• Journal of Microbiology and Biotechnology
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• v.32 no.2
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• pp.160-169
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• 2022

In the present study we evaluated the efficacy of a bioformulation of Streptomyces cameroonensis for control of black pod disease in cocoa and enhancement of seedling growth. The formulation developed using talc powder and cassava starch as carriers showed high shelf-life of 1.07 × 10⁶ CFU/g after six months storage at 4℃. The formulation was tested for inhibition of spore germination in Phytophthora megakarya and showed 100% inhibition at 10% (w/v) of formulation. To determine the efficacy of the formulation, we performed an in planta assay in the greenhouse on two hybrids of cocoa seedlings, the tolerant SNK413 × (♂) T79/467 and the susceptible UPA 134× (♂) SCA 12. Detached leaf assay showed a significant reduction in the disease severity index of about 67% for the tolerant hybrid and 55% for the susceptible hybrid compared to non-treated plants. A significant enhancement in stem length, leaf surface area and root weight was observed. Analysis of biochemical markers of defense showed a significant increase in total polyphenol, flavonoid, and total protein contents. There was also significant upregulation of PR-proteins such as chitinases, peroxidases and β-1, 3-glucanases following treatment of both tolerant and susceptible hybrids, though with a higher level of synthesis in the tolerant hybrids. A significant increase was also observed in polyphenol oxidase activities in plants treated with the formulation. This work demonstrated the stability and effectiveness of the S. cameroonensis powder formulation in suppressing black pod disease in cocoa and subsequently enhancing the growth of seedlings.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.543-551
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• 2021

Nocardiopsis species produce bioactive compounds, such as antimicrobial and anti-cancer agents and toxins. However, no reports have described their anti-inflammatory and anti-fibrotic effects during nasal polyp (NP) formation. In this study, we investigated whether marine-derived bacterial Nocardiopsis sp. 13G027 exerts anti-inflammatory and anti-fibrotic effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and transforming growth factor (TGF)-β1-induced NP-derived fibroblasts (NPDFs). Nitric oxide (NO) and prostaglandin E₂ (PGE₂) levels were analyzed. Extract from Nocardiopsis sp. 13G027 significantly inhibited the upregulation of NO and PGE₂ in LPS-activated RAW 264.7 macrophages. The expression of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt/PKB) in LPS-induced RAW 264.7 macrophages was evaluated; smooth muscle alpha-actin (α-SMA), collagen type I (Col-1), and fibronectin also phosphorylated small mothers against decapentaplegic (SMAD) 2 and 3 in TGF-β1-stimulated NPDFs. The Nocardiopsis sp. 13G027 extract suppressed the phosphorylation of MAPKs and Akt and the DNA-binding activity of activator protein 1 (AP-1). The expression of pro-fibrotic components such as α-SMA, Col-1, fibronectin, and SMAD2/3 was inhibited in TGF-β1-exposed NPDFs. These findings suggest that Nocardiopsis sp. 13G027 has the potential to treat inflammatory disorders, such as NP formation.

• Journal of Plant Biotechnology
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• v.49 no.2
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• pp.139-144
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• 2022

The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

• Journal of architectural history
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• v.32 no.1
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• pp.61-72
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• 2023

Korean architecture classifies Banja (the decorated flat of the ceiling visible from the inside) of Royal Palaces into two types: Woomul(water-well, 井) banja, which inserts rectangular wooden board into lattice frame, and paper banja, which applies paper to the flat ceiling. Such classification was established in the 19th century. Before that, Banja was classified according to what was inserted into the lattice frame, either wooden or paper board. At first, the banja that used paper board was widely installed regardless of the purpose or nobility of the building. However, since the 17th century, the use of paper board banja became mostly restricted to Ondol (Korean floor heating system) rooms which are characterized by private usage and the importance of heating, and it was considered inferior to wooden board banja in terms of rank or grace. The contemporary paper banja was mainly installed in low-rank ondol rooms until the late 19th century to early 20th century, when roll-type wallpaper was introduced from the West and the paper banja came to decorate the King's and Queen's bedrooms. The traditional paper board banja benefits heat reservation, reduces the weight of the ceiling, and allows the adjustment of the lattice frame size. Furthermore, it can feature unique artistry if covered with blue, white, or red Neung-hwa-ji (traditional flower pattered paper).

