• Journal of Ginseng Research
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• 제27권2호
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• pp.86-91
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• 2003

KT&G 중앙연구원 원료연구소에서 육성한 신품종 고풍에 대해서 육성경과, 생산력 검정 및 지역 적응시험을 수행하였다. 1968년에 선발하여 20년 이상 육성된 고풍은 줄기, 잎자루, 꽃대 및 잎맥부위까지도 진한 자색을 띤다. 열매송이 모양은 역삼각형이며 진한 붉은 색으로 재래혼계종인 자경종이나 기개발된 천풍 및 연풍과 지상부 표현형에서의 차이를 나타내었 다. 1981-1984년에 실시한 생산력 검정시험이나 1985-1990년에 실시한 지역적응력시험에서 대비구인 재래혼계종인 자경종과의 지상부 생육 제반 특성에서는 차이가 없었으나 유전적 특성의 발현 재현성이 있었다. 지하부 형질에서는 고풍의 생존율이 대비구인 자경종보다 2.6% 높았고, 고풍의 칸당 수량은 2.3 kg으로 자경종 2.2 kg보다 0.1 kg이 높았으며, 고급홍삼인 천지삼율은 고풍은 16.6%로 대비구인 자경종 9.4%보다 7.2%가 더 높았다. 따라서 고풍은 고급 홍삼제조용 원료삼 품종으로 가능성이 매우 높은 것으로 판단되었다.

• Journal of Ginseng Research
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• 제26권2호
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• pp.85-88
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• 2002

고려인삼을 순계분리 육성하여 품종 등록한 인삼 신품종의 광도, 온도별 광합성 능력과 광합성 관련 형질인 기공, 엽록소, 비엽중 및 지상하부 형질들을 조사한 결과를 요약하면 다음과 같다. 신품종 및 자경종의 광포화점은 15,000 lux내외였고, 광합성 최적온도는 2$0^{\circ}C$였다. 광합성 능은 연풍>천풍>고풍>자경종 순으로 연풍이 가장 높았다. 호흡속도는 모든 품종이 온도가 높아짐에 따라 빨라지는 경향이었으며, 타품종에 비해 호흡량이 높았다. 비엽중은 천풍>고풍>연풍>자경종 순서로 높았고, 엽록소 함량은 품종간 차이가 근소하였다. 단위면적당 기공수는 연풍이 69.2개로 타계통의 40~47개보다 많았고, 기공의 크기는 연풍이 26.5 $\mu\textrm{m}$ 타품종 37.5~38.8 $\mu\textrm{m}$보다 적었다. 연풍은 경수가 1.8개, 장염수가 7.7개, 소염수가 41.0개, 엽면적이 12.3 dm$_2$로 타 품종보다 지상부의 생육이 양호하였다. 근중은 연풍>천풍>고풍>자경종 순으로 무거웠고, 동장은 천풍과 고풍이 각각 6.5, 6.8 cm로 자경종 5.6 cm보다 길어서 체형이 양호하였으나 연풍은 동장이 4.4 cm로 짧았다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2005년도 창립30주년기념 추계 학술대회 및 정기총회
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• pp.3-18
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• 2005

고려인삼 8개 신품종의 특성을 요약하면 아래와 같다. 1) 다경발생율이 연풍은 45%로 가장 높았고, 금품은 7%로 가장 낮았으나, 지상부생육은 금품이 가장 양호 하였고, 연풍이 가장 낮았다. 2) 종자결실율은 금품이 85.4%로 가장 높았으며, 천풍이 69.1%로 가장낮았다. 종자수확량은 연풍이 108.3개로 가장많았고, 천풍이 77.5개로 가장 적었다. 3) 엽소발생율은 천풍이 타품종보다 각년생에서 높았으나 연풍은 다소 낮았다. 4) 뿌리 부위별 무게분포비율에서 동체의 비율이 천풍 등 5품종은 49%-55.9%로 자경재래종 63,1%보다 낮았으나, 지근의 비율은 5개 신품종이 19,3%-23.3%로 자경재래종 13.2% 보다 높았고, 세근은 차이가 없었다. 5) 수량은 연풍>금풍>고풍>천풍=선풍>자경재래증 순으로 연풍이 가장 높았고,동장은 천풍이 8.0cm로 가장 길었으나 연풍이 6.4cm로 가장 짧았다. 적변삼 발생율은 신품종이 0.2-9,5%로 자경재래종 16.3%보다 현저히 낮았다. 6) 천삼의 생출율은 천풍>금풍>고풍>선풍>연풍>청선>자경재래종의 순서로 천풍이 높았으며, 천지삼의 생출율도 같은 경향이었다. 7) 품종별 동체의 단위무게 당 total ginsenosides 함량은 금풍 8.53mg, 연풍8.13mg, 고풍7.47mg로 높았으나, 천풍과 선풍은 각각 5.73mg과 4.87mg로 다소낮았다.

