Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Molecular Identification of Korean Ginseng Cultivars (Panax ginseng C. A. Mey.) using Peptide Nucleic Acid (PNA) Microarray

PNA 바이오칩을 이용한 고려인삼 품종 판별

  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박홍우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 신미란 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영배 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김옥태 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2012.08.16
  • Accepted : 2012.10.09
  • Published : 2012.10.30
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Abstract

This study was carried out to identify Korean ginseng cultivars using peptide nucleic acid (PNA) microarray. Sixty-seven probes were designed based on nucleotide variation to distinguish Korean ginseng cultivars of Panax ginseng. Among those PNA probes, three (PGB74, PGB110 and PGB130) have been developed to distinguish five Korean ginseng cultivars. Five Korean ginseng cultivars were denoted as barcode numbers depending on their fluorescent signal patterns of each cultivar using three probe sets in the PNA microarray. Five Korean ginseng cultivars, Chunpoong, Yunpoong, Gopoong, Gumpoong and Sunpoong, were simply denoted as '111', '222', '211', '221' and '122', respectively. This is the first report of PNA microarray which provided an objective and reliable method for the authentication of Korean ginseng cultivars. Also, the PNA microarray will be useful for management system and pure guarantee in ginseng seed.

Keywords

References

  1. Bang KH, Chung JW, Kim YC, Lee JW, Jo IH, Seo AY, Kim OT, Hyun DY, Kim DH and Cha SW. (2011a). Development of SSR markers for identification of Korean ginseng (Panax ginseng C. A. Mey.) cultivars. Korean Journal of Medicinal Crop Science. 19:185-190. https://doi.org/10.7783/KJMCS.2011.19.3.185
  2. Bang KH, Jo IH, Chung JW, Kim YC, Lee JW, Seo AY, Park JH, Kim OT, Hyun DY, Kim DH and Cha SW. (2011b). Analysis of genetic polymorphism of Korean ginseng cultivars and foreign accessions using SSR markers. Korean Journal of Medicinal Crop Science. 19:347-353. https://doi.org/10.7783/KJMCS.2011.19.5.347
  3. Bang KH, Lee JW, Kim YC, Kim DH, Lee EH and Jeung JU. (2010). Construction of genomic DNA library of Korean ginseng (Panax ginseng C. A. Meyer) and development of sequence-tagged sites. Biological and Pharmaceutical Bulletin. 33:1579-1588. https://doi.org/10.1248/bpb.33.1579
  4. Choi JE, Seo SD, Yuk JA, Cha SK, Kim HH, Seong BJ and Kim SL. (2003). Analysis of diversity of Panax ginseng collected in Korea by RAPD technique. Korean Journal of Medicinal Crop Science. 11:377-384.
  5. Choi JJ, Kim CH and Park HK. (2009). Peptide nucleic acidbased array for detecting and genotyping human papilomaviruses. Journal of Clinical Microbiology. 47:1785-1790. https://doi.org/10.1128/JCM.01398-08
  6. Choi KT and Shin HS. (1982). Morphological characteristics of inflorescence, flowering bud, fruit and leaf of Korean ginseng. Journal of Ginseng Research. 6:67-74.
  7. Dan NV, Ramchiary N, Choi SR, Uhm TS, Yang TJ, Ahn IO and Lim YP. (2009). Development and characterization of new microsatellite markers in Panax ginseng C. A. Meyer from BAC end sequences. Conserved Genetics. 11:1223-1225.
  8. Fushimi H, Komatsu K, Isobe M and Namba T. (1997). Application of PCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three Ginseng drugs. Biological and Pharmaceutical Bulletin. 20:765-769. https://doi.org/10.1248/bpb.20.765
  9. Hu SY. (1976). The genus Panax (ginseng) in Chinese medicine. Economic Botany. 30:11-28. https://doi.org/10.1007/BF02866780
  10. Kim OT, Bang KH, In DS, Lee JW, Kim YC, Shin YS, Hyun DY, Lee SS, Cha SW and Seong NS. (2007). Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences. Plant Biotechnology Reports. 1:163-167. https://doi.org/10.1007/s11816-007-0019-2