• Journal of Forest and Environmental Science
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• v.39 no.1
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• pp.55-64
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• 2023

Properties of a lesser-used wood species were investigated to determine its potential for structural utilization. Trees of Delonix regia were felled and sampled at the base, middle and top and then sectioned to inner wood, middle wood, and outer wood for variation across the axial and radial directions. Hence, selected physical and mechanical properties as well as natural durability of D. regia along the radial and axial directions were examined. Obtained data were analyzed using analysis of variance (ANOVA) at α_0.05. There was no significant difference in the Moisture content (MC) of the wood but specific gravity (SG) decreased from base to top ranging from 0.35-0.44. Water absorption, volumetric swelling, and volumetric shrinkage range from 46.18-51.86%, 2.57-4.02%, and 2.26-3.96% respectively along the axial plane. The weight loss for graveyard exposure and accelerated laboratory decay test ranged from 25.14-48.00% and 32.02-44.45% respectively. Modulus of Rupture and Modulus of Elasticity values range from 29.42-72.68 Nmm² and 3,834.54-8,830.37 Nmm² respectively. The SG values has confirmed the species as a medium density wood and values of other properties tested showed that the wood is dimensional stable and moderately resistance to fungi and termite. Hence, it could be used for light construction purposes such as furniture and other interior woodwork.

• Journal of Marine Life Science
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• v.8 no.1
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• pp.56-67
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• 2023

Marine macroalgae are important in coastal ecosystems and interact with marine microorganisms. In this study, we isolated fungi from seven types of marine macroalgae including Cladophora sp., Gloiopeltis furcate, Gracilariopsis chorda, Hydroclathrus clathratus, Prionitis crispata, Sargassum micracanthum, and Ulva lactuca collected in Korea. Morphological and phylogenetic analyses identified the isolates as four Aspergillus spp. (A. fumigatus, A. sydowii, A. tamarii, and A. terreus), three Penicillium spp. (P. crustosum, P. jejuense, and P. rubens), and Cladosporium tenuissimum. Among them, A. fumigatus TOP-U2, A. tamarii SH-Sw5, and A. terreus GJ-Gf2 strains showed the activities of all enzymes examined (amylase, chitinase, lipase, and protease). Based on the enzymatic index (EI) values in solid media, A. terreus GJ-Gf2 and C. tenuissimum UL-Pr1 exhibited the highest amylase and lipase activities, respectively. Chitinolytic activity was only observed in A. terreus GJ-Gf2, A. tamarii SH-Sw5, and A. fumigatus TOP-U2. Penicillium crustosum UL-Cl2 and C. tenuissimum UL-Pr1 showed the highest protease activities. To the best of our knowledge, this is the first report of lipolytic and proteolytic activities in a marine-derived C. tenuissimum strain. Overall, the fungal strains isolated from the marine macroalgae in this study actively produced industrially important enzymes.

• Journal of The Korean Society of Agricultural Engineers
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• v.65 no.3
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• pp.57-67
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• 2023

A flash drought is a rapid-onset drought that develops over a short period of time as weather and environmental factors change rapidly, unlike general droughts, due to meteorological abnormalities. Abnormally high evapotranspiration rates and rapid declines in soil moisture increase vegetation stress. In addition, crop yields may decrease due to flash droughts during crop growth and may damage agricultural and economic ecosystems. In this study, Flash Drought Intensity Index (FDII) based on soil moisture data from Gravity Recovery Climate Experiment (GRACE) was used to analyze flash drought. FDII, which is calculated using soil moisture percentile, is expressed by multiplying two factors: the rate of intensification and the drought severity. FDII was developed for domestic flash drought events from 2014 to 2018. The flash drought that occurred in 2018, Chungcheongbuk-do showed the highest FDII. FDII was higher in heat wave flash drought than in precipitation deficit flash drought. The results of this study show that FDII is reliable flash drought analysis tool and can be applied to quantitatively analyze the characteristics of flash drought in South Korea.

