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Gossypin Protects Primary Cultured Rat Cortical Cells from Oxidative Stress- and $\beta$-Amyloid-Induced Toxicity  

Yoon, Injae (Department of Psychiatry and Department of Pharmacology, College of Medicine, Dongguk University)
Lee, Kwang-Heun (Department of Psychiatry and Department of Pharmacology, College of Medicine, Dongguk University)
Choi, Jungsook (Department of Psychiatry and Department of Pharmacology, College of Medicine, Dongguk University)
Publication Information
Archives of Pharmacal Research / v.27, no.4, 2004 , pp. 454-459 More about this Journal
Abstract
The present study investigated the effects of gossypin, 3,3',4',5,7,8-hexahydroxyflavone 8-glucoside, on the toxicity induced by oxidative stress or $\beta$-amyloid ($A_{\beta}$) in primary cultured rat cortical cells. The antioxidant properties of gossypin were also evaluated by cell-free assays. Gossypin was found to inhibit the oxidative neuronal damage induced by xanthinelxanthine oxidase or by a glutathione depleting agent, D,L-buthionine (S,R)-sulfoximine. In addition, gossypin significantly attenuated the neurotoxicity induced by $A_{{\beta}(25-35)}$. Furthermore, gossypin dramatically inhibited lipid peroxidation initiated by $Fe^{2+}$ and ascorbic acid in rat brain homogenates. It also exhibited potent radical scavenging activity generated from 1 ,1-diphenyl-2-picrylhydrazyl. These results indicate that gossypin exerts neuroprotective effects in the cultured cortical cells by inhibiting oxidative stress- and $A_{\beta}$-induced toxicity, and that the antioxidant properties of gossypin may contribute to its neuroprotective actions.
Keywords
eossypin; Neuroprotection; Oxidative damage; Cortical neurons; Beta-amyloid; Antioxidant;
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Times Cited By Web Of Science : 15  (Related Records In Web of Science)
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1 Ishige, K., Schubert, D., and Sagara, Y., Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Rad. Biol. Med., 30,433-446 (2001)   DOI   ScienceOn
2 Johnson, M. K. and Loo, G., Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat. Res., 459, 211-218 (2000)   DOI   ScienceOn
3 Jung, Y.-S., Kang, T-S., Yoon, J.-H., Joe, B.-Y., Lim, H.-J., Seong, C.-M., Park, W.K., Kong, J. Y., Cho, J., and Park, N. S., Synthesis and evaluation of 4-hydroxyphenylacetic acid amides and 4-hydroxycinnamamides as antioxidants. Bioorg. Med. Chem. Lett., 12,2599-2602 (2002)   DOI   ScienceOn
4 Reiter, R. J., Oxidative processes and antioxidative defense mechanism in the aging brain. FASEB J., 9, 526-533 (1995)   DOI
5 Simonian, N. A. and Coyle, J. T., Oxidative stress in neurodegenerative diseases. Ann. Rev. Pharmacol. Toxicol., 36, 83-106 (1996)   DOI   ScienceOn
6 Viswanathan, S., Sambantham, P. T., Reddy,K., and Kameswaran, L., Gossypin-induced analgesia in mice. Eur. J. Pharmacol., 98,289-291 (1984)   DOI   ScienceOn
7 Cho, J., Kim, Y. H., Kong, J.-Y., Yang, C.-H., and Park, C.-G., Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sci., 71,591-599 (2002)   DOI   ScienceOn
8 Formica, J. V. and Regelson, W., Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol., 33,1061-1080 (1995)   DOI   ScienceOn
9 Griffith, O. W. and Meister, A., Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine). J Biol. Chem., 254, 7558-7560 (1979)   PUBMED
10 Behl, C. and Moosmann, B., Causes and consequences of oxidative stress in Alzheimer's disease. Free Rad. Biol. Med., 33,182-191 (2002)   DOI   ScienceOn
11 Hensley, K., Carney, J. M., Mattson, M. P., Aksenova, M., Harris, M., Wu, J. F., Floyd, R. A., and Butterfield, D. A., A model for beta-amyloid aggregation and neurotoxicity based on free radical generation by the peptide: relevance to Alzheimer disease. Proc. Natl. Acad. Sci. USA, 91, 3270-3274 (1994)   DOI   ScienceOn