  11. Kim YC. (2012). Classification of the morphological characteristics of ginseng (Panax ginseng C. A. Meyer) germplasm and selection of useful resources. Ph. D. Thesis. Chungnam National University. p.4-7.
  12. Kwon WS, Chung CM, Kim YT and Choi KT. (1991). Comparisons of growth, crude saponin, ginsenosides, and anthocyanins in superior lines of Panax ginseng C. A. Meyer. Korean Journal of Breeding. 23:219-228.
  13. Kwon WS, Chung CM, Kim YT, Lee MG and Choi KT. (1998). Breeding process and characteristics of KG101, a superior line of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 22:11-17.
  14. Kwon WS, Lee JH, Park CS and Yang DC. (2003). Breeding process and characteristics of Gopoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 27:86-91. https://doi.org/10.5142/JGR.2003.27.2.086
  15. Kwon WS, Lee MG and Choi KT. (2000). Breeding process and characteristics of Yunpoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 24:1-7.
  16. Lee JW. (2010). Development of DNA markers for identification of Korean ginseng cultivars (Panax ginseng C. A. Meyer). Ph. D. Thesis. Dongguk University. p.20-81.
  17. Lee JW, Bang KH, Choi JJ, Chung JW, Lee JH, Jo IH, Seo AY, Kim YC, Kim OT and Cha SW. (2010). Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions. Genes and Genomics. 32:463-468. https://doi.org/10.1007/s13258-010-0040-7
  18. Lim YP, Shin CS, Lee SJ, Yoon YN and Jo JS. (1993). Survey of proper primers and genetic analysis of Korean ginseng (Panax ginseng C. A. Meyer) variants using the RAPD technique. Journal of Ginseng Research. 17:153-158.
  19. Ngan F, Shaw P, But P and Wang J. (1999). Molecular authentication of Panax species. Phytochemistry. 50:787-791. https://doi.org/10.1016/S0031-9422(98)00606-2
  20. Nielsen PE, Egholm M and Buchardt O. (1994). Sequence specific transcription arrest by PNA bound to the template strand. Gene. 149:139-145. https://doi.org/10.1016/0378-1119(94)90422-7
  21. Park HG, Ji HS, Yim SC and Chang HN. (2002). DNA chip and immobilization technologies. Korean Chemical Engineering Research. 40:281-288.
  22. Pellestor F, Paulasova P and Hamamah S. (2008). Peptide nucleic acids (PNAs) as diagnostic devices for genetic and cytogenetic analysis. Current Pharmaceutical Design. 14:2439-2444. https://doi.org/10.2174/138161208785777405
  23. Rural Development Administration. (2009). Standard cultivation guidebook for good agricultural practice. Rural Development Administration. Suwon, Korea. p.47-117.
  24. Sforza S, Corradini R, Tedeschi T and Marchelli R. (2011). Food analysis and food authentication by peptide nucleic acid (PNA)-based technologies. Chemical Society Reviews. 40:221-232. https://doi.org/10.1039/b907695f
  25. Shaw PC and But PP. (1995). Authentication of Panax species and their adulterants by random-primed polymerase chain reaction. Planta Medica. 61:466-469. https://doi.org/10.1055/s-2006-958138
  26. Singh RP, Oh BK and Choi JW. (2010). Application of peptide nucleic acid towards development of nanobiosensor arrays. Bioelectrochemistry. 79:153-161. https://doi.org/10.1016/j.bioelechem.2010.02.004
  27. Wen J and Zimmer EA. (1996). Phylogeny and biogeography of Panax L. (the ginseng genus, Araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution. 6:167-177. https://doi.org/10.1006/mpev.1996.0069
  28. Yang DC, Yang KJ and Yoon ES. (2001). Comparison of ITS (internal transcribed spacer) and 5.8S rDNA sequences among varieties and cultivars in Panax ginseng. Journal of Photoscience. 8:55-60.

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  1. Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species vol.23, pp.6, 2015, https://doi.org/10.7783/KJMCS.2015.23.6.439
  2. Applications of molecular markers in the discrimination of Panax species and Korean ginseng cultivars ( Panax ginseng ) 2017, https://doi.org/10.1016/j.jgr.2016.09.001