• 한국약용작물학회지
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• 제19권3호
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• pp.185-190
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• 2011

The principal objective of this study was to develop a discrimination method using SSR markers in Korean ginseng cultivars. Five cultivars--Chunpoong, Yunpoong, Gopoong, Sunpoong, and Kumpoong--were evaluated by nine markers out of 22 SSR markers. A total of 23 alleles were detected, ranging from 1 to 4, with an average of 2.6 alleles per locus, and an averages of gene diversity (GD) of 0.480. Nine markers were tested in order to distinguish among five Korean ginseng cultivars. Two markers out of nine SSR markers, GB-PG-065 and GB-PG-142, were selected as key markers for discrimination among Korean ginseng cultivars. Two genotypes were detected in GB-PG-065. Chunpoong and Kumpoong shared the same allele type, and Yunpoong, Gopoong, and Sunpoong shared another identical allele type. In the case of GB-PG-142, a specific allele type differentiated from those of other four cultivars was observed only in Sunpoong cultivar. Consequently, the SSR markers developed in this study may prove useful for the identification of Korean ginseng cultivars and the development of ginseng seed management systems, as well as tests to guarantee the purity of ginseng seeds.

• 한국약용작물학회지
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• 제20권5호
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• pp.387-392
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• 2012

This study was carried out to identify Korean ginseng cultivars using peptide nucleic acid (PNA) microarray. Sixty-seven probes were designed based on nucleotide variation to distinguish Korean ginseng cultivars of Panax ginseng. Among those PNA probes, three (PGB74, PGB110 and PGB130) have been developed to distinguish five Korean ginseng cultivars. Five Korean ginseng cultivars were denoted as barcode numbers depending on their fluorescent signal patterns of each cultivar using three probe sets in the PNA microarray. Five Korean ginseng cultivars, Chunpoong, Yunpoong, Gopoong, Gumpoong and Sunpoong, were simply denoted as '111', '222', '211', '221' and '122', respectively. This is the first report of PNA microarray which provided an objective and reliable method for the authentication of Korean ginseng cultivars. Also, the PNA microarray will be useful for management system and pure guarantee in ginseng seed.

• Journal of Ginseng Research
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• 제36권3호
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• pp.298-307
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• 2012

Panax ginseng has been cultivated for centuries, and nine commercial cultivars have been registered in Korea. However, these nine elite cultivars are grown in less than 10% of ginseng fields, and there is no clear authentication system for each cultivar even though their values are higher than those of local landraces. Here, we have developed 19 microsatellite markers using expressed gene sequences and established an authentication system for all nine cultivars. Five cultivars, 'Chunpoong', 'Sunpoong', 'Gumpoong', 'Sunun', and 'Sunone', can each be identified by one cultivar-unique allele, gm47n-a, gm47n-c, gm104-a, gm184-a (or gm129-a), and gm175-c, respectively. 'Yunpoong' can be identified by the co-appearance of gm47n-b and gm129-c. 'Sunhyang' can be distinguished from the other eight cultivars by the co-appearance of gm47n-b, gm129-b, and gm175-a. The two other cultivars, 'Gopoong' and 'Cheongsun', can be identified by their specific combinations of five marker alleles. This marker set was successfully utilized to identify the cultivars among 70 ginseng individuals and to select true F1 hybrid plants between two cultivars. We further analyzed the homogeneity of each cultivar and phylogenetic relationships among cultivars using these markers. This marker system will be useful to the seed industry and for breeding of ginseng.