• Journal of Preventive Medicine and Public Health
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• v.56 no.3
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• pp.255-263
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• 2023

Objectives: The study investigated the association between indoor air pollution (IAP) and risk factors for acute respiratory infection (ARI) symptoms in children under 5 years of age. Methods: A cross-sectional study was conducted using data derived from Indonesia Demographic and Health Survey in 2017. Binary logistic regression modeling was employed to examine each predictor variable associated with ARI among children under 5 years of age in Indonesia. Results: The study included a total of 4936 households with children. Among children under 5 years old, 7.2% reported ARI symptoms. The presence of ARI symptoms was significantly associated with the type of residence, wealth index, and father's smoking frequency, which were considered the sample's socio-demographic characteristics. In the final model, living in rural areas, having a high wealth index, the father's smoking frequency, and a low education level were all linked to ARI symptoms. Conclusions: The results revealed that households in rural areas had a substantially higher level of reported ARI symptoms among children under 5 years old. Furthermore, the father's smoking frequency and low education level were associated with ARI symptoms.

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.101-111
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• 2015

The purpose of this study is to identify any differences in academic records of students after they got into the Korea National College of Agriculture and Fisheries through the entrance exam. The target group was the students of 1,035 who enrolled from the year of 2012 to 2014 by the exams respectively, and this study compared those first year's records. As a result, this study found that their academic achievements have no significant differences statistically. With regard to the result, we could recognize that the average achievements of students are arithmetically high, who were accepted by admission officer's interview(talented students of agriculture and fisheries) and regular admission procedure to which the scholastic test result is applied. Accordingly, it is more desirable that we should rather focus on measures to improve the admission procedure that could contribute to academic achievements of students who are mostly accepted through the nonscheduled admission. It is pointless to focus on discussion that the achievement of students selected by a particular procedure is low. That kind of criticism proved groundless. Secondly, the study of the correlation between their admission records and after admission records of the freshmen in 2012 shows that a significant difference does not exist whereas those of in 2013 and 2014 has a slightly different statistically, though a coefficient of correlation was a mere 0.17. It account for this result that KNCAF has increased the applied-rate of highschool transcript from 33.3% to 50.0.% in admission procedure from the year of 2013. However, lowering the applied-rate of farming and fishing background is not everything as we consider the graduates' easy settling into rural area. It is a well-known fact that after graduation, the majority of students who have applied for a grace period of the delay in performing the obligation of farming and fishing, and of students who have paid the school expenses back came from ones with no sufficient foothold. This means that we should formulate a supporting policy for the students to settle into rural area smoothly after graduation if we keep the regulation of lowing the applied-rate of farming and fishing.

• Journal of Daesoon Thought and the Religions of East Asia
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• v.3 no.2
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• pp.93-119
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• 2024

The introductionof Won Buddhism to the United States has reached its fifty-year mark. Brought to the West by Korean kyomus (Won Buddhist clergy), these initial Won Buddhist clergy set a foundation for future ordained devotees to reside in America and further the religion's mission. Innovation has always played an important role in the formation and growth of Won Buddhism. The founder, Sotaesan, declared the necessity to reform traditional Buddhism to make it accessible to the laity and espoused values such as inclusiveness, equality, public work, and practicality. Over the past few decades, these innovations have helped Won Buddhism in America to shift from a strictly ethnic-related context to an emphasis on its universal nature. However, as the religion continues achieving a foothold in Western soil, critical questions arise such as how can Won Buddhism honor its Korean origins while becoming increasingly international? What are the detriments to decontextualizing and de-emphasizing elements thought to be "too Korean" or "too traditional," or thought to be irrelevant in the West? When Buddhism spreads to a new country, it not only influences the culture it enters but is also shaped by the adopting culture. In American history, this has often meant the erasure of Asian cultures that were home to Buddhism for millennia and from which the dharma is inextricable. I argue in this article that if Won Buddhism is to thrive in the United States conscious consideration will have to be given to the indispensable aspects of its Korean roots and tradition while connecting with the current circumstance in fresh, relevant, and effective ways that include the multi-cultural and ethnic makeup of the US. This entails understanding American history and Asian Buddhism's history in America, as well as cultivating a competency or fluency in the cultures that allowed Won Buddhism to survive for decades.