12 Dyrks, T., Dyrks, E., Harmann, R., Masters, C., and Beyreuther, K., Amyloidogenecity of $\beta$A4 and $\beta$A4-bearing amyloid protein precursor fragments by metal-catalyzed oxidation. J Biol. Chem., 267,18210-18217 (1992)   PUBMED
13 Harkany, T., Hortobaqyi, T., Sasvari, M., K6nya, C., Penke, B., Luiten, P. G. M., and Nyakas, C., Neuroprotective approaches in experimental models of $\beta$-amyloid neurotoxicity: relevance to Alzheimer's disease. Prog. Neuropsychopharmacol. Biol. Psychiatry, 23, 963-1008 (1999)   DOI   ScienceOn
14 Dok-Go, H., Lee, K. H., Kim, H. J., Lee, E. H., Lee, J., Song, Y. S., Lee, Y. H., Jin, C., Lee, Y. S., and Cho, J., Neuroprotective effects of antioxidativeflavonoids,quercetin, (+)-dihydroquercetin and quercetin 3-methyl ether, isolated from Opuntia ficusindica var. saboten. Brain Res., 965, 130-136 (2003)   DOI   PUBMED   ScienceOn
15 Harris, M. E., Hensley, K., Butterfield, D. A., Leedle, A., and Caney, J. M., Direct evidence of oxidative injury produced by the Alzheimer's beta-amyloid peptide (1-40) in cultured hippocampal neurons. Exp. Neurol., 131,193-202 (1995)   DOI   ScienceOn
16 Ferrandiz, M. L. and Alcaraz, M. J., Anti-inflammatory activity and inhibition of arachidonic acid metabolism by flavonoids. Agents Actions, 32, 283-288 (1991)
17 Li, Y., Maher, P., and Schubert, D., A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion. Neuron, 19,453-463 (1997)   DOI   ScienceOn
18 Cho, J. and Lee, H.-K., Wogonin inhibits excitotoxic and oxidative neuronal damage in primary cultured rat cortical . cells. Eur. J. Pharmacol., 485,105-110 (2004)   DOI   PUBMED   ScienceOn
19 Muller, W. E., Romero, F. J., Perovic, S., Pergande, G., and Pialoglou, P., Protection of flupirtine on beta-amyloid-induced apoptosis in neuronal cells in vitro: prevention of amyloidinduced glutathione depletion. J. Neurochem., 68, 2371-2377 (1997)   DOI   PUBMED   ScienceOn
20 Cho, J., Joo, N. E., Kong, J.-Y., Jeong, D.-Y., Lee, K. D., and Kang, B.-S., Inhibition of excitotoxic neuronal death by methanol extract of Acori graminei rhizoma in cultured rat cortical neurons. J. Ethnopharmacol., 73, 31-37(2000)   DOI   ScienceOn
21 Cho, J., Kong, J.-Y., Jeong, D.-Y., Lee, K. D., Lee, D. U., and Kang, B.-S., NMDA receptor-mediated neuroprotection by essential oils from rhizomes of Acorus gramineus. Life Sci., 68,1567-1573 (2001)   DOI   ScienceOn
22 Behl, C., Davis, J. B., Lesley, R., and Schubert, D., Hydrogen peroxide mediates amyloid-beta protein toxicity. Cell, 77, 817-827(1994)   DOI   PUBMED   ScienceOn
23 Halliwell, B., Reactive oxygen species and the central nervous system. J. Neurochem., 59, 1609-1623 (1992)   DOI   PUBMED   ScienceOn
24 Viswanathan, S., Thirugnanasambantham, P., Ramaswamy, S., and Bapna, J. S., A study on the role of cholinergic and gamma amino butyric acid systems in the anti-nociceptive effect of gossypin. Clin. Exp. Pharmacal. Physiol., 20, 193-196 (1993)   DOI
25 Zhang, L., Zhao, B., Yew, D. T., Kusiak, J. W., and Roth, G. S., Processing of Alzheimer's amyloid precursor protein during $H_{2}O_{2}$-induced apoptosis in human neuronal cells. Biachem. Biophys. Res. Commun., 235, 845-848 (1997)   DOI   ScienceOn
26 Smith, M. A., Perry, G., Richey, P. L., Sayre, L. M., Anderson, V. E., Beal, M. F., and Kowall, N., Oxidative damage in Alzheimer's disease. Nature, 382, 120-121 (1996)   PUBMED
27 Bains, J. S. and Shaw, C. A., Neurodegenerative disorders in humans: the role of glutathione in oxidative stress-mediated neuronal death.Brain Res. Rev., 25, 335-358 (1997)   DOI   ScienceOn