• Journal of Ginseng Research
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• 제35권4호
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• pp.504-513
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• 2011

In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.

• Journal of Ginseng Research
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• 제35권4호
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• pp.399-412
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• 2011

Little is known about the genetics or genomics of Panax ginseng. In this study, we developed 70 expressed sequence tagderived polymorphic simple sequence repeat markers by trials of 140 primer pairs. All of the 70 markers showed reproducible polymorphism among four Panax species and 19 of them were polymorphic in six P. ginseng cultivars. These markers segregated 1:2:1 manner of Mendelian inheritance in an $F_2$ population of a cross between two P. ginseng cultivars, 'Yunpoong' and 'Chunpoong', indicating that these are reproducible and inheritable mappable markers. A phylogenetic analysis using the genotype data showed three distinctive groups: a P. ginseng-P. japonicus clade, P. notoginseng and P. quinquefolius, with similarity coefficients of 0.70. P. japonicus was intermingled with P. ginseng cultivars, indicating that both species have similar genetic backgrounds. P. ginseng cultivars were subdivided into three minor groups: an independent cultivar 'Chunpoong', a subgroup with three accessions including two cultivars, 'Gumpoong' and 'Yunpoong' and one landrace 'Hwangsook' and another subgroup with two accessions including one cultivar, 'Gopoong' and one landrace 'Jakyung'. Each primer pair produced 1 to 4 bands, indicating that the ginseng genome has a highly replicated paleopolyploid genome structure.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
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• pp.11-11
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• 2010

The Korean ginseng, Panax ginseng C. A. Meyer is a popular medicinal herb in Araliaceae. Genetic map in crops provides valuable information for breeding, genetic and genomic researches. However, little information is available for construction of genetic map in ginseng. Up to now, we have produced large amounts of expressed sequence tags (ESTs) from four ginseng cultivars (37Mb, 49Mb, 39Mb, 47Mb from Gopoong, Gumpoong, Chunpoong and Yunpoong respectively using pyrosequencing technique and 5Mb from normalized full-length cDNA library of Chunpoong) to obtain comprehensive information of gene expression, and constructed EST database including ESTs from public database. Till now, we designed 261 SSR primer sets using EST sequences and identified 106 intergenic polymorphic markers. And 44 of the 106 showed polymorphisms among panax ginseng cultivars. Among 44 markers, 27 SSR polymorphic markers were inspected to 51 $F_2$ population from Yunpoong x Chunpoong, which showed good at the fitness of Mendellian segregation ratio 1:2:1. To enrich the number of markers, and thus construct high resolution genetic map which can be used as frame map for further genome sequencing. we are planning to develop large scale EST-derived SNP markers which are available in the F2 population. This study provides genetic information as well as foundation for ginseng researches such as genetics, genomics, breeding, and the final goal for whole genome sequencing. This study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Grant No. 609001-051SB210).

• 한국약용작물학회지
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• 제21권5호
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• pp.372-379
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• 2013

The present study was investigated the effect of planting density on plant growth and yield of Panax ginseng C. A. Meyer. Sowing density is one of the most important factors affecting yield. The value of roots have affected by shape, color, weight and degree of disease injury in ginseng. Also, it needed to minimize elapsed time for 5 years including pre-planting field management and cultivation period. We were conducted to evaluate that planting densities and varieties on the growth, yield and missing rate. The direct sowing was treated seeds density as a four levels (seeds; 72, 90, 120, 144) and 10 different varieties with 3 years old ginseng roots. Root weight was significantly affected by planting density and variety, but the number of lateral root and yield were affected by only planting density. Growth index was related to variety and planting density. Also, Root shape index was affected by both varieties and planting densities. Suitable planting density and variety were 120 plant per 1.62 $m^2$ and Gopoong, respectively. Results showed that it was also a significant difference (p=5%) in variety of planting density on growth.