• Title/Summary/Keyword: Gossypin

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Gossypin Protects Primary Cultured Rat Cortical Cells from Oxidative Stress- and $\beta$-Amyloid-Induced Toxicity

  • Yoon, Injae;Lee, Kwang-Heun;Choi, Jungsook
    • Archives of Pharmacal Research
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    • v.27 no.4
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    • pp.454-459
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    • 2004
  • The present study investigated the effects of gossypin, 3,3',4',5,7,8-hexahydroxyflavone 8-glucoside, on the toxicity induced by oxidative stress or $\beta$-amyloid ($A_{\beta}$) in primary cultured rat cortical cells. The antioxidant properties of gossypin were also evaluated by cell-free assays. Gossypin was found to inhibit the oxidative neuronal damage induced by xanthinelxanthine oxidase or by a glutathione depleting agent, D,L-buthionine (S,R)-sulfoximine. In addition, gossypin significantly attenuated the neurotoxicity induced by $A_{{\beta}(25-35)}$. Furthermore, gossypin dramatically inhibited lipid peroxidation initiated by $Fe^{2+}$ and ascorbic acid in rat brain homogenates. It also exhibited potent radical scavenging activity generated from 1 ,1-diphenyl-2-picrylhydrazyl. These results indicate that gossypin exerts neuroprotective effects in the cultured cortical cells by inhibiting oxidative stress- and $A_{\beta}$-induced toxicity, and that the antioxidant properties of gossypin may contribute to its neuroprotective actions.

Cytotoxic and Antibacterial Activities of Gossypin

  • Chamundeeswari, D.;Sukumar, Ethirajan;Amar, K.;Reddy, N. Brahmananda;Kumar, I. Anil;Chowdry, B.V.;Samatha, K.
    • Natural Product Sciences
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    • v.13 no.4
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    • pp.300-303
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    • 2007
  • Gossypin, a naturally occurring polyhydroxy flavonoid, when subjected to in vitro cytotoxic screening against vero cell lines exhibited 68.75% inhibition at a concentration of $1000{\mu}g$. When tested against five bacteria and five fungi, the flavone derivative showed a moderate activity against Staphylococcus aureus and Escherichia coli, mild inhibition against Pseudomonas aerugenosa and Salmonella typhi and no activity against any of the tested fungi, in the concentrations studied.

Neuroprotective effects of gossypin on beta-amyloid- and oxidative stress-induced toxicity in primary cultured rat cortical cells

  • Yoon, In-Jae;Lee, Kwang-Heun;Cho, Jung-Sook
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.142.1-142.1
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    • 2003
  • Excessive accumulation of beta-amyloid (A$\beta$) peptides is one of the leading hypotheses to explain neurodegenerative processes in Alzheimer's disease (AD). It has been suggested that $A\beta$ toxicity is associated with increases in reactive oxygen species. whose overproduction may in turn initiate neurotoxic events. (omitted)

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Antiviral Activity of Some Flavonoids on Herpes Simplex Viruses (수종 Flavonoid의 항허피스바이러스효과)

  • Lee, Ji-Hyun;Kim, Young-So;Lee, Chong-Kil;Lee, Hyuk-Koo;Han, Seong-Sun
    • Korean Journal of Pharmacognosy
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    • v.30 no.1
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    • pp.34-39
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    • 1999
  • To search for less toxic antiherpetic agents, the inhibitory effects of twelve kinds of flavonoids including chrysin, quercetin, quercitrin, rutin, fisetin, gossypin, kaempferol, morin, naringenin, naringin, hesperetin and hesperidin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by plaque reduction assay in vitro. Some flavonoids tested in this study showed potent antiherpetic activity, reducing intracellular replication of herpes simplex viruses when Vero cell monolayers were infected and subsequently cultured in medium containing flavonoids. Naringenin showed the most potent antiviral activity against HSV-1 with selectivity index (SI) of 19.1 and hesperetin showed the most potent antiviral activity against HSV-2 with SI of 9.8. These results suggest that some flavonoids may be a potential therapeutic agent for the treatment and prevention of herpes simplex virus infections.

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UHPLC/TOFHRMS analysis and anti-inflammatory effect of leaf extracts from Zizyphus jujuba in LPS-stimulated RAW264.7 cells

  • Hyun Ji Eo;Sun-Young Lee;Gwang Hun Park
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.27-33
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    • 2023
  • Zizyphus jujube is a plant in the buckthorn family (Rhamnaceae) that has been the subject of research into antibacterial, antifungal and anti-inflammatory properties of its fruit and seed. However, few studies have investigated its leaves. In this study, the anti-inflammatory activity of ZJL (an extract of Z. jujube leaf) was evaluated to verify its potential as an anti-inflammatory agent and SARS-CoV-2 medicine, using nitric oxide (NO) assay, RT-PCR, SDSPAGE, Western blotting, and UHPLC/TOFHRMS analysis. We found that ZJL suppresed pro-inflammatory mediators such as NO, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. ZJL acted by inhibiting NF-KB and MAPK signaling pathway activity. We also confirmed that ZJL contains a phenol compound and flavonoids with anti-inflammatory activity such as trehalose, maleate, epigallocatechin, hyperoside, catechin, 3-O-coumaroylquinic acid, rhoifolin, gossypin, kaempferol 3-neohesperidoside, rutin, myricitrin, guaiaverin, quercitrin, quercetin, ursolic acid, and pheophorbide a. These findings suggest that ZJL may have great potential for the development of anti-inflammatory drugs and vaccines via inhibition of NF-κB and MAPK signaling in LPS-induced RAW264.7 cells.