• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.58-58
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.46-46
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• 2005

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1824-1828
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• 2016

AcrR, the toxic-compounds-response regulator, regulates motility in microorganisms, presumably to escape from toxic environments. In this study, the genome-wide target genes of AcrR were investigated in a ΔacrR mutant strain by microarray analysis. In the absence of AcrR, the transcription of most flagella/motility genes was highly increased. In addition, flagella formation was increased in this mutant strain. Motility assays revealed that AcrR modulates swimming motility, but not swarming.

• Journal of Biomedical Engineering Research
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• v.39 no.1
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• pp.10-15
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• 2018

In this paper, we present a wearable measurement system for monitoring rounded shoulders. The system contains a shoulder correction band and a stretch sensor that can correct and measure shoulder posture, respectively. The capacitance of the stretch sensor changes linearly according to changes in the shoulders. To verify measurement, a motion analysis system was used as the reference to compare the change in the rounded angles of the shoulders and the change in the stretch sensor's capacitance. The results indicated that there is a high correlation between the two changes and the system can be used as a monitoring device for rounded shoulders.

• Child Health Nursing Research
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• v.14 no.3
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• pp.269-276
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• 2008

Purpose: In this study the effects of Self-Development program on self-esteem and communication in middle school students were examined. Method: Forty-three middle school students in D City participated in a survey, which was carried out from October to November, 2007. To test the effects of the Self-Development Program, the students were divided into two groups, an experimental group (23) and a control group (20). The research design used in this study was a nonequivalent control group pre-posttest quasi-experimental research design. The research tools included a self-esteem scale and a communication scale. Chi-square test, paired t-test, and t-test were used to analyze the data with the SPSS/WIN 12.0 program. Result: Significant differences were found in self-esteem scores between the experimental group and the control group. But there were no significant differences between the two groups on communication scores. Conclusion: The results suggest that the Self-Development program is a useful nursing intervention for improving the self-esteem of middle school students.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.6
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• pp.565-574
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• 2021

Astrocytes are activated in response to brain damage. Here, we found that expression of Kir4.1, a major potassium channel in astrocytes, is increased in activated astrocytes in the injured brain together with upregulation of the neural stem cell markers, Sox2 and Nestin. Expression of Kir4.1 was also increased together with that of Nestin and Sox2 in neurospheres formed from dissociated P7 mouse brains. Using the Kir4.1 blocker BaCl₂ to determine whether Kir4.1 is involved in acquisition of stemness, we found that inhibition of Kir4.1 activity caused a concentration-dependent increase in sphere size and Sox2 levels, but had little effect on Nestin levels. Moreover, induction of differentiation of cultured neural stem cells by withdrawing epidermal growth factor and fibroblast growth factor from the culture medium caused a sharp initial increase in Kir4.1 expression followed by a decrease, whereas Sox2 and Nestin levels continuously decreased. Inhibition of Kir4.1 had no effect on expression levels of Sox2 or Nestin, or the astrocyte and neuron markers glial fibrillary acidic protein and β-tubulin III, respectively. Taken together, these results indicate that Kir4.1 may control gain of stemness but not differentiation of stem cells.

• Journal of Korean Society of Rural Planning
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• v.12 no.4 s.33
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• pp.115-123
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• 2006

The direct payment program for rural landscape conservation in practice since 2005. Recently it is the policy or plan of direct payment for rural landscape conservation attracts great interests as they may help rural people who are very disadvantaged. This paper presents the status of the direct payment program for landscape conservation in rural area and proposed improvements to the program as following; 1) to diversify the plant species, 2) to include other rural elements than plants, 3) to enlarge the areas of crop plantation, 4) to simplify and clarify application process. We sampled two pilot programs, one of spring flower crop and the other of autumn flower crop, and questionnaire surveyed visitors, local government officers and farmers in order to find problems and suggest improvements.

• BMB Reports
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• v.55 no.6
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• pp.281-286
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• 2022

Hepatocellular carcinoma is a major health burden, and though various treatments through much research are available, difficulties in early diagnosis and drug resistance to chemotherapy-based treatments render several ineffective. Cancer stem cell model has been used to explain formation of heterogeneous cell population within tumor mass, which is one of the underlying causes of high recurrence rate and acquired chemoresistance, highlighting the importance of CSC identification and understanding the molecular mechanisms of CSC drivers. Extracellular CSC-markers such as CD133, CD90 and EpCAM have been used successfully in CSC isolation, but studies have indicated that increasingly complex combinations are required for accurate identification. Pseudogene-derived long non-coding RNAs are useful candidates as intracellular CSC markers - factors that regulate pluripotency and self-renewal - given their cancer-specific expression and versatile regulation across several levels. Here, we present the use of microarray data to identify stemness-associated factors in liver cancer, and selection of sole pseudogene-derived lncRNA ZNF204P for experimental validation. ZNF204P knockdown impairs cell proliferation and migration/invasion. As the cytosolic ZNF204P shares miRNA binding sites with OCT4 and SOX2, well-known drivers of pluripotency and self-renewal, we propose that ZNF204P promotes tumorigenesis through the miRNA-145-5p/OCT4, SOX2 axis.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.623-630
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• 2008

A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1629-1637
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• 2007

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.