9%) and hatching (50.0%) rates of vitrified blastocysts at 12 hr post-thaw were higher in punctured group than that in control group (76.9% and 0.0% respectively). We confirmed that vitrification solutions (VS1 and VS2) have no toxic effect on the survival of blastocysts because the survival rates of blastocysts exposed to VS1 and VS2 for 24 hr were similar between punctured and control groups (94.3 vs. 96.0%; p>0.05). In conclusion, the preliminary data show that AS of blastocyst may improve survival and hatching rate after thawing.